Severe Global DNA Hypomethylation Blocks Differentiation and Induces Histone Hyperacetylation in Embryonic Stem Cells

REFERENCES

• Abe, K., Niwa H., Iwase K., Takiguchi M., Mori M., Abe S. I., and Yamamura K. I.. 1996. Endoderm-specific gene expression in embryonic stem cells differentiated to embryoid bodies. Exp. Cell Res. 229:27–34.
   PubMed Web of Science ®Google Scholar
• Cameron, E. E., Bachman K. E., Myohanen S., Herman J. G., and Baylin S. B.. 1999. Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer. Nat. Genet. 21:103–107.
   PubMed Web of Science ®Google Scholar
• Carlson, L. L., Page A. W., and Bestor T. H.. 1992. Properties and localisation of DNA methyltransferase in pre-implantation mouse embryos: implications for genomic imprinting. Genes Dev. 6:2536–2541.
   PubMed Web of Science ®Google Scholar
• Chen, T., Ueda Y., Dodge J. E., Wang J., and Li E.. 2003. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol. Cell. Biol. 23:5594–5605.
   PubMed Web of Science ®Google Scholar
• Darnell, J. E., Jr. 1997. STATs and gene regulation. Science 277:1630–1635.
   PubMed Web of Science ®Google Scholar
• Duncan, S. A., Manova K., Chen W. S., Hoodless P., Weinstein D. C., Bachvarova R. F., and Darnell J. E., Jr. 1994. Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst. Proc. Natl. Acad. Sci. USA 91:7598–7602.
   PubMed Web of Science ®Google Scholar
• Fuks, F., Hurd P. J., Wolf D., Nan X., Bird A. P., and Kouzarides T.. 2002. The methyl-CpG-binding protein MeCP2 links DNA methylation to histone methylation. J. Biol. Chem. 278:4035–4040.
   PubMedGoogle Scholar
• Herrmann, B. G., Labeit S., Poustka A., King T. R., and Lehrach H.. 1990. Cloning of the T gene required in mesoderm formation in the mouse. Nature 343:617–622.
   PubMed Web of Science ®Google Scholar
• Jepsen, K., and Rosenfeld M. G.. 2002. Biological roles and mechanistic actions of co-repressor complexes. J. Cell Sci. 115:689–698.
   PubMed Web of Science ®Google Scholar
• Johnson, M. H., and Ziomek C. A.. 1981. The foundation of two distinct cell lineages within the mouse morula. Cell 24:71–80.
   PubMed Web of Science ®Google Scholar
• Lee, J. H., Hart S. R., and Skalnik D. G.. 2004. Histone deacetylase activity is required for embryonic stem cell differentiation. Genesis 38:32–38.
   PubMed Web of Science ®Google Scholar
• Lehnertz, B., Ueda Y., Derijck A. A., Braunschweig U., Perez-Burgos L., Kubicek S., Chen T., Li E., Jenuwein T., and Peters A. H.. 2003. Suv39h-mediated histone h3 lysine-9 methylation directs DNA methylation to major satellite repeats at pericentric heterochromatin. Curr. Biol. 13:1192–1200.
   PubMed Web of Science ®Google Scholar
• Lei, H., Oh S. P., Okano M., Juttermann R., Goss K. A., Jaenisch R., and Li E.. 1996. De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells. Development 122:3195–3205.
   PubMed Web of Science ®Google Scholar
• Martin, G. R., Wiley L. M., and Damjanov I.. 1977. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61:230–244.
   PubMedGoogle Scholar
• Mayer, W., Niveleau A., Walter J., Fundele R., and Haaf T.. 2000. Demethylation of the zygotic paternal genome. Nature 403:501–502.
   PubMed Web of Science ®Google Scholar
• Nan, X., Ng H.-H., Johnson C. A., Laherty C. D., Turner B. M., Eisenman R. N., and Bird A.. 1998. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature 393:386–389.
   PubMed Web of Science ®Google Scholar
• Niwa, H., Burdon T., Chambers I., and Smith A.. 1998. Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev. 12:2048–2060.
   PubMed Web of Science ®Google Scholar
• Niwa, H., Yamamura K., and Miyazaki J.. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193–199.
   PubMed Web of Science ®Google Scholar
• Okano, M., Bell D. W., Haber D. A., and Li E.. 1999. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99:247–257.
   PubMed Web of Science ®Google Scholar
• Panning, B., and Jaenisch R.. 1996. DNA hypomethylation can activate Xist expression and silence X-linked genes. Genes Dev. 10:1991–2002.
   PubMed Web of Science ®Google Scholar
• Pfeifer, G. P., Steigerwald S. D., Hansen R. S., Gartler S. M., and Riggs A. D.. 1990. Polymerase chain reaction-aided genomic sequencing of an X chromosome-linked CpG island: methylation patterns suggest clonal inheritance, CpG site autonomy, and an explanation of activity state stability. Proc. Natl. Acad. Sci. USA 87:8252–8256.
   PubMed Web of Science ®Google Scholar
• Ramsahoye, B. H. 2002. Nearest-neighbor analysis. Methods Mol. Biol. 200:9–15.
   PubMedGoogle Scholar
• Santos, F., Hendrich B., Reik W., and Dean W.. 2002. Dynamic reprogramming of DNA methylation in the early mouse embryo. Dev. Biol. 241:172–182.
   PubMed Web of Science ®Google Scholar
• Smith, A. 1991. Culture and differentiation of embryonic stem cells. J. Tissue Cult. Methods 13:89–94.
   Google Scholar
• Tamaru, H., and Selker E. U.. 2001. A histone H3 methyltransferase controls DNA methylation in Neurospora crassa. Nature 414:277–283.
   PubMed Web of Science ®Google Scholar
• Tanaka, S., Kunath T., Hadjantonakis A. K., Nagy A., and Rossant J.. 1998. Promotion of trophoblast stem cell proliferation by FGF4. Science 282:2072–2075.
   PubMed Web of Science ®Google Scholar
• Tucker, K. L., Beard C., Dausmann J., Jackson-Grusby L., Laird P. W., Lei H., Li E., and Jaenisch R.. 1996. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes. Genes Dev. 10:1008–1020.
   PubMed Web of Science ®Google Scholar
• Weiss, M. J., Keller G., and Orkin S. H.. 1994. Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells. Genes Dev. 8:1184–1197.
   PubMed Web of Science ®Google Scholar
• Wewer, U. M., Tichy D., Damjanov A., Paulsson M., and Damjanov I.. 1987. Distinct antigenic characteristics of murine parietal yolk sac laminin. Dev. Biol. 121:397–407.
   PubMedGoogle Scholar
• Wiles, M. V., and Keller G.. 1991. Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture. Development 111:259–267.
   PubMed Web of Science ®Google Scholar