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Expert Opinion on Drug Discovery Volume 4, 2009 - Issue 6
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An overview of cell phenotypes in HCS: limitations and advantages

Fabio Gasparri Department of Cell Biology, Oncology Business Unit, Nerviano Medical Sciences S.r.l., Viale Pasteur 10, 20014, Nerviano (Milano), Italy +39 0331 581706; +39 0331 581233; [email protected]
Pages 643-657 | Published online: 21 May 2009

Bibliography

  • Giuliano KA, DeBiasio RL, Dunlay RT, et al. High content screening: a new approach to easing key bottlenecks in the drug discovery process. J Biomol Screen 1997;2:249-59
  • Taylor DL. Past, present, and future of high content screening and the field of cellomics. Methods Mol Biol 2007;356:3-18
  • Edwards BS, Oprea T, Prossnitz ER, Sklar LA. Flow cytometry for high-throughput, high-content screening. Curr Opin Chem Biol 2004;8(4):392-8
  • Krutzik PO, Crane JM, Clutter MR, Nolan GP. High-content single-cell drug screening with phosphospecific flow cytometry. Nat Chem Biol 2008;4(2):132-42
  • Minor LK. Label-free cell-based functional assays. Comb Chem High Throughput Screen 2008;11(7):573-80
  • Xi B, Yu N, Wang X, et al. The application of cell-based label-free technology in drug discovery. Biotechnol J 2008;3(4):484-95
  • Lopez MF, Mikulskis A, Golenko E, et al. High-content proteomics: fluorescence multiplexing using an integrated, high-sensitivity, multiwavelength charge-coupled device imaging system. Proteomics 2003;3(7):1109-16
  • Gough AH, Johnston PA. Requirements, features, and performance of high content screening platforms. Methods Mol Biol 2007;356:41-61
  • Dunlay RT, Czekalski WJ, Collins MA. Overview of informatics for high content screening. Methods Mol Biol 2007;356:269-80
  • Giuliano KA, Taylor DL. Fluorescent-protein biosensors: new tools for drug discovery. Trends Biotechnol 1998;16(3):135-40
  • Giuliano KA, Taylor DL, Waggoner AS. Reagents to measure and manipulate cell functions. Methods Mol Biol 2007;356:141-63
  • Rausch O. High content cellular screening. Curr Opin Chem Biol 2006;10(4):316-20
  • Krausz E. High-content siRNA screening. Mol Biosyst 2007;3(4):232-40
  • Gasparri F, Sola F, Bandiera T, et al. High-content analysis of kinase activity in cells. Comb Chem High Throughput Screen 2008;11(7):523-36
  • Dragunow M. High-content analysis in neuroscience. Nat Rev Neurosci 2008;9(10):779-88
  • O'Brien P, Haskins JR. In vitro cytotoxicity assessment. Methods Mol Biol 2007;356:415-25
  • Ding GJF, Fischer PA, Boltz RC, et al. Characterization and quantitation of NF-κB nuclear translocation induced by Interleukin-1 and Tumor Necrosis Factor-α. J Biol Chem 1998;273(44):28897-905
  • Minguez JM, Giuliano KA, Balachandran R, et al. Synthesis and high content cell-based profiling of simplified analogues of the microtubule stabilizer ( + )-discodermolide. Mol Cancer Ther 2002;1(14):1305-13
  • Giuliano KA. High-content profiling of drug-drug interactions: cellular targets involved in the modulation of microtubule drug action by the antifungal ketoconazole. J Biomol Screen 2003;8(2):125-35
  • Liebel U, Starkuviene V, Erfle H, et al. A microscope-based screening platform for large-scale functional protein analysis in intact cells. FEBS Lett 2003;554:394-8
  • Vogt A, Cooley KA, Brisson M, et al. Cell-active dual specificity phosphatase inhibitors identified by high-content screening. Chem Biol 2003;10(8):733-42
  • Abraham VC, Taylor DL, Haskins JR. High content screening applied to large-scale cell biology. Trends Biotechnol 2004;22(1):15-22
