The Bulk Chromatin Structure of a Murine Transgene Does Not Vary with Its Transcriptional or DNA Methylation Status

REFERENCES

• Antequera, F., D. Macleod, and A. P. Bird. 1989. Specific protection of methylated CpGs in mammalian nuclei. Cell 58:509–517.
   PubMedGoogle Scholar
• Ashburner, M., C. Chihara, P. Meltzer, and G. Richards. 1974. Temporal control of puffing activity in polytene chromosomes. Cold Spring Harbor Symp. Quant. Biol. 38:655–662.
   PubMedGoogle Scholar
• Bartolomei, M. S., A. L. Webber, M. E. Brunkow, and S. M. Tilghman. 1993. Epigenetic mechanisms underlying the imprinting of the mouse H19 gene. Genes Dev. 7:1663–1673.
   PubMed Web of Science ®Google Scholar
• Bestor, T. H., S. B. Hellewell, and V. M. Ingram. 1984. Differentiation of two mouse cell lines is associated with hypomethylation of their genomes. Mol. Cell. Biol. 4:1800–1806.
   PubMedGoogle Scholar
• Bestor, T. H., and V. M. Ingram. 1983. Two DNA methyltransferases from murine erythroleukemia cells: purification, sequence specificity, and mode of interaction with DNA. Proc. Natl. Acad. Sci. USA 80:5559–5563.
   PubMed Web of Science ®Google Scholar
• Boyes, J., and A. Bird. 1991. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein. Cell 64:1123–1134.
   PubMed Web of Science ®Google Scholar
• Buschhausen, G., B. Wittig, M. Graessmann, and A. Graessmann. 1987. Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene. Proc. Natl. Acad. Sci. USA 84: 1177–1181.
   PubMedGoogle Scholar
• Butkus, V., L. Petrauskiene, Z. Maneliene, S. Klimasauskas, V. Laucys, and A. Janulaitis. 1987. Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr91 restriction endonucleases. Nucleic Acids Res. 15:7091–7102.
   PubMedGoogle Scholar
• Cedar, H. 1988. DNA methylation and gene activity. Cell 53:3–4.
   PubMed Web of Science ®Google Scholar
• Crouse, G., C. Simonsen, R. McEwan, and R. Schimke. 1982. Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells. J. Biol. Chem. 257:7887–7897.
   PubMedGoogle Scholar
• Durnam, D. D., and R. D. Palmiter. 1981. Transcriptional regulation of the mouse metallothionein-I gene by heavy metals. J. Biol. Chem. 256:5712–5716.
   PubMed Web of Science ®Google Scholar
• Durrin, L. K., J. L. Weber, and J. Gorski. 1984. Chromatin structure, transcription, and methylation of the prolactin gene domain in pituitary tumors of Fischer 344 rats. J. Biol. Chem. 259:7086–7093.
   PubMedGoogle Scholar
• Engler, P., D. Haasch, C. A. Pinkert, L. Doglio, M. Glymour, R. Brinster, and U. Storb. 1991. A strain-specific modifier on mouse chromosome 4 controls the methylation of independent transgene loci. Cell 65:939–947.
   PubMedGoogle Scholar
• Engler, P., and U. Storb. 1987. High-frequency deletional rearrangement of immunoglobulin k gene segments introduced into a pre-B-cell line. Proc. Natl. Acad. Sci. USA 84:4949–4953.
   PubMedGoogle Scholar
• Engler, P., A. Weng, and U. Storb. 1993. Influence of CpG methylation and target spacing on V(D)J recombination in a transgenic substrate. Mol. Cell. Biol. 13:571–577.
   PubMedGoogle Scholar
• Gilman, M. 1993. Ribonuclease protection assay, p. 4.7.1–4.7.8. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology. John Wiley & Sons, Inc., New York.
   Google Scholar
• Groudine, M., R. Eisenman, and H. Weintraub. 1981. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA meth-ylation. Nature (London) 292:311–317.
   PubMed Web of Science ®Google Scholar
• Hitt, M. M., T.-L. Wu, G. Cohen, and S. Linn. 1988. De novo and maintenance DNA methylation by a mouse plasmacytoma cell DNA methyltrans-ferase. J. Biol. Chem. 263:4392–4399.
   PubMedGoogle Scholar
• Holler, M., G. Westin, J. Jiricny, and W. Schaffner. 1988. Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated. Genes Dev. 2:1127–1135.
   PubMed Web of Science ®Google Scholar
• Hsieh, C.-L., and M. R. Lieber. 1992. CpG methylated minichromosomes become inaccessible for V(D)J recombination after undergoing replication. EMBO J. 11:315–325.
   PubMed Web of Science ®Google Scholar
• Jenuwein, T., W. Forrester, R. Qiu, and R. Grosschedl. 1993. The immuno-globulin m enhancer core establishes local factor access in nuclear chromatin independent of transcriptional stimulation. Genes Dev. 7:2016–2032.
