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Original Articles

Purification of MBP-β-galactosidase and MBP-rubredoxin through affinity membrane separation

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Pages 1699-1724 | Published online: 15 Feb 2007

REFERENCES

  • Wheelwright , S.M. 1989 . The Design of Downstream Processes for Large-Scale Protein Purification . J. Biotechnol. , 11 : 89 – 102 .
  • Labrou , N. and Clonis , Y.D. 1994 . The Affinity Technology in Downstream Processing . J. Biotechnol. , 36 : 95 – 119 .
  • Sada , E. 1990 . Engineering Aspects of Bioaffinity Separation . J. Chem. Eng. Jpn , 23 : 259 – 269 .
  • Charcosset , C. 1998 . Purification of Proteins by Membrane . Chromatogr. J. Chem. Technol. Biotechnol. , 71 : 95 – 110 .
  • Klein , E. 1991 . Affinity Membranes, Their Chemistry and Performance in Adsorptive Separation Processes New York : Wiley .
  • Unarska , M. , Davies , P.A. , Esnouf , M.P. and Bellhouse , B.J. 1990 . Comparative Study of Reaction Kinetics in Membrane and Agarose Bead Affinity Systems . J. Chromatogr. , 519 : 53 – 67 .
  • Briefs , K.G. and Kula , M.R. 1992 . Fast Protein Chromatography on Analytical and Preparative Scale Using Modified Microporous Membranes . Chem. Eng. Sci. , 47 : 141 – 149 .
  • Gerstner , J.A. , Hamilton , R. and Cramer , S.M. 1992 . Membrane Chromatographic System for High-Throughput Protein Separation . J. Chromatogr. , 596 : 173 – 180 .
  • Maina , C. , Riggs , P.D. , Grandea , A.G. III , Slatko , B.E. , Moran , L.S. , Tagliamonte , J.A. , McReynolds , L.A. and Guan , C. 1988 . An Escherichia coli Vector to Express and Purify Foreign Proteins by Fusion and Separation from Maltose-Binding Protein . Gene , 74 : 365 – 373 .
  • Guan , C. , Li , P. , Riggs , P.D. and Inouye , H. 1988 . Vectors That Facilitate the Expression and Purification of Foreign Peptides in Escherichia coli by Fusion to Maltose-Binding Protein . Gene , 67 : 21 – 30 .
  • in press .
  • Steers , E. and Cuatrecasas , P. 1974 . β-Galactosidase . Methods Enzymol. , 34 : 350 – 358 .
  • Lovenberg , W. and Walker , M.N. 1978 . Rubredoxin . Methods Enzymol. , 53 : 340 – 346 .
  • Steers , E. , Cuatrecasas , P. and Pollard , H.B. 1971 . The Purification of β-Galactosidase from Escherichia coli by Affinity Chrmoatography . J. Biol. Chem. , 246 : 196 – 200 .
  • in press .
  • Miller , J.H. 1972 . Experiments in Molecular Genetics Edited by: Miller , J.H. 352 – 355 . Cold Spring Harbor : Cold Spring Harbor Laboratory .
  • Bradford , M.M. 1976 . A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilising the Principle of Protein–Dye Binding . Anal. Biochem. , 72 : 248 – 254 .
  • Sedmak , J.J. and Grossberg , S.E. 1977 . A Rapid, Sensitive and Versatile Assay for Protein Using Coomassie Brilliant Blue G-250 . Anal. Biochem. , 79 : 544 – 552 .
  • Kellerman , O.K. and Ferenci , T. 1982 . Maltose-Binding Protein from E. coli . Methods Enzymol. , 90 : 459 – 465 .
  • Sundberg , L. and Porath , J. 1974 . Preparation of Adsorbent for Biospecific Affinity Chromatography. I. Attachment of Group-Containing Ligands to Insoluble Polymers by Means of Bifunctional Oxiranes . J. Chromatgr. , 90 : 87 – 98 .
  • Luby , P. and Kuniak , L. 1979 . Crosslinking Statistics 2a) Relative Reactivities of Amylose Hydroxyl Groups . Makromol. Chem. , 180 : 2213 – 2220 .
  • in press .
  • Kellermann , O.K. and Szmelchamnn , S. 1974 . Active Transport of Maltose in Escherichia coli K12 . Eur. J. Biochem. , 47 : 139 – 149 .
  • Szmelchmann , S. , Schwartz , M. , Silhavy , J. and Boos , W. 1976 . Maltose Transport in Escherichia coli K12 . Eur. J. Biochem. , 71 : 167 – 170 .
  • Silhavy , T.J. , Szmelcmann , S. , Boos , W. and Schwartz , M. 1975 . On the Significance of the Retention of Ligand by Protein . Proc. Natl Acad. Sci. USA , 72 ( 6 ) : 2120 – 2124 .
  • Srinivasan , U. and Bell , J.A. 1998 . A Convenient Method for Affinity Purification of Maltose Binding Protein Fusions . J. Biotechnol. , 62 : 163 – 167 .

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