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Cell Growth and Development

Oncogenic Ras Blocks Anoikis by Activation of a Novel Effector Pathway Independent of Phosphatidylinositol 3-Kinase

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Pages 5488-5499 | Received 12 Jan 2001, Accepted 23 May 2001, Published online: 28 Mar 2023

REFERENCES

  • Barbacid, M.. 1987. ras genes. Annu. Rev. Biochem. 56:779–827.
  • Barradas, M., A. Monjas, M. T. Diaz-Meco, M. Serrano, and J. Moscat. 1999. The downregulation of the pro-apoptotic protein Par-4 is critical for Ras-induced survival and tumor progression. EMBO J. 18:6362–6369.
  • Blay, J., and K. D. Brown. 1984. Characterization of an epithelioid cell line derived from the rat small intestine. Cell Biol. Int. Rep. 8:551–560.
  • Bos, J. L.. 1989. ras oncogenes in human cancer: a review. Cancer Res. 49:4682–4689.
  • Boucher, M. J., J. Morisset, P. H. Vachon, J. C. Reed, J. Laine, and N. Rivard. 2000. MEK/ERK signaling pathway regulates the expression of bcl-2, bcl-X(L), and mcl-1 and promotes survival of human pancreatic cancer cells. J. Cell. Biochem. 79:355–369.
  • Boudreau, N., C. J. Sympson, Z. Werb, and M. J. Bissell. 1995. Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science 267:891–893.
  • Campbell, S. L., R. Khosravi-Far, K. L. Rossman, G. J. Clark, and C. J. Der. 1998. Increasing complexity of Ras signaling. Oncogene 17:1395–1413.
  • Cardone, M. H., G. S. Salvesen, C. Widmann, G. Johnson, and S. M. Frisch. 1997. The regulation of anoikis: MEKK-1 activation requires cleavage by caspases. Cell 90:315–323.
  • Clark, G. J., A. D. Cox, S. M. Graham, and C. J. Der. 1995. Biological assays for Ras transformation. Methods Enzymol. 255:395–412.
  • Clark, G. J., and C. J. Der. 1993. Oncogenic activation of Ras proteins. GTPases in biology I.. B. F. Dickey, and L. Birnbaumer. 259–288. Springer Verlag, Berlin, Germany
  • Cox, A. D., T. R. Brtva, D. G. Lowe, and C. J. Der. 1994. R-Ras induces malignant, but not morphologic, transformation of NIH3T3 cells. Oncogene 9:3281–3288.
  • Downward, J.. 1998. Mechanisms and consequences of activation of protein kinase B/Akt. Curr. Opin. Cell Biol. 10:262–267.
  • Favata, M. F., K. Y. Horiuchi, E. J. Manos, A. J. Daulerio, D. A. Stradley, W. S. Feeser, D. E. Van Dyk, W. J. Pitts, R. A. Earl, F. Hobbs, R. A. Copeland, R. L. Magolda, P. A. Scherle, and J. M. Trzaskos. 1998. Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J. Biol. Chem. 273:18623–18632.
  • Fearnhead, H. O., D. Dinsdale, and G. M. Cohen. 1995. An interleukin-1 beta-converting enzyme-like protease is a common mediator of apoptosis in thymocytes. FEBS Lett. 375:283–288.
  • Feig, L. A., T. Urano, and S. Cantor. 1996. Evidence for a Ras/Ral signaling cascade. Trends Biochem. Sci. 21:438–441.
  • Franke, T. F., S. I. Yang, T. O. Chan, K. Datta, A. Kazlauskas, D. K. Morrison, D. R. Kaplan, and P. N. Tsichlis. 1995. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81:727–736.
  • Frisch, S. M., and H. Francis. 1994. Disruption of epithelial cell-matrix interactions induces apoptosis. J. Cell Biol. 124:619–626.
  • Frisch, S. M., and E. Ruoslahti. 1997. Integrins and anoikis. Curr. Opin. Cell Biol. 9:701–706.
  • Fry, D. W., A. J. Kraker, A. McMichael, L. A. Ambroso, J. M. Nelson, W. R. Leopold, R. W. Connors, and A. J. Bridges. 1994. A specific inhibitor of the epidermal growth factor receptor tyrosine kinase. Science 265:1093–1095.
  • Gangarosa, L. M., N. Sizemore, R. Graves-Deal, S. M. Oldham, C. J. Der, and R. J. Coffey. 1997. A Raf-independent epidermal growth factor receptor autocrine loop is necessary for Ras transformation of rat intestinal epithelial cells. J. Biol. Chem. 272:18926–18931.
