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Bioscience, Biotechnology, and Biochemistry Volume 67, 2003 - Issue 8
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Original Articles

Peptide Mapping and Assessment of Cryoprotective Activity of 26/27-kDa Dehydrin from Soybean Seeds

Michiko MOMMANational Food Research Institute2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
,
Shigenobu KANEKONational Food Research Institute2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan;Current address: National Institute of Crop Science2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan
,
Kazutomo HARAGUCHINational Food Research Institute2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
&
Ushio MATSUKURANational Food Research Institute2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan;Current address: National Institute of Crop Science2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan
Pages 1832-1835 | Received 24 Feb 2003, Accepted 07 May 2003, Published online: 22 May 2014

  • 1) Close, T. J., Dehydrins: A commonalty in the response of plants to dehydration and low temperature. Physiologia Plantarum, 100, 291-296 (1997).
  • 2) Momma, M., Haraguchi, K., Saito, M., Chikuni, K., and Harada, K., Purification and characterization of the acid soluble 26-kDa polypeptide from soybean seeds. Biosci. Biotechnol. Biochem., 61, 1286-1291 (1997).
  • 3) Momma, M., Kaneko, S., and Matsukura, U., Characterization of soybean dehydrin (group2 LEA protein): Localization and accumulation in seed, cryoprotective activity on lactate dehydrogenase, and molecular weight diversity among Japanese soy varieties. Rep. Natl. Food Res. Inst. (in Japanese), 66, 21-28 (2002).
  • 4) Lin, C., and Thomashow, M. F., A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotective activity. Biochem. Biophys. Res. Commun., 183, 1103-1108 (1992).
  • 5) Yamauchi, F., Sato, K., and Yamagishi, T., Isolation and partial characterization of salt-extractable globulin from soybean seeds. Agric. Biol. Chem., 48, 645-650 (1984).
  • 6) Honjoh, K., Matsumoto, H., Shimizu, H., Ooyama, K., Tanaka, K., Oda, Y., Tanaka, R., Joh, T., Suga, K., Miyamoto, T., Iio, M., and Hatano, S., Cryoprotective activities of group 3 late embryogenesis abundant protein from Chlorella vulgaris C-27. Biosci. Biotechnol. Biochem., 64, 1656-1663 (2000).

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