194
Views
0
CrossRef citations to date
0
Altmetric
Original Articles

Efficient Selection for Thermostable Protease in Thermus thermophilus

, , , &
Pages 899-902 | Received 26 Oct 1999, Accepted 13 Dec 1999, Published online: 22 May 2014

  • 1) Kotsuka, T., Akanuma, S., Tomuro, M., Yamagishi, A., and Oshima, T., Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method. J. Bacteriol., 178, 723-727 (1996).
  • 2) Akanuma, S., Yamagishi, A., Tanaka, N., and Oshima, T., Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution. Protein Sci., 7, 698-705 (1998).
  • 3) Sakaki, Y. and Oshima, T., Isolation and characterization of a bacteriophage infectious to an extreme thermophile, Thermus thermophilus HB8. J. Virol., 15, 1449-1453 (1975).
  • 4) Mather, M. W. and Fee, J. A., Development of plasmid cloning vectors for Thermus thermophilus HB8: expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene. Appl. Environ. Microbiol., 58, 421-425 (1992).
  • 5) Maseda, H. and Hoshino, T., Development of expression vectors for Thermus thermophilus. J. Ferment. Bioeng., 86, 121-124 (1998).
  • 6) Zhao, H., Giver, L., Shao, Z., Affholter, J. A., and Arnold, F. H., Molecular evolution by staggered extension process (StEP) in vitro recombination. Nature Biotech., 16, 258-261 (1998).
  • 7) Zhao, H. and Arnold, F. H., Directed evolution converts subtilisin E into a functional equivalent of thermitase. Protein Eng., 12, 47-53 (1999).
  • 8) Takagi, H., Suzumura, A., Hoshino, T., and Nakamori, S., Gene expression of Bacillus subtilis subtilisin E in Thermus thermophilus. J. Ind. Microbiol. Biotech., 23, 214-217 (1999).
  • 9) Hidaka, Y., Hasegawa, M., Nakahara, T., and Hoshino, T., The entire population of Thermus thermophilus cells is always competent at any growth phase. Biosci. Biotechnol. Biochem., 58, 1338-1339 (1994).
  • 10) Koyama, Y., Hoshino, T., and Tomizuka, N., Genetic transformation of the extreme thermophile Thermus thermophilus and other Thermus spp. J. Bacteriol., 166, 338-340 (1986).
  • . 1992. p. 143- 149.
  • 12) Matsuzawa, H., Hamaoki, M., and Ohta, T., Production of thermophilic extracellular proteases (aqualysin I and II) by Thermus aquaticus YT-1, an extreme thermophile. Agric. Biol. Chem., 47, 25-28 (1983).
  • 13) Matsuzawa, H., Tokugawa, K., Hamaoki, M., Mizoguchi, M., Taguchi, H., Terada, I., Kwon, S.-T., and Ohta, T., Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1. Eur. J. Biochem., 171, 441-447 (1988).
  • 14) Touhara, N., Taguchi, H., Koyama, Y., Ohta, T., and Matsuzawa, H., Production and extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) in a host-vector system for Thermus thermophilus. Appl. Environ. Microbiol., 57, 3385-3387 (1991).
  • 15) Hoshino, T., Maseda, H., and Nakahara, T., Plasmid marker transformation in Thermus thermophilus. J. Ferment. Bioeng., 76, 276-279 (1993).
  • 16) Venekei, I., Hedstrom, L., and Rutter, W. J., A rapid and effective procedure for screening protease mutants. Protein Eng., 9, 85-93 (1996).
  • 17) Terada, I., Kwon, S.-T., Miyata, Y., Matsuzawa, H., and Ohta, T., Unique precursor structure of an extracellular protease, aqualysin I, with NH2- and COOH-terminal prosequences and its processing in Escherichia coli. J. Biol. Chem., 265, 6576-6581 (1990).
  • 18) Maseda, H. and Hoshino, T., Screening and analysis of DNA fragments that show promoter activities in Thermus thermophilus. FEMS Microbiol. Lett., 128, 127-134 (1995).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.