1
Views
26
CrossRef citations to date
0
Altmetric
Gene Expression

Assembly of 60S Ribosomal Subunits Is Perturbed in Temperature-Sensitive Yeast Mutants Defective in Ribosomal Protein L16

, &
Pages 5681-5692 | Received 22 May 1991, Accepted 28 Aug 1991, Published online: 31 Mar 2023

References

  • Andrew, C., A. K. Hopper, and B. D. Hall. 1976. A yeast mutant defective in the processing of 27S rRNA precursor. Mol. Gen. Genet. 144:29–37.
  • Auer, J., G. Spicker, and A. Boeck. 1989. Organization and structure of the Methanococcus transcriptional unit homologous to the Escherichia coli "spectinomycin operon." Implications for the evolutionary relationship of 70S and 80S ribosomes. J. Mol. Biol. 209:21–36.
  • Baim, S. B., D. F. Pietras, D. C. Eustice, and F. Sherman. 1985. A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c. Mol. Cell. Biol. 5:1839–1846.
  • Bayliss, F. T., and J. L. Ingraham. 1974. Mutation in Saccharomyces cerevisiae conferring streptomycin and cold sensitivity by affecting ribosome formation and function. J. Bacteriol. 118:319–328.
  • Boeke, J. D., J. Trueheart, G. Natsoulis, and G. R. Fink. 1987. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154:164–175.
  • Bryant, R. E., and P. S. Sypherd. 1974. Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli. J. Bacteriol. 117:1082–1092.
  • Carter, C. J., M. Cannon, and A. Jimenez. 1980. A trichoder-min-resistant mutant of Saccharomyces cerevisiae with an abnormal distribution of native ribosomal subunits. Eur. J. Biochem. 107:173–183.
  • Ceretti, D. P., D. D. Dean, G. R. Davis, D. M. Bedwell, and M. Nomura. 1983. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 11:2599–2616.
  • Cigan, A. M., and T. F. Donahue. 1987. Sequence and structural features associated with translational initiator regions in yeast—a review. Gene 59:1–13.
  • Dabbs, E. R. 1986. Mutant studies on the prokaryotic ribosome, p. 733-748. In B. Hardesty and G. Kramer (ed.), Structure, function and genetics of ribosomes. Springer-Verlag, New York.
  • Davis, R. W., D. Botstein, and J. R. Roth. 1980. Advanced bacterial genetics, p. 203. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387–395.
  • Donahue, T. F., and A. M. Cigan. 1988. Genetic selection for mutations that reduce or abolish ribosomal recognition of the HIS4 translational initiator region. Mol. Cell. Biol. 8:2955–2963.
  • El-Baradi, T. T. A. L., C. A. F. M. Van der Sande, W. H. Mager, H. A. Raue, and R. J. Planta. 1986. The cellular level of yeast ribosomal protein L25 is controlled principally by rapid degradation of excess protein. Curr. Genet. 10:733–739.
  • Fabian, G. R., and A. K. Hopper. 1987. RRP1, a Saccharomyces cerevisiae gene affecting rRNA processing and production of mature ribosomal subunits. J. Bacteriol. 169:1571–1578.
  • Feinberg, B., C. S. McLaughlin, and K. Moldave. 1982. Analysis of temperature-sensitive mutant ts187 of Saccharomyces cerevisiae altered in a component required for initiation of protein synthesis. J. Biol. Chem. 257:10846–10851.
  • Fleming, G., P. Belhumeur, D. Skup, and H. M. Fried. 1989. Functional substitution of mouse ribosomal protein L27' for yeast ribosomal protein L29 in yeast ribosomes. Proc. Natl. Acad. Sci. USA 86:217–221.
  • Foiani, M., A. M. Cigan, C. J. Paddon, S. Harashima, and A. G. Hinnebusch. Submitted for publication.
  • Futcher, B., and J. Carbon. 1986. Toxic effects of excess cloned centromeres. Mol. Cell. Biol. 6:2213–2222.
