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Continuing Education

In Vitro BrdUrd Labeling of Solid Tumors and Immunological Determination of Labeling Index

Pages 47-49 | Published online: 18 Jul 2013

References

  • Taylor JH, Woods PS, Hugher WL: The organization and dupli-cation of chromosomes using tritiated-labeled thymidine. Proc Natl Acad Sci USA 43: 122, 1957.
  • Quastler H, Sherman FG: Cell proliferation kinetics in the intesti-nal epithelium of the mouse. Exp Cell Res 17: 420, 1959.
  • Johnson HA, Bond VP: A method of labeling tissues with tritiated thymidine in vitro and its use in comparing rates of cell prolifera-tion in duct epithelium, fibroadenoma, and carcinoma of human breast. Cancer 14: 639, 1961.
  • Steel GG, Bensted JPM: In vitro studies of cell proliferation in, tumours. I. Critical appraisal of methods and theoretical consider-ations. Europ J Cancer 1: 275, 1965.
  • Rajewsky M: In vitro studies of cell proliferation in tumors. II. Characteristics of standardized in vitro systems for measurement of 3H-thymidine incorporation into tissue explants. Europ J Cancer 1: 281, 1965.
  • Fabrikant JI, Cherry J: The kinetics of cellular proliferation in normal and malignant tissues. V. Analysis of labeling indices and potential tissue doubling times in human tumor cell population. J Surg Oncol 123: 74, 1969.
  • Denekamp J, Kalman RF: In vitro and in vivo labeling of animal tumors with tritiated thymidine. Cell Tissue Kinet 6: 217, 1973.
  • Meyer JS, Connor RE: In vitro labeling of solid tissues with tritiated thymidine for autoradiographic detection of S-phase nuclei. Stain Tech 52: 185, 1977.
  • Sasaki K: Measurement of tritiated thymidine labeling index by incubation in vitro of surgically removed cervical cancer. Gann 68: 307, 1977.
  • Hornstein OP, Heckel A, Schell A: Autoradiographic analysis of the mode of proliferation in the buccal mucosa of rats incubated up to 5 hours. Virchows Arch B Cell Pathol 31: 57, 1979.
  • Chavaudra N, Richard JM, Malaise EP: Labeling index of human squamous cell carcinomas. Comparison of in vivo and in vitro labeling methods. Cell Tissue Kinet 12: 145, 1979.
  • Sasaki K, Takahashi M: Preservation of cell cycle characteristics in solid tumor in vitro. Cancer Res 40: 4810, 1980.
  • Gentili C, Safilippo O, Silvestrini R: Cell proliferation and its relationship to clinical features and relapse in breast cancers. Cancer 48: 974, 1981.
  • Sasaki K, Takahashi M et al: An autoradiographic study of the labeling index of biopsy specimens from gastric cancers. Cancer 54: 1307, 1984.
  • Braunschweiger PG, Poulakos L, Schiffer LM: In vitro labeling and gold activation autoradiography for determination of labeling index and DNA synthesis times of solid tumor. Cancer Res 36: 1748, 1976.
  • Panayi G, Neill W: Scintillation autoradiography. A rapid tech-nique. J Immunol Methods 96: 115, 1972.
  • Gratzner HG, Leif RC: An immunofluorescence method for mon-itoring DNA synthesis by flow cytometry. Cytometry 1: 385, 1981.
  • Gratzner HG: Monoclonal antibody to 5-bromodeoxyuridine: a new reagent for detection of DNA replication. Science 218:474, 1981.
  • Dean PN, Dolbeare F, Gratzner H et al: Cell-cycle analysis using a monoclonal antibody to BrdUrd. Cell Tissue Kinet 17: 427, 1984.
  • Dolbeare F, Gratzner H et al: Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Nat'l Acad Sci USA 80: 5573, 1983.
  • Sasaki K, Murakami T, Ogino T etal: Flow cytometric estimation of cell cycle parameters using monclonal antibody to bromodeoxy-uridine. Cytometry 7: 391, 1986.
  • Sasaki K, Ogino T, Takahashi M: Immunological determination of labeling index on human tumor tissue sections using monoclo-nal anti-BrdUrd antibody. Stain Tech 61: 155, 1986.
  • Dormer P, Brinkmann W etal: Rate and time DNA synthesis of individual Chinese hamster cells. Cell Tissue Kinet 8:399, 1975.
  • Smallwood JA, Cooper A, Taylor I: The errors of thymidine labeling in breast cancer. Clin Oncol 9: 331, 1983.
  • Lambert M: Tritiated thymidine labeling in vitro of human cancer of the breast: Counting error and sampling error. Eur J Cancer Clin Oncol 22: 781, 1986.
  • Morstyn G, Hsu S-M, Kinsella T et al: Bromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody. J Clin Invest 72: 1844, 1983.

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