2
Views
111
CrossRef citations to date
0
Altmetric
Transcriptional Regulation

Multiple Cbfa/AML Sites in the Rat Osteocalcin Promoter Are Required for Basal and Vitamin D-Responsive Transcription and Contribute to Chromatin Organization

, , , , , & show all
Pages 7491-7500 | Received 25 Mar 1999, Accepted 05 Aug 1999, Published online: 28 Mar 2023

REFERENCES

  • Ahn, M. Y., S. C. Bae, M. Maruyama, and J. Ito 1996. Comparison of the human genomic structure of the runt domain-encoding PEBP2/CBFα gene family. Gene 168:279–280.
  • Archer, T. K., P. Lefebvre, R. G. Wolford, and J. Hager 1992. Transcription factor loading on the MMTV promoter: a bimodal mechanism for promoter activation. Science 255:1573–1576.
  • Aronson, B. D., A. L. Fisher, K. Blechman, M. Caudy, and J. Gergen 1997. Groucho-dependent and -independent repression activities of Runt domain proteins. Mol. Cell Biol. 17:5581–5587.
  • Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, K. Struhl 1997. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
  • Banerjee, C., S. W. Hiebert, J. L. Stein, J. B. Lian, and J. Stein 1996. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteocalcin gene. Proc. Natl. Acad. Sci. USA 93:4968–4973.
  • Banerjee, C., L. R. McCabe, J.-Y. Choi, S. W. Hiebert, J. L. Stein, G. S. Stein, and J. Lian 1997. Runt homology domain proteins in osteoblast differentiation: AML-3/CBFA1 is a major component of a bone specific complex. J. Cell. Biochem. 66:1–8.
  • Banerjee, C., J. L. Stein, A. J. van Wijnen, B. Frenkel, J. B. Lian, and J. Stein 1996. Transforming growth factor-beta 1 responsiveness of the rat osteocalcin gene is mediated by an activator protein-1 binding site. Endocrinology 137:1991–2000.
  • Beato, M. 1996. Chromatin structure and the regulation of gene expression: remodeling at the MMTV promoter. J. Mol. Med. 74:711–724.
  • Bidwell, J. P., A. J. van Wijnen, E. G. Fey, S. Dworetzky, S. Penman, J. L. Stein, J. B. Lian, and J. Stein 1993. Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components. Proc. Natl. Acad. Sci. USA 90:3162–3166.
  • Bruhn, L., A. Munnerlyn, and J. Grosschedl 1997. ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCRalpha enhancer function. Genes Dev. 11:640–653.
  • Clemens, T. L., H. Tang, S. Maeda, R. A. Kesterson, F. DeMayo, J. W. Pike, and J. Gundberg 1997. Analysis of osteocalcin expression in transgenic mice reveals a species difference in vitamin D regulation of mouse and human osteocalcin genes. J. Bone Miner. Res. 12:1570–1576.
  • Dignam, J. D., R. M. Lebovitz, and J. Roeder 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475–1489.
  • Ducy, P., and J. Karsenty 1995. Two distinct osteoblast-specific cis-acting elements control expression of a mouse osteocalcin gene. Mol. Cell. Biol. 15:1858–1869.
  • Ducy, P., R. Zhang, V. Geoffroy, A. L. Ridall, and J. Karsenty 1997. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 89:747–754.
  • Fisher, A. L., and J. Caudy 1998. Groucho proteins: transcriptional corepressors for specific subsets of DNA-binding transcription factors in vertebrates and invertebrates. Genes Dev. 12:1931–1940.
  • Fragoso, G., W. D. Pennie, S. John, and J. Hager 1998. The position and length of the steroid-dependent hypersensitive region in the mouse mammary tumor virus long terminal repeat are invariant despite multiple nucleosome B frames. Mol. Cell. Biol. 18:3633–3644.
