Caveolin-1 Maintains Activated Akt in Prostate CancerCells through Scaffolding Domain Binding Site Interactions with andInhibition of Serine/Threonine Protein Phosphatases PP1 andPP2A

REFERENCES

• Alessi, D. R., F. B. Caudwell, M. Andjelkovic, B. A. Hemmings, and P. Cohen. 1996. Molecular basis for the substrate specificity of protein kinase B: comparison with MAPKAP kinase-1 and p70 S6 kinase. FEBS Lett. 399: 333–338.
   PubMed Web of Science ®Google Scholar
• Blume-Jensen, P., and T. Hunter. 2001. Oncogenic kinase signalling. Nature 411: 355–365.
   PubMed Web of Science ®Google Scholar
• Bonnefoy-Berard, N., Y. C. Liu, M. von Willebrand, A. Sung, C. Elly, T. Mustelin, H. Yoshida, K. Ishizaka, and A. Altman. 1995. Inhibition of phosphatidylinositol 3-kinase activity by association with 14-3-3 proteins in T cells. Proc. Natl. Acad. Sci. USA 92: 10142–10146.
   PubMed Web of Science ®Google Scholar
• Brazil, D. P., and B. A. Hemmings. 2001. Ten years of protein kinase B signalling: a hard Akt to follow. Trends Biochem. Sci. 26: 657–664.
   PubMed Web of Science ®Google Scholar
• Calin, G. A., M. G. di Iasio, E. Caprini, I. Vorechovsky, P. G. Natali, G. Sozzi, C. M. Croce, G. Barbanti-Brodano, G. Russo, and M. Negrini. 2000. Low frequency of alterations of the alpha (PPP2R1A) and beta (PPP2R1B) isoforms of the subunit A of the serine-threonine phosphatase 2A in human neoplasms. Oncogene 19: 1191–1195.
   PubMed Web of Science ®Google Scholar
• Cantley, L. C., and B. G. Neel. 1999. New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway. Proc. Natl. Acad. Sci. USA 96: 4240–4245.
   PubMed Web of Science ®Google Scholar
• Cohen, P. T. 2002. Protein phosphatase 1—targeted in many directions. J. Cell Sci. 115: 241–256.
   PubMed Web of Science ®Google Scholar
• Couet, J., S. Li, T. Okamoto, T. Ikezu, and M. P. Lisanti. 1997. Identification of peptide and protein ligands for the caveolin-scaffolding domain. Implications for the interaction of caveolin with caveolae-associated proteins. J. Biol. Chem. 272: 6525–6533.
   PubMed Web of Science ®Google Scholar
• Davidson, B., J. M. Nesland, I. Goldberg, J. Kopolovic, W. H. Gotlieb, M. Bryne, G. Ben-Baruch, A. Berner, and R. Reich. 2001. Caveolin-1 expression in advanced-stage ovarian carcinoma—a clinicopathologic study. Gynecol. Oncol. 81: 166–171.
   PubMed Web of Science ®Google Scholar
• Degeorges, A., F. Hoffschir, O. Cussenot, C. Gauville, A. Le Duc, B. Dutrillaux, and F. Calvo. 1995. Recurrent cytogenetic alterations of prostate carcinoma and amplification of c-myc or epidermal growth factor receptor in subclones of immortalized PNT1 human prostate epithelial cell line. Int. J. Cancer 62: 724–731.
   PubMed Web of Science ®Google Scholar
• Deichmann, M., M. Polychronidis, J. Wacker, M. Thome, and H. Naher. 2001. The protein phosphatase 2A subunit Bγ gene is identified to be differentially expressed in malignant melanomas by subtractive suppression hybridization. Melanoma Res. 11: 577–585.
   PubMedGoogle Scholar
• Di Cristofano, A., and P. P. Pandolfi. 2000. The multiple roles of PTEN in tumor suppression. Cell 100: 387–390.
