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Original Articles

THE USE AND EVALUATION OF 2+2 PHOTOADDITION IN IMMOBILIZATION OF OLIGONUCLEOTIDES ON A THREE DIMENSIONAL HYDROGEL MATRIX

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Pages 1371-1375 | Published online: 07 Feb 2007

REFERENCES

  • Gushin , D. , Yershov , G. , Zaslavsky , A. , Gemmell , A. , Shick , V. , Prodnikov , D. , Arenkov , P. and Mirzabekov , A. 1997 . Analytical Biochemistry , 250 : 203 – 211 . and references there in
  • Vasiliskov , A. V. , Timofeev , E. N. , Surzhikov , S. A. , Drobyshev , A. L. , Shick , V. V. and Mirzabekov , A. 1999 . BioTechniques , 27 : 592 – 606 .
  • Cheng , J. , Sheldon , S. L. , Wu , L. , Uribe , A. , Gerrue , L. O. , Carrino , J. and Heller , M. J. 1998 . Nat. Biotechnol. , 16 : 541 – 546 .
  • We found very little difference between the performance of the cinnamide or methacrylamide oligonucleotides in the photoattachment chemistry. However, due to the inherent hydrophobicity of the cinnamide group purification of these oligos are more convenient. Further details of the photochemistry of the hydrogel, synthesis of the cinnamide oligos and the details of the assays used will be provided in follow up publications to this work
  • Finter , J. , Haniotis , Z. , Lohse , F. , Meier , K. and Zweifel , H. 1985 . Angew. Makromol. Chem. , 133 : 147 – 170 .

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