2
Views
5
CrossRef citations to date
0
Altmetric
Gene Expression

Development of an In Vitro Transcription System for Neurospora crassa Mitochondrial DNA and Identification of Transcription Initiation Sites

&
Pages 3603-3613 | Received 13 Jan 1989, Accepted 25 May 1989, Published online: 31 Mar 2023

LITERATURE CITED

  • Agsteribbe, E., M. Hartog, and H. de Vries. 1989. Duplication of the tRNAMet and tRNACys genes and of fragments of a gene encoding a subunit of the NADH dehydrogenase complex in Neurospora crassa mitochondrial DNA. Curr. Genet. 15:57–62.
  • Akins, R. A., R. L. Kelley, and A. M. Lambowitz. 1989. Characterization of mutant mitochondrial plasmids of Neurospora spp. that have incorporated tRNAs by reverse transcription. Mol. Cell. Biol. 9:678–691.
  • Akins, R. A., and A. M. Lambowitz. 1984. The [poky] mutant of Neurospora contains a 4-base-pair deletion at the 5′ end of the mitochondrial small rRNA. Proc. Natl. Acad. Sci. USA 81:3791–3795.
  • Baldacci, G., B. Cherif-Zahar, and G. Bernardi. 1984. The initiation of DNA replication in the mitochondrial genome of yeast. EMBO J. 3:2115–2120.
  • Barath, Z., and H. Kuntzel. 1972. Induction of mitochondrial RNA polymerase in Neurospora crassa. Nature (London) New Biol. 240:195–197.
  • Biswas, T. K., T. Baruch, and G. S. Getz. 1987. In vitro characterization of the yeast mitochondrial promoter using single-base substitution mutants. J. Biol. Chem. 262:13690–13696.
  • Bogenhagen, D. F., E. F. Applegate, and B. K. Yoza. 1984. Identification of a promoter for transcription of the heavy strand of human mtDNA: in vitro transcription and deletion mutagenesis. Cell 36:1105–1113.
  • Bogenhagen, D. F., and M. F. Romanelli. 1988. Template sequences required for transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters. Mol. Cell. Biol. 8:2917–2924.
  • Bogenhagen, D. F., and B. K. Yoza. 1986. Accurate in vitro transcription of Xenopus laevis mitochondrial DNA from two bidirectional promoters. Mol. Cell. Biol. 6:2543–2550.
  • Burger, G., M. Helmer Citterich, M. A. Nelson, S. Werner, and G. Macino. 1985. RNA processing in Neurospora crassa mitochondria: transfer RNAs punctuate a large precursor transcript. EMBO J. 4:197–204.
  • Chang, D. D., and D. A. Clayton. 1984. Precise identification of individual promoters for transcription of each strand of human mitochondrial DNA. Cell 36:635–643.
  • Chang, D. D., and D. A. Clayton. 1985. Priming of human mitochondrial DNA replication occurs at the light-strand promoter. Proc. Natl. Acad. Sci. USA 82:351–355.
  • Chang, D. D., and D. A. Clayton. 1986. Precise assignment of the light-strand promoter of mouse mitochondrial DNA: a functional promoter consists of multiple upstream domains. Mol. Cell. Biol. 6:3253–3261.
  • Chang, D. D., and D. A. Clayton. 1986. Precise assignment of the heavy-strand promoter of mouse mitochondrial DNA: cognate start sites are not required for transcriptional initiation. Mol. Cell. Biol. 6:3262–3267.
  • Chang, D. D., W. W. Hauswirth, and D. A. Clayton. 1985. Replication priming and transcription initiate from precisely the same site in mouse mitochondrial DNA. EMBO J. 4:1559–1567.
  • Chen, E. Y., and P. H. Seeburg. 1985. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170.
  • Chow, T. Y.-K., and M. J. Fraser. 1983. Purification and properties of single strand DNA-binding endo-exonuclease of Neurospora crassa. J. Biol. Chem. 258:12010–12018.