   PubMed Web of Science ®Google Scholar
• Dijkers, P. F., R. H. Medema, C. Pals, L. Banerji, N. S. Thomas, E. W. Lam, B. M. Burgering, J. A. Raaijmakers, J. W. Lammers, L. Koenderman, and P. J. Coffer. 2000. Forkhead transcription factor FKHR-L1 modulates cytokine-dependent transcriptional regulation of p27KIP1. Mol. Cell. Biol. 20: 9138–9148.
   PubMed Web of Science ®Google Scholar
• Engelman, J. A., C. C. Wykoff, S. Yasuhara, K. S. Song, T. Okamoto, and M. P. Lisanti. 1997. Recombinant expression of caveolin-1 in oncogenically transformed cells abrogates anchorage-independent growth. J. Biol. Chem. 272: 16374–16381.
   PubMed Web of Science ®Google Scholar
• Erhardt, P., E. J. Schremser, and G. M. Cooper. 1999. B-Raf inhibits programmed cell death downstream of cytochrome c release from mitochondria by activating the MEK/Erk pathway. Mol. Cell. Biol. 19: 5308–5315.
   PubMed Web of Science ®Google Scholar
• Fine, S. W., M. P. Lisanti, F. Galbiati, and M. Li. 2001. Elevated expression of caveolin-1 in adenocarcinoma of the colon. Am. J. Clin. Pathol. 115: 719–724.
   PubMed Web of Science ®Google Scholar
• Galbiati, F., D. Volonte, J. Liu, F. Capozza, P. G. Frank, L. Zhu, R. G. Pestell, and M. P. Lisanti. 2001. Caveolin-1 expression negatively regulates cell cycle progression by inducing G0/G1 arrest via a p53/p21WAF1/Cip1-dependent mechanism. Mol. Biol. Cell 12: 2229–2244.
   PubMed Web of Science ®Google Scholar
• Gelmann, E. P. 2002. Molecular biology of the androgen receptor. J. Clin. Oncol. 20: 3001–3015.
   PubMed Web of Science ®Google Scholar
• Gioeli, D., S. B. Ficarro, J. J. Kwiek, D. Aaronson, M. Hancock, A. D. Catling, F. M. White, R. E. Christian, R. E. Settlage, J. Shabanowitz, D. F. Hunt, and M. J. Weber. 2002. Androgen receptor phosphorylation. Regulation and identification of the phosphorylation sites. J. Biol. Chem. 277: 29304–29314.
   PubMed Web of Science ®Google Scholar
• Goh, M., F. Chen, M. T. Paulsen, A. M. Yeager, E. S. Dyer, and M. Ljungman. 2001. Phenylbutyrate attenuates the expression of Bcl-XL, DNA-PK, caveolin-1, and VEGF in prostate cancer cells. Neoplasia 3: 331–338.
   PubMed Web of Science ®Google Scholar
• Gounari, F., S. Signoretti, R. Bronson, L. Klein, W. R. Sellers, J. Kum, A. Siermann, M. M. Taketo, H. von Boehmer, and K. Khazaie. 2002. Stabilization of beta-catenin induces lesions reminiscent of prostatic intraepithelial neoplasia, but terminal squamous transdifferentiation of other secretory epithelia. Oncogene 21: 4099–4107.
   PubMedGoogle Scholar
• Graff, J. R., B. W. Konicek, A. M. McNulty, Z. Wang, K. Houck, S. Allen, J. D. Paul, A. Hbaiu, R. G. Goode, G. E. Sandusky, R. L. Vessella, and B. L. Neubauer. 2000. Increased AKT activity contributes to prostate cancer progression by dramatically accelerating prostate tumor growth and diminishing p27Kip1 expression. J. Biol. Chem. 275: 24500–24505.
   PubMed Web of Science ®Google Scholar
• Grimes, C. A., and R. S. Jope. 2001. The multifaceted roles of glycogen synthase kinase 3β in cellular signaling. Prog. Neurobiol. 65: 391–426.
   PubMed Web of Science ®Google Scholar
• Harris, J., D. Werling, J. C. Hope, G. Taylor, and C. J. Howard. 2002. Caveolae and caveolin in immune cells: distribution and functions. Trends Immunol. 23: 158–164.
