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Gene Expression

Differential and Inefficient Splicing of a Broadly Expressed Drosophila erect wing Transcript Results in Tissue-Specific Enrichment of the Vital EWG Protein Isoform

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Pages 3998-4007 | Received 08 Jan 1999, Accepted 08 Mar 1999, Published online: 27 Mar 2023

REFERENCES

  • Adams, M. D., R. S. Tarng, and J. Rio 1997. The alternative splicing factor PSI regulates P-element third intron splicing in vivo. Genes Dev. 11:129–138.
  • Beall, C. J., and J. Hirsh 1984. High levels of intron-containing RNAs are associated with expression of the Drosophila DOPA decarboxylase gene. Mol. Cell. Biol. 4:1669–1674.
  • Becker, T. S., S. M. Burgess, A. H. Amsterdam, M. L. Allende, and J. Hopkins 1998. Not really finished is crucial for development of the zebrafish outer retina and encodes a transcription factor highly homologous to human nuclear respiratory factor-1 and avian initiation binding repressor. Development 125:4369–4378.
  • Calzone, F. J., C. Hoog, D. B. Teplow, A. E. Cutting, R. W. Zeller, R. J. Britten, and J. Davidson 1991. Gene regulatory factors of the sea urchin embryo. I. Purification by affinity chromatography and cloning of P3A2, a novel DNA-binding protein. Development 112:335–350.
  • Chabot, B. 1996. Directing alternative splicing: cast and scenarios. Trends Genet. 12:472–478.
  • Chang, D. D., and J. Sharp 1989. Regulation by HIV Rev depends upon recognition of splice sites. Cell 59:789–795.
  • Cheung, P. H., O. Culic, Y. Qiu, K. Earley, N. Thompson, D. C. Hixson, and J. Lin 1993. The cytoplasmic domain of C-CAM is required for C-CAM-mediated adhesion function: studies of a C-CAM transcript containing an unspliced intron. Biochem. J. 295:427–435.
  • DeSimone, S., C. Coelho, S. Roy, K. VijayRaghavan, and J. White 1996. ERECT WING, the Drosophila member of a family of DNA binding proteins is required in imaginal myoblasts for flight muscle development. Development 121:31–39.
  • DeSimone, S. M. 1992. Ph.D. thesis. Brandeis University, Waltham, Mass.
  • DeSimone, S. M., and J. White 1993. The Drosophila erect wing gene, which is important for both neuronal and muscle development, encodes a protein which is similar to the sea urchin P3A2 DNA binding protein. Mol. Cell. Biol. 13:3641–3649.
  • Driksen, W. P., Q. Sun, and J. Rottman 1995. Multiple splicing signals control alternative intron retention of bovine growth hormone pre-mRNA. J. Biol. Chem. 270:5346–5352.
  • Ebihara, K., Y. Masuhiro, T. Kitamoto, M. Suzawa, Y. Uematsu, T. Yoshizawa, T. Ono, H. Harada, K. Matsuda, T. Hasegawa, S. Masushige, and J. Kato 1996. Intron retention generates a novel isoform of the murine vitamin D receptor that acts in a dominant negative way on the vitamin D signalling pathway. Mol. Cell. Biol. 16:3393–3400.
  • Efiok, B. J. S., J. A. Chiorini, and J. Safer 1994. A key transcription factor for eukaryotic initiation factor-2a is strongly homologous to developmental transcription factors and may link metabolic genes to cellular growth and development. J. Biol. Chem. 269:18921–18930.
  • Fleming, R. J. 1987. Ph.D. thesis. Brandeis University, Waltham, Mass.
  • Fleming, R. J., S. M. DeSimone, and J. White 1989. Molecular isolation and analysis of the erect wing locus in Drosophila melanogaster. Mol. Cell. Biol. 9:719–725.
  • Fleming, R. J., S. Zusman, and J. White 1983. Developmental genetic analysis of lethal alleles at the ewg locus and their effects on muscle development in Drosophila melanogaster. Dev. Genet. 3:347–363.
