5′ Nucleotide Sequences Influence Serum-Modulated Expression of a Human Dihydrofolate Reductase Minigene

LITERATURE CITED

• Alwine, J. C., D. J. Kemp, and G. R. Stark. 1977. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc. Natl. Acad. Sci. USA 74:5350–5354.
   PubMed Web of Science ®Google Scholar
• Anagnou, N. P., S. J. O'Brien, T. Shimada, W. G. Nash, M.-J. Chen, and A. W. Nienhuis. 1984. Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism. Proc. Natl. Acad. Sci. USA 81:5170–5174.
   PubMed Web of Science ®Google Scholar
• Barsoum, J., and A. Varshavsky. 1985. Preferential localization of variant nuçleosomes near the 5′-end of the mouse dihydrofolate reductase gene. J. Biol. Chem. 260:7688–7697.
   PubMed Web of Science ®Google Scholar
• Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254.
   PubMed Web of Science ®Google Scholar
• Carothers, A. M., G. Urlaub, N. Ellis, and L. A. Chasin. 1983. Structure of the dihydrofolate reductase gene in Chinese hamster ovary cells. Nucleic Acids Res. 11:1997–2012.
   PubMedGoogle Scholar
• Chabner, B. A. 1982. Methotrexate, p. 229–255. In B. A. Chabner (ed.), Pharmacologic principles of cancer treatment. The W. B. Saunders Co., Philadelphia.
   Google Scholar
• Chen, M.-J., T. Shimada, A. D. Moulton, A. Cline, R. K. Humphries, J. Maizel, and A. W. Nienhuis. 1984. The functional human dihydrofolate reductase gene. J. Biol. Chem. 259:3933–3943.
   PubMedGoogle Scholar
• Chen, M.-J., T. Shimada, A. D. Moulton, M. Harrison, and A. W. Nienhuis. 1982. Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. USA 79:7435–7439.
   PubMed Web of Science ®Google Scholar
• Collins, M. L., J.-S. R. Wu, C. L. Santiago, S. L. Hendrickson, and L. F. Johnson. 1983. Delayed processing of dihydrofolate reductase heterogeneous nuclear RNA in amino acid-starved mouse fibroblasts. Mol. Cell. Biol. 3:1792–1802.
   PubMedGoogle Scholar
• Cowan, K. H., M. E. Goldsmith, R. M. Levine, S. C. Aitken, E. Douglass, N. Clendeninn, A. W. Nienhuis, and M. E. Lippman. 1982. Dihydrofolate reductase gene amplification and possible rearrangement in estrogen-responsive methotrexate-resistant human breast cancer cells. J. Biol. Chem. 257:15079–15086.
   PubMedGoogle Scholar
• Crouse, G. F., E. J. Leys, R. N. McEwan, E. G. Frayne, and R. E. Kellems. 1985. Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene. Mol. Cell. Biol. 5:1847–1858.
   PubMedGoogle Scholar
• Crouse, G. F., R. N. McEwan, and M. L. Pearson. 1983. Expression and amplification of engineered mouse dihydrofolate reductase minigenes. Mol. Cell. Biol. 3:257–266.
   PubMedGoogle Scholar
• Crouse, G. F., C. C. Simonsen, R. N. McEwan, and R. T. Schimke. 1982. Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells. J. Biol. Chem. 257:7887–7897.
   PubMedGoogle Scholar
• Dolnick, B. J., and J. J. Pink. 1983. 5–Fluorouracil modulation of dihydrofolate reductase RNA levels in methotrexate-resistant KB cells. J. Biol. Chem. 258:13299–13306.
   PubMedGoogle Scholar
• Domin, B. A., S. P. Grill, K. F. Bastow, and Y.-C. Cheng. 1982. Effect of methotrexate on dihydrofolate reductase activity in methotrexate-resistant human KB cells. Mol. Pharmacol. 21:478–482.
   PubMedGoogle Scholar
• Dynan, W. S., and R. Tjian. 1985. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. Nature (London) 316:774–778.
   PubMed Web of Science ®Google Scholar
• Farnham, P. J., J. M. Abrams, and R. T. Schimke. 1985. Opposite-strand RNAs from the 5′ flanking region of the mouse dihydrofolate reductase gene. Proc. Natl. Acad. Sci. USA 82:3978–3982.
   PubMed Web of Science ®Google Scholar
• Farnham, P. J., and R. T. Schimke. 1985. Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle. J. Biol. Chem. 260:7675–7680.
