564
Views
33
CrossRef citations to date
0
Altmetric
Original Articles

Molecular Cloning, Expression, and Characterization of a β-Agarase Gene, agaD, from a Marine Bacterium, Vibrio sp. Strain PO-303

, &
Pages 38-46 | Received 31 May 2006, Accepted 18 Sep 2006, Published online: 22 May 2014

  • 1) Duckworth, M., and Yaphe, W., Structure of agar. I. Fractionation of a complex mixture of polysaccharides. Carbohydr. Res., 16, 189–197 (1971).
  • 2) Araki, T., Lu, Z., and Morishita, T., Optimization of parameters for isolation of protoplasts from Gracilaria verrucosa (Rhodophyta). J. Mar. Biotechnol., 6, 193–197 (1998).
  • 3) Yoshizawa, Y., Ametani, A., Tsunehiro, J., Nomura, K., Itoh, M., Fukui, F., and Kaminogawa, S., Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structure-function relationships and improved solubility. Biosci. Biotechnol. Biochem., 59, 1933–1937 (1995).
  • 4) Kobayashi, R., Takisada, M., Suzuki, T., Kirimura, K., and Usami, S., Neoagarobiose as a novel moisturizer with whitening effect. Biosci. Biotechnol. Biochem., 61, 162–163 (1997).
  • 5) Araki, T., Hayakawa, M., Lu, Z., Karita, S., and Morishita, T., Purification and characterization of agarases from a marine bacterium, Vibrio sp. PO-303. J. Mar. Biotechnol., 6, 260–265 (1998).
  • 6) Saito, H., and Miura, K., Preparation of transforming deoxyribonucleicacid by phenol-treatment. Biochim. Biophys. Acta, 72, 619–629 (1963).
  • 7) Somogyi, M., Notes on sugar determination. J. Biol. Chem., 195, 19–23 (1952).
  • 8) Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248–254 (1976).
  • 9) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685 (1970).
  • 10) Su, J., and Hassid, W. Z., Carbohydrates and nucleotides in the red alga, Porphyra perforate. I. Isolation and identification of carbohydrates. Biochemistry, 1, 468–474 (1962).
  • 11) Bailey, R. W., and Bourne, E. J., Colour reactions given by sugars and diphenylamine-aniline spray reagents on paper chromatograms. J. Chromatogr., 4, 206–213 (1960).
  • 12) Shine, J., and Dalgarno, L., Determinant of cistron specificity on bacterial ribosomes. Nature, 254, 34–38 (1975).
  • 13) Rosenberg, M., and Court, D., Regulatory sequences involved in the promotion and termination of RNA transcription. Annu. Rev. Genet., 13, 319–353 (1979).
  • 14) Sugimoto, N., Nakano, S., Katoh, M., Matsumura, A., Nakamura, H., Ohmichi, T., Yoneyama, M., and Sasaki, M., Thermodynamic parameters to predict stability of RNA/DNA hybrid duplexes. Biochem., 34, 11211–11216 (1995).
  • 15) Ha, J. C., Kim, G. T., Kim, S. K., Oh, T. K., Yu, J. H., and Kong, I. L., β-Agarase from Pseudomonas sp. W7: purification of the recombinant enzyme from Escherichia coli and the effects of salt on its activity. Biotechnol. Appl. Biochem., 26, 1–6 (1997).
  • 16) Kang, N. Y., Cho, Y. L., Cho, Y. S., Kim, B. K., Jeon, B. S., Cha, J. Y., Kim, C. H., and Lee, Y. C., Cloning, expression and characterization of a β-agarase gene from a marine bacterium, Pseudomonas sp. SK38. Biotechnol. Lett., 25, 1165–1170 (2003).
  • 17) Vera, J., Alvarez, R., Murano, E., Slebe, J. C., and Leon, O., Identification of a marine agarolytic Pseudoalteromonas isolate and characterization of its extracellular agarase. Appl. Environ. Microbiol., 64, 4378–4383 (1998).
  • 18) Potin, P., Richard, C., Rochas, C., and Kloareg, B., Purification and characterization of the α-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B. Eur. J. Biochem., 214, 599–607 (1993).
  • 19) Ohta, Y., Nogi, Y., Miyazaki, M., Li, Z., Hatada, Y., Ito, S., and Horikoshi, K., Enzymatic properties and nucleotide and amino acid sequence of a thermostable β-agarase from the novel marine isolate, JAMB-A94. Biosci. Biotechnol. Biochem., 68, 1073–1081 (2004).
  • 20) Ohta, Y., Hatada, Y., Nogi, Y., Miyazaki, M., Li, Z., Akita, M., Hidaka, Y., Goda, S., Ito, S., and Horikoshi, K., Enzymatic properties and nucleotide and amino acid sequence of a thermostable β-agarase from a novel species of deep-sea Microbulbifer. Appl. Microbiol. Biotechnol., 64, 505–514 (2004).
  • 21) Ohta, Y., Hatada, Y., Nogi, Y., Li, Z., Ito, S., and Horikoshi, K., Cloning, expression, and characterization of a glycoside hydrolase family 86 β-agarase from a deep-sea Microbulfifer-like isolate. Appl. Microbiol. Biotechnol., 66, 266–275 (2004).
  • 22) Ohta, Y., Hatada, Y., Ito, S., and Horikoshi, K., High-level expression of a neoagarobiose-producing β-agarase gene from Agarivorans sp. JAMB-A11 in Bacillus subtilis and enzymatic properties of the recombinant enzyme. Biotechnol. Appl. Biochem., 41, 183–191 (2005).
  • 23) Sugano, Y., Matsumoto, T., Kodama, H., and Noma, M., Cloning and sequencing of agaA, a unique agarase 0107 gene from a marine bacterium, Vibrio sp. strain JT0107. Appl. Environ. Microbiol., 59, 3750–3756 (1993).
  • 24) Sugano, Y., Matsumoto, T., and Noma, M., Sequence analysis of the agaB gene encoding a new β-agarase from Vibrio sp. strain JT0107. Biochem. Biophys. Acta, 1218, 105–108 (1994).
  • 25) Ohta, Y., Hatada, Y., Miyazaki, M., Nogi, Y., Ito, S., and Horikoshi, K., Purification and characterization of a novel α-agarase from a Thalassomonas sp. Curr. Microbiol., 50, 212–216 (2004).
  • 26) Allouch, J., Jam, M., Helbert, W., Barbeyron, T., Kloareg, B., Henrissat, B., and Czjzek, M., The three-dimensional structures of two β-agarases. J. Biol. Chem., 278, 47171–47180 (2003).
  • 27) Michel, G., Chantalat, L., Duee, E., Barbeyron, T., Henrissat, B., Kloareg, B., and Dideberg, O., The κ-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of clan-B glycoside hydrolases. Structure, 9, 513–525 (2001).
  • 28) Hahn, M., Olsen, O., Politz, O., Borriss, R., and Heinemann, U., Crystal structure and site-directed mutagenesis of Bacillus macerans endo-1,3-1,4-β-glucanase. J. Biol. Chem., 270, 3081–3088 (1995).
  • 29) Juncosa, M., Pons, J., Dot, T., Querol, E., and Planas, A., Identification of active site carboxylic residues in Bacillus licheniformis 1,3-1,4-β-D-glucan 4-glucanohydrolase by site-directed mutagenesis. J. Biol. Chem., 269, 14530–14535 (1994).
  • 30) McCarter, J. D., and Withers, S. G., Mechanisms of enzymatic glycoside hydrolysis. Curr. Opin. Struct. Biol., 4, 885–892 (1994).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.