2
Views
4
CrossRef citations to date
0
Altmetric
Gene Expression

Involvement of Tyrosyl-tRNA Synthetase in Splicing of Group I Introns in Neurospora crassa Mitochondria: Biochemical and Immunochemical Analyses of Splicing Activity

, , , , &
Pages 2089-2104 | Received 23 Nov 1988, Accepted 06 Feb 1989, Published online: 31 Mar 2023

LITERATURE CITED

  • Akins, R. A., and A. M. Lambowitz. 1987. A protein required for splicing group I introns in Neurospora mitochondria is mitochondrial tyrosyl-tRNA synthetase or a derivative thereof. Cell 50:331–345.
  • Bertrand, H., P. Bridge, R. A. Collins, G. Garriga, and A. M. Lambowitz. 1982. RNA splicing in Neurospora mitochondria. Characterization of new nuclear mutants with defects in splicing the mitochondrial large rRNA. Cell 29:517–526.
  • Bradford, M. M.. 1976. A rapid and sensitive method for the quanitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254.
  • Burke, J. M., M. Belfort, T. R. Cech, R. W. Davies, R. J. Schweyen, D. A. Shub, J. W. Szostak, and H. F. Tabak. 1987. Structural conventions for group I introns. Nucleic Acids Res. 15:7217–7221.
  • Cech, T. R., and B. L. Bass. 1986. Biological catalysis by RNA. Annu. Rev. Biochem. 55:599–629.
  • Collins, R. A., and A. M. Lambowitz. 1985. RNA splicing in Neurospora mitochondria. Defective splicing of mitochondrial mRNA precursors in the nuclear mutant cyt18-1. J. Mol. Biol. 184:413–428.
  • Davis, R. H., and F. J. de Serres. 1970. Genetic and microbiological research techniques for Neurospora crassa. Methods Enzymol. 17A:79–143.
  • Deutscher, M. P.. 1984. The eukaryotic aminoacyl-tRNA synthetase complex: suggestions for its structure and function. J. Cell Biol. 99:373–377.
  • Dujardin, G., C. Jacq, and P. P. Slonimski. 1982. Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene. Nature (London) 298:628–632.
  • Ey, P. L., S. J. Prowse, and C. R. Jenkin. 1978. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry 15:429–436.
  • Garriga, G., H. Bertrand, and A. M. Lambowitz. 1984. RNA splicing in Neurospora mitochondria: nuclear mutants defective in both splicing and 3′ end synthesis of the large rRNA. Cell 36:623–634.
  • Garriga, G., and A. M. Lambowitz. 1984. RNA splicing in Neurospora mitochondria: self-splicing of a mitochondrial intron in vitro. Cell 39:631–641.
  • Garriga, G., and A. M. Lambowitz. 1986. Protein-dependent splicing of a group I intron in ribonucleoprotein particles and soluble fractions. Cell 46:669–680.
  • Haenni, A.-L., S. Joshi, and F. Chapeville. 1982. tRNA-like structures in the genomes of RNA viruses. Prog. Nucleic Acid Res. Mol. Biol. 27:85–104.
  • Hanahan, D.. 1985. Techniques for transformation of E. coli, p. 109–135. In D. M. Glover (ed.), DNA cloning: a practical approach, vol. 1. IRL Press Limited, Oxford.
  • Herbert, C. J., M. Labouesse, G. Dujardin, and P. P. Slonimski. 1988. The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing. EMBO J. 7:473–483.
  • Herzog, N. K., and H. R. Bose, Jr.. 1986. Expression of the oncogene of avian reticuloendotheliosis virus in Escherichia coli and identification of the transforming protein in reticuloendotheliosis virus T-transformed cells. Proc. Natl. Acad. Sci. USA 83:812–816.
  • Hill, J., P. McGraw, and A. Tzagoloff. 1985. A mutation in yeast mitochondrial DNA results in a precise excision of the terminal intron of the cytochrome b gene. J. Biol. Chem. 260:3235–3238.
  • Jones, D. H., A. J. McMillan, A. R. Fersht, and G. Winter. 1985. Reversible dissociation of dimeric tyrosyl-tRNA synthetase by mutagenesis at the subunit interface. Biochemistry 24:5852–5857.
  • Kleid, D. G., D. Yansura, B. Small, D. Dowbenko, D. M. Moore, M. J. Grubman, P. D. McKercher, D. O. Morgan, B. H. Robertson, and H. L. Bachrach. 1981. Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine. Science 214:1125–1128.
  • Kreike, J., M. Schulze, T. Pillar, A. Korte, and G. Rodel. 1986. Cloning of a nuclear gene MRS1 involved in the excision of a single group I intron (bI3) from the mitochondrial COB transcript in S. cerevisiae. Curr. Genet. 11:185–191.
  • Labouesse, M., C. J. Herbert, G. Dujardin, and P. P. Slonimski. 1987. Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene. EMBO J. 6:713–721.
  • Laemmli, U. K.. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature (London) 227:680–685.
  • Lambowitz, A. M.. 1979. Preparation and analysis of mitochondrial ribosomes. Methods Enzymol. 59:421–433.
  • Lambowitz, A. M., R. A. Akins, G. Garriga, M. Henderson, A. R. Kubelik, and K. A. Maloney. 1985. Mitochondrial introns and mitochondrial plasmids of Neurospora, p. 237–247. In E. Quagliariello, E. C. Slater, R. Palmieri, C. Saccone, and A. M. Kroon (ed.), Achievements and perspectives of mitochondrial research, vol. 2: biogenesis. Elsevier Science Publishers B.V., Amsterdam.
  • Lazowska, J., C. Jacq, and P. P. Slonimski. 1980. Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron. Cell 22:333–348.
  • Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
  • Mannella, C. A., R. A. Collins, M. R. Green, and A. M. Lambowitz. 1979. Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa. Proc. Natl. Acad. Sci. USA 76:2635–2639.
  • Merril, C. R., D. Goldman, S. A. Sedman, and M. H. Ebert. 1981. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437–1438.
  • O'Farrell, P. Z., H. M. Goodman, and P. H. O'Farrell. 1977. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133–1142.
  • Pittenger, T. H., and D. J. West. 1979. Isolation and characterization of temperature-sensitive respiratory mutants in Neurospora crassa. Genetics 93:539–556.
  • Rietveld, K., C. W. A. Pleij, and L. Bosch. 1983. Three-dimensional models of the tRNA-like 3′ termini of some plant viral RNAs. EMBO J. 2:1079–1085.
  • Schimmel, P.. 1987. Aminoacyl tRNA synthetases: general scheme of structure-function relationships in the polypeptides and recognition of transfer RNAs. Annu. Rev. Biochem. 56:125–158.
  • Spindler, K. R., D. S. E. Rosser, and A. J. Berk. 1984. Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli. J. Virol. 49:132–141.
  • Szostak, J. W.. 1986. Enzymatic activity of the conserved core of a group I self-splicing intron. Nature (London) 322:83–86.
  • Tabak, H. J., and L. A. Grivell. 1986. RNA catalysis in the excision of yeast mitochondrial introns. Trends Genet. 2:51–55.
  • von der Haar, F.. 1973. Affinity elution as a purification method for aminoacyl-tRNA synthetases. Eur. J. Biochem. 34:84–90.
  • Weber, K., and M. Osborn. 1969. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406–4412.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.