   PubMedGoogle Scholar
• Frost, E., and J. Williams. 1978. Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue. Virology 91:39–50.
   PubMed Web of Science ®Google Scholar
• Gasser, C. S., C. C. Simonsen, J. W. Schilling, and R. T. Schimke. 1982. Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells. Proc. Natl. Acad. Sci. USA 79:6522–6526.
   PubMed Web of Science ®Google Scholar
• Goldsmith, M. E., R. K. Humphries, T. Ley, A. Cline, J. A. Kantor, and A. W. Nienhuis. 1983. “Silent” nucleotide substitution in a β+-thalassemia globin gene activates splice site in coding sequence RNA. Proc. Natl. Acad. Sci. USA 80:2318–2322.
   PubMed Web of Science ®Google Scholar
• Graham, F. L., and A. J. van der Eb. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467.
   PubMed Web of Science ®Google Scholar
• Gruss, P., and G. Khoury. 1980. Rescue of a splicing defective mutant by insertion of an heterologous intron. Nature (London) 286:634–637.
   PubMedGoogle Scholar
• Gudewicz, T. M., V. B. Morhenn, and R. E. Kellems. 1981. The effect of polyoma virus, serum factors, and dibutyryl cyclic AMP on dihydrofolate reductase synthesis, and the entry of quiescent cells into S phase. J. Cell. Physiol. 108:1–8.
   PubMedGoogle Scholar
• Hamer, D. H., and P. Leder. 1979. Splicing and the formation of stable RNA. Cell 18:1299–1302.
   PubMedGoogle Scholar
• Hendrickson, S. L., J.-S. R. Wu, and L. F. Johnson. 1980. Cell cycle regulation of dihydrofolate reductase mRNA metabolism in mouse fibroblasts. Proc. Natl. Acad. Sci. USA 77:5140–5144.
   PubMedGoogle Scholar
• Kaufman, R. J., and P. A. Sharp. 1982. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene. J. Mol. Biol. 159:601–621.
   PubMed Web of Science ®Google Scholar
• Kaufman, R. J., and P. A. Sharp. 1983. Growth-dependent expression of dihydrofolate reductase mRNA from modular cDNA genes. Mol. Cell. Biol. 3:1598–1608.
   PubMedGoogle Scholar
• Kellems, R. E., V. B. Morhenn, E. A. Pfendt, F. W. Alt, and R. T. Schimke. 1979. Polyoma virus and cyclic AMP-mediated control of dihydrofolate reductase mRNA abundance in methotrexate-resistant mouse fibroblasts. J. Biol. Chem. 254:309–318.
   PubMed Web of Science ®Google Scholar
• Lee, F., R. Mulligan, P. Berg, and G. Ringold. 1981. Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary toumor virus chimaeric plasmids. Nature (London) 294:228–232.
   PubMed Web of Science ®Google Scholar
• Lewis, J. A., D. T. Kurtz, and P. W. Melera. 1981. Molecular cloning of Chinese hamster dihydrofolate reductase-specific cDNA and the identification of multiple dihydrofolate reductase mRNAs in antifolate-resistant Chinese hamster lung fibroblasts. Nucleic Acids Res. 9:1311–1322.
   PubMedGoogle Scholar
• Leys, E. J., G. F. Crouse, and R. E. Kellems. 1984. Dihydrofolate reductase gene expression in cultured mouse cells is regulated by transcript stabilization in the nucleus. J. Cell Biol. 99:180–187.
   PubMedGoogle Scholar
• Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Mariani, B. D., D. L. Slate, and R. T. Schimke. 1981. S phase-specific synthesis of dihydrofolate reductase in Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78:4985–4989.
   PubMedGoogle Scholar
• Masters, J. N., and G. Attardi. 1983. The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase. Gene 21:59–63.
   PubMed Web of Science ®Google Scholar
• Masters, J. N., and G. Attardi. 1985. Discrete human dihydrofolate reductase gene transcripts present in polysomal RNA map with their 5′ ends several hundred nucleotides upstream of the main mRNA start site. Mol. Cell. Biol. 5:493–500.
   PubMed Web of Science ®Google Scholar
• Masters, J. N., J. K. Yang, A. Cellini, and G. Attardi. 1983. A human dihydrofolate reductase pseudogene and its relationship to the multiple forms of specific messenger RNA. J. Mol. Biol. 167:23–36.
