Eucaryotic Transcription Complexes Are Specifically Associated in Large Sedimentable Structures: Rapid Isolation of Polymerase I, II, and III Transcription Factors

LITERATURE CITED

• Ackerman, S., Bunick P., Zandomeni R., and Weinmann R.. 1983. RNA polymerase II ternary transcription complexes generated in vitro. Nucleic Acids Res. 11:6041–6064.
   PubMedGoogle Scholar
• Birkenmeier, E., Brown D., and Jordan E.. 1978. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes. Cell 15:1077–1086.
   PubMed Web of Science ®Google Scholar
• Bogenhagen, D., Wormington M., and Brown D.. 1982. Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state. Cell 28:413–421.
   PubMed Web of Science ®Google Scholar
• Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254.
   PubMed Web of Science ®Google Scholar
• Brown, D. 1984. The role of stable complexes that repress and activate eukaryotic genes. Cell 37:359–365.
   PubMed Web of Science ®Google Scholar
• Cizewski, V., and Sollner-Webb B.. 1983. A stable transcription complex directs mouse ribosomal RNA synthesis by RNA polymerase I. Nucleic Acids Res. 11:7043–7056.
   PubMedGoogle Scholar
• Davidson, B., Egly J., Mulvihill E., and Chambon P.. 1983. Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences. Nature (London) 301:680–686.
   PubMedGoogle Scholar
• Davis, A. H., Reudelhuber T., and Garrard W.. 1983. Variegated chromatin structures of mouse ribosomal RNA genes. J. Mol. Biol. 167:133–155.
   PubMedGoogle Scholar
• Gottesfeld, J., and Bloomer L.. 1982. Assembly of transcriptionally active 5S RNA gene chromatin in vitro. Cell 28:781–791.
   PubMed Web of Science ®Google Scholar
• Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685.
   PubMed Web of Science ®Google Scholar
• Laser, A., Martin P., and Roeder R.. 1983. Transcription of class III genes: formation of preinitiation complexes. Science 222:740–748.
   PubMedGoogle Scholar
• Manley, J. L., Fire A., Amparo C., Sharp P., and Gefter M.. 1980. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc. Natl. Acad. Sci. U.S.A. 77:3855–3859.
   PubMed Web of Science ®Google Scholar
• Miesfeld, R., and Arnheim N.. 1982. Identification of the in vivo and in vitro origin of transcription in human rDNA. Nucleic Acids Res. 13:3933–3949.
   Google Scholar
• Miesfeld, R., and Arnheim N.. 1984. Species-specific rDNA transcription is due to promoter-specific binding factors. Mol. Cell. Biol. 4:221–227.
   PubMed Web of Science ®Google Scholar
• Miller, K., and Sollner-Webb B.. 1981. Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites. Cell 27:165–174.
   PubMed Web of Science ®Google Scholar
• Mishima, Y., Kominami R., and Muramatsu M.. 1982. Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor. Nucleic Acids Res. 10:6659–6670.
   PubMed Web of Science ®Google Scholar
• Morrissey, J. 1981. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117:307–312.
   PubMed Web of Science ®Google Scholar
• Par doll, D. M., and Volgelstein B.. 1980. Sequence analysis of nuclear matrix associated DNA from rat liver. Exp. Cell Res. 128:466–470.
   PubMedGoogle Scholar
• Robinson, S., Nelkin B., and Vogelstein B.. 1982. The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells. Cell 28:99–106.
   PubMed Web of Science ®Google Scholar
• Rose, S., and Garrard W.. 1984. Differentiation-dependent chromatin alterations precede and accompany transcription of immunoglobulin light chain genes. J. Biol. Chem. 259:8534–8544.
   PubMed Web of Science ®Google Scholar
• Sakonju, S., and Brown D.. 1982. Contact points between a positive transcription factor and the Xenopus 5S RNA gene. Cell 31:395–405.
   PubMed Web of Science ®Google Scholar
• Schaack, J., Sharp S., Dingermann T., and Soli D.. 1983. Transcription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes. J. Biol. Chem. 258:2447–2453.
   PubMed Web of Science ®Google Scholar
• Schwartz, L., Sklar V., Jaehning J., Weinmann R., and Roeder R.. 1974. Isolation and partial characterization of the multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in the mouse myeloma, MOPC 315. J. Biol. Chem. 249:5889–5897.
   PubMed Web of Science ®Google Scholar
• Tolunay, H., Yang L., Anderson F., and Safer B.. 1984. Isolation of an active transcription initiation complex from a HeLa cell-free extract. Proc. Natl. Acad. Sci. U.S.A. 81:5916–5920.
   PubMedGoogle Scholar
• Wandelt, C, and Grummt I.. 1983. Formation of stable preinitiation complexes is a prerequisite for ribosomal DNA transcription in vitro. Nucleic Acids Res. 11:3795–3809.
   PubMedGoogle Scholar
• Wilkinson, J. K., Miller K., and Sollner-Webb B.. 1983. Di-nucleotide primers facilitate convenient identification of the mouse ribosomal initiation site. J. Biol. Chem. 258: 13919–13928.
   PubMedGoogle Scholar
• Wingender, X., Shi P., Houpert A., and Seifart K.. 1984. Isolation of a transcription complex for ribosomal 5S RNA. Eur. Mol. Biol. Organ. 3:1761–1768.
   PubMedGoogle Scholar