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Research Article

Presence of Exon Splicing Silencers within Human Immunodeficiency Virus Type 1 tat Exon 2 and tat-rev Exon 3: Evidence for Inhibition Mediated by Cellular Factors

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Pages 4606-4615 | Received 28 Nov 1994, Accepted 17 Apr 1995, Published online: 30 Mar 2023

REFERENCES

  • Adachi, A., H. E. Gendelman, S. Koenig, T. Folks, R. Willey, A. Rabson, and M. A. Martin. 1986. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J. Virol. 59:284–291.
  • Amendt, B. A., D. Hesslein, L.-J. Chang, and C. M. Stoltzfus. 1994. Presence of negative and positive cis-acting RNA splicing elements within and flanking the first tat coding exon of human immunodeficiency virus type 1. Mol. Cell. Biol. 14:3960–3970.
  • Amendt, B. A., and C. M. Stoltzfus. Unpublished data.
  • Black, D. L. 1992. Activation of c-src neuron-specific splicing by an unusual RNA element in vivo and in vitro. Cell 69:795–807.
  • Caputi, M., G. Casari, S. Guenzi, R. Tagliabue, A. Sidoli, C. A. Melo, and F. E. Baralle. 1994. A novel bipartite splicing enhancer modulates the differential processing of the human fibronectin EDA exon. Nucleic Acids Res. 22:1018–1022.
  • Cote, G. J., D. T. Stolow, S. Peleg, S. M. Berget, and R. F. Gagel. 1992. Identification of exon sequences and an exon binding protein involved in alternative RNA splicing of calcitonin/CGRP. Nucleic Acids Res. 20:2361–2366.
  • Cullen, B. R. 1991. Regulation of human immunodeficiency virus replication. Annu. Rev. Microbiol. 45:219–250.
  • Delsert, C., N. Morin, and D. F. Klessig. 1989. cis-acting elements and a trans-acting factor affecting alternative splicing of adenovirus L1 transcripts. Mol. Cell. Biol. 9:4364–4371.
  • Dirksen, W. P., R. K. Hampson, Q. Sun, and F. M. Rottman. 1994. A purine-rich exon sequence enhances alternative splicing of bovine growth hormone pre-mRNA. J. Biol. Chem. 269:6431–6436.
  • Emerman, M., R. Vazeux, and K. Peden. 1989. The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization. Cell 57:1155–1165.
  • Emeson, R. B., F. Hedjran, J. M. Yeakley, J. W. Guise, and M. G. Rosenfeld. 1989. Alternative production of calcitonin and CGRP mRNA is regulated at the calcitonin-specific splice acceptor. Nature (London) 341:76–80.
  • Felber, B. K., C. M. Drysdale, and G. N. Pavlakis. 1990. Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein. J. Virol. 64:3734–3741.
  • Felber, B. K., M. Hadzopoulou-Cladaras, C. Cladaras, T. Copeland, and G. N. Pavlakis. 1989. Rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA. Proc. Natl. Acad. Sci. USA 86:1495–1499.
  • Fischer, U., S. Meyer, M. Teufel, C. Heckel, R. Luhrmann, and G. Rautmann. 1994. Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA. EMBO J. 13:4105–4112.
  • Fu, X.-D. 1993. Specific commitment of different pre-mRNAs to splicing by single SR proteins. Nature (London) 365:82–85.
  • Fu, X.-D., R. A. Katz, A. M. Skalka, and T. Maniatis. 1991. The role of branchpoint and 39-exon sequences in the control of balanced splicing of avian retrovirus RNA. Genes Dev. 5:211–220.
  • Gallego, M. E., L. Balvay, and E. Brody. 1992. cis-acting sequences involved in exon selection in the chicken β-tropomyosin gene. Mol. Cell. Biol. 12: 5415–5425.
  • Ge, H., and J. L. Manley. 1990. A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell 62:25–34.
  • Graham, I. R., M. Hamshere, and I. C. Eperon. 1992. Alternative splicing of a human α-tropomyosin muscle-specific exon: identification of determining sequences. Mol. Cell. Biol. 12:3872–3882.
  • Green, M. R. 1991. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing. Annu. Rev. Cell Biol. 7:559–599.
  • Guatelli, J. C., T. R. Gingeras, and D. D. Richman. 1990. Alternative splice acceptor utilization during human immunodeficiency virus type 1 infection of cultured cells. J. Virol. 64:4093–4098.