  • Frendo, J. L., G. Xiao, S. Fuchs, R. T. Franceschi, G. Karsenty, and J. Ducy 1998. Functional hierarchy between two OSE2 elements in the control of osteocalcin gene expression in vivo. J. Biol. Chem. 273:30509–30516.
  • Frenkel, B., J. Mijnes, M. A. Aronow, G. Zambetti, C. Banerjee, J. L. Stein, J. B. Lian, and J. Stein 1993. Position and orientation-selective silencer in protein-coding sequences of the rat osteocalcin gene. Biochemistry 32:13636–13643.
  • Frenkel, B., M. Montecino, J. Green, F. Aslam, R. Desai, C. Banerjee, J. L. Stein, J. B. Lian, and J. Stein 1996. Basal and vitamin D-responsive activity of the rat osteocalcin promoter in stably transfected osteosarcoma cells: requirement of upstream sequences for control by the proximal regulatory domain. Endocrinology 137:1080–1088.
  • Gao, Y. H., T. Shinki, T. Yuasa, H. Kataoka-Enomoto, T. Komori, T. Suda, and J. Yamaguchi 1998. Potential role of cbfa1, an essential transcriptional factor for osteoblast differentiation, in osteoclastogenesis: regulation of mRNA expression of osteoclast differentiation factor (ODF). Biochem. Biophys. Res. Commun. 252:697–702.
  • Guo, B., F. Aslam, A. J. van Wijnen, S. G. E. Roberts, B. Frenkel, M. Green, H. DeLuca, J. B. Lian, G. S. Stein, and J. Stein 1997. YY1 regulates VDR/RXR mediated transactivation of the vitamin D responsive osteocalcin gene. Proc. Natl. Acad. Sci. USA 94:121–126.
  • Guo, B., A. Javed, J. Green, J. L. Stein, J. B. Lian, A. J. van Wijnen, and J. Stein 1998. Groucho/TLE proteins associate with the nuclear matrix and repress Cbfa/AML mediated transactivation on osteocalcin promoter. Bone 23:S184 (Abstract.)
  • Haussler, M. R., C. A. Haussler, P. W. Jurutka, P. D. Thompson, J. C. Hsieh, L. S. Remus, S. H. Selznick, and J. Whitfield 1997. The vitamin D hormone and its nuclear receptor: molecular actions and disease states. J. Endocrinol. 154:S57–S73.
  • Hiebert, S. W., W. Sun, J. N. Davis, T. Golub, S. Shurtleff, A. Buijs, J. R. Downing, G. Grosveld, M. F. Roussell, D. G. Gilliland, N. Lenny, and J. Meyers 1996. The t(12;21) translocation converts AML-1B from an activator to a repressor of transcription. Mol. Cell. Biol. 16:1349–1355.
  • Hoaglin, D., F. Mosteller, J. Tukey 1983. Understanding robust and exploratory data analysis. John Wiley & Sons, New York, N.Y.
  • Hoffmann, H., J. Green, A. J. van Wijnen, J. L. Stein, G. S. Stein, and J. B. Lian. Expression screening of factors binding to the osteocalcin bone-specific promoter element OC Box I: isolation of a novel osteoblast differentiation-specific factor. J. Cell. Biochem., in press.
  • Hoffmann, H. M., T. L. Beumer, S. Rahman, L. R. McCabe, C. Banerjee, F. Aslam, J. A. Tiro, A. J. van Wijnen, J. L. Stein, G. S. Stein, and J. Lian 1996. Bone tissue-specific transcription of the osteocalcin gene: role of an activator osteoblast-specific complex and suppressor hox proteins that bind the OC box. J. Cell. Biochem. 61:310–324.
  • Hoffmann, H. M., K. M. Catron, A. J. van Wijnen, L. R. McCabe, J. B. Lian, G. S. Stein, and J. Stein 1994. Transcriptional control of the tissue-specific, developmentally regulated osteocalcin gene requires a binding motif for the Msx family of homeodomain proteins. Proc. Natl. Acad. Sci. USA 91:12887–12891.