  • Conrad C, Erfle H, Warnat P, et al. Automatic identification of subcellular phenotypes on human cell arrays. Genome Res 2004;14(6):1130-6
  • Ghosh RN, Grove L, Lapets O. A quantitative cell-based high-content screening assay for the epidermal growth factor receptor-specific activation of mitogen-activated protein kinase. Assay Drug Dev Technol 2004;2(5):473-81
  • Lövborg H, Nygren P, Larsson R. Multiparametric evaluation of apoptosis: effects of standard cytotoxic agents and the cyanoguanidine CHS 828. Mol Cancer Ther 2004;3(5):521-6
  • Perlman ZE, Slack MD, Feng Y, et al. Multidimensional drug profiling by automated microscopy. Science 2004;306:1194-8
  • Starkuviene V, Liebel U, Simpson JC, et al. High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic. Genome Res 2004;14(10A):1948-56
  • Bertelsen M, Sanfridson A. Inflammatory pathway analysis using a high content screening platform. Assay Drug Dev Technol 2005;3(3):261-71
  • Giuliano KA, Cheung WS, Curran DP, et al. Systems cell biology knowledge created from high content screening. Assay Drug Dev Technol 2005;3(5):501-13
  • Carpenter AE, Jones TR, Lamprecht MR, et al. CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol 2006;7(10):R100
  • Fryknäs M, Rickardson L, Wickström M, et al. Phenotype-based screening of mechanistically annotated compounds in combination with gene expression and pathway analysis identifies candidate drug targets in a human squamous carcinoma cell model. J Biomol Screen 2006;11(5):457-68
  • Neumann B, Held M, Liebel U, et al. High-throughput RNAi screening by time-lapse imaging of live human cells. Nat Methods 2006;3(5):385-90
  • Nickischer D, Laethem C, Trask OJ Jr, et al. Development and implementation of three mitogen-activated protein kinase (MAPK) signaling pathway imaging assays to provide MAPK module selectivity profiling of kinase inhibitors: MK2-EGFP translocation, c-Jun, and ERK activation. Methods Enzymol 2006;414:389-418
  • Trask OJ Jr, Baker A, Williams RG, et al. Assay development and case history of a 32K-biased library high-content MK2-EGFP translocation screen to identify p38 mitogen-activated protein kinase inhibitors on the ArrayScan 3.1 imaging platform. Methods Enzymol 2006;414:419-39
  • Vassal E, Barette C, Fonrose X, et al. Miniaturization and validation of a sensitive multiparametric cell-based assay for the concomitant detection of microtubule-destabilizing and microtubule-stabilizing agents. J Biomol Screen 2006;11(4):377-89
  • Williams RG, Kandasamy R, Nickischer D, et al. Generation and characterization of a stable MK2-EGFP cell line and subsequent development of a high-content imaging assay on the Cellomics ArrayScan platform to screen for p38 mitogen-activated protein kinase inhibitors. Methods Enzymol 2006;414:364-89
  • Kwon YJ, Genovesio A, Youl Kim N, et al. High-content classification of nucleocytoplasmic import or export inhibitors. J Biomol Screen 2007;12(5):621-7
  • Low J, Huang S, Dowless M, et al. High-content imaging analysis of knockdown effects of validated siRNAs and antisense oligonucleotides. J Biomol Screen 2007;12(6):775-88
  • Loo L-H, Wu LF, Altschuler SJ. Image-based multivariate profiling of drug responses from single cells. Nat Methods 2007;4(5):445-53
  • Park KM, Kang E, Jeon YJ, et al. Identification of novel regulators of apoptosis using a high-throughput cell-based screen. Mol Cells 2007;23(2):170-4
  • Prigozhina NL, Zhong L, Hunter EA, et al. Plasma membrane assays and three-compartment image cytometry for high content screening. Assay Drug Dev Technol 2007;5(1):29-48
  • Simpson JC, Cetin C, Erfle H, et al. An RNAi screening platform to identify secretion machinery in mammalian cells. J Biotechnol 2007;129:352-65