  • Christianson, T., J. Edwards, D. Levens, J. Locker, and M. Rabinowitz. 1982. Transcriptional initiation and processing of the small ribosomal RNA of yeast mitochondria. J. Biol. Chem. 257:6494–6500.
  • Christianson, T., and M. Rabinowitz. 1983. Identification of multiple transcriptional initiation sites on the yeast mitochondrial genome by in vitro capping with guanylyltransferase. J. Biol. Chem. 258:14025–14033.
  • Clark-Walker, G. D., C. R. McArthur, and K. S. Sriprakash. 1985. Location of transcriptional control signals and transfer RNA sequences in Torulopsis glabrata mitochondrial DNA. EMBO J. 4:465–473.
  • Collins, R. A.. 1987. Neurospora crassa strain 74-OR23-1A: mitochondrial genes, p. 322–324. In S. J. O'Brien (ed.), Genetic maps, vol. 4. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Collins, R. A., H. Bertrand, R. J. LaPolla, and A. M. Lambowitz. 1979. Mitochondrial ribosome assembly in Neurospora crassa: mutants with defects in mitochondrial ribosome assembly. Mol. Gen. Genet. 177:73–84.
  • Collins, R. A., and A. M. Lambowitz. 1981. Characterization of a variant Neurospora crassa mitochondrial DNA which contains tandem reiterations of a 1.9 kb sequence. Curr. Genet. 4:131–133.
  • Collins, R. A., and A. M. Lambowitz. 1985. RNA splicing in Neurospora mitochondria. Defective splicing of mitochondrial mRNA precursors in the nuclear mutant cyt18-1. J. Mol. Biol. 184:413–428.
  • Davis, R. H., and F. J. De Serres. 1970. Genetic and microbiological research techniques for Neurospora crassa. Methods Enzymol. 17A:79–143.
  • Edwards, J. C., D. Levens, and M. Rabinowitz. 1982. Analysis of transcriptional initiation of yeast mitochondrial DNA in a homologous in vitro transcription system. Cell 31:337–346.
  • Fisher, R. P., J. N. Topper, and D. A. Clayton. 1987. Promoter selection in human mitochondria involves binding of a transcription factor to orientation-independent upstream regulatory elements. Cell 50:247–258.
  • Garriga, G., H. Bertrand, and A. M. Lambowitz. 1984. RNA splicing in Neurospora mitochondria: nuclear mutants defective in both splicing and 3′ end synthesis of the large rRNA. Cell 36:623–634.
  • Heckman, J. E., and U. L. RajBhandary. 1979. Organization of tRNA and rRNA genes in N. crassa mitochondria: intervening sequence in the large rRNA gene and strand distribution of the RNA genes. Cell 17:583–595.
  • Helmer Citterich, M., G. Morelli, and G. Macino. 1983. Nucleotide sequence and intron structure of the apocytochrome b gene of Neurospora crassa mitochondria. EMBO J. 2:1235–1242.
  • Hixson, J. E., and D. A. Clayton. 1985. Initiation of transcription from each of the two human mitochondrial promoters requires unique nucleotides at the transcriptional start sites. Proc. Natl. Acad. Sci. USA 82:2660–2664.
  • Kennell, J. C., and D. R. Pring. 1989. Initiation and processing of atp6, T-urf13 and ORF221 transcripts from mitochondria of T cytoplasm of maize. Mol. Gen. Genet. 216:16–24.
  • Lambowitz, A. M.. 1979. Preparation and analysis of mitochondrial ribosomes. Methods Enzymol. 59:421–433.
  • Lambowitz, A. M., R. J. LaPolla, and R. A. Collins. 1979. Mitochondrial ribosome assembly in Neurospora. Two-dimensional gel analysis of mitochondrial ribosomal proteins. J. Cell Biol. 82:17–31.