  • Ito, Y. 1997. The runt protein and its companion PEBP2: a close link between this transcription factor and AML. Leukemia 11 (Suppl. 3):279–280.
  • Javed, A., S. Gutierrez, A. J. van Wijnen, G. S. Stein, J. L. Stein, and J. Lian 1998. Cbfa binding sites are required for hormonal responsiveness in the osteocalcin promoter. Bone 23:S196 (Abstract.)
  • Ji, C., S. Casinghino, D. J. Chang, Y. Chen, A. Javed, Y. Ito, S. W. Hiebert, J. B. Lian, G. S. Stein, T. L. McCarthy, and J. Centrella 1998. CBFa(AML/PEBP2)-related elements in the TGF-beta type I receptor promoter and expression with osteoblast differentiation. J. Cell Biochem. 69:353–363.
  • Kanno, T., Y. Kanno, L. F. Chen, E. Ogawa, W. Y. Kim, and J. Ito 1998. Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/core binding factor alpha subunit revealed in the presence of the beta subunit. Mol. Cell. Biol. 18:2444–2454.
  • Komori, T. 1998. Cbfa1, a transcription factor for osteoblast differentiation and bone formation. J. Bone Miner. Metab. 16:1–4.
  • Komori, T., H. Yagi, S. Nomura, A. Yamaguchi, K. Sasaki, K. Deguchi, Y. Shimizu, R. T. Bronson, Y.-H. Gao, M. Inada, M. Sato, R. Okamoto, Y. Kitamura, S. Yoshiki, and J. Kishimoto 1997. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89:755–764.
  • Lenny, N., S. Meyers, and J. Hiebert 1995. Functional domains of the t(8;21) fusion protein, AML-1/ETO. Oncogene 11:1761–1769.
  • Levanon, D., R. E. Goldstein, Y. Bernstein, H. Tang, D. Goldenberg, S. Stifani, Z. Paroush, and J. Groner 1998. Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors. Proc. Natl. Acad. Sci. USA 95:11590–11595.
  • Levanon, D., V. Negreanu, Y. Bernstein, I. Bar-Am, L. Avivi, and J. Groner 1994. AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization. Genomics 23:425–432.
  • Lian, J., C. Stewart, E. Puchacz, S. Mackowiak, V. Shalhoub, D. Collart, G. Zambetti, and J. Stein 1989. Structure of the rat osteocalcin gene and regulation of vitamin D-dependent expression. Proc. Natl. Acad. Sci. USA 86:1143–1147.
  • Lian, J. B., V. Shalhoub, F. Aslam, B. Frenkel, J. Green, M. Hamrah, G. S. Stein, and J. Stein 1997. Species-specific glucocorticoid and 1,25-dihydroxyvitamin D responsiveness in mouse MC3T3-E1 osteoblasts: dexamethasone inhibits osteoblast differentiation and vitamin D downregulates osteocalcin gene expression. Endocrinology 138:2117–2127.
  • Lian, J. B., G. S. Stein, J. L. Stein, A. J. van Wijnen 1998. Regulated expression of the bone-specific osteocalcin gene by vitamins and hormones, p. 443–509. In G. Litwack (ed.), Vitamins and hormones. Academic Press, San Diego, Calif.
  • McCabe, L. R., C. Banerjee, R. Kundu, R. J. Harrison, P. R. Dobner, J. L. Stein, J. B. Lian, and J. Stein 1996. Developmental expression and activities of specific fos and jun proteins are functionally related to osteoblast maturation: role of fra-2 and jun D during differentiation. Endocrinology 137:4398–4408.
  • McLean, R. A., W. L. Sanders, and J. Stroup 1991. A unified approach to mixed linear models. Am. Stat. 45:54–64.
