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Research Article

Protein-DNA Interactions at the Major and Minor Promoters of the Divergently Transcribed dhfr and rep3 Genes during the Chinese Hamster Ovary Cell Cycle

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Pages 634-647 | Received 19 Sep 1995, Accepted 15 Nov 1995, Published online: 29 Mar 2023

REFERENCES

  • Araki, E., T. Murakami, T. Shirotani, F. Kanai, Y. Shinohara, F. Shimada, M. Mori, M. Shichiri, and Y. Ebina. 1991. A cluster of four Sp1 binding sites required for efficient expression of the human insulin gene. J. Biol. Chem. 266:3944–3948.
  • Azizkhan, J. C., D. E. Jensen, A. J. Pierce, and M. Wade. 1993. Transcription from TATA-less promoters: dihydrofolate reductase as a model. Crit. Rev. Eukaryotic Gene Expr. 3:229–254.
  • Azizkhan, J. C., J. P. Vaughn, R. J. Christy, and J. L. Hamlin. 1986. Nucleotide sequence and nuclease hypersensitivity of the Chinese hamster dihydrofolate reductase gene promoter region. Biochemistry 25:6228–6236.
  • Bandara, L. R., V. M. Buck, M. Zamanian, L. H. Johnston, and N. B. La Thangue. 1993. Functional synergy between DP-1 and E2F-1 in the cell cycle-regulating transcription factor DRTF1/E2F. EMBO J. 12:4317–4324.
  • Beijersbergen, R. L., R. M. Kerkhoven, L. Zhu, L. Carlee, P. M. Voorhoeve, and R. Bernards. 1994. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo. Genes Dev. 8:2680–2690.
  • Blake, M. C., and J. C. Azizkhan. 1989. Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo. Mol. Cell. Biol. 9:4994–5002.
  • Blake, M. C., R. C. Jambou, A. G. Swick, J. W. Kahn, and J. C. Azizkhan. 1990. Transcriptional initiation is controlled by upstream GC-box interactions in a TATAA-less promoter. Mol. Cell. Biol. 10:6632–6641.
  • Cao, L., B. Faha, M. Dembski, L.-H. Tsai, E. Harlow, and N. Dyson. 1992. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F. Nature (London) 355:176–179.
  • Chellappan, S. P., S. Hiebert, M. Mudryj, J. M. Horowitz, and J. R. Nevins. 1991. The E2F transcription factor is a cellular target for the RB protein. Cell 65:1053–1061.
  • Chen, J., and D. S. Pederson. 1993. A distal heat shock element promotes the rapid response to heat shock of the HSP26 gene in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 268:7442–7448.
  • Chen, X., J. C. Azizkhan, and D. C. Lee. 1992. The binding of transcription factor Sp1 to multiple sites is required for maximal expression from the rat transforming growth factor alpha promoter. Oncogene 7:1805–1815.
  • Ciudad, C. J., A. E. Morris, C. Jeng, and L. A. Chasin. 1992. Point mutational analysis of the hamster dihydrofolate reductase minimum promoter. J. Biol. Chem. 267:3650–3656.
  • Cobrinik, D., P. Whyte, D. S. Peeper, T. Jacks, and R. A. Weinberg. 1993. Cell cycle-specific association of E2F with the p130 E1A-binding protein. Genes Dev. 7:2392–2404.
  • Crouse, G. F., E. J. Leys, R. N. McEwan, E. G. Frayne, and R. E. Kellems. 1985. Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene. Mol. Cell. Biol. 5:1847–1858.
  • DeGregori, J., T. Kowalik, and J. R. Nevins. 1995. Cellular targets for activation by the E2F1 transcription factor include DNA synthesis- and G1/S-regulatory genes. Mol. Cell. Biol. 15:4215–4224.
  • Devoto, S. H., M. Mudryj, J. Pines, T. Hunter, and J. R. Nevins. 1992. A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity: p33cdk2 is a component of the E2F-cyclin A complex. Cell 68: 167–176.
  • Diffley, J. F. X., J. H. Cocker, S. J. Dowell, and A. Rowley. 1994. Two steps in the assembly of complexes at yeast replication origins in vivo. Cell 78: 303–316.
  • Dijkwel, P. A., and J. L. Hamlin. 1992. Initiation of DNA replication in the dihydrofolate reductase locus is confined to the early S period in CHO cells synchronized with the plant amino acid mimosine. Mol. Cell. Biol. 12:3715–3722.