  • Tao CY, Hoyt J, Feng Y. A support vector machine classifier for recognizing mitotic subphases using high-content screening data. J Biomol Screen 2007;12(4):490-6
  • Vogt A, Lazo JS. Implementation of high-content assay for inhibitors of mitogen-activated protein kinase phosphatases. Methods 2007;42(3):268-77
  • Wang J, Xie X. Development of a quantitative, cell-based, high-content screening assay for epidermal growth factor receptor modulators. Acta Pharmacol Sin 2007;28(10):1698-704
  • Au Q, Kanchanastit P, Barber JR, et al. High-content image-based screening for small-molecule chaperone amplifiers in heat shock. J Biomol Screen 2008;13(10):953-9
  • Denner P, Schmalowsky J, Prechtl S. High-content analysis in preclinical drug discovery. Comb Chem High Throughput Screen 2008;11:216-30
  • Low J, Huang S, Blosser W, et al. High-content imaging characterization of cell cycle therapeutics through in vitro and in vivo subpopulation analysis. Mol Cancer Ther 2008;7(8):2455-63
  • Raccor BS, Vogt A, Sikorski RP, et al. Cell-based and biochemical structure-activity analyses of analogs of the microtubule stabilizer dictyostatin. Mol Pharmacol 2008;73(3):718-26
  • Sakaue-Sawano A, Kurokawa H, Morimura T, et al. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 2008;132:487-98
  • Shi J, Orth JD, Mitchison T. Cell type variation in responses to antimitotic drugs that target microtubules and kinesin-5. Cancer Res 2008;68(9):3269-76
  • Szafran AT, Szwarc M, Marcelli M, Mancini MA. Androgen receptor functional analyses by high throughput imaging: determination of ligand, cell cycle, and mutation-specific effects. PLoS ONE 2008;3(11):e3605
  • Young DW, Bender A, Hoyt J, et al. Integrating high-content screening and ligand-target prediction to identify mechanism of action. Nat Chem Biol 2008;4(1):59-68
  • Zhang B, Gu X, Uppalapati U, et al. High-content flluorescent-based assay for screening activators of DNA damage checkpoint pathways. J Biomol Screen 2008;13(6):538-43
  • Kau TR, Schroeder F, Ramaswamy S, et al. A chemical genetic screen identifies inhibitors of regulated nuclear export of a Forkhead transcription factor in PTEN-deficient tumor cells. Cancer Cell 2003;4:463-76
  • Almholt DL, Loechel F, Nielsen SJ, et al. Nuclear export inhibitors and kinase inhibitors identified using a MAPK-activated protein kinase2 redistribution(R) screening. Assay Drug Dev Technol 2004;2(1):7-20
  • Ghosh RN, DeBiasio R, Hudson CC, et al. Quantitative cell-based high-content screening for vasopressin receptor agonists using transfluor technology. J Biomol Screen 2005;10(5):476-84
  • Harada JN, Bower KE, Orth AP, et al. Identification of novel mammalian growth regulatory factors by genome-scale quantitative image analysis. Genome Res 2005;15(8):1136-44
  • Lundholt BK, Linde V, Loechel F, et al. Identification of Akt pathway inhibitors using redistribution screening on the FLIPR and the IN Cell 3000 Analyzer. J Biomol Screen 2005;10(1):20-9
  • Auld DS, Johnson RL, Zhang Y-Q, et al. Fluorescent protein-based cellular assays analyzed by laser-scanning microplate cytometry in 1536-well plate format. Methods Enzymol 2006;414:566-89
  • Duan Z, Bradner JE, Greenberg E, et al. SD-1029 inhibits signal transducer and activator of transcription 3 nuclear translocation. Clin Cancer Res 2006;12(22):6844-52
  • Garippa RJ, Hoffman AF, Gradl G, Kirsch A. High-throughput confocal microscopy for beta-arrestin-green fluorescent protein translocation G protein-coupled receptor assays using the Evotec Opera. Methods Enzymol 2006;414:99-120
