78
Views
139
CrossRef citations to date
0
Altmetric
Cell and Organelle Structure and Assembly

Protein Import Channel of the Outer Mitochondrial Membrane: a Highly Stable Tom40-Tom22 Core Structure Differentially Interacts with Preproteins, Small Tom Proteins, and Import Receptors

, , , , , , , , & show all
Pages 2337-2348 | Received 15 Nov 2000, Accepted 03 Jan 2001, Published online: 27 Mar 2023

REFERENCES

  • Ahting, U., C. Thun, R. Hegerl, D. Typke, F. E. Nargang, W. Neupert, and S. Nussberger. 1999. The TOM core complex: the general protein import pore of the outer membrane of mitochondria. J. Cell Biol. 147:959–968.
  • Alconada, A., F. Gärtner, A. Hönlinger, M. Kübrich, and N. Pfanner. 1995. Mitochondrial receptor complex from Neurospora crassa and Saccharomyces cerevisiae. Methods Enzymol. 260:263–286.
  • Alconada, A., M. Kübrich, M. Moczko, A. Hönlinger, and N. Pfanner. 1995. The mitochondrial receptor complex: the small subunit Mom8b/Isp6 supports association of receptors with the general insertion pore and transfer of preproteins. Mol. Cell. Biol. 15:6196–6205.
  • Bauer, M. F., S. Hofmann, W. Neupert, and M. Brunner. 2000. Protein translocation into mitochondria: the role of TIM complexes. Trends Cell Biol. 10:25–31.
  • Blom, J., M. Kübrich, J. Rassow, W. Voos, P. J. T. Dekker, A. C. Maarse, M. Meijer, and N. Pfanner. 1993. The essential yeast protein MIM44 (encoded by MPI1) is involved in an early step of preprotein translocation across the mitochondrial inner membrane. Mol. Cell. Biol. 13:7364–7371.
  • Daum, G., P. C. Böhni, and G. Schatz. 1982. Import of proteins into mitochondria. cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J. Biol. Chem. 257:13028–13033.
  • Dekker, P. J. T., F. Martin, A. C. Maarse, U. Bömer, H. Müller, B. Guiard, M. Meijer, J. Rassow, and N. Pfanner. 1997. The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44. EMBO J. 16:5408–5419.
  • Dekker, P. J. T., H. Müller, J. Rassow, and N. Pfanner. 1996. Characterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis. Biol. Chem. 77:535–538.
  • Dekker, P. J. T., M. T. Ryan, J. Brix, H. Müller, A. Hönlinger, and N. Pfanner. 1998. Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol. Cell. Biol. 18:6515–6524.
  • Dietmeier, K., A. Hönlinger, U. Bömer, P. J. T. Dekker, C. Eckerskorn, F. Lottspeich, M. Kübrich, and N. Pfanner. 1997. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature 388:195–200.
  • Eilers, M., and G. Schatz. 1986. Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria. Nature 322:228–232.
  • Fujiki, Y., A. L. Hubbard, S. Fowler, and P. B. Lazarow. 1982. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J. Cell Biol. 93:97–102.
  • Gambill, B. D., W. Voos, P. J. Kang, B. Miao, T. Langer, E. A. Craig, and N. Pfanner. 1993. A dual role for mitochondrial heat shock protein 70 in membrane translocation of preproteins. J. Cell Biol. 123:109–117.
  • Glick, B. S.. 1995. Can Hsp70 proteins act as force-generating motors?. Cell 80:11–14.
  • Haucke, V., C. S. Ocana, A. Hönlinger, K. Tokatlidis, N. Pfanner, and G. Schatz. 1997. Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria. Mol. Cell. Biol. 17:4024–4032.
  • Hill, K., K. Model, M. T. Ryan, K. Dietmeier, F. Martin, R. Wagner, and N. Pfanner. 1998. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature 395:516–521.
  • Hines, V., A. Brandt, G. Griffiths, H. Horstmann, H. Brütsch, and G. Schatz. 1990. Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70. EMBO J. 9:3191–3200.
  • Hinnah, S. C., K. Hill, R. Wagner, T. Schlicher, and J. Soll. 1997. Reconstitution of a chloroplast protein import channel. EMBO J. 16:7351–7360.
