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Bioscience, Biotechnology, and Biochemistry Volume 72, 2008 - Issue 10
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Original Articles

Isolation and Characterization of a Novel Equol-Producing Bacterium from Human Feces

Shin-ichiro YOKOYAMADepartment of Applied Microbiology, Gifu Prefectural Research Institute for Bioengineering
&
Tohru SUZUKIThe United Graduate School of Agricultural Science, Gifu University
Pages 2660-2666 | Received 12 May 2008, Accepted 27 Jun 2008, Published online: 22 May 2014

  • 1) Brienholt, V., and Larsen, J. C., Detection of weak estrogenic flavonoids using a recombinant yeast strain and a modified MCF7 cell proliferation assay. Chem. Res. Toxicol., 11, 622–629 (1998).
  • 2) Kuiper, G. G., Lemmen, J. G., Carlsson, B., Corton, J. C., Safe, S. H., van der Saag, P. T., van der Burg, B., and Gustafsson, J. A., Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinol., 139, 4252–4263 (1998).
  • 3) Setchell, K. D. R., Brown, N. M., and Lydeking-Olsen, E., The clinical importance of the metabolite equol—A clue to the effectiveness of soy and isoflavones. J. Nutr., 132, 3577–3584 (2002).
  • 4) Duncan, A. M., Merz-Demlow, B. E., Xu, X., Phipps, W. R., and Kurzer, M. S., Premenopausal equol excretors show plasma hormone profiles associated with lowered risk of breast cancer. Cancer Epidemiol. Biomarkers Prev., 9, 581–586 (2000).
  • 5) Ingram, D., Sanders, K., Kolybaba, M., and Lopez, D., Case-control study of phyto-oestrogens and breast cancer. Lancet, 350, 990–994 (1997).
  • 6) Akaza, H., Miyanaga, N., Takashima, N., Naito, S., Hirano, Y., Tsukamoto, T., and Mori, M., Is daidzein non-metabolizer a high risk for prostate cancer? A case-controlled study of serum soybean isoflavone concentration. Jpn. J. Clin. Oncol., 32, 296–300 (2002).
  • 7) Ozasa, K., Nakao, M., Watanabe, Y., Hayashi, K., Miki, T., Mikami, K., Mori, M., Sakauchi, F., Washio, M., Ito, Y., Suzuki, K., Wakai, K., Tamakoshi, A., and JACC Study Group, Serum phytoestrogens and prostate cancer risk in a nested case-control study among Japanese men. Cancer Sci., 95, 65–71 (2004).
  • 8) Lydeking-Olsen, E., Jensen, J. B. E., Setchell, K. D. R., Damhus, M., and Jensen, T. H., Isoflavone-rich soymilk prevents bone-loss in lumbar spine of postmenopausal women. J. Nutr., 132, 581S (2002).
  • 9) Ueno, T., Uchiyama, S., and Kikuchi, N., The role of intestinal bacteria on biological effects of soy isoflavones in humans. J. Nutr., 132, 594S (2002).
  • 10) Kelly, G. E., Joannou, G. E., Reeder, A. Y., Nelson, C., and Waring, M. A., The variable metabolic response to dietary isoflavones in humans. Proc. Soc. Exp. Biol. Med., 208, 40–43 (1995).
  • 11) Kirkman, L. M., Lampe, J. W., Campbell, D. R., Martini, M. C., and Slavin, J. L., Urinary lignan and isoflavonoid excretion in men and women consuming vegetable and soy diets. Nutr. Cancer, 24, 1–12 (1995).
  • 12) Lampe, J. W., Karr, S. C., Hutchins, A. M., and Slavin, J. L., Urinary equol excretion with a soy challenge: influence of habitual diet. Proc. Soc. Exp. Biol. Med., 217, 335–339 (1998).
  • 13) Morton, M. S., Arisaka, O., Miyake, N., Morgan, L. D., and Evans, B. A. J., Phytoestrogen concentrations in serum from Japanese men and women over forty years of age. J. Nutr., 132, 3168–3171 (2002).
  • 14) Raffi, F., Davis, C., Park, M., Heinze, T. M., and Beger, R. D., Variations in metabolism of the soy isoflavonoid daidzein by human intestinal microfloras from different individuals. Arch. Microbiol., 180, 11–16 (2003).
  • 15) Decroos, K., Vanhemmens, S., Cattoir, S., Boon, N., and Verstraete, W., Isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions. Arch. Microbiol., 183, 45–55 (2005).