   PubMed Web of Science ®Google Scholar
• Maurer, B. J., P. E. Barker, J. N. Masters, F. H. Ruddle, and G. Attardi. 1984. Human dihydrofolate reductase gene is located in chromosome 5 and is unlinked to the related pseudogenes. Proc. Natl. Acad. Sci. USA 81:1484–1488.
   PubMedGoogle Scholar
• McGrogan, M., C. C. Simonsen, D. T. Smouse, P. J. Farnham, and R. T. Schimke. 1985. Heterogeneity at the 5′ termini of mouse dihydrofolate reductase mRNAs: evidence for multiple promoter regions. J. Biol. Chem. 260:2307–2314.
   PubMedGoogle Scholar
• Milbrandt, J. D., J. C. Azizkhan, K. S. Greisen, and J. L. Hamlin. 1983. Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue. Mol. Cell. Biol. 3:1266–1273.
   PubMedGoogle Scholar
• Milbrandt, J. D., J. C. Azizkhan, and J. L. Hamlin. 1983. Amplification of a cloned Chinese hamster dihydrofolate reductase gene after transfer into a dihydrofolate reductase-deficient cell line. Mol. Cell. Biol. 3:1274–1282.
   PubMedGoogle Scholar
• Morandi, C., J. N. Masters, M. Mottes, and G. Attardi. 1982. Multiple forms of human dihydrofolate reductase messenger RNA: cloning and expression in Escherichia coli of their DNA coding sequence. J. Mol. Biol. 156:583–607.
   PubMedGoogle Scholar
• Myers, C. E., M. E. Lippman, H. M. Eliot, and B. A. Chabner. 1975. Competitive protein binding assay for methotrexate. Proc. Natl. Acad. Sci. USA 72:3683–3686.
   PubMed Web of Science ®Google Scholar
• Richter, A., K. K. Sanford, and V. J. Evans. 1972. Influence of oxygen and culture media on plating efficiency of some mammalian tissue cells. J. Natl. Cancer Inst. 49:1705–1712.
   PubMedGoogle Scholar
• Santiago, C., M. Collins, and L. F. Johnson. 1984. In vitro and in vivo analysis of the control of dihydrofolate reductase gene transcription in serum-stimulated mouse fibroblasts. J. Cell. Physiol. 118:79–86.
   PubMedGoogle Scholar
• Setzer, D. R., M. McGrogan, and R. T. Schimke. 1982. Nucleotide sequence surrounding multiple polyadenylation sites in the mouse dihydrofolate reductase gene. J. Biol. Chem. 257:5143–5147.
   PubMedGoogle Scholar
• Shimada, T., M.-J. Chen, and A. W. Nienhuis. 1984. A human dihydrofolate reductase intronless pseudogene with an Alu repetitive sequence: multiple DNA insertions at a single chromosomal site. Gene 31:1–8.
   PubMedGoogle Scholar
• Srimatkandada, S., W. D. Medina, A. R. Cashmore, W. Whyte, D. Engel, B. A. Moroson, C. T. Franco, S. K. Dube, and J. R. Bertino. 1983. Amplification and organization of dihydrofolate reductase genes in a human leukemic cell line, K–562, resistant to methotrexate. Biochemistry 22:5774–5781.
   PubMed Web of Science ®Google Scholar
• Urlaub, G., E. Kas, A. M. Carothers, and L. A. Chasin. 1983. Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells. Cell 33:405–412.
   PubMed Web of Science ®Google Scholar
• Wigier, M., M. Perucho, D. Kurtz, S. Dana, A. Pellicer, R. Axel, and S. Silverstein. 1980. Transformation of mammalian cells with an amplifiable dominant-acting gene. Proc. Natl. Acad. Sci. USA 77:3567–3570.
   PubMedGoogle Scholar
• Wu, J.-S. R., L. M. Wiedemann, and L. F. Johnson. 1982. Inhibition of dihydrofolate reductase gene expression following serum withdrawal or db-cAMP addition in methotrexate-resistant mouse fibroblasts. Exp. Cell Res. 141:159–169.
   PubMedGoogle Scholar
• Yang, J. K., J. N. Masters, and G. Attardi. 1984. Human dihydrofolate reductase gene organization: extensive conservation of the G + C-rich 5′ non-coding sequence and strong intron size divergence from homologous mammalian genes. J. Mol. Biol. 176:169–187.
   PubMedGoogle Scholar
• Yoder, S. S., and S. M. Berget. 1985. Posttranscriptional control of DHFR gene expression during adenovirus 2 infection. J. Virol. 54:72–77.
   PubMedGoogle Scholar