The Yeast Gene ERG6 Is Required for Normal Membrane Function but Is Not Essential for Biosynthesis of the Cell-Cycle-Sparking Sterol

LITERATURE CITED

• Aoyama, Y., and Y. Oshida. 1978. The 14a demethylation of lanosterol by a reconstituted cytochrome P-450 system from yeast microsomes. Biochem. Biophys. Res. Commun. 85:28–34.
   PubMed Web of Science ®Google Scholar
• Bard, M.. 1972. Biochemical and genetic aspects of nystatin resistance in Saccharomyces cerevisiae. J. Bacteriol. 111:649–657.
   PubMedGoogle Scholar
• Bard, M., N. D. Lees, L. S. Burrows, and F. W. Kleinhans. 1978. Differences in crystal violet uptake and cation-induced death among yeast sterol mutants. J. Bacteriol. 135:1146–1148.
   PubMed Web of Science ®Google Scholar
• Barton, D. H. R., J. E. T. Corrie, D. A. Widdowson, M. Bard, and R. A. Woods. 1974. Biosynthesis of terpenes and steroids. IX. The sterols of some mutant yeasts and their relationship to the biosynthesis of ergosterol. J. Chem. Soc. Perkin Trans. 1:1326–1333.
   Google Scholar
• Botstein, D., S. C. Falco, S. E. Stewart, M. Brennan, S. Scherrer, D. T. Stinchcomb, K. Struhl, and R. W. Davis. 1979. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–25.
   PubMed Web of Science ®Google Scholar
• Carle, G. F., and M. V. Olson. 1985. An electrophoretic karyotype for yeast. Proc. Natl. Acad. Sci. USA 82:3756–3760.
   PubMed Web of Science ®Google Scholar
• Dahl, C., H. Biemann, and J. Dahl. 1987. A protein kinase antigenically related to pp60v-src possibly involved in yeast cell cycle control: positive in vivo regulation by sterol. Proc. Natl. Acad. Sci. USA 84:4012–4016.
   PubMed Web of Science ®Google Scholar
• Gaber, R. F., L. Mathison, I. Edelman, and M. R. Culbertson. 1982. Frameshift suppression in Saccharomyces cerevisiae. VI. Complete genetic map of twenty-five suppressor genes. Genetics 103:389–407.
   Google Scholar
• Gaber, R. F., C. A. Styles, and G. R. Fink. 1988. TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae. Mol. Cell. Biol. 8:2848–2859.
   PubMed Web of Science ®Google Scholar
• Gollub, E. G., K. Liu, J. Dayan, M. Adlersberg, and D. S. Sprinson. 1977. Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in the cyclization of 2.3-oxidosqualene. J. Biol. Chem. 252:2846–2854.
   PubMed Web of Science ®Google Scholar
• Holmes, D. S., and M. Quigley. 1981. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114:193–197.
   PubMed Web of Science ®Google Scholar
• Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163–168.
   PubMed Web of Science ®Google Scholar
• Lees, N. D., M. Bard, M. D. Kemple, R. A. Haak, and F. W. Kleinhans. 1979. ESR determination of membrane order parameter in yeast sterol mutants. Biochem. Biophys. Acta 553:469–475.
   PubMedGoogle Scholar
• Lees, N. D., M. D. Kemple, R. J. Barbuch, M. A. Smith, and M. Bard. 1984. Differences in membrane order parameter and antibiotic sensitivity in ergosterol-producing strains of Saccharomyces cerevisiae. Biochim. Biophys. Acta 776:105–112.
   Google Scholar
• Lees, N. D., S. L. Lofton, R. A. Woods, and M. Bard. 1980. The effects of varied energy source and detergent on the growth of sterol mutants of Saccharomyces cerevisiae. J. Gen. Microbiol. 118:209–214.
   Google Scholar
• Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor. N.Y.
   Google Scholar
• McCammon, M. T., M. A. Hartmann, C. D. Bottema, and L. W. Parks. 1984. Sterol methylation in Saccharomyces cerevisiae. J. Bacteriol. 157:475–483.
   PubMed Web of Science ®Google Scholar
• Molzahn, S. W., and R. A. Woods. 1972. Polyene resistance and the isolation of sterol mutants in Saccharomyces cerevisiae. J. Gen. Microbiol. 72:339–348.
   PubMedGoogle Scholar
• Pierce, A. M., A. M. Unrau, A. C. Oehlschlager, and R. A. Woods. 1979. Azasterol inhibitors in yeast. Inhibition of the A24-sterol methyltransferase and the 24-methylene sterol A24(28)-reductase in sterol mutants of Saccharomyces cerevisiae. Can. J. Biochem. 57:201–208.
   PubMedGoogle Scholar
• Pinto, W. J., R. Lozano, B. C. Sekula, and W. R. Nes. 1983. Stereochemically distinct roles for sterol in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 112:47–54.
   PubMed Web of Science ®Google Scholar
• Pinto, W. J., and W. R. Nes. 1983. Stereochemical specificity for sterols in Saccharomyces cerevisiae. J. Biol. Chem. 258:4472–4476.
   PubMedGoogle Scholar
• Ramgopal, M., and K. Bloch. 1983. Sterol synergism in yeast. Proc. Natl. Acad. Sci. USA 80:712–715.
   PubMedGoogle Scholar
• Rodriguez, R. J., C. Low, C. D. K. Bottema, and L. W. Parks. 1985. Multiple functions for sterols in Saccharomyces cerevisiae. Biochim. Biophys. Acta 837:336–343.
   PubMed Web of Science ®Google Scholar
• Rodriguez, R. J., and L. W. Parks. 1983. Structural and physiological features of sterols necessary to satisfy bulk membrane and sparking requirements in yeast sterol auxotrophs. Arch. Biochem. Biophys. 225:861–871.
   PubMedGoogle Scholar
• Rose, M. D., P. Novick, J. H. Thomas, D. Botstein, and G. R. Fink. 1987. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60:237–243.
   PubMed Web of Science ®Google Scholar
• Rothstein, R. J.. 1983. One-step disruption in yeast. Methods Enzymol. 101:202–211.
   PubMed Web of Science ®Google Scholar
• Salerno, L. F., and L. W. Parks. 1983. Sterol uptake in the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta 752:240–243.
   Google Scholar
• Sherman, F., G. R. Fink, and J. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor. N.Y.
   Google Scholar
• Taylor, F. L., and L. W. Parks. 1981. An assessment of the specificity of sterol uptake and esterification in Saccharomyces cerevisiae. J. Biol. Chem. 256:13048–13054.
   PubMedGoogle Scholar
• Trueheart, J., J. D. Boeke, and G. R. Fink. 1987. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol. Cell. Biol. 7:2316–2328.
   PubMedGoogle Scholar