47
Views
12
CrossRef citations to date
0
Altmetric
Article

Functional Analysis of Centrosomal Kinase Substrates in Drosophila melanogaster Reveals a New Function of the Nuclear Envelope Component Otefin in Cell Cycle Progression

, , , , , , , , , , & show all
Pages 3554-3569 | Received 15 Jun 2012, Accepted 25 Jun 2012, Published online: 20 Mar 2023

REFERENCES

  • Aihara H, et al. 2004. Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo. Genes Dev. 18:877–888.
  • Andersen JS, et al. 2003. Proteomic characterization of the human centrosome by protein correlation profiling. Nature 426:570–574.
  • Ashery-Padan R, et al. 1997. Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development. Mol. Cell. Biol. 17:4114–4123.
  • Barr AR, Gergely F. 2007. Aurora-A: the maker and breaker of spindle poles. J. Cell Sci. 120:2987–2996.
  • Basto R, et al. 2008. Centrosome amplification can initiate tumorigenesis in flies. Cell 133:1032–1042.
  • Beaudouin J, Gerlich D, Daigle N, Eils R, Ellenberg J. 2002. Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina. Cell 108:83–96.
  • Berdnik D, Knoblich JA. 2002. Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis. Curr. Biol. 12:640–647.
  • Bettencourt-Dias M, et al. 2004. Genome-wide survey of protein kinases required for cell cycle progression. Nature 432:980–987.
  • Bettencourt-Dias M, Glover DM. 2007. Centrosome biogenesis and function: centrosomics brings new understanding. Nat. Rev. Mol. Cell Biol. 8:451–463.
  • Bjorklund M, et al. 2006. Identification of pathways regulating cell size and cell-cycle progression by RNAi. Nature 439:1009–1013.
  • Blangy A, Arnaud L, Nigg EA. 1997. Phosphorylation by p34cdc2 protein kinase regulates binding of the kinesin-related motor HsEg5 to the dynactin subunit p150. J. Biol. Chem. 272:19418–19424.
  • Blower MD, Feric E, Weis K, Heald R. 2007. Genome-wide analysis demonstrates conserved localization of messenger RNAs to mitotic microtubules. J. Cell Biol. 179:1365–1373.
  • Bluemlein K, Ralser M. 2011. Monitoring protein expression in whole-cell extracts by targeted label- and standard-free LC-MS/MS. Nat. Protoc. 6:859–869.
  • Bodenmiller B, et al. 2007. PhosphoPep—a phosphoproteome resource for systems biology research in Drosophila Kc167 cells. Mol. Syst. Biol. 3:139. https://doi.org/10.1038/msb4100182.
  • Bornens M. 2002. Centrosome composition and microtubule anchoring mechanisms. Curr. Opin. Cell Biol. 14:25–34.
  • Clemens JC, et al. 2000. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl. Acad. Sci. U. S. A. 97:6499–6503.
  • Crasta K, Huang P, Morgan G, Winey M, Surana U. 2006. Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins. EMBO J. 25:2551–2563.
  • Cullen CF, Brittle AL, Ito T, Ohkura H. 2005. The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster. J. Cell Biol. 171:593–602.
  • Debec A, Marcaillou C. 1997. Structural alterations of the mitotic apparatus induced by the heat shock response in Drosophila cells. Biol. Cell 89:67–78.
  • Dobbelaere J, et al. 2008. A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PLoS Biol. 6:e224. https://doi.org/10.1371/journal.pbio.0060224.
  • Doxsey S, McCollum D, Theurkauf W. 2005. Centrosomes in cellular regulation. Annu. Rev. Cell Dev. Biol. 21:411–434.
  • Eliscovich C, Peset I, Vernos I, Mendez R. 2008. Spindle-localized CPE-mediated translation controls meiotic chromosome segregation. Nat. Cell Biol. 10:858–865.
  • Engler C, Gruetzner R, Kandzia R, Marillonnet S. 2009. Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes. PLoS One 4:e5553. https://doi.org/10.1371/journal.pone.0005553.
  • Engler C, Kandzia R, Marillonnet S. 2008. A one pot, one step, precision cloning method with high throughput capability. PLoS One 3:e3647. https://doi.org/10.1371/journal.pone.0003647.
  • Faust M, Montenarh M. 2000. Subcellular localization of protein kinase CK2. A key to its function? Cell Tissue Res. 301:329–340.
  • Giot L, et al. 2003. A protein interaction map of Drosophila melanogaster. Science 302:1727–1736.
  • Goldberg M, et al. 1998. Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA. Mol. Cell. Biol. 18:4315–4323.
  • Goshima G, et al. 2007. Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316:417–421.
  • Habedanck R, Stierhof YD, Wilkinson CJ, Nigg EA. 2005. The Polo kinase Plk4 functions in centriole duplication. Nat. Cell Biol. 7:1140–1146.
  • Hughes JR, et al. 2008. A microtubule interactome: complexes with roles in cell cycle and mitosis. PLoS Biol. 6:e98. https://doi.org/10.1371/journal.pbio.0060098.
  • Kalt A, Schliwa M. 1993. Molecular components of the centrosome. Trends Cell Biol. 3:118–128.
  • Keck JM, et al. 2011. A cell cycle phosphoproteome of the yeast centrosome. Science 332:1557–1561.
  • Keller LC, Romijn EP, Zamora I, Yates JRIII, Marshall WF. 2005. Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genes. Curr. Biol. 15:1090–1098.
  • Lane HA, Nigg EA. 1996. Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes. J. Cell Biol. 135:1701–1713.