   PubMed Web of Science ®Google Scholar
• Ho, C. C., P. H. Huang, H. Y. Huang, Y. H. Chen, P. C. Yang, and S. M. Hsu. 2002. Up-regulated caveolin-1 accentuates the metastasis capability of lung adenocarcinoma by inducing filopodia formation. Am. J. Pathol. 161: 1647–1656.
   PubMed Web of Science ®Google Scholar
• Hu, Y. C., K. Y. Lam, S. Law, J. Wong, and G. Srivastava. 2001. Profiling of differentially expressed cancer-related genes in esophageal squamous cell carcinoma (ESCC) using human cancer cDNA arrays: overexpression of oncogene MET correlates with tumor differentiation in ESCC. Clin Cancer Res. 7: 3519–3525.
   PubMed Web of Science ®Google Scholar
• Ikonen, E., and R. G. Parton. 2000. Caveolins and cellular cholesterol balance. Traffic 1: 212–217.
   PubMed Web of Science ®Google Scholar
• Ito, Y., H. Yoshida, K. Nakano, K. Kobayashi, T. Yokozawa, K. Hirai, F. Matsuzuka, N. Matsuura, K. Kakudo, K. Kuma, and A. Miyauchi. 2002. Caveolin-1 overexpression is an early event in the progression of papillary carcinoma of the thyroid. Br. J. Cancer 86: 912–916.
   PubMedGoogle Scholar
• Ivaska, J., L. Nissinen, N. Immonen, J. E. Eriksson, V. M. Kahari, and J. Heino. 2002. Integrinα 2β1 promotes activation of protein phosphatase 2A and dephosphorylation of Akt and glycogen synthase kinase 3β. Mol. Cell. Biol. 22: 1352–1359.
   PubMedGoogle Scholar
• Janssens, V., and J. Goris. 2001. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353: 417–439.
   PubMed Web of Science ®Google Scholar
• Jenkins, R. B., J. Qian, M. M. Lieber, and D. G. Bostwick. 1997. Detection of c-myc oncogene amplification and chromosomal anomalies in metastatic prostatic carcinoma by fluorescence in situ hybridization. Cancer Res. 57: 524–531.
   PubMed Web of Science ®Google Scholar
• Kato, K., Y. Hida, M. Miyamoto, H. Hashida, T. Shinohara, T. Itoh, S. Okushiba, S. Kondo, and H. Katoh. 2002. Overexpression of caveolin-1 in esophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage. Cancer 94: 929–933.
   PubMed Web of Science ®Google Scholar
• Kohno, T., S. Takakura, T. Yamada, A. Okamoto, T. Tanaka, and J. Yokota. 1999. Alterations of the PPP1R3 gene in human cancer. Cancer Res. 59: 4170–4174.
   PubMedGoogle Scholar
• Kuczyk, M. A., C. Bokemeyer, J. Hartmann, J. Schubach, C. Walter, S. Machtens, R. Knuchel, C. Kollmannsberger, U. Jonas, and J. Serth. 2001. Predictive value of altered p27Kip1 and p21WAF/Cip1 protein expression for the clinical prognosis of patients with localized prostate cancer. Oncol. Rep. 8: 1401–1407.
   PubMedGoogle Scholar
• Lee, S. W., C. L. Reimer, P. Oh, D. B. Campbell, and J. E. Schnitzer. 1998. Tumor cell growth inhibition by caveolin re-expression in human breast cancer cells. Oncogene 16: 1391–1397.
   PubMed Web of Science ®Google Scholar
• Leslie, N. R., and C. P. Downes. 2002. PTEN: the down side of PI 3-kinase signalling. Cell. Signal. 14: 285–295.
   PubMed Web of Science ®Google Scholar
• Li, L., G. Yang, S. Ebara, T. Satoh, Y. Nasu, T. L. Timme, C. Ren, J. Wang, S. A. Tahir, and T. C. Thompson. 2001. Caveolin-1 mediates testosterone-stimulated survival/clonal growth and promotes metastatic activities in prostate cancer cells. Cancer Res. 61: 4386–4392.
