Image-Based Cytometric Analysis of Fluorescent Viability and Vitality Staining Methods for Ale and Lager Fermentation Yeast

Literature Cited

• Aabo, T., Glückstad, J., Siegumfeldt, H., and Arneborg, N. Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae. J. Royal Soc. Interface. 8:1635–1643, 2011.
   Web of Science ®Google Scholar
• Autio, K., and Mattila-Sandholm, T. Detection of active yeast cells (Saccharomyces cerevisiae) in frozen dough sections. Appl. Environ. Microbiol. 58:2153–2157, 1992.
   Web of Science ®Google Scholar
• Chan, L. L.-Y., Kuksin, D., Laverty, D. J., Saldi, S., and Qiu, J. Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method. Cytotechnology DOI:10.1007/s10616-014-9704-5, 2014.
   Google Scholar
• Chan, L. L., Kury, A., Wilkinson, A., Berkes, C., and Pirani, A. Novel image cytometric method for detection of physiological and metabolic changes in Saccharomyces cerevisiae. J. Ind. Microbiol. Biotech. 39:1615–1623, 2012.
   PubMed Web of Science ®Google Scholar
• Chan, L. L., Lyettefi, E. J., Pirani, A., Smith, T., Qiu, J., and Lin, B. Direct concentration and viability measurement of yeast in corn mash using a novel imaging cytometry method. J. Ind. Microbiol. Biotech. 38:1109–1115, 2010.
   Web of Science ®Google Scholar
• Chan, L. L., Wilkinson, A. R., Paradis, B. D., and Lai, N. Rapid image-based cytometry for comparison of fluorescent viability staining methods. J. Fluoresc. 22:1301–1311, 2012.
   PubMed Web of Science ®Google Scholar
• Corliss, D. A., and White, W. E. Jr. Fluorescence of yeast vitally stained with ethidium bromide and propidium iodide. J. Histochem. Cytochem. 29:45–48, 1981.
   PubMed Web of Science ®Google Scholar
• Hernlem, B., and Hua, S.-S. Dual fluorochrome flow cytometric assessment of yeast viability. Curr. Microbiol. 61:57–63, 2010.
   PubMed Web of Science ®Google Scholar
• Imai, T., Nakajima, I., and Ohno, T. Development of a new method for evaluation of yeast vitality by measuring intracellular pH. J. Am. Soc. Brew. Chem. 52:5–8, 1994.
   Web of Science ®Google Scholar
• Jenkins, D. M., Powell, C. D., Fischborn, T., and Smart, K. A. Rehydration of active dry brewing yeast and its effect on cell viability. J. Inst. Brew. 117:377–382, 2011.
   Web of Science ®Google Scholar
• Laverty, D. J., Kury, A. L., Kuksin, D., Pirani, A., Flanagan, K., and Chan, L. L.-Y. Automated quantification of budding Saccharomyces cerevisiae using a novel image cytometry method. J. Ind. Microbiol. Biotech. 40:581–588, 2013.
   PubMed Web of Science ®Google Scholar
• Li, W., Guo, Z., Zhang, L., and Ding, Z. A novel and rapid method for yeast vitality evaluation based on the methylene blue dye reduction test. J. Am. Soc. Brew. Chem. 69:44–49, 2011.
   Web of Science ®Google Scholar
• Lloyd, D., and Dinsdale, G. From brightfield to fluorescence and confocal microscopy. In: Brewing Yeast Fermentation Performance. K. A. Smart, ed. Blackwell Science, Oxford. Pp. 3–9, 2000.
   Google Scholar
• McCaig, R. Evaluation of the fluorescent dye 1-anilino-8-naphthalene sulfonic acid for yeast viability determination. J. Am. Soc. Brew. Chem. 48:22–25, 1990.
   Google Scholar
• Raschke, D., and Knorr, D. Rapid monitoring of cell size, vitality, and lipid droplet development in the oleaginous yeast Waltomyces lipofer. J. Microbiol. Meth. 79:178–183, 2009.
   Web of Science ®Google Scholar
• Sami, M., Ikeda, M., and Yabuuchi, S. Evaluation of the alkaline methylene blue staining method for yeast activity determination. J. Ferm. Bioeng. 78:212–216, 1994.
   Google Scholar
• Slater, M. L. Rapid nuclear staining method for Saccharomyces cerevisiae. J. Bacteriol. 126:1339–1341, 1976.
   PubMed Web of Science ®Google Scholar
• Slaughter, J. C., and Nomura, T. Intracellular glycogen and trehalose contents as predictors of yeast viability. Enzyme. Microbol. Technol. 14:64–67, 1992.
   Web of Science ®Google Scholar
• Smart, K. A., Chambers, K. M., Lambert, I., Jenkins, C., and Smart, C. A. Use of methylene violet staining procedure to determine yeast viability and vitality. J. Am. Soc. Brew. Chem. 57:18–23, 1999.
   Web of Science ®Google Scholar
• Thiele, F., and Back, W. Measurement of yeast vitality using a modified version of the intracellular pH measurement (ICP). Monatsschr. Brauwiss. 58:2–5, 2005.
   Google Scholar
• Van Zandycke, S. M., Simal, O., Gualdoni, S., and Smart, K. A. Determination of yeast viability using fluorophores. J. Am. Soc. Brew. Chem. 61:15–22, 2003.
   Web of Science ®Google Scholar
• Weigert, C., Steffler, F., Kurz, T., Shellhammer, T. H., and Methner, F.-J. Application of a short intracellular pH method to flow cytometry for determining Saccharomyces cerevisiae vitality. Appl. Environ. Microbiol. 75:5615–5620, 2009.
   PubMed Web of Science ®Google Scholar