  • Gritz, L. R., J. A. Mitlin, M. Cannon, B. Littlewood, C. J. Carter, and J. E. Davies. 1982. Ribosome structure, maturation of ribosomal RNA and drug sensitivity in temperature-sensitive mutants of Saccharomyces cerevisiae. Mol. Gen. Genet. 188:384–391.
  • Guthrie, C., H. Nashimoto, and M. Nomura. 1969. Structure and function of E. coli ribosomes. VIII. Cold-sensitive mutants defective in ribosome assembly. Proc. Natl. Acad. Sci. USA 63:384–391.
  • Hanic-Joyce, P. J., R. A. Singer, and G. C. Johnston. 1987. Molecular characterization of the yeast PRT1 gene in which mutations affect translation initiation and the regulation of cell proliferation. J. Biol. Chem. 262:2845–2851.
  • Haralson, M. A., and D. J. Roufa. 1975. A temperature-sensitive mutation affecting the mammalian 60S ribosome. J. Biol. Chem. 250:4328–4341.
  • Hartwell, L. H., R. K. Mortimer, J. Culotti, and M. Culotti. 1973. Genetic control of the cell division cycle in yeast: V. Genetic analysis of cdc mutants. Genetics 74:267–286.
  • Helser, T. L., R. A. Baan, and A. E. Dahlberg. 1981. Characterization of a 40S ribosomal subunit complex in polyribosomes of Saccharomyces cerevisiae treated with cycloheximide. Mol. Cell. Biol. 1:51–57.
  • Henkin, T. M., S. H. Moon, L. C. Mattheakis, and M. Nomura. 1989. Cloning and analysis of the spc ribosomal protein operon of Bacillus subtilis. Comparison with the spc operon of Escherichia coli. Nucleic Acids Res. 17:7469–7486.
  • Hinnebusch, A. G. 1988. Mechanisms of gene regulation in the general control of amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol. Rev. 52:248–273.
  • Hoffman, C. S., and F. Winston. 1987. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57:267–272.
  • Kief, D. R., and J. R. Warner. 1981. Coordinate control of synthesis of ribosomal ribonucleic acid and ribosomal proteins during nutritional shift-up in Saccharomyces cerevisiae. Mol. Cell. Biol. 1:1007–1015.
  • Kimura, J., and M. Kimura. 1987. The complete amino acid sequences of the 5S rRNA binding proteins L5 and L18 from the moderate thermophile Bacillus stearothermophilus ribosome. FEBS Lett. 210:85–90.
  • Kozak, M. 1987. An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15:8125–8148.
  • Kozak, M. 1990. Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes. Proc. Natl. Acad. Sci. USA 87:8301–8305.
  • Kraft, R., J. Tardiff, K. S. Krauter, and L. A. Leinwand. 1988. Using mini-prep plasmid DNA for sequencing double-stranded templates with Sequenase. BioTechniques 6:544–547.
  • Kristiansen, K. (Odense University). 1991. Personal communication..
  • Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685.
  • Larkin, J. C., J. R. Thompson, and J. L. Woolford, Jr. 1987. Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene. Mol. Cell. Biol. 7:1764–1775.
  • Leer, R. J., M. M. C. van Raamsdonk-Duin, W. H. Mager, and R. J. Pianta. 1984. The primary structure of the gene encoding yeast ribosomal protein L16. FEBS Lett. 175:371–376.
  • Li, H. V., J. Zagorski, and M. Fournier. 1990. Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae. Mol. Cell. Biol. 10:1145–1152.
  • Maicas, E., F. G. Pluthero, and J. D. Friesen. 1988. The accumulation of three yeast ribosomal proteins under conditions of excess mRNA is determined primarily by fast protein decay. Mol. Cell. Biol. 8:169–175.
  • Marvaldi, J., J. Pichon, and G. Marchis-Mouren. 1979. On the control of ribosomal protein biosynthesis in E. coli. IV. Studies on a temperature-sensitive mutant defective in the assembly of 50S subunits. Mol. Gen. Genet. 171:317–325.
