Use of a Hybrid Vaccinia Virus-T7 RNA Polymerase System for Expression of Target Genes

LITERATURE CITED

• Burnette, W. N. 1981. “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacryl amide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Bio- chem. 112:195–204.
   Google Scholar
• Chakrabarti, S., K. Brechling, and B. Moss. 1985. Vaccinia virus expression vector: coexpression of β-galactosidase provide visual screening of recombinant virus plaques. Mol. Cell. Biol. 5:3403–3409.
   PubMedGoogle Scholar
• Cochran, M. A., C. Puckett, and B. Moss. 1985. In vitro mutagenesis of the promoter region for a vaccinia virus gene: evidence for tandem early and late regulatory signals. J. Virol. 53:30–37.
   Google Scholar
• Dunn, J. J., and F. W. Studier. 1983. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J. Mol. Biol. 166:477–535.
   PubMed Web of Science ®Google Scholar
• Fuerst, T. R., E. G. Niles, F. W. Studier, and B. Moss. 1986. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase . Proc. Natl. Acad. Sci. USA 83:8122–8126.
   PubMed Web of Science ®Google Scholar
• Hsiung, N., R. Fitts, S. Wilson, A. Milne, and D. Hamer. 1984. Efficient production of hepatitis B surface antigen using a bovine papilloma virus-metallothionein vector. J. Mol. Appl. Genet. 2:497–506.
   PubMedGoogle Scholar
• Kozak, M. 1984. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 12:857–872.
   PubMed Web of Science ®Google Scholar
• Lehrach, D., D. Diamond, J. M. Wozney, and H. Boedtkes. 1977. RNA molecular weight determinations by gel electrophoresis under denaturing conditions: a critical re-examination. Bio- chemistry 16:4743–4751.
   Google Scholar
• Mackett, M., G. L. Smith, and B. Moss. 1984. General method for the production and selection of infectious vaccinia virus recombinants expressing foreign genes. J. Virol. 49:857–864.
   PubMed Web of Science ®Google Scholar
• Mackett, M., G. L. Smith, and B. Moss. 1985. The construction and characterization of vaccinia virus recombinants expressing foreign genes, p. 191–211. In D. Rickwood and B. D. Hames (ed.), DNA cloning, vol. 2. IRL Press, Washington, D.C.
   Google Scholar
• Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual, p. 90–93. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Moriarty, A. M., B. H. Hoyer, J. W. Shih, J. L. Gerin, and D. H. Hamer. 1981. Expression of the hepatitis B virus surface antigen in cell cultures by using a simian virus 40 vector. Proc. Natl. Acad. Sci. USA 78:2606–2610.
   PubMedGoogle Scholar
• Oda, K., and W. K. Joklik. 1967. Hybridization and sedimentation studies on “early” and “late” vaccinia messenger RNA. J. Mol. Biol. 27:395–419.
   PubMed Web of Science ®Google Scholar
• Peterson, D. L., N. Nath, and F. Gavilanes. 1982. Structure of hepatitis B surface antigen. J. Biol. Chem. 257:10414–10420.
   PubMedGoogle Scholar
• Ratner, L., W. Haseltine, R. Patarca, K. J. Livak, B. Starcich, S. F. Josephs, E. R. Doran, J. A. Rafalski, E. A. Whitehorn, K. Baumeister, L. Ivanoff, S. R. Petteway, Jr., M. L. Pearson, J. A. Lautenberger, T. S. Papas, J. Ghrayeb, N. T. Chang, R. C. Gallo, and F. Wong-Staal. 1985. Complete nucleotide sequence of the AIDS virus, HTLV-IIL Nature (London) 313:277–284.
   PubMed Web of Science ®Google Scholar
• Rhim, J. S., H. Y. Cho, and R. J. Huebner. 1975. Non-producer human cells induced by murine sarcoma virus. Int. J. Cancer 15:23–29.
   PubMed Web of Science ®Google Scholar
• Rice, A. P., and B. E. Roberts. 1983. Vaccinia virus induces cellular mRNA degradation. J. Virol. 47:529–539.
   PubMedGoogle Scholar
• Sebring, E. D., and N. P. Salzman. 1967. Metabolic properties of early and late vaccinia messenger ribonucleic acid. J. Virol. 1:550–575.
   PubMedGoogle Scholar
• Shih, J. W., P. J. Cote, G. M. Dapolito, and J. L. Gerin. 1980. Production of monoclonal antibodies against hepatitis B surface antigen (HBsAg) by somatic cell hybrids. J. Virol. Methods 1:257–273.
   PubMedGoogle Scholar
• Smith, G. L., M. Mackett, and B. Moss. 1983. Expression of hepatitis B surface antigen by infectious vaccinia virus recombinants. UCLA Symp. Mol. Cell. Biol. New Series 8:543–554.
   Google Scholar
• Thomas, P. S. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77:5201–5205.
   PubMed Web of Science ®Google Scholar
• Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4353.
   PubMed Web of Science ®Google Scholar
• Zoller, M. J., and M. Smith. 1984. Oligonucleotide primers and a single-stranded DNA template. DNA 3:479–488.
   PubMedGoogle Scholar