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Research Article

Ubiquitylation of Ku80 by RNF126 Promotes Completion of Nonhomologous End Joining-Mediated DNA Repair

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Article: e00347-16 | Received 15 Jun 2016, Accepted 22 Nov 2016, Published online: 17 Mar 2023

REFERENCES

  • Heyer WD, Ehmsen KT, Liu J. 2010. Regulation of homologous recombination in eukaryotes. Annu Rev Genet 44:113–139. https://doi.org/10.1146/annurev-genet-051710-150955.
  • Lieber MR. 2010. The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway. Annu Rev Biochem 79:181–211. https://doi.org/10.1146/annurev.biochem.052308.093131.
  • Postow L. 2011. Destroying the ring: freeing DNA from Ku with ubiquitin. FEBS Lett 585:2876–2882. https://doi.org/10.1016/j.febslet.2011.05.046.
  • Postow L, Ghenoiu C, Woo EM, Krutchinsky AN, Chait BT, Funabiki H. 2008. Ku80 removal from DNA through double strand break-induced ubiquitylation. J Cell Biol 182:467–479. https://doi.org/10.1083/jcb.200802146.
  • Postow L, Funabiki H. 2013. An SCF complex containing Fbxl12 mediates DNA damage-induced Ku80 ubiquitylation. Cell Cycle 12:587–595. https://doi.org/10.4161/cc.23408.
  • Ismail IH, Gagne JP, Genois MM, Strickfaden H, McDonald D, Xu Z, Poirier GG, Masson JY, Hendzel MJ. 2015. The RNF138 E3 ligase displaces Ku to promote DNA end resection and regulate DNA repair pathway choice. Nat Cell Biol 17:1446–1457. https://doi.org/10.1038/ncb3259.
  • Feng L, Chen J. 2012. The E3 ligase RNF8 regulates KU80 removal and NHEJ repair. Nat Struct Mol Biol 19:201–206. https://doi.org/10.1038/nsmb.2211.
  • Brown JS, Lukashchuk N, Sczaniecka-Clift M, Britton S, le Sage C, Calsou P, Beli P, Galanty Y, Jackson SP. 2015. Neddylation promotes ubiquitylation and release of Ku from DNA-damage sites. Cell Rep 11:704–714. https://doi.org/10.1016/j.celrep.2015.03.058.
  • Metzger MB, Hristova VA, Weissman AM. 2012. HECT and RING finger families of E3 ubiquitin ligases at a glance. J Cell Sci 125:531–537. https://doi.org/10.1242/jcs.091777.
  • Li W, Bengtson MH, Ulbrich A, Matsuda A, Reddy VA, Orth A, Chanda SK, Batalov S, Joazeiro CA. 2008. Genome-wide and functional annotation of human E3 ubiquitin ligases identifies MULAN, a mitochondrial E3 that regulates the organelle's dynamics and signaling. PLoS One 3:e1487. https://doi.org/10.1371/journal.pone.0001487.
  • Koike M, Yutoku Y, Koike A. 2014. Impact of amino acid substitutions in two functional domains of Ku80: DNA-damage-sensing ability of Ku80 and survival after irradiation. J Vet Med Sci 76:51–56. https://doi.org/10.1292/jvms.13-0283.
  • Jin S, Weaver DT. 1997. Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J 16:6874–6885. https://doi.org/10.1093/emboj/16.22.6874.
  • Hjerpe R, Aillet F, Lopitz-Otsoa F, Lang V, England P, Rodriguez MS. 2009. Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities. EMBO Rep 10:1250–1258. https://doi.org/10.1038/embor.2009.192.
  • Chan DW, Ye R, Veillette CJ, Lees-Miller SP. 1999. DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer. Biochemistry 38:1819–1828. https://doi.org/10.1021/bi982584b.
  • Cohen HY, Lavu S, Bitterman KJ, Hekking B, Imahiyerobo TA, Miller C, Frye R, Ploegh H, Kessler BM, Sinclair DA. 2004. Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis. Mol Cell 13:627–638. https://doi.org/10.1016/S1097-2765(04)00094-2.
  • Mayeur GL, Kung WJ, Martinez A, Izumiya C, Chen DJ, Kung HJ. 2005. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem 280:10827–10833. https://doi.org/10.1074/jbc.M413336200.
  • Errami A, Smider V, Rathmell WK, He DM, Hendrickson EA, Zdzienicka MZ, Chu G. 1996. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol Cell Biol 16:1519–1526. https://doi.org/10.1128/MCB.16.4.1519.
