Synthetic Lethality of Yeast slt Mutations with U2 Small Nuclear RNA Mutations Suggests Functional Interactions between U2 and U5 snRNPs That Are Important for Both Steps of Pre-mRNA Splicing

REFERENCES

• Ansari, A., and B. Schwer 1994. SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing. EMBO J. 14: 4001–4009.
   Google Scholar
• Arenas, J. E., and J. N. Abelson 1997. Prp43: an RNA helicase-like factor involved in spliceosome disassembly. Proc. Natl. Acad. Sci. USA 94: 11798–11802.
   PubMed Web of Science ®Google Scholar
• Ast, G., and A. M. Weiner 1996. A U1/U4/U5 snRNP complex induced by a 2′-O-methyl-oligoribonucleotide complementary to U5 snRNA. Science 272: 881–884.
   PubMedGoogle Scholar
• Ayadi, L., M. Miller, and J. Banroques 1997. Mutations within the yeast U4/U6 snRNP protein Prp4 affect a late stage of spliceosome assembly. RNA 3: 197–209.
   PubMedGoogle Scholar
• Brow, D. A., and R. M. Vidaver 1995. An element in human U6 RNA destabilizes the U4/U6 spliceosomal RNA complex. RNA 1: 122–131.
   PubMed Web of Science ®Google Scholar
• Brys, A., and B. Schwer 1996. Requirement for SLU7 in yeast pre-mRNA splicing is dictated by the distance between the branchpoint and the 3′ splice site. RNA 2: 707–717.
   PubMedGoogle Scholar
• Burgess, S., J. R. Couto, and C. Guthrie 1990. A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. Cell 60: 705–717.
   PubMed Web of Science ®Google Scholar
• Burgess, S. M., and C. Guthrie 1993. A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates. Cell 73: 1377–1391.
   PubMed Web of Science ®Google Scholar
• Chiara, M. D., L. Palandjian, R. Feld Kramer, and R. Reed 1997. Evidence that U5 snRNP recognizes the 3′ splice site for catalytic step II in mammals. EMBO J. 16: 4746–4759.
   PubMedGoogle Scholar
• Chin, K., and A. M. Pyle 1995. Branch-point attack in group II introns is a highly reversible transesterification, providing a potential proofreading mechanism for 5′-splice site selection. RNA 1: 391–406.
   PubMed Web of Science ®Google Scholar
• Fabrizio, P., and J. Abelson 1990. Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing. Science 250: 404–409.
   PubMedGoogle Scholar
• Field, D. J., and J. D. Friesen 1996. Functionally redundant interactions between U2 and U6 spliceosomal snRNAs. Genes Dev. 10: 489–501.
   PubMed Web of Science ®Google Scholar
• Frank, D., and C. Guthrie 1992. An essential splicing factor, SLU7, mediates 3′ splice site choice in yeast. Genes Dev. 6: 2112–2124.
   PubMedGoogle Scholar
• Frank, D., B. Patterson, and C. Guthrie 1992. Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. Mol. Cell. Biol. 12: 5197–5205.
   PubMed Web of Science ®Google Scholar
• Harris-Kerr, C. L., M. Zhang, and C. L. Peebles 1993. The phylogenetically predicted base-pairing interaction between alpha and alpha′ is required for group II splicing in vitro. Proc. Natl. Acad. Sci. USA 90: 10658–10662.
   PubMedGoogle Scholar
• Hetzer, M., G. Wurzer, R. J. Schweyen, and M. W. Mueller 1997. Trans-activation of group II intron splicing by nuclear U5 snRNA. Nature 386: 417–420.
   PubMedGoogle Scholar
• Hu, J., Y. Xu, K. Schappert, T. Harrington, A. Wang, R. Braga, J. Mogridge, and J. D. Friesen 1994. Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions. Nucleic Acids Res. 22: 1724–1734.
   PubMedGoogle Scholar
• Jacquier, A., and N. Jacquesson-Breuleux 1991. Splice site selection and role of the lariat in a group II intron. J. Mol. Biol. 219: 415–428.
   PubMed Web of Science ®Google Scholar
• Jacquier, A., and F. Michel 1987. Multiple exon-binding sites in class II self-splicing introns. Cell 50: 17–29.
   PubMedGoogle Scholar
• Jones, M. H., D. N. Frank, and C. Guthrie 1995. Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast. Proc. Natl. Acad. Sci. USA 92: 9687–9691.
   PubMedGoogle Scholar
• Kandels-Lewis, S., and B. Seraphin 1993. Involvement of U6 snRNA in 5′ splice site selection. Science 262: 2035–2039.
   PubMedGoogle Scholar
• Lauber, J., P. Fabrizio, S. Teigelkamp, W. S. Lane, E. Hartmann, and R. Luhrmann 1996. The HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases. EMBO J. 15: 4001–4015.
   PubMedGoogle Scholar
• Lesser, C. F., and C. Guthrie 1993. Mutations in U6 snRNA that alter splice site specificity: implications for the active site. Science 262: 1982–1988.
   PubMedGoogle Scholar
• Lin, R. J., A. J. Newman, S.-C. Cheng, and J. Abelson 1985. Yeast mRNA splicing in vitro. J. Biol. Chem. 260: 14780–14792.
   PubMedGoogle Scholar
• MacMillan, A. M., C. C. Query, C. R. Allerson, S. Chen, G. L. Verdine, and P. A. Sharp 1994. Dynamic association of proteins with the pre-mRNA branch region. Genes Dev. 8: 3008–3020.
