Advanced search
Publication Cover
Bioscience, Biotechnology, and Biochemistry Volume 72, 2008 - Issue 12
Journal homepage
109
Views
5
CrossRef citations to date
0
Altmetric
Original Articles

Recombinant Protein Secretion in Pseudozyma flocculosa and Pseudozyma antarctica with a Novel Signal Peptide

Yali CHENGDepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval
,
Tyler J. AVISDepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval;Department of Chemistry, Carleton University
,
Sébastien BOLDUCDepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval
,
Yingyi ZHAODepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval
,
Raphaël ANGUENOTDepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval
,
Bertrand NEVEUDepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval
,
Caroline LABBÉDepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval
,
François BELZILEDepartment of Phytology, C.-E. Marchand, University Laval
&
Richard R. BÉLANGERDepartment of Phytology, Horticulture Research Centre, Envirotron, University Laval
 show all
Pages 3158-3166 | Received 19 May 2008, Accepted 28 Aug 2008, Published online: 22 May 2014

  • 1) Avis, T. J., Cheng, Y. L., Zhao, Y. Y., Bolduc, S., Neveu, B., Anguenot, R., Labbé, C., Belzile, F., and Bélanger, R. R., The potential of Pseudozyma yeastlike epiphytes for the production of heterologous recombinant proteins. Appl. Microbiol. Biotechnol., 69, 304–311 (2005).
  • 2) Neveu, B., Michaud, M., Belzile, F., and Bélanger, R. R., The Pseudozyma flocculosa actin promoter allows the strong expression of a recombinant protein in the Pseudozyma species. Appl. Microbiol. Biotechnol., 74, 1300–1307 (2007).
  • 3) Neveu, B., Belzile, F., and Bélanger, R. R., Cloning of the glyceraldehyde-3-phosphate dehydrogenase gene from Pseudozyma flocculosa and functionality of its promoter in two Pseudozyma species. Antonie Van Leeuwenhoek, 92, 245–255 (2007).
  • 4) Avis, T. J., Anguenot, R., Neveu, B., Bolduc, S., Zhao, Y. Y., Cheng, Y. L., Labbé, C., Belzile, F., and Bélanger, R. R., Usefulness of heterologous promoters in the Pseudozyma flocculosa gene expression system. Biosci. Biotechnol. Biochem., 72, 456–462 (2008).
  • 5) Crabbe, T., Weir, A. N., Walton, E. F., Brown, M. E., Sutton, C. W., Tretout, N., Bonnerjea, J., Lowe, P. A., and Yarranton, G. T., The secretion of active recombinant human gastric lipase by Saccharomyces cerevisiae. Protein Expr. Purif., 7, 229–236 (1996).
  • 6) Radzio, R., and Kück, U., Efficient synthesis of the blood-coagulation inhibitor hirudin in the filamentous fungus Acremonium chrysogenum. Appl. Microbiol. Biotechnol., 48, 58–65 (1997).
  • 7) Morel, N., and Massoulié, J., Expression and processing of vertebrate acetylcholinesterase in the yeast Pichia pastoris. Biochem. J., 328, 121–129 (1997).
  • 8) van den Hondel, C. A. M. J. J., Punt, P. J., and van Gorcom, R. F. M., Heterologous gene expression in filamentous fungi. In “More Gene Manipulations in Fungi,” eds. Bennett, J. W., and Lasure, L. L., Academic Press, San Diego, pp. 396–428 (1991).
  • 9) Record, E., Punt, P. J., Chamkha, M., Labat, M., van den Hondel, C. A. M. J. J., and Asther, M., Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme. Eur. J. Biochem., 269, 602–609 (2002).
  • 10) Golden, A., Austen, D. A., van Schravendijk, M. R., Sullivan, B. J., Kawasaki, E. S., and Osburne, M. S., Effect of promoters and signal sequences on the production of secreted HIV-1 gp120 protein in the baculovirus system. Protein Expr. Purif., 14, 8–12 (1998).