  • Lange BM. 2002. Integration of the centrosome in cell cycle control, stress response and signal transduction pathways. Curr. Opin. Cell Biol. 14:35–43.
  • Lehmann V, Müller H, Lange BM. 2006. Immunoisolation of centrosomes from Drosophila melanogaster. Curr. Protoc. Cell Biol.3.17.1–3.17.13. https://doi.org/10.1002/0471143030.cb0317s29.
  • Li JB, et al. 2004. Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene. Cell 117:541–552.
  • Liska AJ, et al. 2004. Homology-based functional proteomics by mass spectrometry: application to the Xenopus microtubule-associated proteome. Proteomics 4:2707–2721.
  • Lowery DM, et al. 2007. Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate. EMBO J. 26:2262–2273.
  • Lucas EP, Raff JW. 2007. Maintaining the proper connection between the centrioles and the pericentriolar matrix requires Drosophila centrosomin. J. Cell Biol. 178:725–732.
  • Maiato H, et al. 2006. The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somatic cells. Chromosoma 115:469–480.
  • Malone CJ, et al. 2003. The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus. Cell 115:825–836.
  • Mirgorodskaya E, Braeuer C, Fucini P, Lehrach H, Gobom J. 2005. Nanoflow liquid chromatography coupled to matrix-assisted laser desorption/ionization mass spectrometry: sample preparation, data analysis, and application to the analysis of complex peptide mixtures. Proteomics 5:399–408.
  • Mishra RK, Chakraborty P, Arnaoutov A, Fontoura BM, Dasso M. 2010. The Nup107-160 complex and gamma-TuRC regulate microtubule polymerization at kinetochores. Nat. Cell Biol. 12:164–169.
  • Moritz M, Alberts BM. 1999. Isolation of centrosomes from Drosophila embryos. Methods Cell Biol. 61:1–12.
  • Müller H, Fogeron ML, Lehmann V, Lehrach H, Lange BM. 2006. A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint. Science 314:654–657.
  • Müller H, et al. 2010. Proteomic and functional analysis of the mitotic Drosophila centrosome. EMBO J. 29:3344–3357.
  • Nigg EA, Raff JW. 2009. Centrioles, centrosomes, and cilia in health and disease. Cell 139:663–678.
  • Nousiainen M, Sillje HH, Sauer G, Nigg EA, Korner R. 2006. Phosphoproteome analysis of the human mitotic spindle. Proc. Natl. Acad. Sci. U. S. A. 103:5391–5396.
  • Okuda M. 2002. The role of nucleophosmin in centrosome duplication. Oncogene 21:6170–6174.
  • Paddy MR, Saumweber H, Agard DA, Sedat JW. 1996. Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos. J. Cell Sci. 109:591–607.
  • Palazzo RE, Vogel JM, Schnackenberg BJ, Hull DR, Wu X. 2000. Centrosome maturation. Curr. Top. Dev. Biol. 49:449–470.
  • Portier N, et al. 2007. A microtubule-independent role for centrosomes and aurora a in nuclear envelope breakdown. Dev. Cell 12:515–529.
  • Qi H, et al. 2004. Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila. Mol. Biol. Cell 15:4854–4865.
  • Reineke U, Volkmer-Engert R, Schneider-Mergener J. 2001. Applications of peptide arrays prepared by the SPOT-technology. Curr. Opin. Biotechnol. 12:59–64.
  • Resendes KK, Rasala BA, Forbes DJ. 2008. Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export. Mol. Cell. Biol. 28:1755–1769.
  • Robinson JT, Wojcik EJ, Sanders MA, McGrail M, Hays TS. 1999. Cytoplasmic dynein is required for the nuclear attachment and migration of centrosomes during mitosis in Drosophila. J. Cell Biol. 146:597–608.
  • Salina D, et al. 2002. Cytoplasmic dynein as a facilitator of nuclear envelope breakdown. Cell 108:97–107.
  • Santamaria A, et al. 2011. The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol. Cell. Proteomics 10:M110.004457. https://doi.org/10.1074/mcp.M110.004457.
  • Sluder G. 2005. Two-way traffic: centrosomes and the cell cycle. Nat. Rev. Mol. Cell Biol. 6:743–748.
  • Somma MP, et al. 2008. Identification of Drosophila mitotic genes by combining co-expression analysis and RNA interference. PLoS Genet. 4:e1000126. https://doi.org/10.1371/journal.pgen.1000126.
  • Steen H, Mann M. 2004. The ABC's (and XYZ's) of peptide sequencing. Nat. Rev. Mol. Cell Biol. 5:699–711.
  • Sunkel CE, Glover DM. 1988. polo, a mitotic mutant of Drosophila displaying abnormal spindle poles. J. Cell Sci. 89:25–38.
  • Suprenant KA. 1993. Microtubules, ribosomes, and RNA: evidence for cytoplasmic localization and translational regulation. Cell Motil. Cytoskeleton 25:1–9.
  • Thingholm TE, Jorgensen TJ, Jensen ON, Larsen MR. 2006. Highly selective enrichment of phosphorylated peptides using titanium dioxide. Nat. Protoc. 1:1929–1935.
  • Tsai MY, et al. 2006. A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311:1887–1893.
  • Verollet C, et al. 2006. Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleation. J. Cell Biol. 172:517–528.
  • Wenschuh H, et al. 2000. Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides. Biopolymers 55:188–206.
  • Wigge PA, et al. 1998. Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J. Cell Biol. 141:967–977.
  • Zhai B, Villen J, Beausoleil SA, Mintseris J, Gygi SP. 2008. Phosphoproteome analysis of Drosophila melanogaster embryos. J. Proteome Res. 7:1675–1682.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.