  • Graham, S. M., A. B. Vojtek, S. Y. Huff, A. D. Cox, G. J. Clark, J. A. Cooper, and C. J. Der. 1996. TC21 causes transformation by Raf-independent signaling pathways. Mol. Cell. Biol. 16:6132–6140.
  • Hahn, W. C., C. M. Counter, A. S. Lundberg, R. L. Beijersbergen, M. W. Brooks, and R. A. Weinberg. 1999. Creation of human tumour cells with defined genetic elements. Nature 400:464–468.
  • Hall, P. A., P. J. Coates, B. Ansari, and D. Hopwood. 1994. Regulation of cell number in the mammalian gastrointestinal tract: the importance of apoptosis. J. Cell Sci. 107:3569–3577.
  • Han, L., D. Wong, A. Dhaka, D. Afar, M. White, W. Xie, H. Herschman, O. Witte, and J. Colicelli. 1997. Protein binding and signaling properties of RIN1 suggest a unique effector function. Proc. Natl. Acad. Sci. USA 94:4954–4959.
  • Holmstrom, T. H., S. C. Chow, I. Elo, E. T. Coffey, S. Orrenius, L. Sistonen, and J. E. Eriksson. 1998. Suppression of Fas/APO-1-mediated apoptosis by mitogen-activated kinase signaling. J. Immunol. 160:2626–2636.
  • Huff, S. Y., L. A. Quilliam, A. D. Cox, and C. J. Der. 1997. R-Ras is regulated by activators and effectors distinct from those that control Ras function. Oncogene 14:133–143.
  • Khosravi-Far, R., P. A. Solski, G. J. Clark, M. S. Kinch, and C. J. Der. 1995. Activation of Rac1, RhoA, and mitogen-activated protein kinases is required for Ras transformation. Mol. Cell. Biol. 15:6443–6453.
  • Khosravi-Far, R., M. A. White, J. K. Westwick, P. A. Solski, M. Chrzanowska-Wodnicka, L. Van Aelst, M. H. Wigler, and C. J. Der. 1996. Oncogenic Ras activation of Raf/mitogen-activated protein kinase-independent pathways is sufficient to cause tumorigenic transformation. Mol. Cell. Biol. 16:3923–3933.
  • Khwaja, A., P. Rodriguez-Viciana, S. Wennström, P. H. Warne, and J. Downward. 1997. Matrix adhesion and Ras transformation both activate a phosphoinositide 3-OH kinase and protein kinase B/Akt cellular survival pathway. EMBO J. 16:2783–2793.
  • Kops, G. J., N. D. de Ruiter, A. M. de Vries-Smits, D. R. Powell, J. L. Bos, and B. M. Burgering. 1999. Direct control of the Forkhead transcription factor AFX by protein kinase B. Nature 398:630–634.
  • Leevers, S. J., H. F. Paterson, and C. J. Marshall. 1994. Requirement for Ras in Raf activation is overcome by targeting Raf to the plasma membrane. Nature 369:411–414.
  • Le Gall, M., J. C. Chambard, J. P. Breittmayer, D. Grall, J. Pouyssegur, and E. Obberghen-Schilling. 2000. The p42/p44 MAP kinase pathway prevents apoptosis induced by anchorage and serum removal. Mol. Biol. Cell 11:1103–1112.
  • Marshall, C. J.. 1996. Ras effectors. Curr. Opin. Cell Biol. 8:197–204.
  • Marte, B. M., P. Rodriguez-Viciana, S. Wennström, P. H. Warne, and J. Downward. 1996. R-Ras can activate the phosphoinositide 3-kinase but not the MAP kinase arm of the Ras effector pathways. Curr. Biol. 7:63–70.
  • Oldham, S. M., G. J. Clark, L. M. Gangarosa, R. J. Coffey Jr., and C. J. Der. 1996. Activation of the Raf-1/MAP kinase cascade is not sufficient for Ras transformation of RIE-1 epithelial cells. Proc. Natl. Acad. Sci. USA 93:6924–6928.
  • Oldham, S. M., A. D. Cox, E. R. Reynolds, N. S. Sizemore, R. J. Coffey Jr., and C. J. Der. 1998. Ras, but not Src, transformation of RIE-1 epithelial cells is dependent on activation of the mitogen-activated protein kinase cascade. Oncogene 16:2565–2573.
  • Pear, W. S., G. P. Nolan, M. L. Scott, and D. Baltimore. 1993. Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. USA 90:8392–8396.
  • Perona, R., S. Montaner, L. Saniger, I. Sánchez-Pérez, R. Bravo, and J. C. Lacal. 1997. Activation of the nuclear factor-KB by Rho, CDC42, and Rac-1 proteins. Genes Dev. 11:463–475.
  • Petersen, O. W., L. Ronnov-Jessen, V. M. Weaver, and M. J. Bissell. 1998. Differentiation and cancer in the mammary gland: shedding light on an old dichotomy. Adv. Cancer Res. 75:135–161.
