Isolation, Sequence, and Expression of a Human Keratin K5 Gene: Transcriptional Regulation of Keratins and Insights into Pairwise Control

LITERATURE CITED

• Bader, B. L., T. M. Magin, M. Hatzfeld, and W. W. Franke. 1986. Amino acid sequence and gene organization of cytokeratin no. 19, an exceptional tail-less intermediate filament protein. EMBO J. 5:1865–1875.
   PubMedGoogle Scholar
• Benton, W. D., and R. W. Davis. 1977. Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196:180–182.
   PubMed Web of Science ®Google Scholar
• Blessing, M., H. Zentgraf, and J. L. Jorcano. 1987. Differentially expressed bovine cytokeratin genes. Analysis of gene linkage and evolutionary conservation of 5′-upstream sequences. EMBO J. 6:567–575.
   PubMedGoogle Scholar
• Chen, E. Y., and P. H. Seeburg. 1985. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170.
   PubMedGoogle Scholar
• Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159.
   PubMed Web of Science ®Google Scholar
• Chou, P. Y., and G. D. Fasman. 1978. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47:45–148.
   PubMedGoogle Scholar
• Chou, P. Y., and G. D. Fasman. 1979. Prediction of beta turns. Biophys. J. 26:367–383.
   PubMed Web of Science ®Google Scholar
• Cowan, N. J., P. R. Dobner, E. V. Fuchs, and D. W. Cleveland. 1983. Expression of human α-tubulin genes: surprising interspecies conservation of 3′ untranslated regions. Mol. Cell. Biol. 3:1738–1745.
   PubMed Web of Science ®Google Scholar
• Crewther, W. G., L. M. Dowling, D. A. D. Parry, and P. M. Steinert. 1983. The structure of intermediate filaments. Int. J. Biol. Macromol. 5:267–282.
   Google Scholar
• Dale, B. A., K. A. Holbrook, J. R. Kimball, M. Hoff, and T.-T. Sun. 1985. Expression of epidermal keratins and filaggrin during human fetal skin development. J. Cell Biol. 101:1257–1269.
   PubMedGoogle Scholar
• Darmon, M.. 1985. Coexpression of specific acid and basic cytokeratins in teratocarcinoma-derived fibroblasts treated with 5-azacytidine. Dev. Biol. 110:47–52.
   PubMedGoogle Scholar
• Eckert, R. L., and E. A. Rorke. 1988. The sequence of the human epidermal 58-kd (#5) type II keratin reveals an absence of the 5′ upstream sequence conservation between coexpressed epidermal keratins. DNA 7:337–345.
   PubMedGoogle Scholar
• Eichner, R., P. Bonitz, and T.-T. Sun. 1984. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J. Cell Biol. 98:1388–1396.
   PubMed Web of Science ®Google Scholar
• Eichner, R., T.-T. Sun, and U. Aebi. 1986. The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments. J. Cell Biol. 102:1767–1777.
   PubMed Web of Science ®Google Scholar
• Fuchs, E., S. Coppock, H. Green, and D. Cleveland. 1981. Two distinct classes of keratin genes and their evolutionary significance. Cell 27:75–84.
   PubMed Web of Science ®Google Scholar
• Fuchs, E., and H. Green. 1980. Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19:1033–1042.
   PubMed Web of Science ®Google Scholar
• Fuchs, E., and D. Marchuk. 1983. Type I and type II keratins have evolved from lower eukaryotes to form the epidermal intermediate filaments in mammalian skin. Proc. Natl. Acad. Sci. USA 80:5857–5861.
   PubMedGoogle Scholar
• Fuchs, E., A. L. Tyner, G. J. Giudice, D. Marchuk, A. Ray-Chaudhury, and M. Rosenberg. 1987. The human keratin genes and their differential expression. Curr. Top. Dev. Biol. 22:5–34.
   PubMedGoogle Scholar
• Galup, C., and M. Y. Darmon. 1988. Isolation and characterization of a cDNA clone coding for human epidermal keratin K5. Sequence of the carboxyterminal half of this keratin. J. Invest. Dermatol. 91:39–42.
   PubMedGoogle Scholar
• Geisler, N., E. Kaufmann, and K. Weber. 1985. Antiparallel orientation of the two double-stranded coiled-coils in the tetrameric protofilament unit of intermediate filaments. J. Mol. Biol. 182:173–177.
