Cytoplasmic Sequestration of the Polyomavirus Enhancer Binding Protein 2 (PEBP2)/Core Binding Factor α (CBFα) Subunit by the Leukemia-Related PEBP2/CBFβ-SMMHC Fusion Protein Inhibits PEBP2/CBF-Mediated Transactivation

REFERENCES

• Bae, S. C., E. Ogawa, M. Maruyama, H. Oka, M. Satake, K. Shigesada, N. A. Jenkins, D. J. Gilbert, N. G. Copeland, and Y. Ito 1994. PEBP2αB/mouse AML1 consists of multiple isoforms that possess differential transactivation potentials. Mol. Cell. Biol. 14: 3242–3252.
   PubMed Web of Science ®Google Scholar
• Cao, W., M. Britos-Bray, D. F. Claxton, C. A. Kelley, N. A. Speck, P. P. Liu, and A. D. Friedman 1997. CBFb-SMMHC, expressed in M4Eo AML, reduced CBF DNA-binding and inhibited the G1 to S cell cycle transition at the restriction point in myeloid and lymphoid cells. Oncogene 15: 1315–1327.
   PubMedGoogle Scholar
• Cao, Z., R. M. Umek, and S. L. MaKnight 1991. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes Dev. 5: 1538–1552.
   PubMed Web of Science ®Google Scholar
• Castilla, L. H., C. Wijmenga, Q. Wang, T. Stacy, N. A. Speck, M. Eckhaus, M. Marin-Padilla, F. S. Collins, A. Wynshaw-Boris, and P. P. Liu 1996. Failure of embryonic hematopoiesis and lethal hemorrhages in mouse embryos heterozygous for a knocked-in leukemia gene CBFB-MYH11. Cell 87: 687–696.
   PubMed Web of Science ®Google Scholar
• Erickson, P., J. Gao, K. S. Chang, T. Look, E. Whisenant, S. Raimondi, R. Lasher, J. Trujillo, J. Rowley, and H. Drabkin 1992. Identification of breakpoints in t(8;21) acute myelogenous leukemia and isolation of a fusion transcript, AML1/ETO, with similarity to Drosophila segmentation gene, runt. Blood 80: 1825–1831.
   PubMed Web of Science ®Google Scholar
• Frank, R., J. Zhang, H. Uchida, S. Meyers, S. W. Hiebert, and S. D. Nimer 1995. The AML1/ETO fusion protein blocks transactivation of the GM-CSF promoter by AML1B. Oncogene 11: 2667–2674.
   PubMed Web of Science ®Google Scholar
• Giguere, V., E. S. Ong, P. Segui, and R. M. Evans 1987. Identification of a receptor for the morphogen retinoic acid. Nature 330: 624–629.
   PubMed Web of Science ®Google Scholar
• Golling, G., J.-H. Li, M. Pepling, M. Stebbins, and J. P. Gergen 1996. Drosophila homologs of the proto-oncogene product PEBP2/CBFβ regulate the DNA-binding properties of Runt. Mol. Cell. Biol. 16: 932–942.
   PubMed Web of Science ®Google Scholar
• Golub, T. R., G. F. Barker, S. K. Bohlander, S. W. Hiebert, D. C. Ward, P. Bray-Ward, E. Morgan, S. C. Raimondi, J. D. Rowley, and D. G. Gilliland 1995. Fusion of the TEL gene on 12p13 to the AML1 gene on 21q22 in acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 92: 4917–4921.
   PubMed Web of Science ®Google Scholar
• Grignani, F., P. F. Ferrucci, U. Testa, G. Talamo, M. Fagioli, M. Alcalay, A. Mencarelli, C. Peschle, I. Nicoletti, and P. G. Pelicci 1993. The acute promyelocytic leukemia-specific PML-RARα fusion protein inhibits differentiation and promotes survival of myeloid precursor cells. Cell 74: 423–431.
   PubMed Web of Science ®Google Scholar
• Hodge, T. P., R. Cross, and J. Kendrick-Jones 1992. Role of the COOH-terminal nonhelical trailpiece in the assembly of a vertebrate nonmuscle myosin rod. J. Cell Biol. 118: 1085–1095.
   PubMed Web of Science ®Google Scholar
• Ito, Y., and S.-C. Bae The Runt domain transcription factor, PEBP2/CBF, and its involvement in human leukemia Oncogenes as transcriptional regulators In: Yaniv, M., and J. Ghysdael21997107–132Birkhauser Verlag, Basel, Switzerland.
   Google Scholar
• Kagoshima, H., Y. Akamatsu, Y. Ito, and K. Shigesada 1996. Functional dissection of the alpha and beta subunits of transcription factor PEBP2 and the redox susceptibility of its DNA binding activity. J. Biol. Chem. 271: 33074–33082.
   PubMedGoogle Scholar
• Kanno, T., Y. Kanno, L.-F. Chen, E. Ogawa, W.-Y. Kim, and Y. Ito 1998. Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/core binding factor α subunit revealed in the presence of the β subunit. Mol. Cell. Biol. 18: 2444–2454.
   PubMedGoogle Scholar
• Kanno, Y., T. Kanno, and Y. Ito. Unpublished data.