  • 11) Shevchenko, A., Wilm, M., Vorm, O., and Mann, M., Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem., 68, 850–858 (1996).
  • 12) Cheng, Y. L., Belzile, F., Tanguay, P., Bernier, L., and Bélanger, R. R., Establishment of a gene transfer system for Pseudozyma flocculosa, an antagonistic fungus of powdery mildew fungi. Mol. Genet. Genomics, 266, 96–102 (2001).
  • 13) Cheng, Y. L., and Bélanger, R. R., Protoplast preparation and regeneration from spores of the biocontrol fungus Pseudozyma flocculosa. FEMS Microbiol. Lett., 190, 287–291 (2000).
  • 14) Draber, P., Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver. Electrophoresis, 12, 453–456 (1991).
  • 15) Nielsen, H., Engelbrecht, I., Brunak, S., and Von Heijne, G., Identification of eukaryotic and prokaryotic signal peptides and prediction of their cleavage sites. Protein Eng., 10, 1–6 (1997).
  • 16) Bokman, S. H., and Ward, W. W., Renaturation of Aequoraea green fluorescent protein. Biochem. Biophys. Res. Commun., 101, 1372–1380 (1981).
  • 17) Haguenauer-Tsapis, R., and Hinnen, A., A deletion that includes the signal peptidase cleavage site impairs processing, glycosylation, and secretion of cell surface yeast acid phosphatase. Mol. Cell. Biol., 4, 2668–2675 (1984).
  • 18) Schauer, I., Scott, E., Gross, C., and Schekman, R., Invertase signal and mature sequence substitutions that delay intercompartmental transport of active enzyme. J. Cell Biol., 100, 1664–1675 (2005).
  • 19) Ward, M., Wilson, L. J., Kodama, K. H., Rey, M. W., and Berka, R. M., Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion. Bio/Technology, 8, 435–440 (1990).
  • 20) van Gemeren, I. A., Beijersbergen, A., Muster, W., Gouka, R. J., van den Hondel, C. A. M. J. J., and Verrips, C. T., The effect of pre- and pro-sequences and multicopy integration on heterologous expression of the Fusarium solani pisi cutinase gene in Aspergillus awamori. Appl. Microbiol. Biotechnol., 45, 755–763 (1996).
  • 21) Braspenning, J., Meschede, W., Marchini, A., Muller, M., Gissmann, L., and Tommasino, M., Secretion of heterologous proteins from Schizosaccharomyces pombe using the homologous leader sequence of pho1+ acid phosphatase. Biochem. Biophys. Res. Commun., 245, 166–171 (1998).
  • 22) Eiden-Plach, A., Zagorc, T., Heintel, T., Carius, Y., Breinig, F., and Schmitt, M. J., Viral preprotoxin signal sequence allows efficient secretion of green fluorescent protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. Appl. Environ. Microbiol., 70, 961–966 (2004).
  • 23) Raemaekers, R. J., de Muro, L., Gatehouse, J. A., and Fordham-Skelton, A. P., Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide. Eur. J. Biochem., 265, 394–403 (1999).
  • 24) Kunze, I., Hensel, G., Adler, K., Bernard, J., Neubonh, B., Nilsson, C., Stoltenburg, R., Kohlwein, S. D., and Kunze, G., The green fluorescent protein targets secretory proteins to the yeast vacuole. Biochim. Biophys. Acta, 1410, 287–298 (1999).
  • 25) Li, J., Xu, H., Bentley, W. E., and Rao, G., Impediments to secretion of green fluorescent protein and its fusion from Saccharomyces cerevisiae. Biotechnol. Prog., 18, 831–838 (2002).
  • 26) Gordon, C. L., Archer, D. B., Jeenes, D. J., Doonan, J. H., Wells, B., Trinci, A. P. J., and Robson, G. D., A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger. J. Microbiol. Methods, 42, 39–48 (2000).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.