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Gene Expression

Functional Expression of the Genomic DNA Sequences Encoding Mouse Na,K-ATPase α1 Gene by Cotransfection of Overlapping Genomic DNA Segments

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Pages 6619-6623 | Received 26 Jun 1990, Accepted 20 Sep 1990, Published online: 31 Mar 2023

LITERATURE CITED

  • Baker, R. M., D. M. Brunette, R. Mankovitz, L. H. Thompson, G. F. Whitmore, L. Siminovitch, and J. E. Till. 1974. Ouabain-resistant mutants of mouse and hamster cells in culture. Cell 1:9–21.
  • Bollag, R. J., A. S. Waldman, and R. M. Liskay. 1989. Homologous recombination in mammalian cells. Annu. Rev. Genet. 23:199–225.
  • De Saint Vincent, B. R., and G. M. Wahl. 1983. Homologous recombination in mammalian cells mediates the formation of a functional gene from two overlapping gene fragments. Proc. Natl. Acad. Sci. USA 80:1902–1906.
  • Emanuel, J. R., S. Graw, D. Housman, and R. Levenson. 1989. Identification of a region within the Na,K-ATPase a subunit that contributes to differential ouabain sensitivity. Mol. Cell. Biol. 9:3744–3749.
  • Emanuel, J. R., J. Schulz, X.-M. Zhan, R. B. Kent, D. Housman, L. Cantley, and R. Levenson. 1988. Expression of an ouabain-resistant Na,K-ATPase in CV-1 cells after transfection with a cDNA encoding the rat Na,K-ATPase a 1 subunit. J. Biol. Chem. 263:7726–7733.
  • Fallows, D., R. B. Kent, D. L. Nelson, J. R. Emanuel, R. Levenson, and D. E. Housman. 1987. Chromosome-mediated transfer of the mouse Na,K-ATPase alpha subunit confers ouabain resistance. Mol. Cell. Biol. 7:2985–2987.
  • Feinberg, A., and B. Vogelstein. 1984. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 67:15–28.
  • Glynn, I. M. 1985. The Na+,K+ transporting adenosine triphosphatase, p. 35–114. In A. Martonosi (ed.), The Enzymes of biological membranes, vol. 3, 2nd ed. Plenum Publishing Corp, New York.
  • Herrera, V. L., J. R. Emanuel, N. Ruiz-Opazo, R. Levenson, and B. Nadal-Ginard. 1987. Three differentially expressed Na,K- ATPase a subunit isoforms: structural and functional implications. J. Cell. Biol. 105:1055–1065.
  • Jorgenson, P. L. 1982. Mechanism of the Na+,K+ pump. Protein structure and conformations of the pure (Na+ + K+)-ATPase. Biochim. Biophys. Acta 694:27–68.
  • Kent, R. B., J. R. Emanuel, Y. B. Neriah, R. Levenson, and D. E. Housman. 1987. Ouabain resistance conferred by expression of the cDNA for a murine Na+,K+-ATPase a subunit. Science 237:901–903.
  • Kent, R. B., D. A. Fallows, E. Geissler, T. Glaser, J. R. Emanuel, P. A. Lalley, R. Levenson, and D. L. Housman. 1987. Genes encoding a and β subunits of the Na,K-AtPase are located on three different chromosomes in the mouse. Proc. Natl. Acad. Sci. USA 84:5369–5372.
  • Kozak, C. A., R. E. K. Fournier, L. A. Leinwand, and F. H. Ruddle. 1979. Assignment of the gene governing ouabain resistance to Mus musculus chromosome 3 using human/mouse microcell hybrids. Biochem. Genet. 17:23–34.
  • Lugo, T. G., and R. M. Baker. 1985. Chromosome-mediated transfer of murine alleles for hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and ouabain resistance into human cell lines. Biochem. Genet. 23:1–15.
  • Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Mankovitz, R., M. Buchwald, and R. M. Baker. 1974. Isolation of ouabain-resistant human diploid fibroblasts. Cell 3:221–226.
  • Milbrandt, J. D., J. C. Azizkhan, K. S. Greisen, and J. C. Hamlin. 1983. Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotype rescue. Mol. Cell. Biol. 3:1266–1273.
  • Price, E. M., and J. B. Lingrel. 1988. Structure-function rela tionships in the Na,K-ATPase a subunit: site directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme. Biochemistry 27:8400–8408.
  • Rossier, B. C., K. Geering, and J. P. Krachenbuhl. 1987. Regulation of the sodium pump: how and why? Trends Biochem. Sci. 12:483–487.
  • Ruobo, A., and J. Kyte. 1974. Photoaffinity labeling of the ouabain-binding site on the Na+ + K+-adenosine triphosphatase. Proc. Natl. Acad. Sci. USA 71:2352–2356.
  • Schwartz, A., G. E. Lindenmayer, and J. C. Allen. 1975. The sodium-potassium adenosine triphosphatase: pharmacological, physiological and biochemical aspects. Pharmacol. Rev. 27:3–134.
  • Shapira, G., J. L. Stachelek, A. Letsou, L. K. Soodak, and R. M. Liskay. 1983. Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells. Proc. Natl. Acad. Sci. USA 80:4827–4831.
  • Shull, G. L., J. Greeb, and J. B. Lingrel. 1986. Molecular cloning of three distinct forms of the Na+,K+-ATPase a subunit from rat brain. Biochemistry 25:8125–8132.
  • Shull, M. M., and J. B. Lingrel. 1987. Multiple genes encode the human Na+,K+-ATPase catalytic subunit. Proc. Natl. Acad. Sci. USA 74:4039–4043.
  • Shull, M. M., D. G. Pugh, and J. B. Lingrel. 1990. The human Na,K-ATPase α 1 gene: characterization of the 5′-flanking region and identification of a restriction fragment length polymorphism. Genomics 6:451–460.
  • Small, J., and G. Scangos. 1983. Recombination during gene transfer into mouse cells can restore the function of deleted genes. Science 220:174–176.
  • Subramani, S., and J. Rubnitz. 1985. Recombination events after transient infection and stable integration of DNA into mouse cells. Mol. Cell. Biol. 5:659–666.
  • Sverdlov, E. D., G. S. Monastyrskaya, N. E. Broude, Y. A. Ushkaryov, R. L. Allikmets, A. M. Melkov, Y. A. Smirnov, I. V. Malyshev, I. E. Dulobova, K. E. Petrukhin, A. V. Grishin, N. I. Kijatkin, M. B. Kostina, V. E. Sverdlov, N. N. Modyanov, and Y. A. Ovchnikov. 1987. The family of human Na,K-ATPase genes: no less than five genes and/or pseudogenes related to the a subunit. FEBS Lett. 217:275–278.
  • Wahl, G. M., K. A. Lewis, J. C. Ruiz, B. Rothenberg, J. Zhao, and G. A. Evans. 1987. Cosmid vectors for rapid genomic walking, restriction mapping and gene transfer. Proc. Natl. Acad. Sci. USA 84:2160–2164.

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