Exonic Sequences in the 5′ Untranslated Region of α-Tubulin mRNA Modulate trans Splicing in Trypanosoma brucei

REFERENCES

• Bruzik, J. P., and T. Maniatis 1995. Enhancer-dependent interaction between 5′ and 3′ splice sites in trans. Proc. Natl. Acad. Sci. USA 92: 7056–7059.
   PubMedGoogle Scholar
• Chiara, M. D., and R. Reed 1995. A two-step mechanism for 5′ and 3′ splice-site pairing. Nature 375: 510–513.
   PubMedGoogle Scholar
• Coulter, L. R., M. A. Landree, and T. A. Cooper 1997. Identification of a new class of exonic splicing enhancers by in vivo selection. Mol. Cell. Biol. 17: 2143–2150 (Erratum, 17:3468.)
   PubMed Web of Science ®Google Scholar
• Dungan, J. M., K. P. Watkins, and N. Agabian 1996. Evidence for the presence of a small U5-like RNA in active trans-spliceosomes of Trypanosoma brucei. EMBO J. 15: 4016–4029.
   PubMed Web of Science ®Google Scholar
• Fantoni, A., A. O. Dare, and C. Tschudi 1994. RNA polymerase III-mediated transcription of the trypanosome U2 small nuclear RNA gene is controlled by both intragenic and extragenic regulatory elements. Mol. Cell. Biol. 14: 2021–2028.
   PubMedGoogle Scholar
• Goguel, V., and M. Rosbash 1993. Splice site choice and splicing efficiency are positively influenced by pre-mRNA intramolecular base pairing in yeast. Cell 72: 893–901.
   PubMed Web of Science ®Google Scholar
• Hertel, K. J., K. W. Lynch, and T. Maniatis 1997. Common themes in the function of transcription and splicing enhancers. Curr. Opin. Cell Biol. 9: 350–357.
   PubMedGoogle Scholar
• Hotz, H. R., C. Hartmann, K. Huober, M. Hug, and C. Clayton 1997. Mechanisms of developmental regulation in Trypanosoma brucei: a polypyrimidine tract in the 3′-untranslated region of a surface protein mRNA affects RNA abundance and translation. Nucleic Acids Res. 25: 3017–3026.
   PubMedGoogle Scholar
• Huang, J., and L. H. Van der Ploeg 1991. Requirement of a polypyrimidine tract for trans-splicing in trypanosomes: discriminating the PARP promoter from the immediately adjacent 3′ splice acceptor site. EMBO J. 10: 3877–3885.
   PubMedGoogle Scholar
• Hug, M., H. R. Hotz, C. Hartmann, and C. Clayton 1994. Hierarchies of RNA-processing signals in a trypanosome surface antigen mRNA precursor. Mol. Cell. Biol. 14: 7428–7435.
   PubMedGoogle Scholar
• Kuersten, S., K. Lea, M. MacMorris, J. Spieth, and T. Blumenthal 1997. Relationship between 3′ end formation and SL2-specific trans-splicing in polycistronic Caenorhabditis elegans pre-mRNA processing. RNA 3: 269–278.
   PubMedGoogle Scholar
• LeBowitz, J. H., H. Q. Smith, L. Rusche, and S. M. Beverley 1993. Coupling of poly(A) site selection and trans-splicing in Leishmania. Genes Dev. 7: 996–1007.
   PubMed Web of Science ®Google Scholar
• Libri, D., F. Stutz, T. McCarthy, and M. Rosbash 1995. RNA structural patterns and splicing: molecular basis for an RNA-based enhancer. RNA 1: 425–436.
   PubMedGoogle Scholar
• Matthews, K. R., C. Tschudi, and E. Ullu 1994. A common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes. Genes Dev. 8: 491–501.
   PubMed Web of Science ®Google Scholar
• McNally, K. P., and N. Agabian 1992. Trypanosoma brucei spliced-leader RNA methylations are required for trans splicing in vivo. Mol. Cell. Biol. 12: 4844–4851.
   PubMedGoogle Scholar
• Muhich, M. L., and J. C. Boothroyd 1988. Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis. Mol. Cell. Biol. 8: 3837–3846.
   PubMed Web of Science ®Google Scholar
• Patterson, B., and C. Guthrie 1991. A U-rich tract enhances usage of an alternative 3′ splice site in yeast. Cell 64: 181–187.
   PubMedGoogle Scholar
• Pays, E., L. Vanhamme, and M. Berberof 1994. Genetic controls for the expression of surface antigens in African trypanosomes. Annu. Rev. Microbiol. 48: 25–52.
   PubMedGoogle Scholar
• Schurch, N., A. Hehl, E. Vassella, R. Braun, and I. Roditi 1994. Accurate polyadenylation of procyclin mRNAs in Trypanosoma brucei is determined by pyrimidine-rich elements in the intergenic regions. Mol. Cell. Biol. 14: 3668–3675.
   PubMed Web of Science ®Google Scholar
• Tschudi, C., and E. Ullu 1988. Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes. EMBO J. 7: 455–463.
   PubMedGoogle Scholar
• Ullu, E. Unpublished data.
   Google Scholar
• Ullu, E., K. R. Matthews, and C. Tschudi 1993. Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts. Mol. Cell. Biol. 13: 720–725.
   PubMed Web of Science ®Google Scholar
• Ullu, E., and C. Tschudi 1991. Trans splicing in trypanosomes requires methylation of the 5′ end of the spliced leader RNA. Proc. Natl. Acad. Sci. USA 88: 10074–10078.
   PubMed Web of Science ®Google Scholar
• Ullu, E., C. Tschudi, and A. Günzl 1996. Trans-splicing in trypanosomatid protozoa Molecular biology of parasitic protozoa. In: Smith, D. F., and M. Parsons115–133Oxford University Press, Oxford, United Kingdom.
   Google Scholar
• Valcarcel, J., and M. R. Green 1996. The SR protein family: pleiotropic functions in pre-mRNA splicing. Trends Biochem. Sci. 21: 296–301.
   PubMedGoogle Scholar
• Vassella, E., R. Braun, and I. Roditi 1994. Control of polyadenylation and alternative splicing of transcripts from adjacent genes in a procyclin expression site: a dual role for polypyrimidine tracts in trypanosomes? Nucleic Acids Res. 22: 1359–1364.
   PubMed Web of Science ®Google Scholar
• Xu, Y., H. Ben-Shlomo, and S. Michaeli 1997. The U5 RNA of trypanosomes deviates from the canonical U5 RNA: the Leptomonas collosoma U5 RNA and its coding gene. Proc. Natl. Acad. Sci. USA 94: 8473–8478.
   PubMedGoogle Scholar
• Zomerdijk, J. C., R. Kieft, and P. Borst 1991. Efficient production of functional mRNA mediated by RNA polymerase I in Trypanosoma brucei. Nature 353: 772–775.
   PubMedGoogle Scholar