2
Views
4
CrossRef citations to date
0
Altmetric
Research Article

Construction of a cDNA to the Hamster CAD Gene and Its Application Toward Defining the Domain for Aspartate Transcarbamylase

, , , &
Pages 1735-1742 | Received 08 Jan 1985, Accepted 10 Apr 1985, Published online: 31 Mar 2023

LITERATURE CITED

  • Alwine, J. C, Kemp D. J., and Stark G. R.. 1977. Methods for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc. Natl. Acad. Sci. U.S.A. 74:5350–5354.
  • Anderson, S., Gait M. J., Mayol L., and Young I. G.. 1980. A short primer for sequencing DNA cloned in the single-stranded phage vector M13 mp2. Nucleic Acids Res. 8:1731–1743.
  • Aviv, H., and Leder P.. 1972. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Natl. Acad. Sci. U.S.A. 69:1408–1412.
  • Bailey, J. M., and Davidson N.. 1976. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal. Biochem. 70:75–85.
  • Biggin, M. D., Gibson T. J., and Hong G. F.. 1983. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Natl. Acad. Sci. U.S.A. 80:3963–3965.
  • Brothers, V. M., Tsubota S. I., Germeradd S. E., and Fristrom J. W.. 1978. Rudimentary locus of Drosophila melanogaster : partial purification of a carbamylphosphate synthase-aspartate transcarbamylase-dihydroorotase complex. Biochem. Genet. 16:321–332.
  • Chirgwin, J. M., Pryzybyla A. E., MacDonald R. J., and Rutter W. J.. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299.
  • Coleman, P. F., Suttle D. P., and Stark G. R.. 1977. Purification from hamster cells of the multifunctional protein that initiates de novo synthesis of pyrimidine nucleotides. J. Biol. Chem. 252:6379–6385.
  • Davidson, J. N., and Niswander L. A.. 1983. Partial cDNA sequence to a hamster gene corrects defect in Escherichia coli pyrB mutant. Proc. Natl. Acad. Sci. U.S.A. 80:6879–6901.
  • Davidson, J. N., and Patterson D.. 1979. Alteration in structure of multifunctional protein from Chinese hamster ovary cells defective in pyrimidine biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 76:1731–1735.
  • Davidson, J. N., Rumsby P. C., and Tamaren J.. 1981. Organization of a multifunctional protein in pyrimidine biosynthesis. J. Biol. Chem. 256:5220–5225.
  • Davis, R. W., Botstein D., and Roth J. R.. 1980. Isolation of plasmid and bacterial DNA, p. 116–127. In Davis R. W. (ed.), Advanced bacterial genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Dretzen, G., Bellard M., Sassone-Corsi P., and Chambon P.. 1981. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal. Biochem. 112:295–298.
  • Efstratiadis, A., and Vilia-Komaroff L.. 1979. Cloning of double-stranded cDNA, p. 1–14. In Setlow J. K. and Hollander A. (ed.), Genetic engineering. Plenum Publishing Corp., New York.
  • Fausto-Sterling, A. 1977. Studies on the female sterile mutant rudimentary of Drosophila melanogaster. II. An analysis of aspartate transcarbamylase and dihydroorotase activities in wild-type and rudimentary strains. Biochem. Genet. 15: 803–815.
  • Grayson, D. R., and Evans D. R.. 1983. The isolation and characterization of the aspartate transcarbamylase domain of the multifunctional protein, CAD. J. Biol. Chem. 258:4123–4129.
  • Grunstein, M., and Hogness D. S.. 1975. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. U.S.A. 72:3961–3965.
  • Heidecker, G., Messing J., and Gronenborn B.. 1980. A versatile primer for DNA sequencing in the M13 mp2 cloning system. Gene 10:68–73.
  • Hoover, T. A., Roof W. D., Foltermann K. F., O'Donovan G. A., Bencini D. A., and Wild J. R.. 1983. Nucleotide sequence of the structural gene (pyrB) that encodes the catalytic polypeptide of aspartate transcarbamoylase of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 80:2462–2466.
  • Jones, M. E. 1980. Pyrimidine nucleotide biosynthesis in animals: genes, enzymes, and regulation of UMP biosynthesis. Annu. Rev. Biochem. 49:253–279.
  • Kacian, D. L., and Myers J. C.. 1976. Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities. Proc. Natl. Acad. Sci. U.S.A. 73:2191–2195.
