40
Views
13
CrossRef citations to date
0
Altmetric
Article

A Weak Spliceosome-Binding Domain of Yju2 Functions in the First Step and Bypasses Prp16 in the Second Step of Splicing

&
Pages 1746-1755 | Received 09 Jan 2013, Accepted 07 Feb 2013, Published online: 20 Mar 2023

REFERENCES

  • Brow DA. 2002. Allosteric cascade of spliceosome activation. Annu. Rev. Genet. 36:333–360.
  • Wahl MC, Will CL, Lührmann RL. 2009. The spliceosome: design principles of a dynamic RNP machine. Cell 136:701–718.
  • Will CL, Lührmann R. 2011. Spliceosome structure and function. Cold Spring Harb. Perspect. Biol. 3:a003707. doi:10.1101/cshperspect.a003707.
  • Chen H-C, Cheng S-C. 2012. Functional roles of protein splicing factors. Biosci. Rep. 32:345–359.
  • Tarn W-Y, Hsu C-H, Huang K-T, Chen H-R, Kao H-Y, Lee K-R, Cheng S-C. 1994. Functional association of essential splicing factor(s) with PRP19 in a protein complex. EMBO J. 13:2421–2431.
  • Chan S-P, Cheng S-C. 2005. The Prp19-associated complex is required for specifying interactions of U5 and U6 with pre-mRNA during spliceosome activation. J. Biol. Chem. 280:31190–31199.
  • Chan S-P, Kao D-I, Tsai W-Y, Cheng S-C. 2003. The Prp19p-associated complex in spliceosome activation. Science 302:279–282.
  • Warkocki Z, Odenwälder P, Schmitzová J, Platzmann F, Stark H, Urlaub H, Ficner R, Fabrizio P, Lührmann R. 2009. Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components. Nat. Struct. Mol. Biol. 16:1237–1243.
  • Staley JP, Guthrie C. 1998. Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell 92:315–326.
  • Lardelli RM, Thompson JX, Yates JRIII, Stevens SW. 2010. Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing. RNA 16:516–528.
  • Ohrt T, Prior M, Dannenberg J, Odenwalder P, Dybkov O, Rasche N, Schmitzova J, Gregor I, Fabrizio P, Enderiein J, Lührmann R. 2012. Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed dcFCCS. RNA 18:1244–1256.
  • Gozani O, Potashkin J, Reed R. 1998. A potential role for U2AF SAP155 interactions in recruiting U2 snRNP to the branch site. Mol. Cell. Biol. 18:4752–4760.
  • McPheeters DS, Muhlenkamp P. 2003. Spatial organization of protein-RNA interactions in the branch site-3′ splice site region during pre-mRNA splicing in yeast. Mol. Cell. Biol. 23:4174–4186.
  • Liu Y-C, Chen H-C, Wu N-Y, Cheng S-C. 2007. A novel splicing factor Yju2 is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing. Mol. Cell. Biol. 27:5403–5413.
  • Chiu Y-F, Liu Y-C, Chiang T-W, Yeh T-C, Tseng C-K, Wu NY, Cheng S-C. 2009. Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction. Mol. Cell. Biol. 29:5671–5678.
  • Tseng C-K, Liu H-L, Cheng S-C. 2011. DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps. RNA 17:145–154.
  • Frank D, Guthrie C. 1992. An essential splicing factor, SLU7, mediates 3′ splice site choice in yeast. Genes Dev. 6:2112–2124.
  • Ansari A, Schwer B. 1995. SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing. EMBO J. 14:4001–4009.
  • Horowitz DS, Abelson J. 1993. Stages in the second reaction of pre-mRNA splicing: the final step is ATP independent. Genes Dev. 7:320–329.
  • Schwer B, Gross CH. 1998. Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J. 17:2086–2094.
  • Raghunathan PL, Guthrie C. 1998. RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2. Curr. Biol. 8:847–855.
  • Laggerbauer B, Achsel T, Lührmann R. 1998. The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro. Proc. Natl. Acad. Sci. U. S. A. 95:4188–4192.
  • Wagner JDO, Jankowsky E, Company M, Pyle AM, Abelson JN. 1998. The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes. EMBO J. 17:2926–2937.
