2
Views
10
CrossRef citations to date
0
Altmetric
Gene Expression

Purification of Splicing Factor SF1, a Heat-Stable Protein That Functions in the Assembly of a Presplicing Complex

Pages 4545-4552 | Received 05 May 1992, Accepted 19 Jul 1992, Published online: 01 Apr 2023

REFERENCES

  • Ast, G., D. Goldblatt, D. Offen, J. Sperling, and R. Sperling. 1991. A novel splicing factor is an integral component of 200S large nuclear ribonucleoprotein (InRNP) particles. EMBO J. 10: 425–432.
  • Bindereif, A., and M. R. Green. 1987. An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly. EMBO J. 6: 2415–2424.
  • Blencowe, B. J., B. S. Sproat, U. Ryder, S. Barabino, and A. I. Lamond. 1989. Antisense probing of the human U4/U6 snRNP with biotinylated 2′-OMe RNA oligonucleotides. Cell 59: 531–539.
  • Bothwell, A. L. M., D. W. Ballard, W. M. Philbrick, G. Lindwall, S. E. Maher, M. M. Bridgett, S. F. Jamison, and M. A. Garcia-Blanco. 1991. Murine polypyrimidine tract binding protein. Purification, cloning, and mapping of the RNA binding domain. J. Biol. Chem. 266: 24657–24663.
  • Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
  • Brunel, F., P. M. Alzari, P. Ferrara, and M. M. Zakin. 1992. Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein. Nucleic Acids Res. 19: 5237–5245.
  • Cheng, S.-C., and J. Abelson. 1987. Spliceosome assembly in yeast. Genes Dev. 1: 1014–1027.
  • Choi, Y. D., P. J. Grabowski, P. A. Sharp, and G. Dreyfuss. 1986. Heterogeneous nuclear ribonucleoproteins: role in splicing. Science 231: 1534–1539.
  • Delannoy, P., and M. H. Caruthers. 1991. Detection and characterization of a factor which rescues spliceosome assembly from a heat-inactivated HeLa cell nuclear extract. Mol. Cell. Biol. 11: 3425–3431.
  • Frendewey, D., and W. Keller. 1985. Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences. Cell 42: 355–367.
  • Fu, X.-D., and T. Maniatis. 1990. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature (London) 343: 437–441.
  • Fu, X.-D., and T. Maniatis. 1992. The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3′ splice site. Proc. Natl. Acad. Sci. USA 89: 1725–1729.
  • Garcia-Blanco, M. A., S. Jamison, and P. A. Sharp. 1989. Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3: 1874–1886.
  • Ge, H., and J. L. Manley. 1990. A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell 62: 25–34.
  • Gerke, V., and J. A. Steitz. 1986. A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of pre-messenger RNA. Cell 47: 937–984.
  • Green, M. R. 1991. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing. Annu. Rev. Cell Biol. 7: 559–599.
  • Guthrie, C. 1991. Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein. Science 253: 157–163.
  • Harper, J. E., and J. L. Manley. 1991. A novel protein factor is required for use of distal alternative 5′ splice sites in vitro. Mol. Cell. Biol. 11: 5945–5953.
  • Jansen-Durr, P., M. Boshart, B. Lupp, A. Bosserhoff, R. W. Frank, and G. Schutz. 1992. The rat polypyrimidine tract binding protein (PTB) interacts with a single-stranded DNA motif in a liver-specific enhancer. Nucleic Acids Res. 20: 1243–1249.
  • Kim, S.-H., J. Smith, A. Claude, and R.-J. Lin. 1992. The purified yeast pre-mRNA splicing factor PRP2 is an RNA- dependent NTPase. EMBO J. 11: 2319–2326.
  • Konarska, M. M., and P. A. Sharp. 1987. Interactions between small nuclear ribonucleoprotein particles in the formation of spliceosomes. Cell 49: 763–774.
  • Krainer, A. R., G. C. Conway, and D. Kozak. 1990. The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites. Cell 62: 35–42.
  • Krainer, A. R., G. C. Conway, and D. Kozak. 1990. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells. Genes Dev. 4: 1158–1171.
  • Krainer, A. R., and T. Maniatis. 1985. Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro. Cell 42: 725–736.
  • Krämer, A. 1988. Pre-splicing complex formation requires two proteins and U2 snRNP. Genes Dev. 2: 1155–1167.
  • Krämer, A., M. Frick, and W. Keller. 1987. Separation of multiple components of HeLa cell nuclear extracts required for pre-messenger RNA splicing. J. Biol. Chem. 262: 17630–17640.
  • Krämer, A., and U. Utans. 1991. Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs. EMBO J. 10: 1503–1509.
  • Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680–685.
  • Mayeda, A., and A. R. Krainer. 1992. Regulation of alternative pre-mRNA splicing by hnRNP Al and splicing factor SF2. Cell 68: 365–375.
  • Michaud, S., and R. Reed. 1991. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes Dev. 5: 2534–2546.
  • Patton, J. G., S. A. Meyer, P. Tempst, and B. Nadal-Ginard. 1991. Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing. Genes Dev. 5: 1237–1251.
  • Pikielny, C. W., B. C. Rymond, and M. Rosbash. 1986. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes. Nature (London) 324: 341–345.
  • Ruby, S. W., and J. Abelson. 1988. An early and hierarchic role of the U1 snRNP in spliceosome assembly. Science 242: 1028–1035.
  • Ruskin, B., P. D. Zamore, and M. R. Green. 1988. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly. Cell 52: 207–219.
  • Schwer, B., and C. Guthrie. 1991. PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature (London) 349: 494–499.
  • Séraphin, B., and M. Rosbash. 1989. Identification of functional U1 snRNP-pre-mRNA complexes committed to spliceosome assembly and splicing. Cell 59: 349–358.
  • Siegel, L. M., and K. J. Monty. 1966. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim. Biophys. Acta 112: 346–362.
  • Sierakowska, H., W. Szer, P. J. Furdon, and R. Kole. 1986. Antibodies to hnRNP core proteins inhibit in vitro splicing of human beta-globin pre-mRNA. Nucleic Acids Res. 14: 5241–5254.
  • Swanson, M. S., and G. Dreyfuss. 1988. RNA binding specificity of hnRNP proteins: a subset bind to the 3′ end of introns. EMBO J. 7: 3519–3529.
  • Tazi, J., C. Alibert, J. Temsamani, I. Reveillaud, G. Cathala, C. Brunel, and P. Jeanteur. 1986. A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein. Cell 47: 755–766.
  • Utans, U., S.-E. Behrens, R. Luhnnann, R. Kole, and A. Krämer. 1992. A splicing factor which is inactivated during in vivo heat shock is functionally equivalent to the [U4/U6.U5] triple-snRNP proteins. Genes Dev. 6: 631–641.
  • Wang, J., K. Nishiyama, K. Araki, D. Kitamura, and T. Watanabe. 1987. Purification of an octamer sequence (ATGCAAAT)- binding protein from human B cells. Nucleic Acids Res. 15: 10105–10116.
  • Zamore, P. D., and M. R. Green. 1989. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. Proc. Natl. Acad. Sci. USA 86: 9243–9247.
  • Zamore, P. D., and M. R. Green. 1991. Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution. EMBO J. 10: 207–214.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.