  • Moritz, M., A. G. Paulovich, Y.-F. Tsay, and J. L. Woolford, Jr. 1990. Depletion of yeast ribosomal proteins L16 or rp59 disrupts ribosome assembly. J. Cell Biol. 111:2261–2274.
  • Nam, H. G., and H. M. Fried. 1986. Effects of progressive depletion of TCM1 or CYH2 mRNA on Saccharomyces cerevisiae ribosomal protein accumulation. Mol. Cell. Biol. 6:1535–1544.
  • Nashimoto, H., A. Miura, H. Saito, and H. Uchida. 1985. Suppressors of temperature-sensitive mutations in a ribosomal protein gene rpsL (S12), of Escherichia coli K12. Mol. Gen. Genet. 199:381–387.
  • Nashimoto, H., and M. Nomura. 1970. Structure and function of bacterial ribosomes. XI. Dependence of 50S ribosomal subunit assembly on simultaneous assembly of 30S subunits. Proc. Natl. Acad. Sci. USA 67:1440–1447.
  • Nazar, R. N., M. Yaguchi, G. E. Willick, C. F. Rollin, and C. Roy. 1979. The 5S RNA binding protein from yeast (Saccharomyces cerevisiae) ribosomes. Evolution of the eukaryotic 5S RNA binding protein. Eur. J. Biochem. 102:573–582.
  • Nomura, M., P. Traub, C. Guthrie, H. Nashimoto. 1969. The assembly of ribosomes. J. Cell. Physiol. 74:241–252.
  • Ohkubo, S., A. Muto, Y. Kawauchi, F. Yamao, and S. Osawa. 1987. The ribosomal protein gene cluster of Mycoplasma capricolum. Mol. Gen. Genet. 210:314–322.
  • Pearson, W. R., and D. J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444–2448.
  • Pichon, J., J. Marvaldi, and G. Marchis-Mouren. 1979. On the control of ribosomal protein biosynthesis in E. coli. III. Studies on a temperature-sensitive mutant defective in the assembly of 30S subunits. Mol. Gen. Genet. 171:307–316.
  • Pringle, J. R., and L. H. Hartwell. 1981. The Saccharomyces cerevisiae cell cycle, p. 97-142. In J. N. Strathem, E. W. Jones, and J. R. Broach (ed.), The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Rose, M. D., and G. R. Fink. 1987. KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast. Cell. 48:1047–1060.
  • Rotenberg, M. O., M. Moritz, and J. L. Woolford, Jr. 1988. Depletion of Saccharomyces cerevisiae ribosomal protein L16 causes a decrease in 60S ribosomal subunits and formation of half-mer polyribosomes. Genes Dev. 2:160–172.
  • Sachs, A. B., and R. W. Davis. 1989. The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation. Cell 58:857–867.
  • Sachs, A. B., and R. W. Davis. 1990. Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46. Science 247:1077–1079.
  • Schnier, J., and K. Nishi. 1988. Temperature-sensitive mutants with alterations in ribosomal protein L24 and isolation of intra-and extragenic suppressor mutants. Methods Enzymol. 164:706–709.
  • Sherman, F., G. R. Fink, and J. B. Hicks. 1986. Methods in yeast genetics, p. 166-167. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Sherman, F., and J. W. Stewart. 1982. Mutations altering initiation of translation of yeast iso-1-cytochrome c: contrasts between the eukaryotic and prokaryotic initiation process, p. 301-333. In J. N. Strathem, E. W. Jones, and J. R. Broach (ed.), The molecular biology of the yeast Saccharomyces: metabolism and gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Singh, A., and T. R. Manney. 1974. Genetic analysis of mutations affecting growth of Saccharomyces cerevisiae at low temperature. Genetics 77:651–659.
