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Gene Expression

The Octamer/μE4 Region of the Immunoglobulin Heavy-Chain Enhancer Mediates Gene Repression in Myeloma × T-Lymphoma Hybrids

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Pages 3530-3540 | Received 16 Nov 1992, Accepted 23 Mar 1993, Published online: 01 Apr 2023

REFERENCES

  • Bergman, Y., B. Strich, H. Sharir, R. Ber, and R. Laskov. 1990. Extinction of Ig genes expression in myeloma × fibroblast somatic cell hybrids is accompanied by repression of the Oct-2 gene encoding a B-cell specific transcription factor. EMBO J. 9:849–855.
  • Boshart, M., F. Weih, M. Nichols, and G. Schutz. 1991. The tissue-specific extinguisher locus Tsel encodes a regulatory subunit of cAMP-dependent protein kinase. Cell 66:849–859.
  • Bulla, G. A., V. DeSimone, R. Cortese, and R. E. K. Fournier. 1992. Extinction of αl-antitrypsin gene expression in somatic cell hybrids: evidence for multiple controls. Genes Dev. 6:316–327.
  • Cereghini, S., M. Yaniv, and R. Cortese. 1990. Hepatocyte dedifferentiation and extinction is accompanied by a block in the synthesis of mRNA coding for the transcription factor HNF1/ LFB1. EMBO J. 9:2257–2263.
  • Chu, G., H. Hayakawa, and P. Berg. 1987. Electroporation for efficient transformation of mammalian cells with DNA. Nucleic Acids Res. 15:1311–1327.
  • Church, G., A. Ephrussi, W. Gilbert, and S. Tonegawa. 1985. Cell type specific contacts to immunoglobulin enhancers in nuclei. Nature (London) 313:798–801.
  • Davidson, I., C. Fromental, P. Augereau, A. Wildeman, M. Zenke, and P. Chambon. 1986. Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts. Nature (London) 323:544–548.
  • Eckhardt, L., S. Tilley, R. Lang, K. Marcu, and B. Birshtein. 1982. DNA rearrangements in MPC-11 immunoglobulin heavy chain class-switch variants. Proc. Natl. Acad. Sci. USA 79:3006–3010.
  • Ephrussi, A., G. Church, S. Tonegawa, and W. Gilbert. 1985. B lineage-specific interactions of an immunoglobulin enhancer with cellular factors in vivo. Science 227:134–140.
  • Fromm, M., and P. Berg. 1982. Deletion mapping of DNA regions required for SV40 early region promoter function in vivo. J. Mol. Appl. Genet. 1:457–481.
  • Gerster, T., P. Matthias, M. Thali, J. Jiricny, and W. Schaffner. 1987. Cell type-specificity elements of the immunoglobulin heavy chain gene enhancer. EMBO J. 6:1323–1330.
  • Gerster, T., and R. Roeder. 1988. A herpesvirus trans-activating protein interacts with transcription factor OTF-1 and other cellular proteins. Proc. Natl. Acad. Sci. USA 85:6347–6351.
  • Gourdeau, H., and R. E. K. Fournier. 1990. Genetic analysis of mammalian cell differentiation. Annu. Rev. Cell Biol. 6:69–94.
  • Greenberg, A., R. Ber, Z. Kra-Oz, and R. Laskov. 1987. Extinction of expression of immunoglobulin genes in myeloma × fibroblast somatic cell hybrids. Mol. Cell. Biol. 7:936–939.
  • Gruda, M., and J. Alwine. 1991. Simian virus 40 (SV40) T-antigen transcriptional activation mediated through the Oct/SPH region of the SV40 late promoter. J. Virol. 65:3553–3558.
  • Hanke, J., N. Landolfi, P. Tucker, and J. Capra. 1988. Identification of murine nuclear proteins that bind to the conserved octamer sequence of the immunoglobulin promoter region. Proc. Natl. Acad. Sci. USA 85:3560–3564.
  • Hyman, R., and V. Stallings. 1974. Complementation patterns of Thy-1 variants and evidence that antigen loss variants “pre-exist” in the parental population. J. Natl. Cancer Inst. 52:429–436.
  • Ip, Y. T., D. Poon, D. Stone, D. K. Granner, and R. Chalkley. 1990. Interaction of liver-specific factor with an enhancer 4.8 kilobases upstream of the phosphoenolpyruvate carboxykinase gene. Mol. Cell. Biol. 10:3770–3781.
  • Jones, K. W., M. H. Shapero, M. Chevrette, and R. E. K. Fournier. 1991. Subtractive hybridization cloning of a tissue-specific extinguisher: Tsel encodes a regulatory subunit of protein kinase A. Cell 66:861–872.
  • Junker, S., V. Nielsen, P. Matthias, and D. Picard. 1988. Both immunoglobulin promoter and enhancer sequences are targets for suppression in myeloma-fibroblast hybrid cells. EMBO J. 7:3093–3098.
  • Junker, S., V. Nielsen, P. Matthias, and D. Picard. 1989. Myeloma κ gene transcription is blocked upon fusion with fibroblasts. Exp. Cell Res. 180:63–71.
  • Junker, S., S. Pedersen, E. Schreiber, and P. Matthias. 1990. Extinction of an immunoglobulin κ promoter in cell hybrids is mediated by the octamer motif and correlates with suppression of Oct-2 expression. Cell 61:467–474.
  • Kadesch, T. (U. of Pennsylvania School of Medicine). 1992. Personal communication.
  • Kemler, I., and W. Schaffner. 1990. Octamer transcription factors and the cell type-specificity of immunoglobulin gene expression. FASEB J. 4:1444–1449.
