The Motor Protein Myosin-X Transports VE-Cadherin along Filopodia To Allow the Formation of Early Endothelial Cell-Cell Contacts

REFERENCES

• Aberle, H., A. Bauer, J. Stappert, A. Kispert, and R. Kemler. 1997. Beta-catenin is a target for the ubiquitin-proteasome pathway. EMBO J. 16:3797–3804.
   PubMed Web of Science ®Google Scholar
• Adams, C. L., Y. T. Chen, S. J. Smith, and W. J. Nelson. 1998. Mechanisms of epithelial cell-cell adhesion and cell compaction revealed by high-resolution tracking of E-cadherin-green fluorescent protein. J. Cell Biol. 142:1105–1119.
   PubMed Web of Science ®Google Scholar
• Adams, C. L., W. J. Nelson, and S. J. Smith. 1996. Quantitative analysis of cadherin-catenin-actin reorganization during development of cell-cell adhesion. J. Cell Biol. 135:1899–1911.
   PubMed Web of Science ®Google Scholar
• Bear, J. E., and F. B. Gertler. 2009. Ena/VASP: towards resolving a pointed controversy at the barbed end. J. Cell Sci. 122:1947–1953.
   PubMed Web of Science ®Google Scholar
• Berg, J. S., and R. E. Cheney. 2002. Myosin-X is an unconventional myosin that undergoes intrafilopodial motility. Nat. Cell Biol. 4:246–250.
   PubMed Web of Science ®Google Scholar
• Berg, J. S., B. H. Derfler, C. M. Pennisi, D. P. Corey, and R. E. Cheney. 2000. Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin. J. Cell Sci. 113:3439–3451.
   PubMed Web of Science ®Google Scholar
• Block, J., T. E. Stradal, J. Hanisch, R. Geffers, S. A. Kostler, E. Urban, J. V. Small, K. Rottner, and J. Faix. 2008. Filopodia formation induced by active mDia2/Drf3. J. Microsc. 231:506–517.
   PubMedGoogle Scholar
• Bogatcheva, N. V., and A. D. Verin. 2008. The role of cytoskeleton in the regulation of vascular endothelial barrier function. Microvasc. Res. 76:202–207.
   PubMedGoogle Scholar
• Bohil, A. B., B. W. Robertson, and R. E. Cheney. 2006. Myosin-X is a molecular motor that functions in filopodia formation. Proc. Natl. Acad. Sci. U. S. A. 103:12411–12416.
   PubMed Web of Science ®Google Scholar
• Brawley, C. M., and R. S. Rock. 2009. Unconventional myosin traffic in cells reveals a selective actin cytoskeleton. Proc. Natl. Acad. Sci. U. S. A. 106:9685–9690.
   PubMedGoogle Scholar
• Breviario, F., L. Caveda, M. Corada, I. Martin-Padura, P. Navarro, J. Golay, M. Introna, D. Gulino, M. G. Lampugnani, and E. Dejana. 1995. Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin. Arterioscler. Thromb. Vasc. Biol. 15:1229–1239.
   PubMed Web of Science ®Google Scholar
• Campbell, R. E., O. Tour, A. E. Palmer, P. A. Steinbach, G. S. Baird, D. A. Zacharias, and R. Y. Tsien. 2002. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99:7877–7882.
   PubMed Web of Science ®Google Scholar
• Coue, M., S. L. Brenner, I. Spector, and E. D. Korn. 1987. Inhibition of actin polymerization by latrunculin A. FEBS Lett. 213:316–318.
   PubMed Web of Science ®Google Scholar
• Erdbruegger, U., M. Haubitz, and A. Woywodt. 2006. Circulating endothelial cells: a novel marker of endothelial damage. Clin. Chim. Acta 373:17–26.
   PubMed Web of Science ®Google Scholar
• Faix, J., D. Breitsprecher, T. E. Stradal, and K. Rottner. 2009. Filopodia: complex models for simple rods. Int. J. Biochem. Cell Biol. 41:1656–1664.
   PubMed Web of Science ®Google Scholar
• Gulino, D., E. Delachanal, E. Concord, Y. Genoux, B. Morand, M. O. Valiron, E. Sulpice, R. Scaife, M. Alemany, and T. Vernet. 1998. Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin. J. Biol. Chem. 273:29786–29793.
   PubMedGoogle Scholar
• Gupton, S. L., and F. B. Gertler. 2007. Filopodia: the fingers that do the walking. Sci. STKE 2007:re5.
   PubMedGoogle Scholar
• Hermant, B., S. Bibert, E. Concord, B. Dublet, M. Weidenhaupt, T. Vernet, and D. Gulino-Debrac. 2003. Identification of proteases involved in the proteolysis of vascular endothelium cadherin during neutrophil transmigration. J. Biol. Chem. 278:14002–14012.
   PubMed Web of Science ®Google Scholar
• Heyraud, S., M. Jaquinod, C. Durmort, E. Dambroise, E. Concord, J. P. Schaal, P. Huber, and D. Gulino-Debrac. 2008. Contribution of annexin 2 to the architecture of mature endothelial adherens junctions. Mol. Cell. Biol. 28:1657–1668.
   PubMedGoogle Scholar
• Homma, K., J. Saito, R. Ikebe, and M. Ikebe. 2001. Motor function and regulation of myosin X. J. Biol. Chem. 276:34348–34354.
   PubMedGoogle Scholar
• Kerber, M. L., D. T. Jacobs, L. Campagnola, B. D. Dunn, T. Yin, A. D. Sousa, O. A. Quintero, and R. E. Cheney. 2009. A novel form of motility in filopodia revealed by imaging myosin-X at the single-molecule level. Curr. Biol. 19:967–973.