  • Dynlacht, B. D., O. Flores, J. A. Lees, and E. Harlow. 1994. Differential regulation of E2F trans-activation by cyclin/cdk2 complexes. Genes Dev. 8: 1772–1786.
  • Dyson, N., M. Dembski, A. Fattaey, C. Ngwu, M. Ewen, and K. Helin. 1993. Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1. J. Virol. 67:7641–7647.
  • Eastman, H. B., A. G. Swick, M. C. Schmitt, and J. C. Azizkhan. 1991. Stimulation of dihydrofolate reductase promoter activity by antimetabolic drugs. Proc. Natl. Acad. Sci. USA 88:8572–8576.
  • Farnham, P. J., and A. L. Means. 1990. Sequences downstream of the transcription initiation site modulate the activity of the murine dihydrofolate reductase promoter. Mol. Cell. Biol. 10:1390–1398.
  • Farnham, P. J., and R. T. Schimke. 1985. Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle. Mol. Cell. Biol. 6:365–371.
  • Farnham, P. J., and R. T. Schimke. 1986. Murine dihydrofolate reductase transcripts through the cell cycle. Mol. Cell. Biol. 6:2392–2401.
  • Farnham, P. J., and R. T. Schimke. 1986. In vitro transcription and delimitation of promoter elements of the murine dihydrofolate reductase gene. Mol. Cell. Biol. 6:2392–2401.
  • Farnham, P. J., J. E. Slansky, and R. Kollmar. 1993. The role of E2F in the mammalian cell cycle. Biochim. Biophys. Acta 1155:125–131.
  • Ginsberg, D., G. Vairo, T. Chittenden, Z.-X. Xiao, G. Xu, K. L. Wydner, J. A. DeCaprio, J. B. Lawrence, and D. M. Livingston. 1994. E2F-4, a new member of the E2F transcription factor family, interacts with p107. Genes Dev. 8: 2665–2679.
  • Girling, R., L. R. Bandara, E. Ormondroyd, E. W.-F. Lam, S. Kotecha, T. Mohun, and N. B. La Thangue. Molecular characterization of Xenopus laevis DP proteins. Mol. Biol. Cell 5:1081–1092.
  • Girling, R., J. F. Partridge, L. R. Bandara, N. Burden, N. F. Totty, J. J. Hsuan, and N. B. La Thangue. 1993. A new component of the transcription factor DRTF1/E2F. Nature (London) 362:83–87.
  • Goldsmith, M. E., and K. H. Cowan. 1988. Modulation of a human dihydrofolate reductase minigene following release from amino acid deprivation involves both 5′ and 3′ nucleotide sequences. Mol. Pharmacol. 33:378–383.
  • Hagemeier, C., A. Cook, and T. Kouzarides. 1993. The retinoblastoma protein binds E2F residues required for activation in vivo and TBP binding in vitro. Nucleic Acids Res. 21:4998–5004.
  • Helin, K., J. A. Lees, M. Vidal, N. Dyson, E. Harlow, and A. Fattaey. 1992. A cDNA encoding a pRB-binding protein with properties of transcription factor E2F. Cell 70:337–350.
  • Helin, K., C. L. Wu, A. R. Fattaey, J. A. Lees, B. D. Dynlacht, C. Ngwu, and E. Harlow. 1993. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Genes Dev. 7:1850–1861.
  • Hsiao, K.-M., S. L. McMahon, and P. J. Farnham. 1994. Multiple DNA elements are required for the growth regulation of the mouse E2F1 promoter. Genes Dev. 8:1526–1537.
  • Huber, H. E., G. Edwards, P. J. Goodhart, D. R. Patrick, P. S. Huang, M. Ivey-Hoyle, S. F. Barnett, A. Oliff, and D. C. Heimbrook. 1993. Transcription factor E2F binds DNA as a heterodimer. Proc. Natl. Acad. Sci. USA 90: 3525–3529.
  • Ivey-Hoyle, M., R. Conroy, H. E. Huber, P. J. Goodhart, A. Oliff, and D. C. Heimbrook. 1993. Cloning and characterization of E2F-2, a novel protein with the biochemical properties of transcription factor E2F. Mol. Cell. Biol. 13:7802–7812.