  • Hönlinger, A., U. Bömer, A. Alconada, C. Eckerskorn, F. Lottspeich, K. Dietmeier, and N. Pfanner. 1996. Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway-related role in protein import. EMBO J. 15:2125–2137.
  • Huang, S., K. S. Ratliff, M. P. Schwartz, J. M. Spenner, and A. Matouschek. 1999. Mitochondria unfold precursor proteins by unraveling them from their N-termini. Nat. Struct. Biol. 6:1132–1138.
  • Jänsch, L., V. Kruft, U. K. Schmitz, and H. P. Braun. 1998. Unique composition of the preprotein translocase of the outer mitochondrial membrane from plants. J. Biol. Chem. 273:17251–17257.
  • Jensen, R. E., and A. E. Johnson. 1999. Protein translocation: is Hsp70 pulling my chain?. Curr. Biol. 9:R779–R782.
  • Kang, P. J., J. Ostermann, J. Shilling, W. Neupert, E. A. Craig, and N. Pfanner. 1990. Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. Nature 348:137–143.
  • Kiebler, M., R. Pfaller, T. Söllner, G. Griffiths, H. Horstmann, N. Pfanner, and W. Neupert. 1990. Identification of a mitochondrial receptor complex required for recognition and membrane insertion of precursor proteins. Nature 348:610–616.
  • Koehler, C. M., K. Jarosch, K. Tokatlidis, K. Schmid, R. J. Schweyen, and G. Schatz. 1998. Import of mitochondrial carriers mediated by essential proteins of the intermembrane space. Science 279:369–373.
  • Koehler, C. M., S. Merchant, and G. Schatz. 1999. How membrane proteins travel across the mitochondrial intermembrane space. Trends Biochem. Sci. 24:428–432.
  • Künkele, K. P., S. Heins, M. Dembowski, F. E. Nargang, R. Benz, M. Thieffry, J. Walz, R. Lill, S. Nussberger, and W. Neupert. 1998. The preprotein translocation channel of the outer membrane of mitochondria. Cell 93:1009–1019.
  • Künkele, K. P., P. Juin, C. Pompa, F. E. Nargang, J. P. Henry, W. Neupert, R. Lill, and M. Thieffry. 1998. The isolated complex of the translocase of the outer membrane of mitochondria: characterization of the cation-selective and voltage-gated preprotein-conducting pore. J. Biol. Chem. 273:31032–31039.
  • Kurz, M., H. Martin, J. Rassow, N. Pfanner, and M. T. Ryan. 1999. Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing-over with the main import pathway. Mol. Biol. Cell 10:2461–2474.
  • Mayer, A., R. Lill, and W. Neupert. 1993. Translocation and insertion of precursor proteins into isolated outer membranes of mitochondria. J. Cell Biol. 121:1233–1243.
  • Mayer, A., W. Neupert, and R. Lill. 1995. Mitochondrial protein import: reversible binding of the presequence at the trans side of the outer membrane drives partial translocation and unfolding. Cell 80:127–137.
  • Meisinger, C., J. Brix, K. Model, N. Pfanner, and M. T. Ryan. 1999. The preprotein translocase of the outer mitochondrial membrane: receptors and a general import pore. Cell. Mol. Life Sci. 56:817–824.
  • Moczko, M., K. Dietmeier, T. Söllner, B. Segui, H. F. Steger, W. Neupert, and N. Pfanner. 1992. Identification of the mitochondrial receptor complex in Saccharomyces cerevisiae. FEBS Lett. 310:265–268.
  • Moczko, M., B. Ehmann, F. Gärtner, A. Hönlinger, E. Schäfer, and N. Pfanner. 1994. Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria. J. Biol. Chem. 269:9045–9051.
  • Neupert, W.. 1997. Protein import into mitochondria. Annu. Rev. Biochem. 66:863–917.
  • Neupert, W., F. U. Hartl, E. A. Craig, and N. Pfanner. 1990. How do polypeptides cross the mitochondrial membranes?. Cell 63:447–450.
  • Pfanner, N., E. A. Craig, and A. Hönlinger. 1997. Mitochondrial preprotein translocase. Annu. Rev. Cell Dev. Biol. 13:25–51.