  • Gasparri F, Cappella P, Galvani A. Multiparametric cell cycle analysis by automated microscopy. J Biomol Screen 2006;11(6):586-98
  • Grånäs C, Lundholt BK, Loechel F, et al. Identification of RAS-mitogen-activated protein kinase signaling pathway modulators in an ERF1 redistribution(R) screen. J Biomol Screen 2006;11(4):423-34
  • Howell BJ, Lee S, Sepp-Lorenzino L. Development and implementation of multiplexed cell-based imaging assays. Methods Enzymol 2006;414:284-300
  • Hudson CC, Oakley RH, Sjaastad MD, Loomis CR. High-content screening of known G protein-coupled receptors by arrestin translocation. Methods Enzymol 2006;414:63-78
  • Oakley RH, Hudson CC, Sjaastad MD, Loomis CR. The ligand-independent translocation assay: an enabling technology for screening orphan G protein-coupled receptors by arrestin recruitment. Methods Enzymol 2006;414:50-63
  • Soncini C, Carpinelli P, Gianellini L, et al. PHA-680632, a novel Aurora kinase inhibitor with potent antitumoral activity. Clin Cancer Res 2006;12(13):4080-9
  • Gasparri F, Ciavolella A, Galvani A. Cell-cycle inhibitor profiling by high-content analysis. Adv Exp Med Biol 2007;604:137-48
  • Loechel F, Bjørn S, Linde V, et al. High content translocation assays for pathway profiling. Methods Mol Biol 2007;356:401-14
  • Zanella F, Rosado A, Blanco F, et al. An HTS approach to screen for antagonists of the nuclear export machinery using high content cell-based assays. Assay Drug Dev Technol 2007;5(3):333-41
  • Cappella P, Gasparri F, Pulici M, Moll J. A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining. Cytometry A 2008;73(7):626-36
  • Ross DA, Lee S, Reiser V, et al. Multiplexed assays by high-content imaging for assessment of GPCR activity. J Biomol Screen 2008;13(6):449-55
  • Simonen M, Ibig-Rehm Y, Hofmann G, et al. High-content assay to study protein prenylation. J Biomol Screen 2008;13(6):456-67
  • Zanella F, Rosado A, García B, et al. Chemical genetic analysis of FOXO nuclear-cytoplasmic shuttling by using image-based cell screening. Chembiochem 2008;9(14):2229-37
  • Conway BR, Minor LK, Xu JZ, et al. Quantification of G-protein coupled receptor internalization using G-protein coupled receptor green fluorescent protein conjugate with the arrayscan high content screening system. J Biomol Screen 1999;4:75-86
  • Fowler A, Seifert N, Acker V, et al. A nonradioactive high-throughput/high-content assay for measurement of the human serotonin reuptake transporter function in vitro. J Biomol Screen 2006;11(8):1027-34
  • MacDonald ML, Lamerdin J, Owens S, et al. Identifying off-target effects and hidden phenotypes of drugs in human cells. Nat Chem Biol 2006;2(6):329-37
  • Michnick SW, MacDonald ML, Westwick JK. Chemical genetic strategies to delineate MAP kinase signaling pathways using protein-fragment complementation assays (PCA). Methods 2006;40:287-93
  • Teusch N, Kiefer C. A high-content screening assay for the Nogo receptor based on cellular Rho activation. Assay Drug Dev Technol 2006;4(2):133-41
  • Chan EYW, Kir S, Tooze SA. siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem 2007;282(35):25464-74
  • Rickardson L, Wickström M, Larsson R, Lövborg H. Image-based screening for the identification of novel proteasome inhibitors. J Biomol Screen 2007;12(2):203-10
  • Trzcinska-Daneluti A, Ly D, Huynh L, et al. High-content functional screen to identify proteins that correct F508del-CFTR function. Mol Cell Proteomics 2009;8(4):780-90
  • Bhawe KM, Blake RA, Clary DO, Flanagan PM. An automated image capture and quantitation approach to identify proteins affecting tumor cell proliferation. J Biomol Screen 2004;9(3):216-22