   PubMedGoogle Scholar
• Giudice, G. J., and E. Fuchs. 1987. The transfection of human epidermal keratin genes into fibroblasts and simple epithelial cells: evidence for inducing a type I keratin by a type II gene. Cell 48:453–463.
   PubMedGoogle Scholar
• Glass, C., and E. Fuchs. 1988. Isolation, sequence, and differential expression of a human K7 gene in simple epithelial cells. J. Cell Biol. 107:1337–1350.
   PubMedGoogle Scholar
• Glass, C., K. H. Kim, and E. Fuchs. 1985. Sequence and expression of a human type II mesothelial keratin. J. Cell Biol. 101:2366–2373.
   PubMedGoogle Scholar
• Graham, F. L., and E. van der Eb. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467.
   PubMed Web of Science ®Google Scholar
• Greenberg, M. E., and E. B. Ziff. 1984. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature (London) 311:433–438.
   PubMed Web of Science ®Google Scholar
• Groudine, M., M. Peretz, and H. Weintraub. 1981. Transcriptional regulation of hemoglobin switching in chicken embryos. Mol. Cell. Biol. 1:281–288.
   PubMed Web of Science ®Google Scholar
• Hanukoglu, I., and E. Fuchs. 1982. The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins. Cell 31:243–252.
   PubMedGoogle Scholar
• Hanukoglu, I., and E. Fuchs. 1983. The cDNA sequence of a type II cytoskeletal keratin reveals constant and variable structural domains among keratins. Cell 33:915–924.
   PubMedGoogle Scholar
• Hanukoglu, I., N. Tanese, and E. Fuchs. 1983. The cDNA sequence of a human cytoplasmic actin: interspecies-divergence in the 3′ untranslated regions. J. Mol. Biol. 163:673–678.
   PubMed Web of Science ®Google Scholar
• Hatzfeld, M., and W. W. Franke. 1985. Pair formation and promiscuity of cytokeratins: formation in vitro of heterotypic complexes and intermediate-sized filaments by homologous and heterologous recombinations of purified polypeptides. J. Cell Biol. 101:1826–1841.
   PubMed Web of Science ®Google Scholar
• Johnson, L., W. Idler, X.-M. Zhou, D. Roop, and P. Steinert. 1985. Structure of a gene for the human epidermal 67-kda keratin. Proc. Natl. Acad. Sci. USA 82:1896–1900.
   PubMedGoogle Scholar
• Jonas, E., T. D. Sargent, and I. B. Dawid. 1985. Epidermal keratin gene expressed in embryos of Xenopus laevis. Proc. Natl. Acad. Sci. USA 82:5413–5417.
   PubMedGoogle Scholar
• Jones, N. C., P. W. J. Rigby, and E. B. Ziff. 1988. Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. Genes Dev. 2:267–281.
   PubMedGoogle Scholar
• Karlsson, S., and A. W. Nienhaus. 1985. Developmental regulation of human globin genes. Annu. Rev. Biochem. 54:1071–1108.
   PubMed Web of Science ®Google Scholar
• Kim, K. H., J. G. Rheinwald, and E. V. Fuchs. 1983. Tissue specificity of epithelial keratins: differential expression of mRNAs from two multigene families. Mol. Cell. Biol. 3:495–502.
   PubMedGoogle Scholar
• Knapp, B., M. Rentrop, J. Schweizer, and H. Winter. 1987. Three cDNA sequences of mouse type I keratins. J. Biol. Chem. 262:938–945.
   PubMed Web of Science ®Google Scholar
• Kopan, R., and E. Fuchs. 1989. A new look into an old problem: keratins as tools to investigate determination, morphogenesis and differentiation in skin. Genes Dev. 3:1–15.
   PubMedGoogle Scholar
• Kopan, R., G. Traska, and E. Fuchs. 1987. Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization. J. Cell Biol. 105:427–440.
   PubMed Web of Science ®Google Scholar
• Krieg, T. M., M. P. Schafer, C. K. Cheng, D. Filpula, P. Flaherty, P. M. Steinert, and D. R. Roop. 1985. Organization of a type I keratin gene. Evidence for evolution of intermediate filaments from a common ancestral gene. J. Biol. Chem. 260:5867–5870.