  • Lan L, Ui A, Nakajima S, Hatakeyama K, Hoshi M, Watanabe R, Janicki SM, Ogiwara H, Kohno T, Kanno S, Yasui A. 2010. The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell 40:976–987. https://doi.org/10.1016/j.molcel.2010.12.003.
  • Ogiwara H, Ui A, Otsuka A, Satoh H, Yokomi I, Nakajima S, Yasui A, Yokota J, Kohno T. 2011. Histone acetylation by CBP and p300 at double-strand break sites facilitates SWI/SNF chromatin remodeling and the recruitment of non-homologous end joining factors. Oncogene 30:2135–2146. https://doi.org/10.1038/onc.2010.592.
  • Walker JR, Corpina RA, Goldberg J. 2001. Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair. Nature 412:607–614. https://doi.org/10.1038/35088000.
  • Mailand N, Bekker-Jensen S, Faustrup H, Melander F, Bartek J, Lukas C, Lukas J. 2007. RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins. Cell 131:887–900. https://doi.org/10.1016/j.cell.2007.09.040.
  • Huen MS, Grant R, Manke I, Minn K, Yu X, Yaffe MB, Chen J. 2007. RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly. Cell 131:901–914. https://doi.org/10.1016/j.cell.2007.09.041.
  • Kolas NK, Chapman JR, Nakada S, Ylanko J, Chahwan R, Sweeney FD, Panier S, Mendez M, Wildenhain J, Thomson TM, Pelletier L, Jackson SP, Durocher D. 2007. Orchestration of the DNA-damage response by the RNF8 ubiquitin ligase. Science 318:1637–1640. https://doi.org/10.1126/science.1150034.
  • Smith CJ, Berry DM, McGlade CJ. 2013. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. J Cell Sci 126:1366–1380. https://doi.org/10.1242/jcs.116129.
  • Wang Y, Deng O, Feng Z, Du Z, Xiong X, Lai J, Yang X, Xu M, Wang H, Taylor D, Yan C, Chen C, Difeo A, Ma Z, Zhang J. 2015. RNF126 promotes homologous recombination via regulation of E2F1-mediated BRCA1 expression. Oncogene 35:1363–1372. https://doi.org/10.1038/onc.2015.198.
  • Smith CJ, McGlade CJ. 2014. The ubiquitin ligase RNF126 regulates the retrograde sorting of the cation-independent mannose 6-phosphate receptor. Exp Cell Res 320:219–232. https://doi.org/10.1016/j.yexcr.2013.11.013.
  • Rodrigo-Brenni MC, Gutierrez E, Hegde RS. 2014. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell 55:227–237. https://doi.org/10.1016/j.molcel.2014.05.025.
  • Ciccia A, Elledge SJ. 2010. The DNA damage response: making it safe to play with knives. Mol Cell 40:179–204. https://doi.org/10.1016/j.molcel.2010.09.019.
  • Morita S, Kojima T, Kitamura T. 2000. Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7:1063–1066. https://doi.org/10.1038/sj.gt.3301206.
  • Hosogane M, Funayama R, Nishida Y, Nagashima T, Nakayama K. 2013. Ras-induced changes in H3K27me3 occur after those in transcriptional activity. PLoS Genet 9:e1003698. https://doi.org/10.1371/journal.pgen.1003698.
  • Natsume T, Yamauchi Y, Nakayama H, Shinkawa T, Yanagida M, Takahashi N, Isobe T. 2002. A direct nanoflow liquid chromatography-tandem mass spectrometry system for interaction proteomics. Anal Chem 74:4725–4733. https://doi.org/10.1021/ac020018n.
  • Kamura T, Maenaka K, Kotoshiba S, Matsumoto M, Kohda D, Conaway RC, Conaway JW, Nakayama KI. 2004. VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases. Genes Dev 18:3055–3065. https://doi.org/10.1101/gad.1252404.
  • Ishida N, Hara T, Kamura T, Yoshida M, Nakayama K, Nakayama KI. 2002. Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export. J Biol Chem 277:14355–14358. https://doi.org/10.1074/jbc.C100762200.
  • Britton S, Coates J, Jackson SP. 2013. A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. J Cell Biol 202:579–595. https://doi.org/10.1083/jcb.201303073.
  • Beucher A, Birraux J, Tchouandong L, Barton O, Shibata A, Conrad S, Goodarzi AA, Krempler A, Jeggo PA, Lobrich M. 2009. ATM and Artemis promote homologous recombination of radiation-induced DNA double-strand breaks in G2. EMBO J 28:3413–3427. https://doi.org/10.1038/emboj.2009.276.

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