  • Philippsen, P., A. Stotz, and C. Scherf. 1991. DNA of Saccharomyces cerevisiae. Methods Enzymol. 194:169–182.
  • Ramage, L., T. Junne, K. Hahne, T. Lithgow, and G. Schatz. 1993. Functional cooperation of mitochondrial protein import receptors in yeast. EMBO J. 12:4115–4123.
  • Rapaport, D., K. P. Künkele, M. Dembowski, U. Ahting, F. E. Nargang, W. Neupert, and R. Lill. 1998. Dynamics of the TOM complex of mitochondria during binding and translocation of preproteins. Mol. Cell. Biol. 18:5256–5262.
  • Rassow, J., B. Guiard, U. Wienhues, V. Herzog, F. U. Hartl, and W. Neupert. 1989. Translocation arrest by reversible folding of a precursor protein imported into mitochondria. A means to quantitate translocation contact sites. J. Cell Biol. 109:1421–1428.
  • Ryan, M. T., H. Müller, and N. Pfanner. 1999. Functional staging of ADP/ATP carrier translocation across the outer mitochondrial membrane. J. Biol. Chem. 29:20619–20627.
  • Schägger, H., and G. von Jagow. 1991. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199:223–231.
  • Schatz, G.. 1997. Just follow the acid chain. Nature 388:121–122.
  • Schatz, G., and B. Dobberstein. 1996. Common principles of protein translocation across membranes. Science 271:1519–1526.
  • Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19–27.
  • Sirrenberg, C., M. Endres, H. Fölsch, R. A. Stuart, W. Neupert, and M. Brunner. 1998. Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5. Nature 391:912–915.
  • Söllner, T., G. Griffiths, R. Pfaller, N. Pfanner, and W. Neupert. 1989. MOM19, an import receptor for mitochondrial precursor proteins. Cell 59:1061–1070.
  • Söllner, T., R. Pfaller, G. Griffiths, N. Pfanner, and W. Neupert. 1990. A mitochondrial import receptor for the ADP/ATP carrier. Cell 62:107–115.
  • Stan, T., U. Ahting, M. Dembowski, K. P. Künkele, S. Nussberger, W. Neupert, and D. Rapaport. 2000. Recognition of preproteins by the isolated TOM complex of mitochondria. EMBO J. 19:4895–4902.
  • Steger, H. F., T. Söllner, M. Kiebler, K. A. Dietmeier, R. Pfaller, K. S. Trülzsch, M. Tropschug, W. Neupert, and N. Pfanner. 1990. Import of ADP/ATP carrier into mitochondria: two receptors act in parallel. J. Cell Biol. 111:2353–2363.
  • van Wilpe, S., M. T. Ryan, K. Hill, A. C. Maarse, C. Meisinger, J. Brix, P. J. T. Dekker, M. Moczko, R. Wagner, M. Meijer, B. Guiard, A. Hönlinger, and N. Pfanner. 1999. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature 401:485–489.
  • Viitanen, P. V., G. K. Donaldson, G. H. Lorimer, T. H. Lubben, and A. A. Gatenby. 1991. Complex interactions between the chaperonin 60 molecular chaperone and dihydrofolate reductase. Biochemistry 30:9716–9723.
  • Voisine, C., E. A. Craig, N. Zufall, O. von Ahsen, N. Pfanner, and W. Voos. 1999. The protein import motor of mitochondria: unfolding and trapping of preproteins are distinct and separable functions of matrix Hsp70. Cell 97:565–574.
  • von Ahsen, O., J. H. Lim, P. Caspers, F. Martin, H. J. Schönfeld, J. Rassow, and N. Pfanner. 2000. Cyclophilin-promoted folding of mouse dihydrofolate reductase does not include the slow conversion of the late-folding intermediate to the active enzyme. J. Mol. Biol. 297:809–818.
  • Voos, W., H. Martin, T. Krimmer, and N. Pfanner. 1999. Mechanisms of protein translocation into mitochondria. Biochim. Biophys. Acta 1422:235–254.
  • Wach, A., A. Brachat, C. Alberti-Segui, C. Rebischung, and P. Philippsen. 1997. Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast 13:1065–1075.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.