  • 16) Hur, H. G., Lay, J. O., Jr., Beger, R. D., Freeman, J. P., and Rafii, F., Isolation of human intestinal bacteria metabolizing the natural isoflavone glycosides daidzin and genistin. Arch. Microbiol., 174, 422–428 (2000).
  • 17) Wang, X. L., Shin, K. H., Hur, H. G., and Kim, S. I., Enhanced biosynthesis of dihydrodaidzein and dihydrogenistein by a newly isolated bovine rumen anaerobic bacterium. J. Biotechnol., 115, 261–269 (2005).
  • 18) Tamura, M., Tsushida, T., and Shinohara, K., Isolation of an isoflavone-metabolizing, Clostridium-like bacterium, strain TM-40, from human faeces. Anaerobe, 13, 32–35 (2007).
  • 19) Wang, X. L., Hur, H. G., Lee, J. H., Kim, K. T., and Kim, S. I., Enantioselective synthesis of S-equol from dihydrodaizein by a newly isolated anaerobic human intestinal bacterium. Appl. Environ. Microbiol., 71, 214–219 (2005).
  • 20) Minamida, K., Tanaka, M., Abe, A., Sone, T., Tomita, F., Hara, H., and Asano, K., Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine. J. Biosci. Bioeng., 102, 247–250 (2006).
  • 21) Maruo, T., Sakamoto, M., Ito, C., Toda, T., and Benno, Y., Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella. Int. J. Syst. Evol. Microbiol., 58, 1221–1227 (2008).
  • 22) Yokoyama, S., and Kuzuguchi, T., Rapid and convenient detection of urinary equol by thin-layer chromatography. J. Nutr. Sci. Vitaminol., 53, 43–47 (2007).
  • 23) Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J. H., Zhang, Z., Miller, W., and Lipman, D. J., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25, 3389–3402 (1997).
  • 24) Thompson, J. D., Higgins, D. G., and Gibson, T. J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22, 4673–4680 (1994).
  • 25) Lau, S. K. P., Woo, P. C. Y., Woo, G. K. S., Fung, A. M. Y., Wong, M. K. M., Chan, K. M., Tam, D. M. W., and Yuen, K. Y., Eggerthella hongkongensis sp. nov. and Eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia. Diagn. Microbiol. Infect. Dis., 49, 255–263 (2004).
  • 26) Morito, K., Hirose, T., Kinjo, J., Hirakawa, T., Okawa, M., Nohara, T., Ogawa, S., Inoue, S., Muramatsu, M., and Masamune, Y., Interaction of phytoestrogens with estrogen receptors α and β. Biol. Pharm. Bull., 24, 351–356 (2001).
  • 27) Kostelac, D., Rechkemmer, G., and Briviba, K., Phytoestrogens modulate binding response of estrogen receptors α and β to the estrogen response element. J. Agric. Food Chem., 51, 7632–7635 (2003).
  • 28) Muthyala, R. S., Ju, Y. H., Sheng, S., Williams, L. D., and Doerge, D. R., Equol, a natural estrogenic metabolite from soy isoflavones: convenient preparation and resolution of R- and S-equols and their differing binding and biological activity through estrogen receptors alpha and beta. Bioorg. Med. Chem., 12, 1559–1567 (2004).
  • 29) Setchell, K. D. R., Clerici, C., Lephart, E. D., Cole, S. J., Heenan, C., Castellani, D., Wolfe, B. E., Nechemias-Zimmer, L., Brown, N. M., Lund, T. D., Handa, R. J., and Heubi, J. E., S-Equol, a potent ligand for estrogen receptor β, is the exclusive enantiomeric form of the soy isoflavone metabolite produced by human intestinal bacterial flora. Am. J. Clin. Nutr., 81, 1072–1079 (2005).
  • 30) Joannou, G. E., Kelly, G. E., Reeder, A. Y., Waring, M., and Nelson, C., A urinary profile study of dietary phytoestrogens. The identification and mode of metabolism of new isoflavonoids. J. Steroid Biochem. Mol. Biol., 54, 167–184 (1995).
  • 31) Atkinson, C., Berman, S., Humbert, O., and Lampe, J. W., In vitro incubation of human feces with daidzein and antibiotics suggests interindividual differences in the bacteria responsible for equol production. J. Nutr., 134, 596–599 (2004).
  • 32) Blair, R. M., Appt, S. E., Franke, A. A., and Clarkson, T. B., Treatment with antibiotics reduces plasma equol concentration in cynomolgus monkeys (Macaca fascicularis). J. Nutr., 133, 2262–2267 (2003).

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