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Original Articles

Equilibrium Dialysis Measurements of the Ca2+-Binding Properties of Recombinant Radish Vacuolar Ca2+-Binding Protein Expressed in Escherichia coli

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Pages 2382-2387 | Received 21 May 2002, Accepted 11 Jul 2002, Published online: 22 May 2014

  • 1) Bush, D. S., Calcium regulation in plant cells and its role in signaling. Annu. Rev. Plant Physiol. Plant Mol. Biol., 46, 95-122 (1995).
  • 2) Sanders, D., Brownlee, C., and Harper, J. F., Communicating with calcium. Plant Cell, 11, 691-706 (1999).
  • 3) Sze, H., Liang, F., and Hwang, I., Diversity and regulation of plant Ca2+ pumps: insight from expression in yeast. Annu. Rev. Plant Physiol. Plant Mol. Biol., 51, 433-462 (2000).
  • 4) Mogami, H., Gardner, J., Gerasimenko, O. V., Camello, P., Petersen, O. H., and Tepikin, A.V., Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells. J. Physiol., 518, 463-467 (1999).
  • 5) Ueoka-Nakanishi, H., Nakanishi, Y., Tanaka, Y., and Maeshima, M., Properties and molecular cloning of Ca2+/H+ antiporter in the vacuolar membrane of mung bean. Eur. J. Biochem., 262, 417-425 (1999).
  • 6) Yuasa, K., and Maeshima, M., Purification, properties, and molecular cloning of a novel Ca2+-binding protein in radish vacuoles. Plant Physiol., 124, 1069-1078 (2000).
  • 7) Clark, G. B., and Roux, S. J., Annexins of plant cells. Plant Physiol., 109, 1133-1139 (1995).
  • 8) Snedden, W. A., and Fromm, H., Calmodulin, calmodulin-related proteins and plant responses to the environment. Trends Plant Sci., 3, 299-304 (1998).
  • 9) Crofts, A. J., and Denecke, J., Calreticulin and calnexin in plants. Trends Plant Sci., 3, 396-399 (1998).
  • 10) Li, Z., and Komatsu, S., Molecular cloning and characterization of calreticulin, a calcium protein involved in the regeneration of rice cultured suspension cells. Eur. J. Biochem., 267, 737-745 (2000).
  • 11) Marty, F., Plant vacuoles. Plant Cell, 11, 587-599 (1999).
  • 12) Yuasa, K., and Maeshima, M., Organ specificity of a vacuolar Ca2+-binding protein RVCaB in radish and its expression under Ca2+-deficient conditions. Plant Mol. Biol., 47, 633-640 (2001).
  • 13) Maruyama, K., Mikawa, T., and Ebashi, S., Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. J. Biochem., 95, 165-174 (1984).
  • 14) Campbell, K. P., MacLennan, D. H., and Jorgensen, A. O., Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye “Stains-all”. J. Biol. Chem., 258, 11267-11273 (1983).
  • 15) Baksh, S., and Michalak, M., Expression of calreticulin in Escherichia coli and identification of its Ca2+ binding domains. J. Biol. Chem., 266, 21458-21465 (1991).
  • 16) Colowick, S. P., and Womack, F. C., Binding of diffusible molecules by macromolecules: rapid measurement by rate of dialysis. J. Biol. Chem., 244, 774-777 (1969).
  • 17) Kawasaki, H., Kasai, H., and Okuyama, T., Protein analyses and reagents: microscale assay of calcium-binding activity of proteins and peptides using a nitrocellulose membrane. Anal. Biochem., 148, 297-302 (1985).
  • 18) Drabikowski, W., and Brzeska, R. S., Tryptic fragments of calmodulin: Ca2+- and Mg2+-induced conformational changes. J. Biol. Chem., 257, 11584-11590 (1982).
  • 19) Ogawa, Y., and Tanokura, M., Calcium binding to calmodulin: effects of ionic strength, Mg2+, pH and temperature. J. Biochem., 95, 19-28 (1984).
  • 20) Evans, T. C., and Nelsestuen, G. L., Calcium and membrane-binding properties of monomeric and multimeric annexin II. Biochemistry, 33, 13231-13238 (1994).
  • 21) Maeshima, M., Tonoplast transporters: organization and function. Annu. Rev. Plant Physiol. Plant Mol. Biol., 52, 469-497 (2001).

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