  • Merriman, H. L., A. J. van Wijnen, S. Hiebert, J. P. Bidwell, E. Fey, J. Lian, J. Stein, and J. Stein 1995. The tissue-specific nuclear matrix protein, NMP-2, is a member of the AML/CBF/PEBP2/runt domain transcription factor family: interactions with the osteocalcin gene promoter. Biochemistry 34:13125–13132.
  • Meyers, S., N. Lenny, W.-H. Sun, and J. Hiebert 1996. AML-2 is a potential target for transcriptional regulation by the t(8;21) and t(12;21) fusion proteins in acute leukemia. Oncogene 13:303–312.
  • Montecino, M., B. Frenkel, J. Lian, J. Stein, and J. Stein 1996. Requirement of distal and proximal promoter sequences for chromatin organization of the osteocalcin gene in bone-derived cells. J. Cell. Biochem. 63:221–228.
  • Montecino, M., J. Lian, G. Stein, and J. Stein 1996. Changes in chromatin structure support constitutive and developmentally regulated transcription of the bone-specific osteocalcin gene in osteoblastic cells. Biochemistry 35:5093–5102.
  • Montecino, M., S. Pockwinse, J. Lian, G. Stein, and J. Stein 1994. DNase I hypersensitive sites in promoter elements associated with basal and vitamin D dependent transcription of the bone-specific osteocalcin gene. Biochemistry 33:348–353.
  • Mundlos, S., F. Otto, C. Mundlos, J. B. Mulliken, A. S. Aylsworth, S. Albright, D. Lindhout, W. G. Cole, W. Henn, J. H. M. Knoll, M. J. Owen, R. Mertelsmann, B. U. Zabel, and J. Olsen 1997. Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia. Cell 89:773–779.
  • Otto, F., A. P. Thornell, T. Crompton, A. Denzel, K. C. Gilmour, I. R. Rosewell, G. W. H. Stamp, R. S. P. Beddington, S. Mundlos, B. R. Olsen, P. B. Selby, and J. Owen 1997. Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell 89:765–771.
  • Palaparti, A., A. Baratz, and J. Stifani 1997. The Groucho/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3. J. Biol. Chem. 272:26604–26610.
  • Ryoo, H.-M., H. M. Hoffmann, T. L. Beumer, B. Frenkel, D. A. Towler, G. S. Stein, J. L. Stein, A. J. van Wijnen, and J. Lian 1997. Stage-specific expression of Dlx-5 during osteoblast differentiation: involvement in regulation of osteocalcin gene expression. Mol. Endocrinol. 11:1681–1694.
  • Selvamurugan, N., W. Y. Chou, A. T. Pearman, M. R. Pulumati, and J. Partridge 1998. Parathyroid hormone regulates the rat collagenase-3 promoter in osteoblastic cells through the cooperative interaction of the activator protein-1 site and the runt domain binding sequence. J. Biol. Chem. 273:10647–10657.
  • Siegel, S. 1956. Nonparametric statistics for the behavioral sciences. McGraw-Hill Book Company, New York, N.Y.
  • Smith, C. L., and J. Hager 1997. Transcriptional regulation of mammalian genes in vivo. A tale of two templates. J. Biol. Chem. 272:27493–27496.
  • Smith, C. L., H. Htun, R. G. Wolford, and J. Hager 1997. Differential activity of progesterone and glucocorticoid receptors on mouse mammary tumor virus templates differing in chromatin structure. J. Biol. Chem. 272:14227–14235.
  • Speck, N. A., and J. Stacy 1995. A new transcription factor family associated with human leukemias. Crit. Rev. Eukaryot. Gene Expr. 5:337–364.
  • Stein, G. S., J. B. Lian, A. J. van Wijnen, and J. Stein 1997. The osteocalcin gene: a model for multiple parameters of skeletal-specific transcriptional control. Mol. Biol. Rep. 24:185–196.