   PubMed Web of Science ®Google Scholar
• Lin, H. K., S. Yeh, H. Y. Kang, and C. Chang. 2001. Akt suppresses androgen-induced apoptosis by phosphorylating and inhibiting androgen receptor. Proc. Natl. Acad. Sci. USA 98: 7200–7205.
   PubMed Web of Science ®Google Scholar
• Liu, J., P. Lee, F. Galbiati, R. N. Kitsis, and M. P. Lisanti. 2001. Caveolin-1 expression sensitizes fibroblastic and epithelial cells to apoptotic stimulation. Am. J. Physiol. Cell Physiol. 280: C823–C835.
   PubMedGoogle Scholar
• Loda, M. 2000. p27KIP1: androgen regulation and prognostic significance in prostate cancer. Adv. Clin. Pathol. 4: 226–232.
   PubMedGoogle Scholar
• Lu, M. L., M. C. Schneider, Y. Zheng, X. Zhang, and J. P. Richie. 2001. Caveolin-1 interacts with androgen receptor. A positive modulator of androgen receptor mediated transactivation. J. Biol. Chem. 276: 13442–13451.
   PubMed Web of Science ®Google Scholar
• Massimino, M. L., C. Griffoni, E. Spisni, M. Toni, and V. Tomasi. 2002. Involvement of caveolae and caveolae-like domains in signalling, cell survival and angiogenesis. Cell. Signal. 14: 93–98.
   PubMedGoogle Scholar
• Millward, T. A., S. Zolnierowicz, and B. A. Hemmings. 1999. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24: 186–191.
   PubMed Web of Science ®Google Scholar
• Mouraviev, V., L. Li, S. A. Tahir, G. Yang, T. M. Timme, A. Goltsov, C. Ren, T. Satoh, T. M. Wheeler, M. M. Ittmann, B. J. Miles, R. J. Amato, D. Kadmon, and T. C. Thompson. 2002. The role of caveolin-1 in androgen insensitive prostate cancer. J. Urol. 168: 1589–1596.
   PubMedGoogle Scholar
• Mousses, S., U. Wagner, Y. Chen, J. W. Kim, L. Bubendorf, M. Bittner, T. Pretlow, A. G. Elkahloun, J. B. Trepel, and O. P. Kallioniemi. 2001. Failure of hormone therapy in prostate cancer involves systematic restoration of androgen responsive genes and activation of rapamycin sensitive signaling. Oncogene 20: 6718–6723.
   PubMed Web of Science ®Google Scholar
• Murillo, H., H. Huang, L. J. Schmidt, D. I. Smith, and D. J. Tindall. 2001. Role of PI3K signaling in survival and progression of LNCaP prostate cancer cells to the androgen refractory state. Endocrinology 142: 4795–4805.
   PubMed Web of Science ®Google Scholar
• Nakamura, N., S. Ramaswamy, F. Vazquez, S. Signoretti, M. Loda, and W. R. Sellers. 2000. Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Mol. Cell. Biol. 20: 8969–8982.
   PubMed Web of Science ®Google Scholar
• Nasu, Y., T. L. Timme, G. Yang, C. H. Bangma, L. Li, C. Ren, S. H. Park, M. DeLeon, J. Wang, and T. C. Thompson. 1998. Suppression of caveolin expression induces androgen sensitivity in metastatic androgen-insensitive mouse prostate cancer cells. Nat. Med. 4: 1062–1064.
   PubMed Web of Science ®Google Scholar
• Navarro, D., O. P. Luzardo, L. Fernandez, N. Chesa, and B. N. Diaz-Chico. 2002. Transition to androgen-independence in prostate cancer. J. Steroid Biochem. Mol. Biol. 81: 191–201.
   PubMed Web of Science ®Google Scholar
• Nestl, A., O. D. Von Stein, K. Zatloukal, W. G. Thies, P. Herrlich, M. Hofmann, and J. P. Sleeman. 2001. Gene expression patterns associated with the metastatic phenotype in rodent and human tumors. Cancer Res. 61: 1569–1577.