  • Giuliano KA, Chen Y-T, Lansing Taylor D. High-content screening with siRNA optimizes a cell biological approach to drug discovery: defining the role of p53 activation in the cellular response to anticancer drugs. J Biomol Screen 2004;9(7):557-68
  • Tanaka M, Bateman R, Rauh D, et al. An unbiased cell morphology-based screen for new, biologically active small molecules. PLoS Biol 2005;3(5):764-76
  • Grove LE, Ghosh RN. Quantitative characterization of mitosis-blocked tetraploid cells using high content analysis. Assay Drug Dev Technol 2006;4(4):421-42
  • Smellie A, Wilson C, Ng SC. Visualization and interpretation of high content screening data. J Chem Inf Model 2006;46:201-7
  • Wilson CJ, Si Y, Thompson CM, et al. Identification of a small molecule that induces mitotic arrest using a simplified high-content screening assay and data analysis method. J Biomol Screen 2006;11(1):21-8
  • Perlman ZE, Mitchison TJ, Mayer TU. High-content screening and profiling of drug activity in an automated centrosome-duplication assay. ChemBiochem 2005;6(1):145-51
  • Haasen D, Schnapp A, Valler MJ, Heilker R. G protein-coupled receptor internalization assays in the high-content screening format. Methods Enzymol 2006;414:121-39
  • Kawai A, Takano S, Nakamura N, Ohkuma S. Quantitative monitoring of autophagic degradation. Biochem Biophys Res Commun 2006;351(1):71-7
  • Lundholt BK, Heydorn A, Bjørn SP, Praestegaard M. A simple cell-based HTS assay system to screen for inhibitors of p53-Hdm2 protein-protein interactions. Assay Drug Dev Technol 2006;4(6):679-88
  • Wolff M, Haasen D, Merk S, et al. Automated high content screening for phosphoinositide 3 kinase inhibition using an AKT1 redistribution assay. Comb Chem High Throughput Screen 2006;9:339-50
  • Esposito A, Dohm CP, Bähr M, Wouters FS. Unsupervised fluorescence lifetime imaging microscopy for high content and high throughput screening. Mol Cell Proteomics 2007;6(8):1446-54
  • Haasen D, Merk S, Seither P, et al. Pharmacological profiling of chemochine receptor-directed compounds using high-content screening. J Biomol Screen 2008;13(1):40-53
  • Zhao X, Jones A, Olson KR, et al. A homogeneous enzyme fragment complementation-based beta-arrestin translocation assay for high-throughput screening of G-protein-coupled receptors. J Biomol Screen 2008;13(8):737-47
  • Pollice AA, Smith CA, Brown K, et al. Multiparameter analysis of human epithelial tumor cell lines by laser scanning cytometry. Cytometry 2000;42:347-356
  • DeBernardi MA, Brooker G. High-content kinetic calcium imaging in drug-sensitive and drug-resistant human breast cancer cells. Methods Enzymol 2006;414:317-35
  • Liu D, Wang L, Zhong R, et al. Parallel microfluidic networks for studying cellular response to chemical modulation. J Biotechnol 2007;131(3):286-92
  • Abraham VC, Samson B, Lapets O, Haskins JR. Automated classification of individual cellular responses across multiple targets. Preclinica 2004;2(5):349-55
  • O'Brien PJ, Irwin W, Diaz D, et al. High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening. Arch Toxicol 2006;80(9):580-604
  • Ye N, Qin J, Shi W, et al. Cell-based high content screening using an integrated microfluidic device. Lab Chip 2007;7(12):1696-704
  • Abraham VC, Towne DL, Waring JF, et al. Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans. J Biomol Screen 2008;13(6):527-37
  • Takanaga H, Chaudhuri B, Frommer WB. GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor. Biochim Biophys Acta 2008;1778(4):1091-9
  • Tencza SB, Sipe MA. Detection and classification of threat agents via high-content assays of mammalian cells. J Appl Toxicol 2004;24:371-7
