106
Views
4
CrossRef citations to date
0
Altmetric
Original Articles

High Expression of the Second Lysine Decarboxylase Gene, ldc, in Escherichia coli WC196 Due to the Recognition of the Stop Codon (TAG), at a Position Which Corresponds to the 33th Amino Acid Residue of σ38, as a Serine Residue by the Amber Suppressor, supD

, &
Pages 2012-2017 | Received 06 Apr 2000, Accepted 01 May 2000, Published online: 22 May 2014

  • 1) Kikuchi, Y., Kojima, H., Tanaka, T., Takatsuka, Y., and Kamio, Y., Characterization of the second lysine decarboxylase isolated from Escherichia coli. J. Bacteriol., 179, 4486-4492 (1997).
  • 2) Tanaka, K., Takayanagi, Y., Fujita, N., Ishihama, A., and Takahashi, H., Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, σ38, is a second principal sigma factor of RNA polymerase in stationary phase Escherichia coli. Proc. Natl. Acad. Sci. U.S.A., 90, 3511-3515 (1993).
  • 3) Kikuchi, Y., Kurahashi, O., Nagano, T., and Kamio, Y., RpoS-dependent expression of the second lysine decarboxylase gene in Escherichia coli. Biosci. Biotechnol. Biochem., 62, 1267-1270 (1998).
  • 4) Mulvery, M. R., Switala, J., Borys, A., and Loewen, P. C., Regulation of transcription of katE and katF in Escherichia coli. J. Bacteriol., 172, 6173-6720 (1990).
  • 5) Maurizi, M. R., Clark, W. P., Katayama, Y., Rudikoff, S., Pumphrey, J., Bowers, B., and Gottesman, S., Sequence and structure of ClpP, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli. J. Biol. Chem., 265, 12536-12545 (1990).
  • 6) Yoo, S. J., Seol, J. H., Kang, M. S., Ha, D. B., and Chung, C. H., clpX encoding an alternative ATP-binding subunit of protease Ti (Clp) can be expressed independently from clpP in Escherichia coli. Biochem. Biophys. Res. Commun., 203, 798-804 (1994).
  • 7) Zhou, Y. and Gottesman, S., Regulation of proteolysis of the stationary-phase sigma factor RpoS. J. Bacteriol., 180, 1154-1158 (1998).
  • 8) Olsen, A., Arnqvist, A., Hammar, M., Sukupolvi, S., and Normark, S., The RpoS sigma factor relieves H-NS-mediated transcriptional repression of csgA, the subunit gene of fibronectin-binding curli in Escherichia coli. Mol. Microbiol., 7, 523-536 (1993).
  • 9) Muffler, A., Traulsen, D. D., Fischer, D., Lange, R., and Hengge-Aronis, R., The RNA-binding protein HF-I plays a global regulatory role which is largely, but not exclusively, due to its role in expression of the σS subunit of RNA polymerase in Escherichia coli. J. Bacteriol., 179, 297-300 (1997).
  • 10) Ivanova, A., Renshaw, Guntaka, R. V., and Eisenstark, A., DNA base sequence variability in katF (putative sigma factor) gene of Escherichia coli. Nucleic Acids Res., 20, 5479-5480 (1992).
  • 11) Strom, A. R. and Kaasen, I., Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. Mol. Microbiol., 8, 205-210 (1993).
  • 12) Styrvold, O. B. and Strom, A. R., Synthesis, accumulation and excretion of trehalose in osmotically stressed Escherichia coli K-12 strains: influence of amber suppressors and function of the periplasmic trehalase. J. Bacteriol., 173, 1188-1192 (1991).
  • 13) Tate, W. P. and Mannering, S. A., Three, four or more: the translational stop signal at length. Mol. Microbiol., 21, 213-219 (1996).
  • 14) Schagger, H. and von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem., 166, 368-379 (1987).
  • 15) Van Dyke, M. W., Sirito, M., and Sawadogo, M., Single-step purification of bacterially expressed polypeptides containing an oligo-histidine domain. Gene, 111, 99-104 (1992).
  • 16) Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K., and Pease, L. R., Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene, 77, 61-68 (1989).
  • 17) Bachmann, B. J., Linkage map of Escherichia coli K-12, edition 8. Microbiol. Rev., 54, 130-197 (1990).
  • 18) Amann, E., Ochs, B., and Abel, K. J., Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene, 69, 301-315 (1998).
  • 19) Vieira, J. and Messing, J., The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 19, 259-268 (1982).
  • 20) Bernardi, A. and Bernardi, F., Complete sequence of pSC101. Nucleic Acids Res., 12, 9415-9426 (1984).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.