Emerging technologies in extracellular vesicle-based molecular diagnostics

References

• Perou CM, Sorlie T, Eisen MB, et al. Molecular portraits of human breast tumours. Nature 2000;406(6797):747-52
   PubMed Web of Science ®Google Scholar
• Sorlie T, Perou CM, Tibshirani R, et al. Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications. Proc Natl Acad Sci USA 2001;98(19):10869-74
   PubMed Web of Science ®Google Scholar
• Sorlie T, Tibshirani R, Parker J, et al. Repeated observation of breast tumor subtypes in independent gene expression data sets. Proc Natl Acad Sci USA 2003;100(14):8418-23
   PubMed Web of Science ®Google Scholar
• Habel LA, Shak S, Jacobs MK, et al. A population-based study of tumor gene expression and risk of breast cancer death among lymph node-negative patients. Breast Cancer Res 2006;8(3):R25
   PubMed Web of Science ®Google Scholar
• Paik S, Shak S, Tang G, et al. A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer. N Engl J Med 2004;351(27):2817-26
   PubMed Web of Science ®Google Scholar
• Buyse M, Loi S, van't Veer L, et al. Validation and clinical utility of a 70-gene prognostic signature for women with node-negative breast cancer. J Natl Cancer Inst 2006;98(17):1183-92
   PubMed Web of Science ®Google Scholar
• van de Vijver MJ, He YD, van't Veer LJ, et al. A gene-expression signature as a predictor of survival in breast cancer. N Engl J Med 2002;347(25):1999-2009
   PubMed Web of Science ®Google Scholar
• van 't Veer LJ, Dai H, van de Vijver MJ, et al. Gene expression profiling predicts clinical outcome of breast cancer. Nature 2002;415(6871):530-6
   PubMed Web of Science ®Google Scholar
• Foekens JA, Atkins D, Zhang Y, et al. Multicenter validation of a gene expression-based prognostic signature in lymph node-negative primary breast cancer. J Clin Oncol 2006;24(11):1665-71
   PubMed Web of Science ®Google Scholar
• Ring BZ, Seitz RS, Beck R, et al. Novel prognostic immunohistochemical biomarker panel for estrogen receptor-positive breast cancer. J Clin Oncol 2006;24(19):3039-47
   PubMed Web of Science ®Google Scholar
• Ma XJ, Hilsenbeck SG, Wang W, et al. The HOXB13:IL17BR expression index is a prognostic factor in early-stage breast cancer. J Clin Oncol 2006;24(28):4611-19
   PubMed Web of Science ®Google Scholar
• Ascierto PA, Kirkwood JM, Grob JJ, et al. The role of BRAF V600 mutation in melanoma. J Translat Med 2012;10:85
   PubMed Web of Science ®Google Scholar
• Nazarian R, Shi H, Wang Q, et al. Melanomas acquire resistance to B-RAF(V600E) inhibition by RTK or N-RAS upregulation. Nature 2010;468(7326):973-7
   PubMed Web of Science ®Google Scholar
• Momen-Heravi F, Balaj L, Alian S, et al. Current methods for the isolation of extracellular vesicles. Biol Chem 2013;394(10):1253-62
   PubMed Web of Science ®Google Scholar
• Momen-Heravi F, Balaj L, Alian S, et al. Alternative methods for characterization of extracellular vesicles. Front Physiol 2012;3:354
   PubMed Web of Science ®Google Scholar
• Valadi H, Ekstrom K, Bossios A, et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 2007;9(6):654-9
   PubMed Web of Science ®Google Scholar
• Mittelbrunn M, Gutierrez-Vazquez C, Villarroya-Beltri C, et al. Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. Nat Commun 2011;2:282
   PubMed Web of Science ®Google Scholar
• Skog J, Wurdinger T, van Rijn S, et al. Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 2008;10(12):1470-6
   PubMed Web of Science ®Google Scholar
• Ramachandran S, Palanisamy V. Horizontal transfer of RNAs: exosomes as mediators of intercellular communication. Wiley Interdiscip Rev RNA 2012;3(2):286-93
   PubMed Web of Science ®Google Scholar
• Tannous BA, Kim DE, Fernandez JL, et al. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther 2005;11(3):435-43
   PubMed Web of Science ®Google Scholar
• Lotvall J, Valadi H. Cell to cell signalling via exosomes through esRNA. Cell Adh Migr 2007;1(3):156-8
   PubMed Web of Science ®Google Scholar
• Feng D, Zhao WL, Ye YY, et al. Cellular internalization of exosomes occurs through phagocytosis. Traffic 2010;11(5):675-87
   PubMed Web of Science ®Google Scholar
• Morelli AE, Larregina AT, Shufesky WJ, et al. Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells. Blood 2004;104(10):3257-66