  • Ghosh, S., and J. Lowenstein 1996. A multifunctional vector system for heterologous expression of proteins in Escherichia coli: expression of native and hexahistidyl fusion proteins, rapid purification of the fusion proteins, and removal of fusion peptide by Kex2 protease. Gene 176:249–255.
  • Gomez-Cuadrado, A., M. Martin, M. Noel, and J. Ruiz-Carrillo 1995. Initiation binding receptor, a factor that binds to the transcription initiation site of the histone h5 gene, is a glycosylated member of a family of cell growth regulators. Mol. Cell. Biol. 15:6670–6685.
  • Grabowski, P. 1998. Splicing regulation in neurons: tinkering with cell-specific control. Cell 92:709–712.
  • Gugneja, S., C. A. Virbasius, and J. Scarpulla 1996. Nuclear respiratory factors 1 and 2 utilize similar glutamine-containing clusters of hydrophobic residues to activate transcription. Mol. Cell. Biol. 16:5708–5716.
  • Hamm, J., and J. Mattaj 1990. Monomethylated cap structures facilitate RNA export from the nucleus. Cell 63:109–118.
  • Horabin, J. I., and J. Schedl 1993. Regulated splicing of the Drosophila Sex-lethal male exon involves a blockage mechanism. Mol. Cell. Biol. 13:1408–1414.
  • Legrain, P., and J. Rosbash 1989. Some cis-acting and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Cell 57:573–583.
  • López, A. J. 1995. Developmental role of transcription factor isoforms generated by alternative splicing. Dev. Biol. 172:396–411.
  • Mattox, W., and J. Baker 1991. Autoregulation of the splicing of transcripts from the transformer-2 gene of Drosophila. Genes Dev. 5:786–796.
  • Mount, S. M., C. Burks, G. Hertz, G. D. Stormo, O. White, and J. Fields 1992. Splicing signals in Drosophila: intron size, information content, and consensus sequences. Nucleic Acids Res. 20:4255–4262.
  • Padgett, R. A., P. J. Grabowski, M. M. Konarska, S. Seiler, and J. Sharp 1986. Splicing of messenger RNA precursors. Annu. Rev. Biochem. 55:1119–1150.
  • Rozen, S., and H. Skaletsky. 1996, posting date. Primer3. [Online.] http://www.genome.wi.mit.edu/genome_software/other/primer3.html [7 April 1999, last date accessed.]
  • Samuels, M. E., P. Schedl, and J. Cline 1991. The complex set of late transcripts from the Drosophila sex determination gene Sex-lethal encodes multiple related polypeptides. Mol. Cell. Biol. 11:3584–3602.
  • Talerico, M., and J. Berget 1994. Intron definition in splicing of small Drosophila introns. Mol. Cell. Biol. 14:3434–3445.
  • Torroja, L., L. Luo, and J. White 1996. APPL, the Drosophila member of the APP-family, exhibits differential trafficking and processing in CNS neurons. J. Neurosci. 16:4638–4650.
  • Virbasius, C. A., J. V. Virbasius, and J. Scarpulla 1993. NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators. Genes Dev. 7:2431–2445.
  • Wang, J., and J. Manley 1997. Regulation of pre-mRNA splicing in metazoa. Curr. Opin. Genet. Dev. 7:205–211.
  • Wang, J., L. Shen, H. Najafi, J. Kolberg, F. M. Matschinsky, M. Urdea, and J. German 1997. Regulation of insulin preRNA splicing by glucose. Proc. Natl. Acad. Sci. USA 94:4360–4365.
  • Zachar, Z., T.-B. Chou, and J. Bingham 1987. Evidence that a regulatory gene autoregulates splicing of its transcript. EMBO 6:4105–4111.
  • Zhang, P., and J. Spradling 1993. Efficient and dispersed local P element transposition from Drosophila females. Genetics 133:361–373.

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