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Bioscience, Biotechnology, and Biochemistry Volume 69, 2005 - Issue 5
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Original Articles

A Variant Protein from φ29 Replication Gene, Gene 1, Did Not Form Homo-Polymer Due to a Single Amino Acid Substitution Near the Carboxyl Terminus

Kazuya HASHIYAMADepartment of Genetics, Life Science Institute, Sophia University
,
Ari TAKEUCHIDepartment of Genetics, Life Science Institute, Sophia University
&
Osamu MAKINODepartment of Genetics, Life Science Institute, Sophia University
Pages 1045-1048 | Received 14 Jan 2005, Accepted 15 Mar 2005, Published online: 22 May 2014

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  • 2) Bravo, A., and Salas, M., Initiation of bacteriophage φ29 DNA replication in vivo: Assembly of a membrane-associated multiprotein complex. J. Mol. Biol., 269, 102–112 (1997).
  • 3) Bravo, A., Illana, B., and Salas, M., Compartmentalization of phage φ29 DNA replication: interaction between the primer terminal protein and the membrane-associated protein p1. EMBO J., 19, 5575–5584 (2000).
  • 4) Takeuchi, A., Makino, O., Matsumoto, K., and Hirokawa, H., Inhibition of protein synthesis from DNA replication genes by the product of gene1, a dna gene of Bacillus subtilis phage φ29. J. Gen. Appl. Microbiol., 41, 507–516 (1995).
  • 5) Takeuchi, A., Makino, O., and Hirokawa, H., Gene1, one of the dna genes of bacteriophage φ29 represses other dna genes through binding to mRNA. Biochem. Biophys. Res. Commun., 243, 566–571 (1998).
  • 6) Bravo, A., and Salas, M., Polymerization of bacteriophage φ29 replication protein p1 into protofilament sheets. EMBO J., 17, 6096–6105 (1998).
  • 7) Serrano-Heras, G., Salas, M., and Bravo, A., In vivo assembly of phage φ29 replication protein p1 into membrane-associated multimeric structures. J. Biol. Chem., 278, 40771–40777 (2003).
  • 8) Kyte, J., and Doolittle, R. F., A simple method for displaying the hydropathic character of a protein. J. Mol. Biol., 157, 105–142 (1982).
  • 9) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685 (1970).
  • 10) Bravo, A., Serrano-Heras, G., and Salas, M., A single amino acid substitution within a coiled-coil motif changes the assembly of a 53-amino acid protein from two-dimensional sheets to filamentous structures. J. Biol. Chem., 276, 21250–21256 (2001).
  • 11) Hashiyama, K., Takeuchi, A., and Makino, O., Effects of single amino acid substitutions at the predicted coiled-coil or hydrophobic region on the self-assembly of φ29 replication protein, gp1. Biochem. Biophys. Res. Commun., 331, 1310–1316 (2005).
  • 12) Shagger, H., and Jagow, G., Tricine-sodium dodecyl sulfate–polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem., 166, 368–379 (1987).

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