Effect of Microwave Pretreatment on Proliferating Cell Nuclear Antigen (PCNA) Immunolocalization in Paraffin Sections

References

• Brooks DJ, Garewal HS: Measures of tumor proliferative activ-ity. Int J Clin Lab Res 22:196–200, 1992
   PubMedGoogle Scholar
• Luque EH, Muñoz de Toro de Luque M, Romero Acuña L et al: Marcadores moleculares para predecir el pronóstico del cancer de mama. Medicina (Bs As) 53:151–166, 1993
   PubMedGoogle Scholar
• Yu CC-W, Hall PA, Fletcher CDM et al: Haemangiopericy-tomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA). Histopathology 19:29–33, 1991
   PubMed Web of Science ®Google Scholar
• Jain S, Filipe MI, Hall PA et al: Prognostic value of proliferat-ing cell nuclear antigen in gastric carcinomas. J Clin Pathol 44: 655–659, 1991
   PubMed Web of Science ®Google Scholar
• Woods AL, Hall PA, Shepherd NA et al: The assessment of the proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis. Histopathology 19:21–27, 1991
   PubMed Web of Science ®Google Scholar
• Takahashi H, Stnitton GM, Parsons PG: Determination of pro-liferating fractions in malignant melanomas by anti-PCNA/cy-clin monoclonal antibody. Histopathology 18:221–227, 1991
   PubMed Web of Science ®Google Scholar
• Visakorpi T, Kallioniemi OP, Heilckinen A et al: Small sub-group of aggressive, highly proliferative prostatic carcinomas defined by p53 accumulation. J Nail Cancer Inst 84:883–887, 1992
   PubMed Web of Science ®Google Scholar
• Tahan SR, Neuberg DS, Dieffenbach A et al: Prediction of early relapse and shortened survival in patients with breast cancer by proliferating cell nuclear antigen score. Cancer 71:3552–3559, 1993
   PubMed Web of Science ®Google Scholar
• Al-Sheneber IF, Shibata HR, Sampalis J et al: Prognostic significance of proliferating cell nuclear antigen expression in colorectal cancer. Cancer 71:1954-1959, 1993
   PubMed Web of Science ®Google Scholar
• Cohen MB, Waldman FM, Carroll PR et al: Comparison of five histopathologic methods to assess cellular proliferation in tran-sitional cell carcinoma of the urinary bladder. Hum Pathol 24:772–778, 1993
   PubMed Web of Science ®Google Scholar
• Kamel OW, LeBrun DP, Davis RE et al: Growth fraction esti-mation of malignant lymphomas in formalin-fixed paraffin em-bedded tissue using anti-PCNA/Cyclin 19A2: Correlation with Ki-67 labeling. Am J Pathol 138:1471–1477, 1991
   PubMed Web of Science ®Google Scholar
• Garcia RL, Coltrera MD, Gown AM: Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Am J Pathol 134:733–739, 1989
   PubMed Web of Science ®Google Scholar
• Celis JE, Bravo R, Larsen PM et al: Cyclin: a nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cells. Leukemia Res 8:143–157, 1984
   PubMed Web of Science ®Google Scholar
• Galand P, Degraef C: Cyclin/PCNA immunostaining as an al-ternative to tritiated thymidine pulse labeling for marking S phase cells in paraffin sections from animal and human tissues. Cell Tiss Kinet 22:383–392, 1989
   PubMedGoogle Scholar
• Hall PA, Levison DA, Woods AL et al: Proliferating cell nu-clear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated ex-pression in some neoplasms. J Pathol 162:285–294, 1990
   PubMed Web of Science ®Google Scholar
• Gasparini G, Meli S, Pozza Fetal: PC-10 antibody to proliferat-ing cell nuclear antigen (PCNA) is not related to prognosis in human breast carcinoma. Growth Regulat 2:145–150, 1992
   PubMedGoogle Scholar