   PubMedGoogle Scholar
• Kulesh, D. A., G. Cecena, Y. M. Darmon, M. Vasseur, and R. G. Oshima. 1989. Posttranslational regulation of keratins: degradation of mouse and human keratins 18 and 8. Mol. Cell. Biol. 9:1553–1565.
   PubMedGoogle Scholar
• Kulesh, D. A., and R. G. Oshima. 1988. Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. Mol. Cell. Biol. 8:1540–1550.
   PubMedGoogle Scholar
• Land, H., A. C. Chen, J. P. Morgenstern, L. F. Parada, and R. A. Weinberg. 1986. Behavior of myc and ras oncogenes in transformation of rat embryo fibroblasts. Mol. Cell. Biol. 6:1917–1925.
   PubMed Web of Science ®Google Scholar
• Lee, L. D., and H. P. Baden. 1976. Organization of the polypeptide chains in mammalian keratin. Nature (London) 264:377–379.
   PubMedGoogle Scholar
• Lee, W., A. Haslinger, M. Karin, and R. Tjian. 1987. Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40. Nature (London) 325:368–372.
   PubMed Web of Science ®Google Scholar
• Lersch, R., and E. Fuchs. 1988. Sequence and expression of a type II keratin, K5, in human epidermal cells. Mol. Cell. Biol. 8:486–493.
   PubMed Web of Science ®Google Scholar
• Magin, T. M., J. L. Jorcano, and W. W. Franke. 1983. Translational products of mRNAs coding for non-epidermal cytokeratin. EMBO J. 2:1387–1392.
   PubMedGoogle Scholar
• Mansbridge, J. N., and A. M. Knapp. 1987. Changes in keratinocyte maturation during wound healing. J. Invest. Dermatol. 89:253–262.
   PubMed Web of Science ®Google Scholar
• Marchuk, D., S. McCrohon, and E. Fuchs. 1984. Remarkable conservation among intermediate filament genes. Cell 39:491–498.
   PubMedGoogle Scholar
• Marchuk, D., S. McCrohon, and E. Fuchs. 1985. Complete sequence of a type I human keratin gene: presence of enhancerlike elements in the regulatory region of the gene. Proc. Natl. Acad. Sci. USA 82:1609–1613.
   PubMedGoogle Scholar
• McKnight, S. L., and R. Kingsbury. 1982. Transcriptional control signals of a eukaryotic protein-coding gene. Science 217:316–324.
   PubMed Web of Science ®Google Scholar
• McLachlan, A. D.. 1978. Coiled coil formation and sequence regularities in the helical regions of α-keratin. J. Mol. Biol. 124:297–304.
   PubMedGoogle Scholar
• McLachlan, A. D., and M. Stewart. 1975. Tropomyosin coiled-coil interactions: evidence for an unstaggered structure. J. Mol. Biol. 98:293–304.
   PubMed Web of Science ®Google Scholar
• Moll, R., W. Franke, D. Schiller, B. Geiger, and R. Krepler. 1982. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24.
   PubMed Web of Science ®Google Scholar
• Nelson, W., and T.-T. Sun. 1983. The 50- and 58-kdalton keratin classes as molecular markers for stratified squamous epithelia: cell culture studies. J. Cell Biol. 97:244–251.
   PubMed Web of Science ®Google Scholar
• Oshima, R. G., K. Trevor, L. H. Shevinsky, O. A. Ryder, and G. Cecena. 1988. Identification of the gene coding for the Endo B murine cytokeratin and its methylated, stable inactive state in mouse nonepithelial cells. Genes Dev. 2:505–516.
   PubMedGoogle Scholar
• Parker, B. A., and G. R. Stark. 1979. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA. J. Virol. 31:360–369.
   PubMed Web of Science ®Google Scholar
• Parry, D. A. D., A. C. Steven, and P. M. Steinert. 1985. The coiled-coil molecules of intermediate filaments consist of two parallel chains in exact axial register. Biochem. Biophys. Res. Commun. 127:1012–1018.
   PubMedGoogle Scholar
• RayChaudhury, A., D. Marchuk, M. Lindhurst, and E. Fuchs. 1986. Three tightly linked genes encoding human type I keratins: conservation of sequence in the 5′ untranslated leader and 5′ upstream regulatory regions. Mol. Cell. Biol. 6:539–548.
