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Original Articles

Purification and Characterization of β-1,6-Glucanase of Streptomyces rochei Application in the Study of Yeast Cell Wall Proteins

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Pages 2515-2519 | Received 17 Jun 2002, Accepted 15 Jul 2002, Published online: 22 May 2014

  • 1) Cabib, E., Roberts, R., and Browers, B., Synthesis of the yeast cell wall and its regulation. Annu. Rev. Biochem., 51, 763-793 (1982).
  • 2) Cid, V. J., Duran, A., Rey, F., Snyder, M. P., Nombela, C., and Sanchez, M., Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol. Rev., 59, 345-386 (1995).
  • 3) Kollar, R., Reinhold, B. B., Petrakova, E., Yen, H. J. C., Ashwell, G., Drgonova, J., and Klis, F. M., Cell wall assembly in yeast. Yeast, 10, 851-869 (1994).
  • 4) Kapteyn, J. C., Montijn, R. C., Vink, E., Cruz, J., Llobell, A., Douwes, J. E., Shimoi, H., Lipke, P. N., and Klis, F. M., Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked β-1,3-/ β-1,6-glucan heteropolymer. Glycobiology, 6, 337-345 (1996).
  • 5) Kapteyn, J. C., Klis, F. M., and Cabib, E., Architecture of the yeast cell wall: β-1,6- glucan interconnects mannoproteins, β-1,3-glucan, and chitin. J. Biol. Chem., 272, 17762-17775 (1997).
  • 6) Rombouts, F. M., and Phaff, H. J., Lysis of yeast cell walls. Lytic beta-1,6-glucanase from Bacillus circulans WL-12. Eur. J. Biochem., 63, 109-120 (1976).
  • 7) Molano, J., Bowers, B., and Cabib, E., Distribution of chitin in the yeast cell wall. An ultrastructural and chemical study. J. Cell Biol., 85, 199-212 (1980).
  • 8) Cruz, J. D. L., Pintor-Toro, J. A., Benitez, T., and Llobell, A., Purification and characterization of an endo-β-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism. J. Bacteriol., 177, 1864-1871 (1995).
  • 9) Cruz, J. D. L., and Llobell, A., Purification and properties of a basic endo-β-1,6-glucanase (BGN16.1) from the antagonistic fungus Trichoderma harzianum. Eur. J. Biochem., 265, 145-151 (1999).
  • 10) Yoshida, M., Nishi, A., Ohbuchi, K., Hojo, T., Matsuzawa, A., Hamachi, M., and Kumagai, C., Screening of the lytic enzyme of the red yeast Phaffia rhodozyma cell wall and extraction of astaxanthin. Seibutsu kougaku (in Japanese), 75, 229-238 (1997).
  • 11) Nishi, A., Ohbuchi, K., Hamachi, M., and Kumagai, C., Breeding of a constitutive-producing strain of Streptomyces rochei PHA-34 that produces the lytic enzyme for Phaffia rhodozyma and extraction of astaxanthin using the enzyme. Seibutsu kougaku (in Japanese), 77, 60-65 (1999).
  • 12) Ohbuchi, K., Niski, A., Hamachi, M., and Kumagai, C., Protoplast formation in yeast by the lytic enzyme of Streptomyces rochei DB-34 and their regeneration. Seibutsu kougaku (in Japanese), 77, 137-146 (1999).
  • 13) Yu, S., Olsen, C. E., and Marcussen, J., Methods for the assay of 1,5-anhydro-D-fructose and α-1,4-glucan lyase. Carbohydrate Research, 305, 73-82 (1998).
  • 14) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
  • 15) Sikorski, R. S., and Hieter, P., A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27 (1989).
  • 16) Shimoi, H., Iimura, Y., and Obata, T., Molecular cloning of CWP1: A gene encoding a Saccharomyces cerevisiae cell wall protein solubilized with Rarobacter faecitabidus Protease I. J. Biochem., 118, 302-311 (1995).

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