  • Wang Y, Guthrie C. 1998. PRP16, a DEAH-box RNA helicase, is recruited to the spliceosome primarily via its nonconserved N-terminal domain. RNA 4:1216–1229.
  • Tanaka N, Schwer B. 2006. Mutations in PRP43 that uncouple RNA-dependent NTPase activity and pre-mRNA splicing function. Biochemistry 45:6510–6521.
  • Jankowsky E, Bowers H. 2006. Remodeling of ribonucleoprotein complexes with DExH/D RNA helicases. Nucleic Acids Res. 34:4181–4188.
  • Jankowsky E, Cross CH, Shuman S, Pyle AM. 2001. Active disruption of an RNA-protein interaction by a DExH/D RNA helicase. Science 291:121–125.
  • Fairman ME, Maroney PA, Wang W, Bowers HA, Gollnick P, Nilsen TW, Jankowsky E. 2004. Protein displacement by DExH/D “RNA helicases” without duplex unwinding. Science 304:730–734.
  • Kistler AL, Guthrie C. 2001. Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase. Genes Dev. 15:42–49.
  • Perriman R, Barta I, Voeltz GK, Abelson J, Ares MJr. 2003. ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA. Proc. Natl. Acad. Sci. U. S. A. 100:13857–13862.
  • Chen JY-F, Stands L, Staley JP, Jackups RRJr, Latus LJ, Chang T-H. 2001. Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor. Mol. Cell 7:227–232.
  • Company M, Arenas J, Abelson J. 1991. Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes. Nature 349:487–493.
  • Schwer B. 2008. A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release. Mol. Cell 30:743–754.
  • Liu H-L, Cheng S-C. 2012. The Interaction of Prp2 with a defined Region of the intron is required for the first splicing reaction. Mol. Cell. Biol. 32:5056–5066.
  • Mefford MM, Staley JP. 2009. Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps. RNA. 15:1386–1397.
  • Chang K-J, Chen H-C, Cheng S-C. 2009. Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation. RNA 15:1729–1739.
  • Cheng S-C, Newman A, Lin R-J, McFarland GD, Abelson JN. 1990. Preparation and fractionation of yeast splicing extract. Methods Enzymol. 181:89–96.
  • Cheng S-C, Abelson J. 1987. Spliceosome assembly in yeast. Genes Dev. 1:1014–1027.
  • Schneider S, Hotz H-R, Schwer B. 2002. Characterization of dominant-negative mutants of the DEAH-box splicing factors Prp22 and Prp16. J. Biol. Chem. 277:15452–15458.
  • Dix I, Russell CS, O'Keefe RT, Newman AJ, Beggs JD. 1998. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA 4:1675–1686.
  • Grainger RJ, Beggs JD. 2005. Prp8 protein: At the heart of the spliceosome. RNA 11:533–557.
  • Urlaub H, Hatmuth K, Kostka S, Grelle G, Lührmann R. 2000. A general approach for identification of RNA-protein cross-linking sites within native human spliceosomal nuclear ribonucleoproteins (snRNPs). J. Biol. Chem. 275:41458–41468.
  • Vidal VP, Verdone L, Mayes AE, Beggs JD. 1999. Characterization of U6 snRNA-protein interactions. RNA 5:470–1481.
  • Perriman R, Ares MJr. 2010. Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing. Mol. Cell 38:416–427.
  • Wang Y, Wagner JDO, Guthrie C. 1998. The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro. Curr. Biol. 8:441–451.
  • Chen H-C, Tseng C-K, Tsai R-T, Chung C-S, Cheng S-C. 2013. Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16 and Prp22. Mol. Cell. Biol. 33:514–525.
  • Perriman R, Ares MJr. 2007. Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing. Genes Dev. 21:811–820.
  • Hilliker AK, Mefford MA, Staley JP. 2007. U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing. Genes Dev. 21:821–834.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.