• Noguchi M, Thomas M, Kitagawa H et al: The prognostic significance of proliferating cell nuclear antigen in breast can-cer: correlation with DNA ploidy,, c-erb-2 expression, histo-pathology,, lymph node metastases and patient survival. Int J Oncol 2:985–989, 1993
   PubMed Web of Science ®Google Scholar
• Shi S-R, Key ME, Kalra KL: Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heat-ing of tissue sections. J Histochem Cytochem 39:741–748, 1991
   PubMed Web of Science ®Google Scholar
• Greenwell A, Foley JF, Maronpot RR: An enhanced method for immunohistochemical staining of proliferating cell nuclear antigen in archival rodent tissues. Cancer Lett 59:251–256, 1991
   PubMed Web of Science ®Google Scholar
• Golick ML, Rice M: Optimum staining of PCNA in paraffin sections is dependent on fixation, drying, and intensification. J Histotechnol 15:39–41, 1992
   Web of Science ®Google Scholar
• Ciocca DR, Luque EH: Immunological evidence for the iden-tity between the hsp27 estrogen-regulated heat shock protein and the p29 estrogen receptor-associated protein in breast and endometrial cancer. Breast Cancer Res Treat 20:33–42, 1991
   PubMed Web of Science ®Google Scholar
• Henke R-P, Kruger E, Ayhan N et al: Numerical chromoso-mal aberrations in prostate cancer: correlation with morphol-ogy and cell kinetics. Virchows Archiv A Pathol Anat 422:61–66, 1993
   Google Scholar
• Snedecor GW, Cochran WG: Statistical Methods. Iowa State University Press, Ames, 1974
   Google Scholar
• Pollard JH: Handbook of Numerical and Statistical Tech-niques. Cambridge University Press, London, 1979
   Google Scholar
• Siegel S: Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill, New York, 1956
   Google Scholar
• Celis JE, Madsen P, Celis A et al: Cyclin (PCNA, auxiliary protein of DNA polymerase delta) is a central component of the pathway(s) leading to DNA replication and cell division. FEBS Lett 220:1–7, 1987
   PubMed Web of Science ®Google Scholar
• Dietrich DR: Toxicological and pathological applications of proliferating cell nuclear antigen (PCNA), a novel endoge-nous marker for cell proliferation. Crit Rev Toxicol 23:77–109, 1993
   PubMed Web of Science ®Google Scholar
• Wynnchuck M: Minimizing artifacts in tissue processing: Part 2. Theory of tissue processing. J Histotechnol 16:71–73, 1993
   Web of Science ®Google Scholar
• Bruno S, Gorczyca W, Darzynkiewicz Z: Effect of ionic strength in immunocytochemical detection of the proliferation associated nuclear antigens p120, PCNA, and the protein reacting with Ki-67 antibody. Cytometry 13:496–501, 1992
   PubMedGoogle Scholar
• Bravo R, Macdonald-Bravo H: Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cy-cle: association with DNA replication sites. J Cell Biol 105: 1549–1554, 1987
   PubMed Web of Science ®Google Scholar
• Nakane PK, Moriuchi T, Koji T et al: Proliferating cell nu-clear antigen (PCNA/cyclin): review and some new findings. Acta Histochem Cytochem 22:105–116, 1989
   Web of Science ®Google Scholar
• Landberg G, Roos G: Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analy-sis. Cancer Res 51:4570–4574, 1991
   PubMed Web of Science ®Google Scholar
• Elias JM, Qiao L, Heimann A et al: Paraffin embedded breast carcinomas for the immunohistochemical study of prognostic factors. J Histotechnol 15:315–320, 1992
   Web of Science ®Google Scholar
• McCormick D, Hall PA: The complexities of proliferating cell nuclear antigen. Histopathology 21:591–594, 1992
   PubMed Web of Science ®Google Scholar
• Cattoretti G, Becker MHG, Key G et al: Monoclonal antibod-ies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 168:357–363, 1992
   PubMed Web of Science ®Google Scholar