   PubMedGoogle Scholar
• Nicholson, K. M., and N. G. Anderson. 2002. The protein kinase B/Akt signalling pathway in human malignancy. Cell. Signal. 14: 381–395.
   PubMed Web of Science ®Google Scholar
• Parpal, S., M. Karlsson, H. Thorn, and P. Stralfors. 2001. Cholesterol depletion disrupts caveolae and insulin receptor signaling for metabolic control via insulin receptor substrate-1, but not for mitogen-activated protein kinase control. J. Biol. Chem. 276: 9670–9678.
   PubMed Web of Science ®Google Scholar
• Podar, K., Y. T. Tai, C. E. Cole, T. Hideshima, M. Sattler, A. Hamblin, N. Mitsiades, R. L. Schlossman, F. E. Davies, G. J. Morgan, N. C. Munshi, D. Chauhan, and K. C. Anderson. 2003. Essential role of caveolae in interleukin-6- and insulin-like growth factor I-triggered Akt-1-mediated survival of multiple myeloma cells. J. Biol. Chem. 278: 5794–5801.
   PubMed Web of Science ®Google Scholar
• Rajjayabun, P. H., S. Garg, G. C. Durkan, R. Charlton, M. C. Robinson, and J. K. Mellon. 2001. Caveolin-1 expression is associated with high-grade bladder cancer. Urology 58: 811–814.
   PubMed Web of Science ®Google Scholar
• Razani, B., A. Schlegel, J. Liu, and M. P. Lisanti. 2001. Caveolin-1, a putative tumour suppressor gene. Biochem. Soc. Trans. 29: 494–499.
   PubMed Web of Science ®Google Scholar
• Ren, C., L. Li, A. A. Goltsov, T. L. Timme, S. A. Tahir, J. Wang, L. Garza, A. C. Chinault, and T. C. Thompson. 2002. mRTVP-1, a novel p53 target gene with proapoptotic activities. Mol. Cell. Biol. 22: 3345–3357.
   PubMed Web of Science ®Google Scholar
• Ruediger, R., H. T. Pham, and G. Walter. 2001. Alterations in protein phosphatase 2A subunit interaction in human carcinomas of the lung and colon with mutations in the Aβ subunit gene. Oncogene 20: 1892–1899.
   PubMed Web of Science ®Google Scholar
• Ruediger, R., H. T. Pham, and G. Walter. 2001. Disruption of protein phosphatase 2A subunit interaction in human cancers with mutations in the Aα subunit gene. Oncogene 20: 10–15.
   PubMed Web of Science ®Google Scholar
• Schonthal, A. H. 2001. Role of serine/threonine protein phosphatase 2A in cancer. Cancer Lett. 170: 1–13.
   PubMed Web of Science ®Google Scholar
• Schroeder, F., A. M. Gallegos, B. P. Atshaves, S. M. Storey, A. L. McIntosh, A. D. Petrescu, H. Huang, O. Starodub, H. Chao, H. Yang, A. Frolov, and A. B. Kier. 2001. Recent advances in membrane microdomains: rafts, caveolae, and intracellular cholesterol trafficking. Exp. Biol. Med. (Maywood) 226: 873–890.
   PubMed Web of Science ®Google Scholar
• Shack, S., X. T. Wang, G. C. Kokkonen, M. Gorospe, D. L. Longo, and N. J. Holbrook. 2003. Caveolin-induced activation of the phosphatidylinositol 3-kinase/Akt pathway increases arsenite cytotoxicity. Mol. Cell. Biol. 23: 2407–2414.
   PubMedGoogle Scholar
• Shaul, P. W., and R. G. Anderson. 1998. Role of plasmalemmal caveolae in signal transduction. Am. J. Physiol. 275: L843–L851.
   PubMedGoogle Scholar
• Smart, E. J., G. A. Graf, M. A. McNiven, W. C. Sessa, J. A. Engelman, P. E. Scherer, T. Okamoto, and M. P. Lisanti. 1999. Caveolins, liquid-ordered domains, and signal transduction. Mol. Cell. Biol. 19: 7289–7304.
