0
Views
5
CrossRef citations to date
0
Altmetric
Research Article

Diversity among β-Tubulins: A Carboxy-Terminal Domain of Yeast β-Tubulin Is Not Essential In Vivo

&
Pages 2730-2736 | Received 29 Feb 1988, Accepted 04 Apr 1988, Published online: 31 Mar 2023

LITERATURE CITED

  • Andreadis, A., Y. Hsu, G. B. Kohlaw, and P. Schimmel. 1982. Nucleotide sequence of yeast LEU2 shows 5′ noncoding region has sequences cognate to leucine. Cell 31:319–325.
  • Boeke, J. D., F. LaCroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast:5-fluoro-orotic acid selection. Mol. Gen. Genet. 197:345–347.
  • Bond, J. F., J. L. Fridovich-Keil, L. Pillus, R. C. Mulligan, and F. Solomon. 1986. A chicken-yeast chimeric β-tubulin protein is incorporated into mouse microtubules in vivo. Cell 44:461–468.
  • Cleveland, D. W., and K. F. Sullivan. 1985. Molecular biology and genetics of tubulin. Annu. Rev. Biochem. 54:331–365.
  • Fridovich-Keil, J. L., J. F. Bond, and F. Solomon. 1987. Domains of β-tubulin essential for conserved functions in vivo. Mol. Cell. Biol. 7:3792–3798.
  • Fuller, M. T., J. H. Caulton, J. A. Hutchens, T. C. Kaufman, and E. C. Raff. 1987. Genetic analysis of microtubule structure: a p-tubulin mutation causes the formation of aberrant microtubules in vivo and in vitro. J. Cell Biol. 104:385–394.
  • Joshi, H. C., T. J. Yen, and D. W. Cleveland. 1987. In vivo coassembly of a divergent β-tubulin subunit (cβ6) into microtubules of different function. J. Cell Biol. 105:2179–2190.
  • Kemphues, K. J., T. C. Kaufman, R. A. Raff, and E. C. Raff. 1982. The testis-specific β-tubulin subunit in Drosophila mela-nogaster has multiple functions in spermatogenesis. Cell 31:655–670.
  • Kilmartin, J., and A. Adams. 1984. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomycssxxxxxxxxxxxxxxxxxxxxxxxxxxf//ifvkm // es. J. Cell Biol. 98:922–933.
  • Lewis, S. A., W. Gu, and N. J. Cowan. 1987. Free intermingling of mammalian β-tubulin isotypes among functionally distinct microtubules. Cell 49:539–548.
  • Littauer, U. Z., D. Giveo, M. Thierauf, I. Ginzburg, and H. Ponstingl. 1986. Common and distinct tubulin binding sites for microtubule-associated proteins. Proc. Natl. Acad. Sci. USA 83:7162–7166.
  • Lopata, M. A., and D. W. Cleveland. 1987. In vivo microtubules are copolymers of available β-tubulin isotypes: localization of each of six vertebrate β-tubulin isotypes using polyclonal antibodies elicited by synthetic peptide antigens. J. Cell Biol. 105:1707–1720.
  • Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Murphy, D. B., W. A. Grasser, and K. T. Wallis. 1986. Immunofluorescence examination of beta tubulin expression and marginal band formation in developing chicken erythroblasts. J. Cell Biol. 102:628–635.
  • Neff, N. F., J. H. Thomas, P. Grisafi, and D. Botstein. 1983. Isolation of the β-tubulin from yeast and demonstration of its essential function in vivo. Cell 33:211–219.
  • Orr-Weaver, T., J. Szostak, and R. Rothstein. 1981. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. USA 78:6354–6358.
  • Rothstein, R. J. 1983. One-step gene disruption in yeast. Methods Enzymol. 101:202–211.
  • Schatz, P. J., G. E. Georges, F. Solomon, and D. Botstein. 1987. Insertions of up to 17 amino acids into a region of α-tubulin do not disrupt function in vivo. Mol. Cell. Biol. 7:3799–3805.
  • Schatz, P. J., L. Pillus, P. Grisafi, F. Solomon, and D. Botstein. 1986. Two functional α-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. Mol. Cell. Biol. 6:3711–3721.
  • Schatz, P. J., F. Solomon, and D. Botstein. 1986. Genetically essential and nonessential α-tubulin genes specify functionally interchangeable proteins. Mol. Cell. Biol. 6:3722–3733.
  • Sherman, F., G. R. Fink, and J. B. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Sullivan, K. F., and D. W. Cleveland. 1986. Identification of conserved isotype-defining variable region sequences for four vertebrate β-tubulin polypeptide classes. Proc. Natl. Acad. Sci. USA 83:4327–4331.
  • Wang, D., A. Villasante, S. A. Lewis, and N. J. Cowan. 1986. The mammalian β-tubulin repertoire: hematopoietic expression of a novel, heterologous p-tubulin isotype. J. Cell Biol. 103:1903–1910.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.