   Google Scholar
• Kendrick-Jones, J., R. C. Smith, R. Craig, and S. Citi 1987. Polymerization of vertebrate non-muscle and smooth muscle myosins. J. Mol. Biol. 198: 241–252.
   PubMedGoogle Scholar
• Kurokawa, M., T. Tanaka, K. Tanaka, S. Ogawa, K. Mitani, Y. Yazaki, and H. Hirai 1996. Overexpression of the AML1 proto-oncoprotein in NIH3T3 cells leads to neoplastic transformation depending on the DNA-binding and transactivational potencies. Oncogene 12: 883–892.
   PubMed Web of Science ®Google Scholar
• Liu, P., S. A. Tarle, A. Hajra, D. F. Claxton, P. Marlton, M. Freedman, M. J. Siciliano, and F. S. Collins 1993. Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia. Science 261: 1041–1044.
   PubMed Web of Science ®Google Scholar
• Look, A. T. 1997. Oncogenic transcription factors in the human acute leukemias. Science 278: 1059–1064.
   PubMed Web of Science ®Google Scholar
• Lu, J., M. Maruyama, M. Satake, S. C. Bae, E. Ogawa, H. Kagoshima, K. Shigesada, and Y. Ito 1995. Subcellular localization of the α and β subunits of the acute myeloid leukemia-linked transcription factor PEBP2/CBF. Mol. Cell. Biol. 15: 1651–1661.
   PubMed Web of Science ®Google Scholar
• Matsuoka, R., M. O. Yoshida, Y. Furutani, S. Imamura, N. Kanda, M. Yanagisawa, T. Masaki, and A. Takao 1993. Human smooth muscle myosin heavy chain gene mapped to chromosomal region 16q12. Am. J. Med. Genet. 46: 61–67.
   PubMed Web of Science ®Google Scholar
• Meyers, S., N. Lenny, and S. W. Hiebert 1995. The t(8;21) fusion protein interferes with AML-1B-dependent transcriptional activation. Mol. Cell. Biol. 15: 1974–1982.
   PubMed Web of Science ®Google Scholar
• Miyoshi, H., T. Kozu, K. Shimizu, K. Enomoto, N. Maseki, Y. Kaneko, N. Kamada, and M. Ohki 1993. The t(8;21) translocation in acute myeloid leukemia results in production of an AML1-MTG8 fusion transcript. EMBO J. 12: 2715–2721.
   PubMed Web of Science ®Google Scholar
• Mizushima, S., and S. Nagata 1990. pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res. 18: 5322.
   PubMed Web of Science ®Google Scholar
• Niki, M., H. Okada, H. Takano, J. Kuno, K. Tani, H. Hibino, S. Asano, Y. Ito, M. Satake, and T. Noda 1997. Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcriptional factor, polyomavirus enhancer binding protein 2/core binding factor. Proc. Natl. Acad. Sci. USA 94: 5697–5702.
   PubMedGoogle Scholar
• Ogawa, E., M. Inuzuka, M. Maruyama, M. Satake, M. Naito-Fujimoto, Y. Ito, and K. Shigesada 1993. Molecular cloning and characterization of PEBP2β, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2α. Virology 194: 314–331.
   PubMed Web of Science ®Google Scholar
• Ogawa, E., M. Maruyama, H. Kagoshima, M. Inuzuka, J. Lu, M. Satake, K. Shigesada, and Y. Ito 1993. PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene. Proc. Natl. Acad. Sci. USA 90: 6859–6863.
   PubMed Web of Science ®Google Scholar
• Okuda, T., J. van Deursen, S. W. Hiebert, G. Grosveld, and J. R. Downing 1996. AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis. Cell 84: 321–330.
   PubMed Web of Science ®Google Scholar
• Sakakura, C., Y. Yamaguchi-Iwai, M. Satake, S. C. Bae, A. Takahashi, E. Ogawa, A. Hagiwaga, T. Takahashi, A. Murakami, K. Makino, T. Nakagawa, N. Kamada, and Y. Ito 1994. Growth inhibition and induction of differentiation of t(8;21) acute myeloid leukemia cells by the DNA binding domain of PEBP2 and the AML1/MTG8(ETO) specific antisense oligonucleotide. Proc. Natl. Acad. Sci. USA 91: 11723–11727.
   PubMed Web of Science ®Google Scholar
• Sasaki, K., H. Yagi, R. T. Bronson, K. Tominaga, T. Matsunashi, K. Deguchi, Y. Tani, T. Kishimoto, and T. Komori 1996. Absence of fetal liver hematopoiesis in mice deficient in transcriptional coactivator core binding factor beta. Proc. Natl. Acad. Sci. USA 93: 12359–12363.
   PubMed Web of Science ®Google Scholar
• Shurtleff, S. A., S. Meyers, S. W. Hiebert, S. C. Raimondi, D. R. Head, C. L. Willman, S. Wolman, M. L. Slovak, A. J. Caroll, F. Behm, M. G. Hulshof, T. A. Motroni, T. Okuda, P. Liu, F. S. Collins, and J. R. Downing 1995. Heterogeneity in CBFβ/MYH11 fusion messages encoded by the inv(16)(p13q22) and the t(16;16)(p13;q22) in acute myelogenous leukemia. Blood 85: 3695–3703.