  • Stewart, M., A. Terry, M. Hu, M. O’Hara, K. Blyth, E. Baxter, E. Cameron, D. E. Onions, and J. Neil 1997. Proviral insertions induce the expression of bone-specific isoforms of PEBP2alphaA (CBFA1): evidence for a new myc collaborating oncogene. Proc. Natl. Acad. Sci. USA 94:8646–8651.
  • Tang, Y., R. H. Getzenberg, B. N. Vietmeier, M. R. Stallcup, M. Eggert, R. Renkawitz, and J. DeFranco 1998. The DNA-binding and τ2 transactivation domains of the rat glucocorticoid receptor constitute a nuclear matrix targeting signal. Mol. Endocrinol. 12:1420–1431.
  • Thirunavukkarasu, K., M. Mahajan, K. W. McLarren, S. Stifani, and J. Karsenty 1998. Two domains unique to osteoblast-specific transcription factor Osf2/Cbfa1 contribute to its transactivation function and its inability to heterodimerize with Cbf beta. Mol. Cell. Biol. 18:4197–4208.
  • Towler, D. A., C. D. Bennett, and J. Rodan 1994. Activity of the rat osteocalcin basal promoter in osteoblastic cells is dependent upon homeodomain and CP1 binding motifs. Mol. Endocrinol. 8:614–624.
  • Towler, D. A., and J. Rodan 1995. Identification of a rat osteocalcin promoter 3′,5′-cyclic adenosine monophosphate response region containing two PuGGTCA steroid hormone receptor binding motifs. Endocrinology 136:1089–1096.
  • Towler, D. A., S. J. Rutledge, and J. Rodan 1994. Msx-2/Hox 8.1: a transcriptional regulator of the rat osteocalcin promoter. Mol. Endocrinol. 8:1484–1493.
  • Truss, M., J. Bartsch, C. Mows, S. Chavez, and J. Beato 1996. Chromatin structure of the MMTV promoter and its changes during hormonal induction. Cell Mol. Neurobiol. 16:85–101.
  • Wu, C. 1980. The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature 286:854–860.
  • Xiao, Z. S., R. Thomas, T. K. Hinson, and J. Quarles 1998. Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. Gene 214:187–197.
  • Zaret, K. S., and J. Yamamoto 1984. Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element. Cell 38:29–38.
  • Zeng, C., S. McNeil, S. Pockwinse, J. A. Nickerson, L. Shopland, J. B. Lawrence, S. Penman, S. W. Hiebert, J. B. Lian, A. J. van Wijnen, J. L. Stein, and J. Stein 1998. Intranuclear targeting of AML/CBFα regulatory factors to nuclear matrix-associated transcriptional domains. Proc. Natl. Acad. Sci. USA 95:1585–1589.
  • Zeng, C., A. J. van Wijnen, J. L. Stein, S. Meyers, W. Sun, L. Shopland, J. B. Lawrence, S. Penman, J. B. Lian, G. S. Stein, and J. Hiebert 1997. Identification of a nuclear matrix targeting signal in the leukemia and bone-related AML/CBFα transcription factors. Proc. Natl. Acad. Sci. USA 94:6746–6751.
  • Zhang, R., P. Ducy, and J. Karsenty 1997. 1,25-dihydroxyvitamin D3 inhibits osteocalcin expression in mouse through an indirect mechanism. J. Biol. Chem. 272:110–116.
  • Zhang, Y. W., S. C. Bae, E. Takahashi, and J. Ito 1997. The cDNA cloning of the transcripts of human PEBP2alphaA/CBFA1 mapped to 6p12.3-p21.1, the locus for cleidocranial dysplasia. Oncogene 15:367–371.
  • Zhou, H., S. S. Manji, D. M. Findlay, T. J. Martin, J. K. Heath, and J. Ng 1994. Novel action of retinoic acid. Stabilization of newly synthesized alkaline phosphatase transcripts. J. Biol. Chem. 269:22433–22439.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.