  • Barabasz A, Foley B, Otto JC, et al. The use of high-content screening for the discovery and characterization of compounds that modulate mitotic index and cell cycle progression by differing mechanisms of action. Assay Drug Dev Technol 2006;4(2):153-63
  • Ross S, Chen T, Yu V, et al. High-content screening analysis of the p38 pathway: profiling of structurally related p38alpha kinase inhibitors using cell-based assays. Assay Drug Dev Technol 2006;4(4):397-409
  • Poon SSS, Wong JT, Sauders DN, et al. Intensity calibration and automated cell cycle gating for high-throughput image-based siRNA screens of mammalian cells. Cytometry A 2008;73(10):904-17
  • Moffat J, Grueneberg DA, Yang X, et al. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 2006;124:1283-98
  • Dürr O, Duval F, Nichols A, et al. Robust hit identification by quality assurance and multivariate data analysis of a high-content, cell-based assay. J Biomol Screen 2007;12(8):1042-9
  • Laketa V, Simpson JC, Bechtel S, et al. High-content microscopy identifies new neurite out growth regulators. Mol Biol Cell 2007;18:242-52
  • Gorjup E, Peter L, Wien S, et al. Automated microscopic quantification of adipogenic differentiation of human gland stem cells. Ann Anat 2009;191(1):13-22
  • Cheong R, Wang CJ, Levchenko A. High-content cell screening in a microfluidic device. Mol Cell Proteomics 2009;8:433-42
  • Morelli JK, Buehrle M, Pognan F, et al. Validation of an in vitro screen for phospholipidosis using a high-content biology platform. Cell Biol Toxicol 2006;22(1):15-27
  • Wagner BK, Carrinski HA, Ahn YH, et al. Small-molecule fluorophores to detect cell-state switching in the context of high-throughput screening. J Am Chem Soc 2008;130(13):4208-9
  • Dragunow M, Cameron R, Narayan P, O'Carroll S. Image-based high-throughput quantification of cellular fat accumulation. J Biomol Screen 2007;12(7):999-1005
  • Martinez ED, Dull AB, Beutler JA, Hager GL. High-content fluorescence-based screening for epigenetic modulators. Methods Enzymol 2006;414:21-36
  • Li Z, Yan Y, Powers EA, et al. Identification of gap junction blockers using automated fluorescence microscopy imaging. J Biomol Screen 2003;8(5):489-99
  • Evans GJ, Cousin MA. Simultaneous monitoring of three key neuronal functions in primary neuronal cultures. J Neurosci Methods 2007;160(2):197-205
  • Yarrow JC, Perlman ZE, Westwood NJ, Mitchison TJ. A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods. BMC Biotechnol 2004;4:21
  • Yarrow JC, Totsukawa G, Charras GT, Mitchison TJ. Screening for cell migration inhibitors via automated microscopy reveals a Rho-kinase inhibitor. Chem Biol 2005;12(3):385-95
  • Agler M, Prack M, Zhu Y, et al. A high-content glucocorticoid receptor translocation assay for compound mechanism-of-action evaluation. J Biomol Screen 2007;12(8):1029-41
  • Wang J, Zhou X, Bradley PL, et al. Cellular phenotype recognition for high-content RNA interference genome-wide screening. J Biomol Screen 2008;13(1):29-39
  • Jensen NA, Gerth K, Grotjohann T, et al. Establishment of a high content assay for the identification and characterization of bioactivities in crude bacterial extracts that interfere with the eukaryotic cell cycle. J Biotechnol 2009;140(1-2):124-34
  • Carpenter AE, Jones TR, Lamprecht MR, et al. CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol 2006;7(10):R100
  • Hill AA, LaPan P, Li Y, Haney S. Impact of image segmentation on high-content screening data quality for SK-BR-3 cells. BMC Bioinformatics 2007;8:340
  • To K, Zhao Y, Jiang H, et al. The phosphoinositide-dependent kinase-1 inhibitor 2-amino-N-[4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]phenyl]-acetamide (OSU-03012) prevents Y-box binding protein-1 from inducing epidermal growth factor receptor. Mol Pharmacol 2007;72(3):641-52
