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Original Articles

Non-Denaturing Fluorescence in Situ Hybridization to Find Replication Origins in a Specific Genome Region on the DNA Fiber

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Pages 627-632 | Received 24 Nov 2006, Accepted 20 Dec 2006, Published online: 22 May 2014

  • 1) Kelly, T. J., and Brown, G. W., Regulation of chromosome replication. Annu. Rev. Biochem., 69, 829–880 (2000).
  • 2) Newlon, C. S., and Theis, J. F., The structure and function of yeast ARS elements. Curr. Opin. Genet. Dev., 3, 752–758 (1993).
  • 3) Yamashita, M., Hori, Y., Shinomiya, T., Obuse, C., Tsurimoto, T., Yoshikawa, H., and Shirahige, K., The efficiency and timing of initiation of replication of multiple replicons of Saccharomyces cerevisiae chromosome VI. Genes Cells, 2, 655–665 (1997).
  • 4) Cimbora, D. M., and Groudine, M., The control of mammalian DNA replication: a brief history of space and timing. Cell, 104, 643–646 (2001).
  • 5) Schwaiger, M., and Schubeler, D., A question of timing: emerging links between transcription and replication. Curr. Opin. Genet. Dev., 16, 177–183 (2006).
  • 6) Takebayashi, S.-I., Sugimura, K., Saito, T., Sato, C., Fukushima, Y., Taguchi, H., and Okumura, K., Regulation of replication at the R/G chromosomal band boundary and pericentromeric heterochromatin of mammalian cells. Exp. Cell Res., 304, 162–174 (2005).
  • 7) Selig, S., Okumura, K., Ward, D. C., and Cedar, H., Delineation of DNA replication time zones by fluorescence in situ hybridization. EMBO J., 11, 1217–1225 (1992).
  • 8) Kagotani, K., Takebayashi, S., Kohda, A., Taguchi, H., Paulsen, M., Walter, J., Reik, W., and Okumura, K., Replication timing properties within the mouse distal chromosome 7 imprinting cluster. Biosci. Biotechnol. Biochem., 66, 1046–1051 (2002).
  • 9) Jackson, D. A., and Pombo, A., Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells. J. Cell Biol., 140, 1285–1295 (1998).
  • 10) Takebayashi, S.-I., Manders, E. M., Kimura, H., Taguchi, H., and Okumura, K., Mapping sites where replication initiates in mammalian cells using DNA fibers. Exp. Cell Res., 271, 263–268 (2001).
  • 11) Anglana, M., Apiou, F., Bensimon, A., and Debatisse, M., Dynamics of DNA replication in mammalian somatic cells: nucleotide pool modulates origin choice and interorigin spacing. Cell, 114, 385–394 (2003).
  • 12) Lebofsky, R., and Bensimon, A., DNA replication origin plasticity and perturbed fork progression in human inverted repeats. Mol. Cell. Biol., 25, 6789–6797 (2005).
  • 13) Norio, P., Kosiyatrakul, S., Yang, Q., Guan, Z., Brown, N. M., Thomas, S., Riblet, R., and Schildkraut, C. L., Progressive activation of DNA replication initiation in large domains of the immunoglobulin heavy chain locus during B cell development. Mol. Cell, 20, 575–587 (2005).
  • 14) Nogami, M., Nogami, O., Kagotani, K., Okumura, M., Taguchi, H., Ikemura, T., and Okumura, K., Intranuclear arrangement of human chromosome 12 correlates to large-scale replication domains. Chromosoma, 108, 514–522 (2000).
  • 15) Burhans, W. C., Vassilev, L. T., Wu, J., Sogo, J. M., Nallaseth, F. S., and DePamphilis, M. L., Emetine allows identification of origins of mammalian DNA replication by imbalanced DNA synthesis, not through conservative nucleosome segregation. EMBO J., 10, 4351–4360 (1991).
  • 16) Tenzen, T., Yamagata, T., Fukagawa, T., Sugaya, K., Ando, A., Inoko, H., Gojobori, T., Fujiyama, A., Okumura, K., and Ikemura, T., Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex. Mol. Cell. Biol., 17, 4043–4050 (1997).
  • 17) Nogami, M., Kohda, A., Taguchi, H., Nakao, M., Ikemura, T., and Okumura, K., Relative locations of the centromere and imprinted SNRPN gene within chromosome 15 territories during the cell cycle in HL60 cells. J. Cell Sci., 113, 2157–2165 (2000).
  • 18) Koberna, K., Stanek, D., Malinsky, J., Eltsov, M., Pliss, A., Ctrnacta, V., Cermanova, S., and Raska, I., Nuclear organization studied with the help of a hypotonic shift: its use permits hydrophilic molecules to enter into living cells. Chromosoma, 108, 325–335 (1999).

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