   PubMedGoogle Scholar
• Rieger, M., J. L. Jorcano, and W. W. Franke. 1985. Complete sequence of a bovine type I cytokeratin gene: conserved and variable intron positions in genes of polypeptides of the same cytokeratin subfamily. EMBO J. 4:2261–2267.
   PubMedGoogle Scholar
• Roop, D. R., P. Hawley-Nelson, C. K. Cheng, and S. H. Yuspa. 1983. Keratin gene expression in mouse epidermis and cultued epidermal cells. Proc. Natl. Acad. Sci. USA 80:5857–5861.
   PubMedGoogle Scholar
• Rosenberg, M., A. RayChaudhury, T. B. Shows, M. M. Le Beau, and E. Fuchs. 1988. A group of type I keratin genes on human chromosome 17: characterization and expression. Mol. Cell. Biol. 8:722–736.
   PubMedGoogle Scholar
• Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467.
   PubMed Web of Science ®Google Scholar
• Savtchenko, E. S., I. M. Freedberg, I.-Y. Choi, and M. Blumenberg. 1988. Inactivation of human keratin genes: the spectrum of mutations in the sequence of an acidic keratin pseudogene. Mol. Biol. Evol. 5:97–108.
   PubMedGoogle Scholar
• Schermer, A., S. Galvin, and T.-T. Sun. 1986. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J. Cell Biol. 103:49–62.
   PubMed Web of Science ®Google Scholar
• Southern, E. M.. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.
   PubMed Web of Science ®Google Scholar
• Steinert, P. M., W. W. Idler, and S. B. Zimmerman. 1976. Self-assembly of bovine epidermal keratin filaments in vitro. J. Mol. Biol. 108:547–567.
   PubMed Web of Science ®Google Scholar
• Steinert, P. M., A. C. Steven, and D. R. Roop. 1985. The molecular biology of intermediate filaments. Cell 42:411–419.
   PubMed Web of Science ®Google Scholar
• Stoler, A., R. Kopan, M. Duvic, and E. Fuchs. 1988. The use of monospecific antibodies and cRNA probes reveals abnormal pathways of differentiation in human epidermal diseases. J. Cell Biol. 107:427–446.
   PubMed Web of Science ®Google Scholar
• Sun, T.-T., R. Eichner, A. Schermer, D. Cooper, W. G. Nelson, and R. A. Weiss. 1984. Classification, expression, and possible mechanisms of evolution of mammalian epithelial keratins: a unifying model, p. 169–176. In The Transformed Phenotype. A. Levine, W. Topp, G. van de Woude, and J. D. Watson (ed.), The cancer cell, vol. 1. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Sun, T.-T., and H. Green. 1978. Keratin filaments in cultured human epidermal cells. J. Biol. Chem. 253:2053–2060.
   PubMed Web of Science ®Google Scholar
• Thomas, P.. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77:5201–5205.
   PubMed Web of Science ®Google Scholar
• Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels onto nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 81:4683–4687.
   Google Scholar
• Tyner, A. L., M. J. Eichman, and E. Fuchs. 1985. The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes. Proc. Natl. Acad. Sci. USA 82:4683–4687.
   PubMedGoogle Scholar
• Tyner, A. L., and E. Fuchs. 1986. Evidence for posttranscriptional regulation of the keratins expressed during hyperproliferation and malignant transformation in human epidermis. J. Cell Biol. 103:1945–1955.
   PubMedGoogle Scholar
• Viac, J., M. J. Staquet, J. Thivolet, and C. Goujon. 1980. Experimental production of antibodies against stratum corneum keratin polypeptides. Arch. Dermatol. Res. 267:179–188.
   PubMedGoogle Scholar
• Weiss, R. A., R. Eichner, and T.-T. Sun. 1984. Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes. J. Cell Biol. 98:1397–1406.
   PubMed Web of Science ®Google Scholar
• Wu, Y.-J., L. M. Parker, N. E. Binder, M. A. Beckett, J. H. Sinard, C. T. Griffiths, and J. G. Rheinwald. 1982. The mesothelial keratins: a new family of cytoskeleton proteins identified in cultured mesothelial cells and nonkeratinizing epithelia. Cell 31:693–703.
   PubMed Web of Science ®Google Scholar