   PubMedGoogle Scholar
• Jones, P. 1985. Altering gene expression with 5-azacytidine. Cell 40:485–486.
   PubMed Web of Science ®Google Scholar
• Kass, S. U., J. P. Goddard, and R. L. P. Adams. 1993. Inactive chromatin spreads from a focus of methylation. Mol. Cell. Biol. 13:7372–7379.
   PubMedGoogle Scholar
• Lamb, M. M., and B. Daneholt. 1979. Characterization of active transcription units in Balbani rings of Chironomus tentans. Cell 17:835–848.
   PubMed Web of Science ®Google Scholar
• Lewis, J. D., R. R. Meehan, W. J. Henzel, I. Maurer-Fogy, P. Jeppesen, F. Klein, and A. Bird. 1992. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell 69:905–914.
   PubMed Web of Science ®Google Scholar
• Li, E., T. Bestor, and R. Jaenisch. 1992. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69:915–926.
   PubMed Web of Science ®Google Scholar
• MacDonald, R. J., G. H. Swift, A. E. Przybyla, and J. M. Chirgwin. 1987. Isolation of RNA using guanidinium salts. Methods Enzymol. 152:219–227.
   PubMed Web of Science ®Google Scholar
• Marzluff, W. F., and R. C. C. Huang. 1984. Transcription of RNA in isolated nuclei, p. 89–95. In B. D. Hames, and S. J. Higgins (ed.), In vitro transcription and translation—a practical approach. IRL Press, Washington, D.C.
   Google Scholar
• Mather, E. L., and R. P. Perry. 1983. Methylation status and DNaseI sensitivity of immunoglobulin genes: changes associated with rearrangement. Proc. Natl. Acad. Sci. USA 80:4689–4693.
   PubMed Web of Science ®Google Scholar
• Meehan, R. R., J. D. Lewis, S. McKay, E. L. Kleiner, and A. P. Bird. 1989. Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs. Cell 58:499–507.
   PubMed Web of Science ®Google Scholar
• Molloy, P. L., and F. Watt. 1990. DNA methylation and specific protein-DNA interactions. Philos. Trans. R. Soc. Lond. B 326:267–275.
   PubMedGoogle Scholar
• Palmiter, R. (University of Washington). 1994. Personal communication.
   Google Scholar
• Pfeifer, G. P., and A. D. Riggs. 1991. Chromatin differences between active and inactive X chromosomes revealed by genomic footprinting of permeabi-lized cells using DNase I and ligation-mediated PCR. Genes Dev. 5:1102–1113.
   PubMedGoogle Scholar
• Reitman, M., E. Lee, H. Westphal, and G. Felsenfeld. 1993. An enhancer/ locus control region is not sufficient to open chromatin. Mol. Cell. Biol. 13: 3990–3998.
   PubMedGoogle Scholar
• Roth, P. E., L. Doglio, J. T. Manz, J. Y. Kim, D. Lo, and U. Storb. 1993. Immunoglobulin γ2b transgenes inhibit heavy chain gene rearrangement, but cannot promote B cell development. J. Exp. Med. 178:2007–2021.
   PubMedGoogle Scholar
• Rudin, C. M., P. Engler, and U. Storb. 1990. Differential splicing of thymosin β4 mRNA. J. Immunol. 144:4857–4862.
   PubMed Web of Science ®Google Scholar
• Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Storb, U., and B. Arp. 1983. Methylation patterns of immunoglobulin genes in lymphoid cells: correlation of expression and differentiation with under-methylation. Proc. Natl. Acad. Sci. USA 80:6642–6646.
   PubMed Web of Science ®Google Scholar
• Stuart, G. W., P. F. Searle, H. Y. Chen, R. L. Brinster, and R. D. Palmiter. 1984. A 12-base-pair DNA motif that is repeated several times in metallo-thionein gene promoters confers metal regulation to a heterologous gene. Proc. Natl. Acad. Sci. USA 81:7318–7322.
   PubMed Web of Science ®Google Scholar
• Tratner, I., J. L. Nahon, J. M. Sala-Trepat, and A. Venetianer. 1987. Albumin and alpha-fetoprotein gene transcription in rat hepatoma cell lines is correlated with specific DNA hypomethylation and altered chromatin structure in the 59 region. Mol. Cell. Biol. 7:1856–1864.
   PubMedGoogle Scholar
• Watt, F., and P. L. Molloy. 1988. Cytosine methylation prevents binding to DNA of a HeLa cell transcription factor required for optimal expression of the adenovirus major late promoter. Genes Dev. 2:1136–1143.
   PubMed Web of Science ®Google Scholar
• Weih, F., D. Nitsch, A. Reik, G. Schütz, and P. B. Becker. 1991. Analysis of CpG methylation and genomic footprinting at the tyrosine aminotransferase gene: DNA methylation alone is not sufficient to prevent protein binding in vivo. EMBO J. 10:2559–2567.
   PubMedGoogle Scholar
• Weng, A., T. Magnuson, and U. Storb. Submitted for publication.
   Google Scholar