158
Views
7
CrossRef citations to date
0
Altmetric
Original Articles

Molecular Cloning and Characterization of cel2 from the Fungus Cochlibolus carbonum

, , &
Pages 1406-1411 | Received 27 Nov 2000, Accepted 30 Jan 2001, Published online: 22 May 2014

  • 1) Apel P.C., Panaccione D.G., Holden F.R., and Walton J.D., Cloning and targeted gene disruption of XYL1, a beta 1,4-xylanase gene from the maize pathogen Cochliobolus carbonum. Mol. Plant Microbe Interact., 6, 467-473 (1993).
  • 2) Apel-Birkhold P.C., and Walton J.D., Cloning, disruption, and expression of two endo-beta 1,4-xylanase genes, XYL2 and XYL3, from Cochliobolus carbonum. Appl. Environ. Microbiol., 62, 4129-4135 (1996).
  • 3) Schaeffer H.J., Leykam J., and Walton J.D., Cloning and targeted gene disruption of EXG1, encoding exo-beta 1,3-glucanase, in the phytopathogenic fungus Cochliobolus carbonum. Appl. Environ. Microbiol., 60, 594-598 (1994).
  • 4) Murphy J.M., and Walton J.D., Three extracellular proteases from Cochliobolus carbonum: cloning and targeted disruption of ALP1. Mol. Plant Microbe Interact., 9, 290-297 (1996).
  • 5) Görlach, J.M., Van der Knaap, E., and Walton, J.D., Cloning and targeted disruption of MLG1, a gene encoding two of three extracellular mixed-linked glucanases of Cochliobolus carbonum. Appl. Env. Microbiol., 64, 385-391 (1996).
  • 6) Scott-Craig J.S., Cheng Y.Q., Cervone F., De Lorenzo G., Pitkin J.W., and Walton J.D., Targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases. Appl. Environ. Microbiol. 64, 1497-1503 (1998).
  • 7) Wegener S., Ransom R.F., and Walton J.D., A unique eukaryotic beta-xylosidase gene from the phytopathogenic fungus Cochliobolus carbonum. Microbiology, 145, 1089-95 (1999).
  • 8) Annis, S.L., and Goodwin P.H., Recent advances in the molecular genetics of plant cell wall-degrading enzymes produced by plant pathogenic fungi. Eur. J. Plant Pathol., 103, 1-14 (1997).
  • 9) Walton, J.D., Deconstructing the cell wall. Plant Physiol., 104, 1113-1118 (1994).
  • 10) Kato, Y., and Nevin, D.J., Enzymatic dissociation of Zea shoot cell wall polysaccharides. Plant Physiol., 75, 740-744 (1984).
  • 11) Henrissat, B., and Barioch A., Updating the sequence-base classification of glycosyl hydrolases. Biochem. J., 316, 695-696 (1996).
  • 12) Sposato P., Ahn J.-H., and Walton J.D., Characterization and disruption of a gene in the maize pathogen Cochliobolus carbonum encoding a cellulase lacking a cellulose binding domain and hinge region. Mol. Plant- Microbe Interact., 8, 602-609 (1995).
  • 13) Pitkin, J.W., Panaccione, D.G., and Walton, J.D., A putative cyclic peptide efflux pump encoded by the TOXA gene of the plant-pathogenic fungus Cochliobolus carbonum. Micobiology, 142, 1557-1565 (1996).
  • 14) Ahn, J.-H., and Walton, J.D., Chromosomal organization of TOX2, the complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell, 8, 887-897 (1996).
  • 15) Tonukari, N.J., Scott-Craig, J.S., and Walton, J.D., The Cochliobolus carbonum SNF1 gene is required for cell-wall degrading enzyme expression and virulence in maize. Plant Cell, 12, 237-347 (2000).
  • 16) Hynes, M.J., Corrick, C.M., and King, J.A., Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Mol. Cell. Biol., 3, 1430-1439 (1983).
  • 17) Scott-Craig, J.S., Panaccione, D.G., Cervone, F., and Walton, J.D., Endopolygalacturonase is not required for pathogenicity of Cochliobolus carbonum on maize. Plant Cell, 2, 1191-1200 (1990).
  • 18) Panaccione, D.G., Scott-Craig, J.S., Pocard, J.-A., and Walton, J.D., A cyclic peptide synthetase gene required for pathogenicity of the fungus Cochliobolus carbonum on maize. Proc. Natl. Acad. Sci., 89, 6590-6594 (1992).
  • 19) Orbach, M.J., Porro, E.B., and Yanofsky, C., Cloning and characterization of the gene for betatubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable maker. Mol. Cell. Biol., 6, 2452-2461 (1986).
  • 20) Nielson H., Engelbrecht J., Brunak S., and von Heijne. G., Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Prot. Eng. 10, 1-6 (1997).
  • 21) Knowles, J., Lehtovaara, P., and Teeri, T.T., Cellulase families and their genes. Trends Biotechnol., 5, 225-261 (1987).
  • 22) Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res., 25, 3389-3402 (1997).
  • 23) Kleywegt, G.J., Zou J.-Y., Divne C., Davies G.J., Sinning, I., Stahlberg, J., Reinikainen, T. Srisodsuk, M., Teeri, T.T., and Jones, T.A., The crystal sturcture of the catalytic core domain of endoglucanase I from Trichoderma reesei at 3.6Å resolution and a comparison with related enzymes. J. Mol. Biol., 272, 383-397 (1997).
  • 24) Davies, G.J., Ducros V., Lewis, R.J. Borchert, T.V., and Schulein M., Oligosaccharide specificity of a family 7 endoglucanase: insertion of potential sugarbinding subsites. J. Biotech., 57, 91-100 (1997).
  • 25) Kitamoto, N., Go, M., Shibayama, T., Kimura, T., Kito, Y., Ohmiya, K., and Tsukagoshi, N., Molecular cloning, purification and characterization of two endo-1,4-beta-glucanases from Aspergillus oryzae KBN616. Appl. Microbiol. Biotechnol., 46, 538-544 (1996).
  • 26) Penttila, M., Lehtovaara, P., Nevalainen, H., Bhikhabhai, R., and Knowles, J., Homology between cellulase gnes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene. Gene, 45, 253-263 (1986).
  • 27) Sheppard, P.O., Grant, F.J., Oort, P.J., Sprecher, C.A., Forster, D.C., Hagen, F.S., Upshall, A., Mckight, G.L., and O’Hara, P.J., The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNA from Fusarium oxysporum. Gene, 150, 163-167 (1994).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.