  • Land, H., Grez M., Hauser H., Lindenmaier W., and Schutz G.. 1981. 5′-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Res. 9:2251–2266.
  • Lue, P. F., and Kaplan J. G.. 1970. Heat-induced disaggregation of a multifunctional enzyme complex catalyzing the first steps in pyrimidine biosynthesis in baker's yeast. Can. J. Biochem. 48:155–159.
  • Makoff, A. J., and Radford A.. 1978. Genetics and biochemistry of carbamoyl phosphate biosynthesis and its utilization in the pyrimidine biosynthetic pathway. Microbiol. Rev. 42:307–328.
  • Mally, M. I., Grayson D. R., and Evans D. R.. 1981. Controlled proteolysis of the multifunctional protein that initiates pyrimidine biosynthesis in mammalian cells: evidence for discreet structural domains. Proc. Natl. Acad. Sci. U.S.A. 78:6647–6651.
  • Maniatis, T., Fritsch E. F., and Sam brook J.. 1982. Molecular cloning: a laboratory manual, p. 156, 254-255. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Maxam, A. M., and Gilbert W.. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 65:497–559.
  • McDonell, M. W., Simon M. N., and Studier F. W.. 1977. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral alkaline gels. J. Mol. Biol. 110:119–146.
  • Meagher, R. B., Tait R. C., Betlach M., and Boyer H. W.. 1977. Protein expression in E. coli minicells by recombinant plasmids. Cell 10:521–536.
  • Miller, J. H. 1972. Experiments in molecular genetics, p. 431–433. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Myers, J. C, and Spiegelman S.. 1978. Sodium pyrophosphate inhibition of RNA:DNA hybrid degradation by reverse transcriptase. Proc. Natl. Acad. Sci. U.S.A. 75:5329–5333.
  • Okayama, H., and Berg P.. 1982. High-efficiency cloning of full-length cDNA. Mol. Cell. Biol. 2:161–170.
  • Okayama, H., and Berg P.. 1983. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3:280–289.
  • Padgett, R. A., Wahl G. M., Coleman P. F., and Stark G. R.. 1979. N-(Phosphonacetyl)-l-aspartate-resistant hamster cells overaccumulate a single mRNA coding for the multifunctional protein that catalyzes the first steps of UMP synthesis. J. Biol. Chem. 254:974–980.
  • Padgett, R. A., Wahl G. M., and Stark G. R.. 1982. Structure of the gene for CAD, the multifunctional protein that initiates UMP synthesis in Syrian hamster cells. Mol. Cell. Biol. 2:293–301.
  • Rigby, P. W. J., Dieckmann M., Rhodes C., and Berg P.. 1977. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113:237–251.
  • Robey, E. A., and Schachman H. K.. 1984. Site-specific mutagenesis of aspartate transcarbamylase: replacement of tyrosine 165 in the catalytic chain by serine reduces enzymatic activity. J. Biol. Chem. 259:11180–11183.
  • Rothenberg, E., and Baltimore D.. 1977. Increased length of DNA made by virions of murine leukemia virus at limiting magnesium ion concentration. J. Virol. 21:168–178.
  • Rumsby, P. C, Campbell P. C., Niswander L. A., and Davidson J. N.. 1984. Organization of a multifunctional protein in pyrimidine biosynthesis. Biochem. J. 217:435–440.
  • Sanger, F., Coulson A. R., Barrell B. G., Smith A. J. H., and Roe B. A.. 1980. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J. Mol. Biol. 143:161–178.
  • Sanger, F., Nicklen S., and Coulson A. R.. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74:5463–5467.
  • Segraves, W. A., Louis C., Tsubota S., Schedl P., Rawls J. M., and Jarry B. P.. 1984. The rudimentary locus of Drosophila melanogaster. J. Mol. Biol. 175:1–17.
  • Sutcliffe, J. G. 1978. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp. Quant. Biol. 43:77–90.
  • Thomas, P. S. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. U.S.A. 77:5201–5205.
  • Wahl, G. M., Padgett R. A., and Stark G. R.. 1979. Gene amplification causes overproduction of the first three enzymes of UMP synthesis in Af-(phosphonacetyl)-l-aspartate-resistant hamster cells. J. Biol. Chem. 254:8679–8689.
  • Williams, L. G., Bernhardt S., and Davis R. H.. 1970. Copurification of pyrimidine-specific carbamyl phosphate synthetase and aspartate transcarbamylase of Neurospora crassa. Biochemistry 9:4329–4335.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.