Phosphorylation of Raf-1 Serine 338-Serine 339 Is an Essential Regulatory Event for Ras-Dependent Activation and Biological Signaling

REFERENCES

• Avruch, J. Personal communication.
   Google Scholar
• Avruch, J., X. F. Zhang, and J. M. Kyriakis. 1994. Raf meets Ras: completing the framework of a signal transduction pathway. Trends Biochem. Sci. 19:279–283.
   PubMed Web of Science ®Google Scholar
• Barbacid, M. 1987. ras genes. Annu. Rev. Biochem. 56:779–827.
   PubMed Web of Science ®Google Scholar
• Bos, J. L. 1989. Ras oncogenes in human cancer: a review. Cancer Res. 49:4682–4689.
   PubMed Web of Science ®Google Scholar
• Boyle, W. J., P. van der Geer, and T. Hunter. 1991. Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates. Methods Enzymol. 201:110–149.
   PubMed Web of Science ®Google Scholar
• Brtva, T. R., J. K. Drugan, S. Ghosh, R. S. Terrell, S. Campbell-Burk, R. M. Bell, and C. J. Der. 1995. Two distinct Raf domains mediate interaction with Ras. J. Biol. Chem. 270:9809–9812.
   PubMed Web of Science ®Google Scholar
• Chu, G., H. Hayakawa, and P. Berg. 1987. Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 15:1311–1326.
   PubMed Web of Science ®Google Scholar
• Chuang, E., D. Barnard, L. Hettich, X. Zhang, J. Avruch, and M. Marshall. 1994. Critical binding and regulatory interactions between Ras and Raf occur through a small, stable N-terminal domain of Raf and specific Ras effector residues. Mol. Cell. Biol. 14:5318–5325.
   PubMedGoogle Scholar
• Crews, C. M., and R. L. Erikson. 1993. Extracellular signals and reversible protein phosphorylation: what to Mek of it all. Cell 74:215–217.
   PubMed Web of Science ®Google Scholar
• Dent, P., T. Jelinek, D. K. Morrison, M. J. Weber, and T. W. Sturgill. 1995. Reversal of Raf-1 activation by purified and membrane-associated protein phosphatases. Science 268:1902–1906.
   PubMed Web of Science ®Google Scholar
• Dent, P., D. B. Reardon, D. K. Morrison, and T. W. Sturgill. 1995. Regulation of Raf-1 and Raf-1 mutants by Ras-dependent and Ras-independent mechanisms in vitro. Mol. Cell. Biol. 15:4125–4135.
   PubMedGoogle Scholar
• Donella-Deana, A., O. Marin, A. M. Brunati, L. Cesaro, C. Piutti, and L. A. Pinna. 1993. Phosphorylated residues as specificity determinants for an acidophilic protein tyrosine kinase. FEBS Lett. 330:141–145.
   PubMedGoogle Scholar
• Evan, G. I., G. K. Lewis, G. Ramsay, and J. M. Bishop. 1985. Isolation of monoclonal antibodies specific for human c-Myc proto-oncogene product. Mol. Cell. Biol. 5:3610–3616.
   PubMed Web of Science ®Google Scholar
• Fabian, J. R., I. O. Daar, and D. K. Morrison. 1993. Critical tyrosine residues regulate the enzymatic and biological activity of Raf-1 kinase. Mol. Cell. Biol. 13:7170–7179.
   PubMed Web of Science ®Google Scholar
• Freed, E., M. Symons, S. G. Macdonald, F. McCormick, and R. Ruggieri. 1994. Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation. Science 265:1713–1716.
   PubMed Web of Science ®Google Scholar
• Hancock, J. F., K. Cadwallader, H. Paterson, and C. J. Marshall. 1991. A CAAX or a CAAL motif and a second signal are sufficient for plasma membrane targeting of Ras proteins. EMBO J. 10:4033–4039.
   PubMed Web of Science ®Google Scholar
• Heidecker, G., M. Huleihel, J. L. Cleveland, W. Kolch, T. W. Beck, P. Lloyd, T. Pawson, and U. R. Rapp. 1990. Mutational activation of c-raf-1 and definition of the minimal transforming sequence. Mol. Cell. Biol. 10:2503–2512.
   PubMed Web of Science ®Google Scholar
• Heidecker, G., W. Kolch, D. K. Morrison, and U. R. Rapp. 1992. The role of Raf-1 phosphorylation in signal transduction. Adv. Cancer Res. 58:53–73.
   PubMedGoogle Scholar
• Hu, C., K. Kariya, M. A. Tamada, K. M. Shirouzu, S. Yokoyama, and T. Kataoka. 1995. Cysteine-rich region of Raf-1 interacts with activator domain of post-translationally modified Ha-Ras. J. Biol. Chem. 270:30274–30277.
   PubMed Web of Science ®Google Scholar
• Jelinek, T., P. Dent, T. W. Sturgill, and M. J. Weber. 1996. Ras-induced activation of Raf-1 is dependent on tyrosine phosphorylation. Mol. Cell. Biol. 16:1027–1034.