   PubMed Web of Science ®Google Scholar
• Sontag, E. 2001. Protein phosphatase 2A: the Trojan Horse of cellular signaling. Cell. Signal. 13: 7–16.
   PubMed Web of Science ®Google Scholar
• Stahl, M., P. F. Dijkers, G. J. Kops, S. M. Lens, P. J. Coffer, B. M. Burgering, and R. H. Medema. 2002. The forkhead transcription factor FoxO regulates transcription of p27Kip1 and Bim in response to IL-2. J. Immunol. 168: 5024–5031.
   PubMed Web of Science ®Google Scholar
• Sternberg, P. W., and S. L. Schmid. 1999. Caveolin, cholesterol and Ras signalling. Nat. Cell Biol. 1: E35–E37.
   PubMedGoogle Scholar
• Suzuoki, M., M. Miyamoto, K. Kato, K. Hiraoka, T. Oshikiri, Y. Nakakubo, A. Fukunaga, T. Shichinohe, T. Shinohara, T. Itoh, S. Kondo, and H. Katoh. 2002. Impact of caveolin-1 expression on prognosis of pancreatic ductal adenocarcinoma. Br. J. Cancer 87: 1140–1144.
   PubMed Web of Science ®Google Scholar
• Tahir, S. A., C. Ren, T. L. Timme, Y. Gdor, R. Hoogeveen, J. D. Morrisett, A. Frolov, G. Ayala, T. M. Wheeler, and T. C. Thompson. 2003. Development of an immunoassay for serum caveolin-1: a novel biomarker for prostate cancer. Clin Cancer Res. 9: 3653–3659.
   PubMed Web of Science ®Google Scholar
• Tahir, S. A., G. Yang, S. Ebara, T. L. Timme, T. Satoh, L. Li, A. Goltsov, M. Ittmann, J. D. Morrisett, and T. C. Thompson. 2001. Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer. Cancer Res. 61: 3882–3885.
   PubMed Web of Science ®Google Scholar
• Takakura, S., T. Kohno, R. Manda, A. Okamoto, T. Tanaka, and J. Yokota. 2001. Genetic alterations and expression of the protein phosphatase 1 genes in human cancers. Int. J. Oncol. 18: 817–824.
   PubMed Web of Science ®Google Scholar
• Tamrakar, S., E. Rubin, and J. W. Ludlow. 2000. Role of pRB dephosphorylation in cell cycle regulation. Front. Biosci. 5: D121–D137.
   PubMedGoogle Scholar
• Testa, J. R., and A. Bellacosa. 2001. AKT plays a central role in tumorigenesis. Proc. Natl. Acad. Sci. USA 98: 10983–10985.
   PubMed Web of Science ®Google Scholar
• Thompson, T. C., T. L. Timme, L. Li, and A. Goltsov. 1999. Caveolin-1—a metastasis-related gene that promotes cell survival in prostate cancer. Apoptosis 4: 233–237.
   PubMedGoogle Scholar
• Thompson, T. C., T. L. Timme, L. Li, C. Ren, A. Goltsov, S. Tahir, and G. Yang. 2001. Molecular pathways that underlie prostate cancer progression: the role of caveolin-1. In L. Chung (ed.), Prostate cancer in the 21st century. Humana Press, Totowa, N.J.
   Google Scholar
• Thompson, T. C., T. L. Timme, and I. Sehgal. 1998. Oncogenes, growth factors, and hormones in prostate cancer, p. 327–359. In R. B. Dickson and D. S. Salomon (ed.), Hormones and growth factors in development and neoplasia. Wiley-Liss, Inc., New York, N.Y.
   Google Scholar
• Timme, T. L., A. Goltsov, S. Tahir, L. Li, J. Wang, C. Ren, R. N. Johnston, and T. C. Thompson. 2000. Caveolin-1 is regulated by c-myc and suppresses c-myc-induced apoptosis. Oncogene 19: 3256–3265.