  • Guo, W., G. J. Mulligan, S. Wormsley, and D. M. Helfman. 1991. Alternative splicing of beta-tropomyosin pre-mRNA: cis-acting elements and cellular factors that block the use of a skeletal muscle exon in nonmuscle cells. Genes Dev. 5:2096–2107.
  • Hammarskjöld, M.-L., J. Heimer, B. Hammarskjöld, I. Sangwan, L. Albert, and D. Rekosh. 1989. Regulation of human immunodeficiency virus env expression by the rev gene product. J. Virol. 63:1959–1966.
  • Hedley, M. L., and T. Maniatis. 1991. Sex-specific splicing and polyadenylation of dsx pre-mRNA requires a sequence that binds specifically to tra-2 protein in vitro. Cell 65:579–586.
  • Hoffman, B. E., and P. J. Grabowski. 1992. U1 snRNP targets an essential splicing factor, U2AF65, to the 39 splice site by a network of interactions spanning the exon. Genes Dev. 6:2554–2568.
  • Horabin, J. I., and P. Schedl. 1993. Sex-lethal autoregulation requires multiple cis-acting elements upstream and downstream of the male exon and appears to depend largely on controlling the use of the male exon 59 splice site. Mol. Cell. Biol. 13:7734–7746.
  • Katz, R. A., and A. M. Skalka. 1990. Control of retroviral RNA splicing through maintenance of suboptimal processing signals. Mol. Cell. Biol. 10: 696–704.
  • Konarska, M. M. 1989. Analysis of splicing complexes and small ribonucle-oprotein particles by native gel electrophoresis. Methods Enzymol. 180:442–453.
  • Krainer, A. R., G. C. Conway, and D. Kozak. 1990. The essential pre-mRNA splicing factor SF2 influences 59 splice site selection by activating proximal sites. Cell 62:35–42.
  • Krainer, A. R., G. C. Conway, and D. Kozak. 1990. Purification and charac-terization of pre-mRNA splicing factor SF2 from HeLa cells. Genes Dev. 4:1158–1171.
  • Laviguer, A., H. La Branche, A. R. Kornblihtt, and B. Chabot. 1993. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding. Genes Dev. 7:2405–2417.
  • Malim, M. H., and B. R. Cullen. 1993. Rev and the fate of pre-mRNA in the nucleus: implications for the regulation of RNA processing in eukaryotes. Mol. Cell. Biol. 13:6180–6189.
  • Malim, M. H., J. Hauber, S.-Y. Le, J. V. Maizel, and B. R. Cullen. 1989. The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA. Nature (London) 338:254–257.
  • Mayeda, A., D. M. Helfman, and A. R. Krainer. 1993. Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein A1 and pre-mRNA splicing factor SF2/ASF. Mol. Cell. Biol. 13:2993–3001.
  • Muesing, M. A., D. H. Smith, C. D. Cabradilla, C. V. Benton, L. A. Lasky, and D. J. Capon. 1985. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus. Nature (London) 313:450–458.
  • Mulligan, G. J., W. Guo, S. Wormsley, and D. M. Helfman. 1992. Polypyri-midine tract binding protein interacts with sequences involved in alternative splicing of beta-tropomyosin pre-mRNA. J. Biol. Chem. 267:25480–25487.
  • Nagoshi, R. N., and B. S. Baker. 1990. Regulation of sex-specific RNA splicing at the Drosophila doublesex gene: cis-acting mutations in exon sequence alter sex-specific RNA splicing patterns. Genes Dev. 4:89–97.
  • Purcell, D. F. J., and M. A. Martin. 1993. Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity. J. Virol. 67:6365–6378.
  • Robberson, B. L., G. J. Cote, and S. M. Berget. 1990. Exon definition may facilitate splice site selection in RNAs with multiple exons. Mol. Cell. Biol. 10:84–94.
  • Robert-Guroff, M., M. Popovic, S. Gartner, P. Markham, R. C. Gallo, and M. S. Reitz. 1990. Structure and expression of tat-, rev-, and nef-specific transcripts of human immunodeficiency virus type 1 in infected lymphocytes and macrophages. J. Virol. 64:3391–3398.
  • Roesser, J. R., K. Liittschwager, and S. E. Leff. 1993. Regulation of tissue-specific splicing of the calcitonin/calcitonin gene-related peptide gene by RNA-binding proteins. J. Biol. Chem. 268:8366–8375.
  • Ruskin, B., A. R. Krainer, T. Maniatis, and M. R. Green. 1984. Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro. Cell 38:317–331.