  • Polakowska, R. R., M. Piacentini, R. Bartlett, L. A. Goldsmith, and A. R. Haake. 1994. Apoptosis in human skin development: morphogenesis, periderm, and stem cells. Dev. Dyn. 199:176–188.
  • Ramocki, M. B., M. A. White, S. F. Konieczny, and E. J. Taparowsky. 1998. A role for RalGDS and a novel Ras effector in the Ras-mediated inhibition of skeletal myogenesis. J. Biol. Chem. 273:17696–17701.
  • Rodriguez-Viciana, P., P. H. Warne, R. Dhand, B. Vanhaesebroeck, I. Gout, M. J. Fry, M. D. Waterfield, and J. Downward. 1994. Phosphatidylinositol-3-OH kinase as a direct target of Ras. Nature 370:527–532.
  • Rodriguez-Viciana, P., P. H. Warne, A. Khwaja, B. M. Marte, D. Pappin, P. Das, M. D. Waterfield, A. Ridley, and J. Downward. 1997. Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras. Cell 89:457–467.
  • Rodriguez-Viciana, P., P. H. Warne, B. Vanhaesebroeck, M. D. Waterfield, and J. Downward. 1996. Activation of phosphoinositide 3-kinase by interaction with Ras and by point mutation. EMBO J. 15:2442–2451.
  • Romashkova, J. A., and S. S. Makarov. 1999. NF-kappaB is a target of AKT in anti-apoptotic PDGF signalling. Nature 401:86–90.
  • Rosen, K., J. Rak, J. Jin, R. S. Kerbel, M. J. Newman, and J. Filmus. 1998. Downregulation of the pro-apoptotic protein Bak is required for the ras-induced transformation of intestinal epithelial cells. Curr. Biol. 8:1331–1334.
  • Rosen, K., J. Rak, T. Leung, N. M. Dean, R. S. Kerbel, and J. Filmus. 2000. Activated Ras prevents downregulation of Bcl-X(L) triggered by detachment from the extracellular matrix. A mechanism of Ras-induced resistance to anoikis in intestinal epithelial cells. J. Cell Biol. 149:447–456.
  • Scheid, M. P., K. M. Schubert, and V. Duronio. 1999. Regulation of bad phosphorylation and association with Bcl-x(L) by the MAPK/Erk kinase. J. Biol. Chem. 274:31108–31113.
  • Schwartz, M. A.. 1997. Integrins, oncogenes, and anchorage independence. J. Cell Biol. 139:575–578.
  • Serrano, M., A. W. Lin, M. E. McCurrach, D. Beach, and S. W. Lowe. 1997. Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell 88:593–602.
  • Shields, J. M., K. Pruitt, A. McFall, A. Shaub, and C. J. Der. 2000. Understanding Ras: “it ain't over 'til it's over.”. Trends Cell Biol. 10:147–153.
  • Stanton, V. P. Jr., D. W. Nichols, A. P. Laudano, and G. M. Cooper. 1989. Definition of the human raf amino-terminal regulatory region by deletion mutagenesis. Mol. Cell. Biol. 9:639–647.
  • Sulciner, D. J., K. Irani, Z.-X. Yu, V. J. Ferrans, P. Goldschmidt-Clermont, and T. Finkel. 1996. rac1 regulates a cytokine-stimulated, redox-dependent pathway necessary for NF-κB activation. Mol. Cell. Biol. 16:7115–7121.
  • Urano, T., R. Emkey, and L. A. Feig. 1996. Ral-GTPases mediate a distinct downstream signaling pathway from Ras that facilitates cellular transformation. EMBO J. 15:810–816.
  • Vlahos, C. J., W. F. Matter, K. Y. Hui, and R. F. Brown. 1994. A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J. Biol. Chem. 269:5241–5248.
  • White, M. A., C. Nicolette, A. Minden, A. Polverino, L. Van Aelst, M. Karin, and M. H. Wigler. 1995. Multiple Ras functions can contribute to mammalian cell transformation. Cell 80:533–541.
  • Wolthuis, R. M., and J. L. Bos. 1999. Ras caught in another affair: the exchange factors for Ral. Curr. Opin. Genet. Dev. 9:112–117.
  • Wolthuis, R. M., N. D. de Ruiter, R. H. Cool, and J. L. Bos. 1997. Stimulation of gene induction and cell growth by the Ras effector Rlf. EMBO J. 16:6748–6761.
  • Yan, J., S. Roy, A. Apolloni, A. Lane, and J. F. Hancock. 1998. Ras isoforms vary in their ability to activate Raf-1 and phosphoinositide 3-kinase. J. Biol. Chem. 273:24052–24056.

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