   PubMed Web of Science ®Google Scholar
• Tso, C. L., W. H. McBride, J. Sun, B. Patel, K. H. Tsui, S. H. Paik, B. Gitlitz, R. Caliliw, A. van Ophoven, L. Wu, J. deKernion, and A. Belldegrun. 2000. Androgen deprivation induces selective outgrowth of aggressive hormone-refractory prostate cancer clones expressing distinct cellular and molecular properties not present in parental androgen-dependent cancer cells. Cancer J. 6: 220–233.
   PubMed Web of Science ®Google Scholar
• Tsuchiya, N., Y. Kondo, A. Takahashi, H. Pawar, J. Qian, K. Sato, M. M. Lieber, and R. B. Jenkins. 2002. Mapping and gene expression profile of the minimally overrepresented 8q24 region in prostate cancer. Am. J. Pathol. 160: 1799–1806.
   PubMedGoogle Scholar
• Ushio-Fukai, M., L. Hilenski, N. Santanam, P. L. Becker, Y. Ma, K. K. Griendling, and R. W. Alexander. 2001. Cholesterol depletion inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells: role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling. J. Biol. Chem. 276: 48269–48275.
   PubMed Web of Science ®Google Scholar
• Vis, A. N., B. W. van Rhijn, M. A. Noordzij, F. H. Schroder, and T. H. van der Kwast. 2002. Value of tissue markers p27kip1, MIB-1, and CD44s for the pre-operative prediction of tumour features in screen-detected prostate cancer. J. Pathol. 197: 148–154.
   PubMed Web of Science ®Google Scholar
• Vivanco, I., and C. L. Sawyers. 2002. The phosphatidylinositol 3-kinase AKT pathway in human cancer. Nat. Rev. Cancer 2: 489–501.
   PubMed Web of Science ®Google Scholar
• Wang, S. S., E. D. Esplin, J. L. Li, L. Huang, A. Gazdar, J. Minna, and G. A. Evans. 1998. Alterations of the PPP2R1B gene in human lung and colon cancer. Science 282: 284–287.
   PubMed Web of Science ®Google Scholar
• Wen, Y., M. C. Hu, K. Makino, B. Spohn, G. Bartholomeusz, D. H. Yan, and M. C. Hung. 2000. HER-2/neu promotes androgen-independent survival and growth of prostate cancer cells through the Akt pathway. Cancer Res. 60: 6841–6845.
   PubMed Web of Science ®Google Scholar
• Wera, S., and B. A. Hemmings. 1995. Serine/threonine protein phosphatases. Biochem. J. 311: 17–29.
   PubMed Web of Science ®Google Scholar
• Wu, D., T. L. Foreman, C. W. Gregory, M. A. McJilton, G. G. Wescott, O. H. Ford, R. F. Alvey, J. L. Mohler, and D. M. Terrian. 2002. Protein kinase Cε has the potential to advance the recurrence of human prostate cancer. Cancer Res. 62: 2423–2429.
   PubMed Web of Science ®Google Scholar
• Yang, G., L. D. Truong, T. L. Timme, C. Ren, T. M. Wheeler, S. H. Park, Y. Nasu, C. H. Bangma, M. W. Kattan, P. T. Scardino, and T. C. Thompson. 1998. Elevated expression of caveolin is associated with prostate and breast cancer. Clin. Cancer Res. 4: 1873–1880.
   PubMed Web of Science ®Google Scholar
• Yang, G., L. D. Truong, T. M. Wheeler, and T. C. Thompson. 1999. Caveolin-1 expression in clinically confined human prostate cancer: a novel prognostic marker. Cancer Res. 59: 5719–5723.
   PubMed Web of Science ®Google Scholar
• Yang, R. M., J. Naitoh, M. Murphy, H. J. Wang, J. Phillipson, J. B. deKernion, M. Loda, and R. E. Reiter. 1998. Low p27 expression predicts poor disease-free survival in patients with prostate cancer. J. Urol. 159: 941–945.
   PubMed Web of Science ®Google Scholar
• Zundel, W., L. M. Swiersz, and A. Giaccia. 2000. Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide. Mol. Cell. Biol. 20: 1507–1514.
   PubMed Web of Science ®Google Scholar