  • Kiledjian, M., L.-K. Su, and T. Kadesch. 1988. Identification and characterization of two functional domains within the murine heavy-chain enhancer. Mol. Cell. Biol. 8:145–152.
  • Killary, A. M., and R. Fournier. 1984. A genetic analysis of extinction: trans-dominant loci regulate expression of liver-specific traits in hepatoma hybrid cells. Cell 38:523–534.
  • Kristie, T., J. LeBoweitz, and P. Sharp. 1989. The octamer-binding proteins form multi-protein-DNA complexes with the HSV αTIF regulatory protein. EMBO J. 8:4229–4238.
  • Li, R., J. D. Knight, S. P. Jackson, R. Tjian, and M. R. Botchan. 1991. Direct interaction between Sp1 and the BPV enhancer E2 protein mediates synergistic activation of transcription. Cell 65:493–505.
  • Lu, B., C. R. Buck, C. F. Dreyfus, and I. B. Black. 1989. Expression of NGF and NGF receptor mRNAs in the developing brain: evidence for local delivery and action of NGF. Exp. Neurol. 104:191–199.
  • Luo, Y., H. Fujii, T. Gerster, and R. Roeder. 1992. A novel B cell-derived coactivator potentiates the activation of immuno-globulin promoters by octamer-binding transcription factors. Cell 71:231–241.
  • Marcu, K., J. Banerji, N. Penncavage, R. Lang, and N. Arnheim. 1980.5′ flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains. Cell 22:187–196.
  • McCormick, A., D. Wu, J.-L. Castrillo, S. Dana, J. Strobl, E. Thompson, and M. Karin. 1988. Extinction of growth hormone expression in somatic cell hybrids involves repression of the specific trans-activator GHF-1. Cell 55:379–389.
  • Melton, D., P. Krieg, M. Rebagliati, T. Maniatis, K. Zinn, and M. Green. 1984. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12:7035–7056.
  • Muller-Immergluck, M. M., W. Schaffner, and P. Matthias. 1990. Transcription factor Oct-2A contains functionally redun-dant activating domains and works selectively from a promoter but not from a remote enhancer position in non-lymphoid (HeLa) cells. EMBO J. 9:1625–1634.
  • Mulligan, R. C., and P. Berg. 1980. Expression of a bacterial gene in mammalian cells. Science 209:1422–1427.
  • Mulligan, R., and P. Berg. 1981. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 78:2072–2076.
  • Oi, V. T., S. Morrison, L. Herzenberg, and P. Berg. 1983. Immunoglobulin gene expression in transformed lymphoid cells. Proc. Natl. Acad. Sci. USA 80:825–829.
  • Pierani, A., A. Heguy, H. Fujii, and R. G. Roeder. 1990. Activation of octamer containing promoters by either octamer binding transcription factor 1 (OTF-1) or OTF-2 and requirement of an additional B-cell-specific component for optimal transcription of immunoglobulin promoters. Mol. Cell. Biol. 10:6204–6215.
  • Potter, H., L. Weir, and P. Leder. 1984. Enhancer-dependent expression of human κ immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. USA 81:7161–7165.
  • Ruezinsky, D., H. Beckmann, and T. Kadesch. 1991. Modulation of the IgH enhancer’s cell type specificity through a genetic switch. Genes Dev. 5:29–37.
  • Scholer, H. R., T. Ciesiolka, and P. Gruss. 1991. A nexus between Oct-4 and E1A: implications for gene regulation in embryonic stem cells. Cell 66:291–304.
  • Shen, L. Unpublished data.
  • Su, L.-K., and T. Kadesch. 1990. The immunoglobulin heavychain enhancer functions as the promoter for Iμ sterile tran-scription. Mol. Cell. Biol. 10:2619–2624.
  • Tanaka, M., U. Grossniklaus, W. Herr, and N. Hernandez. 1988. Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements. Genes Dev. 2:1764–1778.
  • Tanaka, M., and W. Herr. 1990. Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation do-mains induce Oct-2 phosphorylation. Cell 60:375–386.
  • Thayer, M., and R. Fournier. 1989. Hormonal regulation of TSEl-repressed genes: evidence for multiple genetic controls in extinction. Mol. Cell. Biol. 9:2837–2846.
  • Tripputi, P., S. Guerin, and D. Moore. 1988. Two mechanisms for the extinction of gene expression in hybrid cells. Science 241:1205–1207.
  • Weintraub, H., and M. Groudine. 1976. Chromosome subunits in active genes have an altered conformation. Science 193:848–856.
  • Weiss, M. 1992. Extinction by indirect means. Nature (London) 355:22–23.
  • Wu, C. 1980. The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature (London) 286:854–860.
  • Yu, H., B. Porton, L. Shen, and L. Eckhardt. 1989. Role of the octamer motif in hybrid cell extinction of immunoglobulin gene expression; extinction is dominant in a two enhancer system. Cell 58:441–448.
  • Zaller, D., H. Yu, and L. Eckhardt. 1988. Genes activated in the presence of an immunoglobulin enhancer or promoter are negatively regulated by a T-lymphoma cell line. Mol. Cell. Biol. 8:1932–1939.

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