   PubMed Web of Science ®Google Scholar
• Speck, N. A., and T. Stacy 1995. A new transcription factor family associated with human leukemias. Crit. Rev. Eukaryotic Gene Expr. 5: 337–364.
   PubMed Web of Science ®Google Scholar
• Takahashi, A., M. Satake, Y. Yamagichi-Iwai, S. C. Bae, J. Lu, M. Maruyama, Y. W. Zhang, H. Oka, N. Arai, K. Arai, and Y. Ito 1995. Positive and negative regulation of granulocyte-macrophage colony-stimulating factor promoter activity by AML1-related transcription factor, PEBP2. Blood 86: 607–616.
   PubMed Web of Science ®Google Scholar
• Takebe, Y., M. Seiki, J. Fujisawa, P. Hoy, K. Yokota, K. Arai, M. Yoshida, and N. Arai 1988. SRα promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. 8: 466–472.
   PubMed Web of Science ®Google Scholar
• Testa, U., F. Grignani, T. Barberi, M. Fagioli, R. Masciulli, P. F. Ferruci, D. Seripa, A. Camagna, M. Alcalay, P. G. Pelicci, and C. Peschle 1994. PML/RARα+ U937 mutant and NB4 cell lines: retinoic acid restores the monocytic differentiation response to vitamin D3. Cancer Res. 54: 4508–4515.
   PubMed Web of Science ®Google Scholar
• Wang, S., Q. Wang, B. E. Crute, I. N. Melnikova, S. R. Keller, and N. A. Speck 1993. Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. Mol. Cell. Biol. 13: 3324–3339.
   PubMed Web of Science ®Google Scholar
• Wang, Q., T. Stacy, M. Binder, M. Marin-Padilla, A. H. Sharpe, and N. A. Speck 1996. Disruption of the cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. Proc. Natl. Acad. Sci. USA 93: 3444–3449.
   PubMed Web of Science ®Google Scholar
• Wang, Q., T. Stacy, J. D. Miller, A. F. Lewis, T.-L. Gu, X. Huang, J. H. Bushweller, J.-C. Bories, F. W. Alt, G. Ryan, P. P. Liu, A. Wynshaw-Boris, M. Binder, M. Marin-Padilla, A. H. Sharpe, and N. A. Speck 1996. The CBFβ subunit is essential for CBFα2 (AML1) function in vivo. Cell 87: 697–708.
   PubMed Web of Science ®Google Scholar
• Weis, K., S. Rambaud, C. Lavau, J. Jansen, T. Carvalho, M. Carmo-Fonseca, A. Lamond, and A. Dejean 1994. Retinoic acid regulates aberrant nuclear localization of PML-RARα in acute promyelocytic leukemia cells. Cell 76: 345–356.
   PubMed Web of Science ®Google Scholar
• Yanagisawa, K., T. Horiuchi, and S. Fujita 1991. Establishment and characterization of a new human leukemia cell line derived from M4Eo. Blood 78: 451–457.
   PubMed Web of Science ®Google Scholar
• Yergeau, D. A., C. J. Hetherington, Q. Wang, P. Zhang, A. H. Sharpe, M. Binder, M. Marin-Padilla, D. G. Tenen, N. A. Speck, and D. E. Zhang 1997. Embryonic lethality and impairment of haematopoiesis in mice heterozygous for an AML1-ETO fusion gene. Nat. Genet. 15: 303–306.
   PubMed Web of Science ®Google Scholar
• Zaiman, A. L., A. F. Lewis, B. E. Crute, N. A. Speck, and J. Lenz 1995. Transcriptional activity of core binding factor α (AML1) and β subunits on murine leukemia virus enhancer cores. J. Virol. 69: 2898–2906.
   PubMedGoogle Scholar
• Zhang, D. E., C. J. Hetherington, H. M. Chen, and D. G. Tenen 1994. The macrophage transcription factor PU.1 directs tissue-specific expression of the macrophage colony-stimulating factor receptor. Mol. Cell. Biol. 14: 373–381.
   PubMedGoogle Scholar
• Zhang, D. E., C. J. Hetherington, S. Meyers, K. L. Rhoades, C. J. Larson, H. M. Chen, S. W. Hiebert, and D. G. Tenen 1996. CCAAT enhancer-binding protein (C/EBP) and AML1 (CBF alpha2) synergistically activate the macrophage colony-stimulating factor receptor promoter. Mol. Cell. Biol. 16: 1231–1240.
   PubMed Web of Science ®Google Scholar
• Zhang, Y. W., S. C. Bae, G. Huang, Y. X. Fu, J. Lu, M. Y. Ahn, Y. Kanno, T. Kanno, and Y. Ito 1997. A novel transcript encoding an N-terminally truncated AML1/PEBP2αB protein interferes with transactivation and blocks granulocytic differentiation of 32Dcl3 myeloid cells. Mol. Cell. Biol. 17: 4133–4145.
   PubMedGoogle Scholar