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Original Articles

Conversion of Capsular Polysaccharide, Involved in Pellicle Formation, to Extracellular Polysaccharide by galE Deletion in Acetobacter tropicalis

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Pages 2536-2539 | Received 15 Mar 2006, Accepted 11 Jun 2006, Published online: 22 May 2014

  • 1) Deeraksa, A., Moonmangmee, S., Toyama, H., Yamada, M., Adachi, O., and Matsushita, K., Characterization and spontaneous mutation of a novel gene, polE, involved in pellicle formation in Acetobacter tropicalis SKU1100. Microbiology, 151, 4111–4120 (2005).
  • 2) Metzger, M., Bellemann, P., Bugert, P., and Geider, K., Genetics of galactose metabolism of Erwinia amylovora and its influence on polysaccharide synthesis and virulence of the fire blight pathogen. J. Bacteriol., 176, 450–459 (1994).
  • 3) Pierson, D. E., and Carlson, S., Identification of the galE gene and a galE homolog and characterization of their roles in the biosynthesis of lipopolysaccharide in a serotype O:8 strain of Yersinia enterocolitica. J. Bacteriol., 178, 5916–5924 (1996).
  • 4) Boels, I. C., Ramos, A., Kleerebezem, M., and De Vos, W. M., Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis. Appl. Environ. Microbiol., 67, 3033–3040 (2001).
  • 5) Yoshida, T., Ayabe, Y., Yasunaga, M., Usami, Y., Habe, H., Nojiri, H., and Omori, T., Genes involved in synthesis of the exopolysaccharide methanolan by the obligate methylotroph Methylobacillus sp. strain 12S. Microbilogy, 149, 431–444 (2003).
  • 6) Marx, C. J., and Lidstrom, M. E., Development of improved versatile broad-host-range vectors use in methylotrophs and other Gram-negative bacteria. Microbiology, 147, 2065–2075 (2001).
  • 7) Moonmangmee, S., Kawabata, K., Tanaka, S., Toyama, H., Adachi, O., and Matsushita, K., A novel polysaccharide involved in the pellicle formation of Acetobacter aceti. J. Biosci. Bioeng., 93, 192–200 (2002a).
  • 8) Moonmangmee, S., Toyama, H., Adachi, O., Theeragool, G., Lotong, N., and Matsushita, K., Purification and characterization of a novel polysaccharide involved in the pellicle produced by a thermotolerant Acetobacter strain. Biosci. Biotechnol. Biochem., 66, 777–783 (2002b).
  • 9) Dubois, M., Gilles, K. A., Hamilton, J. K., Robers, P. A., and Smith, F., Colorimetric method for determination of sugars and related substances. Anal. Chem., 28, 350–356 (1956).
  • 10) Dulley, J. R., and Grieve, P. A., A simple technique for eliminating interference by detergents in Lowry’s method of protein determination. Anal. Biochem., 64, 136–141 (1975).

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