  • Sadaie, M. R., J. Rappaport, T. Benter, S. F. Josephs, R. Willis, and F. Wong-Staal. 1988. Missense mutations in an infectious human immunodeficiency viral genome: functional mapping of tat and identification of the rev splice acceptor. Proc. Natl. Acad. Sci. USA 85:9224–9228.
  • Sakamoto, H., K. Inoue, I. Higuchi, Y. Ono, and Y. Shimura. 1992. Control of Drosophila Sex-lethal pre-mRNA splicing by its own female-specific product. Nucleic Acids Res. 20:5533–5540.
  • Schwartz, S., B. K. Felber, D. M. Benko, E.-M. Fenyö, and G. N. Pavlakis. 1990. Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1. J. Virol. 64:2519–2529.
  • Siebel, C. W., L. D. Fresco, and D. C. Rio. 1992. The mechanism of somatic inhibition of Drosophila P element pre-mRNA splicing: multiprotein complexes at an exon pseudo-59 splice site control U1 snRNA binding. Genes Dev. 6:1386–1401.
  • Siebel, C. W., R. Kanaar, and D. C. Rio. 1994. Regulation of tissue-specific P-element pre-mRNA splicing requires the RNA-binding protein PSI. Genes Dev. 8:1713–1725.
  • Staffa, A., and A. Cochrane. 1994. The tat/rev intron of human immunodeficiency virus type 1 is inefficiently spliced because of suboptimal signals in the 39 splice site. J. Virol. 68:3071–3079.
  • Staffa, A., and A. Cochrane. 1995. Identification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1. Mol. Cell. Biol. 15:4597–4605.
  • Staknis, D., and R. Reed. 1994. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex. Mol. Cell. Biol. 14:7670–7682.
  • Sun, Q., A. Mayeda, R. K. Hampson, A. R. Krainer, and F. M. Rottman. 1993. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes Dev. 7:2598–2608.
  • Tanaka, K., A. Watakabe, and Y. Shimura. 1994. Polypurine sequences within a downstream exon function as a splicing enhancer. Mol. Cell. Biol. 14:1347–1354.
  • Tian, M., and T. Maniatis. 1992. Positive control of pre-mRNA splicing. Science 256:237–240.
  • Tian, M., and T. Maniatis. 1993. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA. Cell 74:105–114.
  • Tian, M., and T. Maniatis. 1994. A splicing enhancer exhibits both constitutive and regulated activities. Genes Dev. 8:1703–1712.
  • Tsai, A. Y. M., M. Streuli, and H. Saito. 1989. Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA. Mol. Cell. Biol. 9:4550–4555.
  • van Oers, C. C. M., G. J. Adema, H. Zandberg, T. C. Moen, and P. D. Baas. 1994. Two different sequence elements within exon 4 are necessary for calcitonin-specific splicing of the human calcitonin/calcitonin gene-related peptide I pre-mRNA. Mol. Cell. Biol. 14:951–960.
  • Vilardell, J., and J. R. Warner. 1994. Regulation of splicing at an intermediate step in the formation of the spliceosome. Genes Dev. 8:211–220.
  • Watakabe, A., K. Tanaka, and Y. Shimura. 1993. The role of exon sequences in splice site selection. Genes Dev. 7:407–418.
  • Xu, R., J. Teng, and T. A. Cooper. 1993. The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element. Mol. Cell. Biol. 13:3660–3674.
  • Yeakley, J. M., F. Hedjran, J.-P. Morfin, N. Merillat, M. G. Rosenfeld, and R. B. Emeson. 1993. Control of calcitonin/calcitonin gene-related peptide pre-mRNA processing by constitutive intron and exon elements. Mol. Cell. Biol. 13:5999–6011.
  • Zahler, A. M., K. M. Neugebauer, W. S. Lane, and M. B. Roth. 1993. Distinct functions of SR proteins in alternative pre-mRNA splicing. Science 260:219–222.
  • Zamore, P. D., and M. R. Green. 1989. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. Proc. Natl. Acad. Sci. USA 86:9243–9247.
  • Zamore, P. D., and M. R. Green. 1992. Cloning and domain structure of the mammalian splicing factor U2AF. Nature (London) 355:609–614.
  • Zhang, L., and C. M. Stoltzfus. 1995. A suboptimal src 39 splice site is necessary for efficient replication of Rous sarcoma virus. Virology 206:1099–1107.
  • Zhao, Q., R. V. Schoborg, and D. J. Pintel. 1994. Alternative splicing of pre-mRNAs encoding the nonstructural proteins of minute virus of mice is facilitated by sequences within the downstream intron. J. Virol. 68:2849–2859.

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