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Research Article

In Vitro Expression of Human p53 cDNA Clones and Characterization of the Cloned Human p53 Gene

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Pages 1887-1893 | Received 19 Feb 1985, Accepted 30 Apr 1985, Published online: 31 Mar 2023

LITERATURE CITED

  • Benchimol, S., Pirn D., and Crawford L.. 1982. Radioimmunoassay of the cellular protein p53 in mouse and human cell lines. EMBO J. 1:1055–1062.
  • Benton, W. D., and Davis R. W.. 1977. Screening lambda gt recombinant clones by hybridization to a single plaque in situ. Science 196:180–182.
  • Coffman, R. L., and Weissman I. L.. 1981. A monoclonal antibody which recognizes B cells and B cell precursors in mice. J. Exp. Med. 153:269–279.
  • Crawford, L. V., Pirn D. C., Gurney E. G., Goodfellow P., and Taylor-Papadimitriou J.. 1981. Detection of a common feature in several human tumor cell lines—a 53,000 dalton protein. Proc. Natl. Acad. Sci. U.S.A. 78:41–45.
  • Czosnek, H. H., Bienz B., Givol D., Zakut-Houri R., Pravtcheva D. D., Ruddle F. H., and Oren M.. 1984. The gene and the pseudogene for mouse p53 cellular tumor antigen are located on different chromosomes. Mol. Cell. Biol. 4:1638–1640.
  • Davis, R., Simon M., and Davidson N.. 1971. Electron microscopic heteroduplex methods for mapping regions of base sequence homology. Methods Enzymol. 21:413–428.
  • DeLeo, A. B., Jay E., Appella G. C., Dubois C. G., Law L. W., and Old L. J.. 1979. Detection of a transformation related antigen in chemically induced sarcoma and other transformed cells of the mouse. Proc. Natl. Acad. Sci. U.S.A. 76:2420–2424.
  • Dippold, W. G., Jay G., Deleo A. B., Khoury G., and Old L. J.. 1981. p53 transformation related protein. Detection by monoclonal antibody in mouse and human cells. Proc. Natl. Acad. Sci. U.S.A. 78:1695–1699.
  • Eliyahu, D., Raz A., Grass P., Givol D., and Oren M.. 1984. Participation of p53 cellular tumor antigen in transformation of normal embryonic cells. Nature (London) 312:646–649.
  • Ellis, R. W., DeFeo D., Maryak J. M., Young H. A., Shih T. Y., Chang E. H., Lowy R. D., and Scolnick E. M.. 1980. Dual evolutionary origin of the rat genetic sequences of Harvey murine sarcoma virus. J. Virol. 36:408–420.
  • Green, M. R., Maniatis T., and Metton D. A.. 1983. Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte. Cell 32:681–694.
  • Harlow, E., Crawford L. V., Pim D. C., and Williamson N. H.. 1981. Monoclonal antibodies specific for simian virus 40 tumor antigens. J. Virol. 39:861–869.
  • Jenkins, J. R., Rudge K., and Currie G. A.. 1984. Cellular immortalization by a cDNA clone encoding the transformation associated phosphoprotein. Nature (London) 312:651–654.
  • Kessler, S. W. 1975. Rapid isolation of antigens from cells with a staphylococcal protein A antibody absorbents: parameters of the interaction of antibody-antigen complex with protein A. J. Immunol. 115:1617–1624.
  • Laemmli, U. K. 1971. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685.
  • Lane, D. P., and Crawford L. V.. 1979. T antigen is bound to a host protein in SV40 transformed cells. Nature (London) 278:261–263.
  • Maniatis, T., Fritsch E. F., and Sambrook J.. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Matlashewski, G., Lamb P., Pim D., Peacock J., Crawford L., and Benchimol S.. 1984. Isolation and characterization of a human p53 cDNA clone: expression of human p53 gene. EMBO J. 3:3257–3262.
  • Mercer, W. E., Nelson D., Deleo A. B., Old L. J., and Baserga R.. 1982. Microinjection of monoclonal antibodies to protein p53 inhibits serum induced DNA synthesis in 3T3 cells. Proc. Natl. Acad. Sci. U.S.A. 79:6309–6312.
  • Milner, J., and Milner S.. 1981. SV40-53K antigen: a possible role of 53K in normal cells. Virology 112:785–788.
  • Oren, M., Bienz B., Givoi D., Rehavi G., and Zakut R.. 1983. Analysis of recombinant DNA clones specific for the murine p53 cellular tumor antigen. EMBO J. 2:1633–1639.
  • Parada, L. F., Land H., Weinberg R. A., Wolf D., and Rotter V.. 1984. Cooperation between gene coding p53 tumor antigen and ras in cellular transformation. Nature (London) 312:649–651.
  • Pelham, H., and Jackson R.. 1976. An efficient mRNA-depend-ent translation system from reticulocyte lysates. Eur. J. Biochem. 67:247–256.
  • Reich, N. C, and Levine A. J.. 1984. Growth regulation of cellular antigen p53 in nontransformed cells. Nature (London) 308:199–201.
  • Rigby, P. W., Dieckmann M., Rhodes C., and Berg P.. 1977. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113:237–251.
  • Rotter, V. 1983. p53, a transformation related cellular encoded protein, can be used as a biochemical marker for the detection of primary mouse tumor cells. Proc. Natl. Acad. Sci. U.S.A. 80:2613–2617.
  • Rotter, V., Friedman H., Katz A., Zerivitz K., and Wolf D.. 1983. Variation in antigenic determinants of p53 transformation-related protein obtained from various species. J. Immunol. 181:329–333.
  • Rotter, V., Witte O. N., Coffman R., and Baltimore D.. 1980. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. J. Virol. 36:547–555.
  • Rotter, V., Wolf D., Pravtcheva D., and Ruddle F. H.. 1984. Chromosomal assignment of the murine gene encoding the transformation-related protein p53. Mol. Cell. Biol. 4:383–385.
  • Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.
  • Todaro, G. J., Green H., and Swift M. R.. 1966. Susceptibility of human diploid fibroblasts strain to transformation by SV80 virus. Science 153:1252–1255.
  • Wolf, D., Admon S., Oren M., and Rotter V.. 1984. Abelson murine leukemia virus-transformed cells that lack p53 protein synthesis express aberrant p53 mRNA species. Mol. Cell. Biol. 4:552–558.
  • Wolf, D., Harris N., and Rotter V.. 1984. Reconstitution of p53 expression in a nonproducer Ab-MuLV transformed cell line by transfection of a functional p53 gene. Cell 38:119–126.
  • Wolf, D., and Rotter V.. 1984. Inactivation of p53 gene expression by an insertion of Moloney murine leukemia virus-like DNA sequences. Mol. Cell. Biol. 4:1402–1410.
  • Wolf, D., and Rotter V.. 1985. Major deletions in the gene encoding the p53 antigen cause lack of p53 expression in HL-60 cells. Proc. Natl. Acad. Sci. U.S.A. 82:790–794.
  • Zakut-Houri, R., Oren M., Bienz B., Lavie V., Hazum S., and Givol D.. 1983. A single gene and a pseudogene for the cellular tumor antigen p53. Nature (London) 306:594–597.

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