  • Johnson, D. G., K. Ohtani, and J. R. Nevins. 1994. Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression. Genes Dev. 8:1514–1525.
  • Johnson, L. F., C. L. Fuhrman, and L. M. Wiedemann. 1978. Regulation of dihydrofolate reductase gene expression in mouse fibroblasts during the transition from the resting to growing state. J. Cell. Physiol. 97:397–406.
  • Kadonaga, J. T., K. R. Carner, F. R. Masiarz, and R. Tijan. 1987. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell 51:1079–1090.
  • Kaelin, W. G., Jr., W. Krek, W. R. Sellers, J. A. DeCaprio, F. Ajchenbaum, C. S. Fuchs, T. Chittenden, Y. Li, P. J. Farnham, M. A. Blanar, D. M. Livingston, and E. K. Fleming. 1992. Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. Cell 70:351–364.
  • Krek, W., M. E. Ewen, S. Shirodkar, Z. Arany, W. G. Kaelin, Jr., and D. M. Livingston. 1994. Negative regulation of the growth-promoting transcription factor E2F-1 by a stably bound cyclin A-dependent protein kinase. Cell 78: 161–172.
  • La Thangue, N. B. .. 1994. DRTF1/E2F: an expanding family of heterodimeric transcription factors implicated in cell-cycle control. Trends Biochem. Sci. 19:108–114.
  • Lees, E., B. Faha, V. Dulic, S. I. Reed, and E. Harlow. 1992. Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner. Genes Dev. 6:1874–1885.
  • Lees, J. A., M. Saito, M. Vidal, M. Valentine, T. Look, E. Harlow, N. Dyson, and K. Helin. 1993. The retinoblastoma protein binds to a family of E2F transcription factors. Mol. Cell. Biol. 13:7813–7825.
  • Linton, J. P., J.-Y. J. Yen, E. Selby, Z. Chen, J. M. Chinsky, K. Liu, R. E. Kellems, and G. F. Crouse. 1989. Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene. Mol. Cell. Biol. 9:3058–3072.
  • McGrogan, M., C. C. Simonsen, D. T. Smouse, P. J. Farnham, and R. T. Schimke. 1985. Heterogeneity at the 5′ termini of mouse dihydrofolate reductase mRNAs. J. Biol. Chem. 260:2307–2314.
  • Means, A. L., J. E. Slansky, S. L. McMahon, M. W. Knuth, and P. J. Farnham. 1992. The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter. Mol. Cell. Biol. 12:1054–1063.
  • Milbrandt, J. D., N. H. Heintz, W. C. White, S. M. Rothman, and J. L. Hamlin. 1981. Methotrexate-resistant Chinese hamster ovary cells have amplified a 135-kilobase-pair region that includes the gene for dihydrofolate reductase. Proc. Natl. Acad. Sci. USA 78:6042–6047.
  • Mitchell, P. J., A. M. Carothers, J. H. Han, J. D. Harding, E. Kas, L. Venolia, and L. A. Chasin. 1986. Multiple transcription start sites, DNase I-hyper-sensitive sites, and an opposite-strand exon in the 5′ region of the CHO dhfr gene. Mol. Cell. Biol. 6:425–440.
  • Mudryj, M., S. H. Devoto, S. W. Hiebert, T. Hunter, J. Pines, and J. R. Nevins. 1991. Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A. Cell 65:1243–1253.
  • Neuman, E., E. K. Flemington, W. R. Sellers, and W. G. Kaelin, Jr. 1994. Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter. Mol Cell. Biol. 14:6607–6615.
  • Pemov, A., S. Bavykin, and J. L. Hamlin. 1995. Proximal and long-range alterations in chromatin structure surrounding the Chinese hamster dihydrofolate reductase promoter. Biochemistry 34:2381–2392.
  • Pfeifer, G. P., and A. D. Riggs. 1991. Chromatin differences between active and inactive X-chromosomes revealed by genomic footprinting of permeabilized cells using DNase I and ligation mediated PCR. Genes Dev. 5:1102–1113.
  • Pierce, A. J., R. C. Jambou, D. E. Jensen, and J. C. Azizkhan. 1992. A conserved DNA structural control element modulates transcription of a mammalian gene. Nucleic Acids Res. 20:6583–6587.