   PubMed Web of Science ®Google Scholar
• Lampugnani, M. G., M. Resnati, M. Raiteri, R. Pigott, A. Pisacane, G. Houen, L. P. Ruco, and E. Dejana. 1992. A novel endothelial-specific membrane protein is a marker of cell-cell contacts. J. Cell Biol. 118:1511–1522.
   PubMed Web of Science ®Google Scholar
• Maddugoda, M. P., M. S. Crampton, A. M. Shewan, and A. S. Yap. 2007. Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells. J. Cell Biol. 178:529–540.
   PubMed Web of Science ®Google Scholar
• Mallavarapu, A., and T. Mitchison. 1999. Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction. J. Cell Biol. 146:1097–1106.
   PubMed Web of Science ®Google Scholar
• Martel, V., L. Vignoud, S. Dupe, P. Frachet, M. R. Block, and C. Albiges-Rizo. 2000. Talin controls the exit of the integrin alpha 5 beta 1 from an early compartment of the secretory pathway. J. Cell Sci. 113:1951–1961.
   PubMed Web of Science ®Google Scholar
• Mattila, P. K., and P. Lappalainen. 2008. Filopodia: molecular architecture and cellular functions. Nat. Rev. Mol. Cell Biol. 9:446–454.
   PubMed Web of Science ®Google Scholar
• Mege, R. M., J. Gavard, and M. Lambert. 2006. Regulation of cell-cell junctions by the cytoskeleton. Curr. Opin. Cell Biol. 18:541–548.
   PubMed Web of Science ®Google Scholar
• Pi, X., R. Ren, R. Kelley, C. Zhang, M. Moser, A. B. Bohil, M. Divito, R. E. Cheney, and C. Patterson. 2007. Sequential roles for myosin-X in BMP6-dependent filopodial extension, migration, and activation of BMP receptors. J. Cell Biol. 179:1569–1582.
   PubMed Web of Science ®Google Scholar
• Pollard, T. D. 2007. Regulation of actin filament assembly by Arp2/3 complex and formins. Annu. Rev. Biophys. Biomol. Struct. 36:451–477.
   PubMedGoogle Scholar
• Raich, W. B., C. Agbunag, and J. Hardin. 1999. Rapid epithelial-sheet sealing in the Caenorhabditis elegans embryo requires cadherin-dependent filopodial priming. Curr. Biol. 9:1139–1146.
   PubMedGoogle Scholar
• Rudini, N., and E. Dejana. 2008. Adherens junctions. Curr. Biol. 18:R1080–R1082.
   PubMed Web of Science ®Google Scholar
• Schelhaas, M., H. Ewers, M. L. Rajamaki, P. M. Day, J. T. Schiller, and A. Helenius. 2008. Human papillomavirus type 16 entry: retrograde cell surface transport along actin-rich protrusions. PLoS Pathog. 4:e1000148.
   PubMed Web of Science ®Google Scholar
• Sousa, A. D., J. S. Berg, B. W. Robertson, R. B. Meeker, and R. E. Cheney. 2006. Myo10 in brain: developmental regulation, identification of a headless isoform and dynamics in neurons. J. Cell Sci. 119:184–194.
   PubMed Web of Science ®Google Scholar
• Svitkina, T. M., E. A. Bulanova, O. Y. Chaga, D. M. Vignjevic, S. Kojima, J. M. Vasiliev, and G. G. Borisy. 2003. Mechanism of filopodia initiation by reorganization of a dendritic network. J. Cell Biol. 160:409–421.
   PubMed Web of Science ®Google Scholar
• Tesfamariam, B., and A. F. DeFelice. 2007. Endothelial injury in the initiation and progression of vascular disorders. Vascul. Pharmacol. 46:229–237.
   PubMed Web of Science ®Google Scholar
• Tokuo, H., and M. Ikebe. 2004. Myosin X transports Mena/VASP to the tip of filopodia. Biochem. Biophys. Res. Commun. 319:214–220.
   PubMed Web of Science ®Google Scholar
• Tokuo, H., K. Mabuchi, and M. Ikebe. 2007. The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation. J. Cell Biol. 179:229–238.
   PubMed Web of Science ®Google Scholar
• Vasioukhin, V., C. Bauer, M. Yin, and E. Fuchs. 2000. Directed actin polymerization is the driving force for epithelial cell-cell adhesion. Cell 100:209–219.
   PubMed Web of Science ®Google Scholar
• Vasioukhin, V., and E. Fuchs. 2001. Actin dynamics and cell-cell adhesion in epithelia. Curr. Opin. Cell Biol. 13:76–84.
   PubMed Web of Science ®Google Scholar
• Wallez, Y., F. Cand, F. Cruzalegui, C. Wernstedt, S. Souchelnytskyi, I. Vilgrain, and P. Huber. 2007. Src kinase phosphorylates vascular endothelial-cadherin in response to vascular endothelial growth factor: identification of tyrosine 685 as the unique target site. Oncogene 26:1067–1077.
   PubMed Web of Science ®Google Scholar
• Waschke, J., F. E. Curry, R. H. Adamson, and D. Drenckhahn. 2005. Regulation of actin dynamics is critical for endothelial barrier functions. Am. J. Physiol. Heart Circ. Physiol. 288:H1296–H1305.
   PubMed Web of Science ®Google Scholar
• Wood, W., and P. Martin. 2002. Structures in focus—filopodia. Int. J. Biochem. Cell Biol. 34:726–730.
   PubMed Web of Science ®Google Scholar
• Zhang, H., J. S. Berg, Z. Li, Y. Wang, P. Lang, A. D. Sousa, A. Bhaskar, R. E. Cheney, and S. Stromblad. 2004. Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. 6:523–531.
   PubMed Web of Science ®Google Scholar