  • Levens, D., A. Lustig, and M. Rabinowitz. 1981. Purification of mitochondrial RNA polymerase from Saccharomyces cerevisiae. J. Biol. Chem. 256:1474–1481.
  • Levens, D., B. Ticho, E. Ackerman, and M. Rabinowitz. 1981. Transcriptional initiation and 5′ termini of yeast mitochondrial RNA. J. Biol. Chem. 256:5226–5232.
  • Lis, J. T.. 1980. Fractionation of DNA fragments by polyethylene glycol induced precipitation. Methods Enzymol. 65:347–353.
  • Macino, G., and M. A. Nelson. 1987. Two consensus sequences for RNA processing in Neurospora crassa mitochondria, p. 161–168. In S. Papa, B. Chance, and L. Ernster (ed.). Cytochrome systems. Molecular biology and bioenergetics. Plenum Publishing Corp., New York.
  • Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Mannella, C. A., R. R. Goewert, and A. M. Lambowitz. 1979. Characterization of variant Neurospora crassa mitochondrial DNAs which contain tandem reiterations. Cell 18:1197–1207.
  • Maxam, A. M., and W. Gilbert. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 65:499–560.
  • Mulligan, R. M., G. T. Lau, and V. Walbot. 1988. Numerous transcription initiation sites exist for the maize mitochondrial genes for subunit 9 of the ATP synthase and subunit 3 of cytochrome oxidase. Proc. Natl. Acad. Sci. USA 85:7998–8002.
  • Neupert, W., P. Massinger, and P. Pfaller. 1971. Amino acid incorporation into mitochondrial ribosomes of Neurospora crassa wild type and mi-1 mutant, p. 328–338. In N. K. Boardman, A. W. Linnane, and R. M. Smille (ed.), Autonomy and biogenesis of mitochondria and chloroplasts. North Holland Publishing Co., Amsterdam.
  • Osinga, K. A., M. De Haan, T. Christianson, and H. F. Tabak. 1982. A nonanucleotide sequence involved in promotion of ribosomal RNA synthesis and RNA priming of DNA replication in yeast mitochondria. Nucleic Acids Res. 10:7993–8006.
  • Osinga, K. A., E. De Vries, G. T. J. Van der Horst, and H. F. Tabak. 1984. Initiation of transcription in yeast mitochondria: analysis of origins of replication and of genes coding for a messenger RNA and a transfer RNA. Nucleic Acids Res. 12:1889–1900.
  • Osinga, K. A., R. F. Evers, J. C. Van der Laan, and H. F. Tabak. 1981. A putative precursor for the small ribosomal RNA from mitochondria of Saccharomyces cerevisiae. Nucleic Acids Res. 9:1351–1364.
  • Rifkin, M. R., and D. J. L. Luck. 1971. Defective production of mitochondrial ribosomes in the poky mutant of Neurospora crassa. Proc. Natl. Acad. Sci. USA 68:287–290.
  • Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467.
  • Schinkel, A. H., M. J. A. Groot Koerkamp, M. H. Stuiver, G. T. J. Van der Horst, and H. F. Tabak. 1987. Effect of point mutations on in vitro transcription from the promoter for the large ribosomal RNA gene of yeast mitochondria. Nucleic Acids Res. 15:5597–5612.
  • Walberg, M. W., and D. A. Clayton. 1983. In vitro transcription of human mitochondrial DNA. Identification of specific light strand transcripts from the displacement loop region. J. Biol. Chem. 258:1268–1275.
  • Walseth, T. F., and R. A. Johnson. 1979. The enzymatic preparation of [γ-32P] nucleotide triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP. Biochim. Biophys. Acta 562:11–31.
  • Yin, S., J. Heckman, and U. L. RajBhandary. 1981. Highly conserved GC rich palindromic DNA sequences flank tRNA genes in Neurospora crassa mitochondria. Cell 26:325–332.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.