  • Potter, M., K. K. Sanford, R. Parshad, R. E. Tarone, F. M. Price, B. Mock, and K. Huppi. 1988. Genes on chromosomes 1 and 4 in the mouse are associated with repair of radiation-induced chromatin damage. Genomics 2: 257–262.
  • Pugh, B. F., and R. Tjian. 1991. Transcription from a TATA-less promoter requires a multisubunit TFIID complex. Genes Dev. 5:1935–1945.
  • Roy, A. L., C. Carruthers, T. Gutjahr, and R. G. Roeder. 1993. Direct role for Myc in transcription initiation mediated interactions by TFII-I. Nature (London) 365:359–361.
  • Santiago, C., M. Collins, and L. F. Johnson. 1984. In vitro and in vivo analysis of the control of dihydrofolate reductase gene transcription in serum-stimulated mouse fibroblasts. J. Cell. Physiol. 118:79–86.
  • Schilling, L. J., and P. J. Farnham. 1994. Transcriptional regulation of the dihydrofolate reductase/rep3 locus. Crit. Rev. Eukaryotic Gene Expr. 4:19–53.
  • Schilling, L. J., and P. J. Farnham. 1989. Identification of a new promoter upstream of the murine dihydrofolate reductase gene. Mol. Cell. Biol. 9: 4568–4570.
  • Shimada, T., K. Inokuchi, and A. W. Nienhuis. 1986. Chromatin structure of the human dihydrofolate reductase gene promoter. J. Biol. Chem. 261: 1445–1452.
  • Shull, S., N. H. Heintz, M. Perisamy, M. Manohar, Y. M. W. Janssen, J. P. Marsh, and B. T. Mossman. 1991. Differential regulation of antioxidant enzymes in response to oxidants. J. Biol. Chem. 266:24398–24403.
  • Slansky, J. E., Y. Li, W. G. Kaelin, and P. J. Farnham. 1993. A protein synthesis-dependent increase in E2F1 mRNA correlates with growth regulation of the dihydrofolate reductase promoter. Mol. Cell. Biol. 13:1610–1618.
  • Smith, M. L., P. J. Mitchell, and G. F. Crouse. 1990. Analysis of the mouse Dhfr/Rep-3 major promoter region by using linker-scanning and internal deletion mutations and DNase I footprinting. Mol. Cell. Biol. 10:6003–6012.
  • Stubblefield, E., and R. Klevecz. 1965. Synchronization of Chinese hamster cells by reversal of colcemid inhibition. Exp. Cell Res. 40:660–664.
  • Swick, A. G., M. C. Blake, J. W. Kahn, and J. C. Azizkhan. 1989. Functional analysis of GC element binding and transcription in the hamster dihydrofolate reductase gene promoter. Nucleic Acids Res. 17:9291–9303.
  • Wade, M., M. C. Blake, R. C. Jambous, K. Helin, E. Harlow, and J. C. Azizkhan. 1995. An inverted repeat motif stabilizes binding of E2F and enhances transcription of the dihydrofolate reductase gene. J. Biol. Chem. 270:9783–9791.
  • Weintraub, S. J., K. N. B. Chow, R. X. Luo, S. H. Zhang, S. He, and D. C. Dean. 1995. Mechanism of active transcriptional repression by the retinoblastoma protein. Nature (London) 375:812–815.
  • Wells, J., and N. H. Heintz. Unpublished data.
  • Wells, J., J. Magae, S. Illenye, and N. H. Heintz. Unpublished data.
  • Wiley, S. R., R. J. Kraus, and J. E. Mertz. 1992. Functional binding of the “TATA” box binding component of transcription factor TFIID to the –30 region of TATA-less promoters. Proc. Natl. Acad. Sci. USA 89:5814–5818.
  • Wu, C.-L., L. R. Zukerberg, C. Ngwu, E. Harlow, and J. A. Lees. 1995. In vivo association of E2F and DP family proteins. Mol. Cell. Biol. 15:2536–2546.
  • Zawel, L., and D. Reinberg. 1993. Initiation of transcription by RNA polymerase II: a multistep process. Prog. Nucleic Acid Res. Mol. Biol. 44:67–108.
  • Zhu, L., L. Zhu, E. Xie, and L.-S. Chang. 1995. Differential roles of two tandem E2F sites in repression of the human p107 promoter by retinoblastoma and p107 proteins. Mol. Cell. Biol. 15:3552–3562.

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