  • Singleton, C. K., S. S. Manning, and R. Ken. 1989. Primary structure and regulation of vegetative specific genes of Dictyos- telium discoideum. Nucleic Acids Res. 17:9679–9692.
  • Stern, S., T. Powers, L.-M. Changchien, and H. F. Noller. 1989. RNA-protein interactions in 30S ribosomal subunits: folding and function of 16S rRNA. Science 244:783–790.
  • Surguchov, A. P., E. S. Fominykch, V. N. Smirnov, M. D. Ter-Avanesyan, L. N. Mironova, and S. G. Inge-Vechtomov. 1981. Further characterization of recessive suppression in yeast. Isolation of the low-temperature sensitive mutant of Saccharomyces cerevisiae defective in the assembly of 60S ribosomal subunit. Biochim. Biophys. Acta 654:149–155.
  • Tai, P.-C., D. P. Kessler, and J. Ingraham. 1969. Cold-sensitive mutations in Salmonella typhimurium which affect ribosome synthesis. J. Bacteriol. 97:1298–1304.
  • Tollervey, D., H. Lehtonen, M. Carmo-Fonseca, and E. C. Hurt. 1991. The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J. 10:573–583.
  • Toniolo, D., H. K. Meiss, and C. Basilico. 1973. A temperature-sensitive mutation affecting 28S ribosomal RNA production in mammalian cells. Proc. Natl. Acad. Sci. USA 70:1273–1277.
  • Tsay, Y.-F., J. R. Thompson, M. O. Rotenberg, J. C. Larkin, and J. L. Woolford, Jr. 1988. Ribosomal protein synthesis is not regulated at the translational level in Saccharomyces cerevisiae: balanced accumulation of ribosomal proteins L16 and rp59 is mediated by turnover of excess protein. Genes Dev. 2:664–676.
  • Tsay, Y.-F., and J. Woolford. Unpublished data.
  • Udem, S. A., and J. R. Warner. 1972. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J. Mol. Biol. 65:227–242.
  • Underwood, M. R., and H. M. Fried. 1990. Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29. EMBO J. 9:91–99.
  • Ursic, D., and J. Davies. 1979. Cold-sensitive mutant of Saccharomyces cerevisiae defective in ribosome processing. Mol. Gen. Genet. 175:313–323.
  • Van der Zeijst, B. A. M., A. J. Kool, and H. P. J. Bloemers. 1972. Isolation of active ribosomal subunits from yeast. Eur. J. Biochem. 30:15–25.
  • Warner, J. R. 1989. The synthesis of ribosomes in Saccharomyces cerevisiae. Microbiol. Rev. 53:256–271.
  • Warner, J. R., G. Mitra, W. F. Schwindinger, M. Studeny, and H. M. Fried. 1985. Saccharomyces cerevisiae coordinates the accumulation of yeast ribosomal proteins by modulating mRNA splicing, translational initiation, and protein turnover. Mol. Cell. Biol. 5:1512–1521.
  • Warner, J. R., and S. A. Udem. 1972. Temperature sensitive mutations affecting ribosome synthesis in Saccharomyces cerevisiae. J. Mol. Biol. 65:243–257.
  • Wittekind, M., J. M. Kolb, J. Dodd, M. Yamagishi, S. Memet, J.-M. Buhler, and M. Nomura. 1990. Conditional expression of RPA190, the gene encoding the largest subunit of yeast RNA polymerase I: effects of decreased rRNA synthesis on ribosomal protein synthesis. Mol. Cell. Biol. 10:2049–2059.
  • Woolford, J. L., L. M. Hereford, and M. Rosbash. 1979. Isolation of cloned DNA sequences containing ribosomal protein genes from Saccharomyces cerevisiae. Cell 18:1247–1259.
  • Zitomer, R. S., D. A. Walthall, B. C. Rymond, and C. P. Hollenberg. 1984. Saccharomyces cerevisiae ribosomes recognize non-AUG initiation codons. Mol. Cell. Biol. 4:1191–1197.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.