   PubMed Web of Science ®Google Scholar
• Madhani, H. D., and C. Guthrie 1994. Dynamic RNA-RNA interactions in the spliceosome. Annu. Rev. Genet. 28: 1–26.
   PubMedGoogle Scholar
• Madhani, H. D., and C. Guthrie 1992. A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 71: 803–817.
   PubMed Web of Science ®Google Scholar
• Madhani, H. D., and C. Guthrie 1994. Randomization-selection analysis of snRNAs in vivo: evidence for a tertiary interaction in the spliceosome. Genes Dev. 8: 1071–1086.
   PubMed Web of Science ®Google Scholar
• McPheeters, D. S. 1996. Interactions of the yeast U6 RNA with the pre-mRNA branch site. RNA 2: 1110–1123.
   PubMedGoogle Scholar
• McPheeters, D. S., and J. Abelson 1992. Mutational analysis of the yeast U2 snRNA suggests a structural similarity to the catalytic core of Group I introns. Cell 171: 819–831.
   Google Scholar
• Moore, M. J., C. C. Query, and P. A. Sharp 1993. Splicing of precursors to mRNA by the spliceosome 303–357Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Newman, A. J., and C. Norman 1992. U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites. Cell 68: 743–754.
   PubMed Web of Science ®Google Scholar
• Newman, A. J., S. Teigelkamp, and J. D. Beggs 1995. snRNA interactions at 5′ and 3′ splice sites monitored by photoactivated crosslinking in yeast spliceosomes. RNA 1: 968–980.
   PubMedGoogle Scholar
• Noble, S. M., and C. Guthrie 1996. Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics 143: 67–80.
   PubMed Web of Science ®Google Scholar
• O’Keefe, R. T., C. Norman, and A. J. Newman 1996. The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast. Cell 86: 679–689.
   PubMed Web of Science ®Google Scholar
• Parker, R., and P. G. Siliciano 1993. Evidence for an essential non-Watson-Crick interaction between the first and last nucleotides of a nuclear pre-mRNA intron. Nature 361: 660–662.
   PubMedGoogle Scholar
• Query, C. C., M. J. Moore, and P. A. Sharp 1994. Branch nucleophile selection in pre-mRNA splicing: evidence for the bulged duplex model. Genes Dev. 8: 587–597.
   PubMed Web of Science ®Google Scholar
• Rose, M. D., P. Novick, J. H. Thomas, D. Botstein, and G. R. Fink 1987. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237–243.
   PubMed Web of Science ®Google Scholar
• Roy, J., B. Zheng, B. C. Rymond, Woolford J. L., Jr. 1995. Structurally related but functionally distinct yeast SmD core small nuclear ribonucleoprotein particle proteins. Mol. Cell. Biol. 15: 445–455.
   PubMedGoogle Scholar
• Ruby, S. W., T. H. Chang, and J. Abelson 1993. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Genes Dev. 7: 1909–1925.
   PubMed Web of Science ®Google Scholar
• Rymond, B., and M. Rosbash 1992. Yeast pre-mRNA splicing 143–192Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Sawa, H., and J. Abelson 1992. Evidence for a base-pairing interaction between U6 small nuclear RNA and 5′ splice site during the splicing reaction in yeast. Proc. Natl. Acad. Sci. USA 89: 11269–11273.
   PubMedGoogle Scholar
• Schwer, B., and C. Guthrie 1992. A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. EMBO J. 11: 5033–5039.
   PubMed Web of Science ®Google Scholar
• Sontheimer, E. J., and J. A. Steitz 1993. The U5 and U6 small nuclear RNAs as active site components of the spliceosome. Science 262: 1989–1996.
   PubMed Web of Science ®Google Scholar
• Teigelkamp, S., A. J. Newman, and J. D. Beggs 1995. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA. EMBO J. 14: 2602–2612.
   PubMed Web of Science ®Google Scholar
• Umen, J. G., and C. Guthrie 1996. Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3′ splice site selection. Genetics 143: 723–739.
   PubMedGoogle Scholar
• Umen, J. G., and C. Guthrie 1995. A novel role for a U5 snRNP protein in 3′ splice site selection. Genes Dev. 9: 855–868.
   PubMedGoogle Scholar
• Umen, J. G., and C. Guthrie 1995. Prp16p, Slu7p, and Prp8p interact with the 3′ splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA 1: 584–597.
   PubMedGoogle Scholar
• Vijayraghavan, U., M. Company, and J. Abelson 1989. Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 3: 1206–1216.
   PubMed Web of Science ®Google Scholar
• Wassarman, D. A., and J. A. Steitz 1992. Interactions of small nuclear RNA’s with precursor messenger RNA during in vitro splicing. Science 257: 1918–1925.
   PubMedGoogle Scholar
• Wells, S. E., M. Neville, M. Haynes, J. Wang, H. Igel, Ares M., Jr. 1996. CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145. Genes Dev. 10: 220–232.
   PubMedGoogle Scholar
• Wyatt, J. R., E. J. Sontheimer, and J. A. Steitz 1992. Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing. Genes Dev. 6: 2542–2553.
   PubMed Web of Science ®Google Scholar
• Xu, D., S. Nouraini, D. Field, S. J. Tang, and J. D. Friesen 1996. An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature 381: 709–713.
   PubMed Web of Science ®Google Scholar
• Yan, D., and M. J. Ares 1996. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol. Cell. Biol. 16: 818–828.
   PubMedGoogle Scholar