   PubMed Web of Science ®Google Scholar
• Kolch, W., G. Heidecker, G. Kochs, R. Hummel, H. Vahidi, H. Mischak, G. Finkenzeller, D. Marme, and U. R. Rapp. 1993. Protein kinase C activates Raf-1 by direct phosphorylation. Nature 364:249–252.
   PubMed Web of Science ®Google Scholar
• Kolodziej, P. A., and R. A. Young. 1991. Epitope tagging and protein surveillance. Methods Enzymol. 194:508–519.
   PubMed Web of Science ®Google Scholar
• Kyriakis, J. M., H. App, X.-F. Zhang, P. Banerjee, D. L. Brautigan, U. R. Rapp, and J. Avruch. 1992. Raf-1 activates MAP kinase-kinase. Nature 358:417–421.
   PubMed Web of Science ®Google Scholar
• Leevers, S. J., H. F. Paterson, and C. J. Marshall. 1994. Requirement for Ras in Raf activation is overcome by targeting Raf to the plasma membrane. Nature 369:411–414.
   PubMed Web of Science ®Google Scholar
• Luo, Z., G. Tzivion, P. J. Belshaw, M. S. Marshall, and M. S. Avruch. 1996. Oligomerization activates c-Raf-1 through a Ras-dependent mechanism. Nature 383:181–185.
   PubMedGoogle Scholar
• MacDonald, S. G., C. M. Crews, L. Wu, J. Driller, R. Clark, R. L. Erikson, and F. McCormick. 1993. Reconstitution of the Raf-1-MEK-ERK signal transduction pathway in vitro. Mol. Cell. Biol. 13:6615–6620.
   PubMed Web of Science ®Google Scholar
• Marais, R., Y. Light, H. F. Paterson, and C. J. Marshall. 1995. Ras recruits Raf-1 to the plasma membrane for activation by tyrosine phosphorylation. EMBO J. 14:3136–3145.
   PubMed Web of Science ®Google Scholar
• Marshall, M. S. 1995. Ras target proteins in eukaryotic cells. FASEB J. 9:1311–1318.
   PubMed Web of Science ®Google Scholar
• Miller, J., and R. N. Germain. 1986. Efficient cell surface expression of class II MHC molecules in the absence of associated invariant chain. J. Exp. Med. 164:1478–1489.
   PubMed Web of Science ®Google Scholar
• Mischak, H., T. Seitz, P. Janosch, M. Eulitz, H. Steen, M. Schellerer, A. Philipp, and W. Kolch. 1996. Negative regulation of Raf-1 by phosphorylation of serine 621. Mol. Cell. Biol. 16:5409–5418.
   PubMedGoogle Scholar
• Morgenstern, J. P., and H. Land. 1990. Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 18:3587–3596.
   PubMed Web of Science ®Google Scholar
• Morrison, D. K. 1996. Mechanisms regulating Raf-1 activity in signal transduction pathways. Mol. Reprod. Dev. 42:507–514.
   Google Scholar
• Morrison, D. K., G. Heidecker, U. R. Rapp, and T. D. Copeland. 1993. Identification of the major phosphorylation sites of the Raf-1 kinase. J. Biol. Chem. 268:17309–17316.
   PubMed Web of Science ®Google Scholar
• Muslin, A. J., J. W. Tanner, P. M. Allen, and A. S. Shaw. 1996. Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine. Cell 84:889–897.
   PubMed Web of Science ®Google Scholar
• Schramm, K., M. Niehof, G. Radziwill, C. Rommel, and K. Moelling. 1994. Phosphorylation of c-Raf-1 by protein kinase A interferes with activation. Biochem. Biophys. Res. Commun. 201:740–747.
   PubMedGoogle Scholar
• Schulte, T. W., M. V. Blagosklonny, C. Ingui, and L. Neckers. 1995. Disruption of the Raf-1-Hsp90 molecular complex results in destabilization of Raf-1 and loss of Raf-1-Ras association. J. Biol. Chem. 270:24585–24588.
   PubMed Web of Science ®Google Scholar
• Songyang, Z., K. L. Carraway III, M. J. Eck, S. C. Harrison, R. A. Feldman, M. Mohammadi, J. Schlessinger, S. R. Hubbard, D. P. Smith, C. Eng, M. J. Lorenzo, B. A. J. Ponder, B. J. Mayer, and L. C. Cantley. 1995. Catalytic specificity of protein-tyrosine kinases is critical for selective signalling. Nature 373:536–539.
   PubMed Web of Science ®Google Scholar
• Stokoe, D., S. G. Macdonald, K. Cadwallader, M. Symons, and J. F. Hancock. 1994. Activation of Raf as a result of recruitment to the plasma membrane. Science 264:1463–1467.
   PubMed Web of Science ®Google Scholar
• Traverse, S., P. Cohen, H. Paterson, C. Marshall, U. Rapp, and R. J. Grand. 1993. Specific association of activated MAP kinase kinase kinase (Raf) with the plasma membranes of ras-transformed retinal cells. Oncogene 8:3175–3181.
   PubMedGoogle Scholar