  • Li F, Zhou X, Zhu J, et al. High content image analysis for human H4 neuroglioma cells exposed to CuO nanoparticles. BMC Biotechnol 2007;7:66
  • Richards GR, Jack AD, Platts A, Simpson PB. Measurement and analysis of calcium signaling in heterogeneous cell cultures. Methods Enzymol 2006;414:335-47
  • Richards GR, Smith AJ, Parry F, et al. A morphology- and kinetics-based cascade for human neural cell high content screening. Assay Drug Dev Technol 2006;4(2):143-52
  • Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 1965;37(3):614-36
  • Gasparri F, Mariani M, Sola F, Galvani A. Quantification of the proliferation index of human dermal fibroblast cultures with the ArrayScanTM high-content screening reader. J Biomol Screen 2004;9(3):232-43
  • Borchert KM, Galvin RJ, Frolik CA, et al. High-content screening assay for activators of the Wnt/Fzd pathway in primary human cells. Assay Drug Dev Technol 2005;3(2):133-41
  • Wolf M, Kauschke SG, Schmidt S, Heilker R. Activation and translocation of glucokinase in rat primary hepatocytes monitored by high content image analysis. J Biomol Screen 2008;13(9):837-46
  • Viero C, Kraushaar U, Ruppenthal S, et al. A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventricular myocytes. Cell Calcium 2008;43(1):59-71
  • Pouton CW, Haynes JM. Embryonic stem cells as a source of models for drug discovery. Nat Rev Drug Discov 2007;6(8):605-16
  • Peerani R, Rao BM, Bauwens C, et al. Niche-mediated control of human embryonic stem cell self-renewal and differentiation. EMBO J 2007;26(22):4744-55
  • Walker E, Ohishi M, Davey RE, et al. Prediction and testing of novel transcriptional networks regulating embryonic stem cell self-renewal and commitment. Cell Stem Cell 2007;1(1):71-86
  • Rodriguez RA, Alfonso J, O'Day C, et al. 297 distinct cell lines: a high-content analysis assay and a full-automation design solely using noncontact liquid dispensing. JALA 2007;12:318-26
  • Giaccone G, Gadzar AF, Beck H, et al. Multidrug sensitivity phenotype of human lung cancer cells associated with topoisomerase II expression. Cancer Res 1992;52:1666-74
  • Vasudevan C, Fursov N, Maunder P, et al. Improving high-content-screening assay performance by using division-arrested cells. Assay Drug Dev Technol 2005;3(5):515-23
  • Digan ME, Pou C, Niu H, Zhang J-H. Evaluation of division-arrested cells for cell-based high-throughput screening and profiling. J Biomol Screen 2005;10(6):615-23
  • Lundholt BK, Scudder KM, Pagliaro L. A simple technique for reducing edge effect in cell-based assays. J Biomol Screen 2003;8:566-70
  • Kanda T, Sullivan KF, Wahl GM. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr Biol 1998;8(7):377-85
  • Diaz D, Scott A, Carmichael P, et al. Evaluation of an automated in vitro micronucleus assay in CHO-K1 cells. Mutat Res 2007;630(1-2):1-13
  • Li F, Zhou X, Ma J, Wong ST. An automated feedback system with the hybrid model of scoring and classification for solving over-segmentation problems in RNAi high content screening. J Microsc 2007;226:121-32
  • Harder N, Eils R, Rohr K. Automated classification of mitotic phenotypes of human cells using fluorescent proteins. Methods Cell Biol 2008;85:539-54
  • Available from: http://www.lgcstandards-atcc.org/
  • Masters JR. HeLa cells 50 years on: the good, the bad and the ugly. Nat Rev Cancer 2002;2(4):315-9
  • Nardone RM. Eradication of cross-contaminated cell lines: a call for action. Cell Biol Toxicol 2007;23(6):367-72

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