   PubMed Web of Science ®Google Scholar
• Svensson KJ, Christianson HC, Wittrup A, et al. Exosome uptake depends on ERK1/2-heat shock protein 27 signaling and lipid Raft-mediated endocytosis negatively regulated by caveolin-1. J Biol Chem 2013;288(24):17713-24
   PubMed Web of Science ®Google Scholar
• National Center for Advancing Translational Sciences. Available from: www.ncats.nih.gov/research/reengineering/exrna/exrna.html
   Google Scholar
• Kahlert C, Melo SA, Protopopov A, et al. Identification of double stranded genomic DNA spanning all chromosomes with mutated KRAS and p53 DNA in the serum exosomes of patients with pancreatic cancer. J Biol Chem 2014. [Epub ahead of print]
   PubMed Web of Science ®Google Scholar
• Huang X, Yuan T, Tschannen M, et al. Characterization of human plasma-derived exosomal RNAs by deep sequencing. BMC Genomics 2013;14:319
   PubMed Web of Science ®Google Scholar
• Dubyak GR. P2X7 receptor regulation of non-classical secretion from immune effector cells. Cell Microbiol 2012;14(11):1697-706
   PubMed Web of Science ®Google Scholar
• McKechnie NM, King BC, Fletcher E, Braun G. Fas-ligand is stored in secretory lysosomes of ocular barrier epithelia and released with microvesicles. Exp Eye Res 2006;83(2):304-14
   PubMed Web of Science ®Google Scholar
• Virgintino D, Rizzi M, Errede M, et al. Plasma membrane-derived microvesicles released from tip endothelial cells during vascular sprouting. Angiogenesis 2012;15(4):761-9
   PubMed Web of Science ®Google Scholar
• Matzdorff AC, Berchner D, Kuhnel G, et al. Relative and absolute changes of activated platelets, microparticles and platelet aggregates after activation in vitro. Haemostasis 1998;28(6):277-88
   PubMedGoogle Scholar
• Baj-Krzyworzeka M, Szatanek R, Weglarczyk K, et al. Tumour-derived microvesicles modulate biological activity of human monocytes. Immunol Lett 2007;113(2):76-82
   PubMed Web of Science ®Google Scholar
• Luga V, Zhang L, Viloria-Petit AM, et al. Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration. Cell 2012;151(7):1542-56
   PubMed Web of Science ®Google Scholar
• Peinado H, Aleckovic M, Lavotshkin S, et al. Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat Med 2012;18(6):883-91
   PubMed Web of Science ®Google Scholar
• Coltel N, Combes V, Wassmer SC, et al. Cell vesiculation and immunopathology: implications in cerebral malaria. Microbes Infect 2006;8(8):2305-16
   PubMed Web of Science ®Google Scholar
• Azevedo LC, Pedro MA, Laurindo FR. Circulating microparticles as therapeutic targets in cardiovascular diseases. Recent Pat Cardiovasc Drug Discov 2007;2(1):41-51
   PubMedGoogle Scholar
• Meng Y, Kang S, Fishman DA. Lysophosphatidic acid stimulates fas ligand microvesicle release from ovarian cancer cells. Cancer Immunol Immunother 2005;54(8):807-14
   PubMed Web of Science ®Google Scholar
• Gercel-Taylor C, Atay S, Tullis RH, et al. Nanoparticle analysis of circulating cell-derived vesicles in ovarian cancer patients. Anal Biochem 2012;428(1):44-53
   PubMed Web of Science ®Google Scholar
• Lima LG, Chammas R, Monteiro RQ, et al. Tumor-derived microvesicles modulate the establishment of metastatic melanoma in a phosphatidylserine-dependent manner. Canc Lett 2009;283(2):168-75
   PubMed Web of Science ®Google Scholar
• Grange C, Tapparo M, Collino F, et al. Microvesicles released from human renal cancer stem cells stimulate angiogenesis and formation of lung premetastatic niche. Cancer Res 2011;71(15):5346-56
   PubMed Web of Science ®Google Scholar
• Antwi-Baffour S, Kholia S, Aryee YK, et al. Human plasma membrane-derived vesicles inhibit the phagocytosis of apoptotic cells–possible role in SLE. Biochem Biophys Res Commun 2010;398(2):278-83
   PubMed Web of Science ®Google Scholar
• Wan S, Zhou Z, Duan B, Morel L. Direct B cell stimulation by dendritic cells in a mouse model of lupus. Arthritis Rheum 2008;58(6):1741-50
   PubMedGoogle Scholar
• Muller G. Microvesicles/exosomes as potential novel biomarkers of metabolic diseases. Diabetes Metab Syndr Obes 2012;5:247-82
   PubMedGoogle Scholar
• Gatti S, Bruno S, Deregibus MC, et al. Microvesicles derived from human adult mesenchymal stem cells protect against ischaemia-reperfusion-induced acute and chronic kidney injury. Nephrol Dial Transplant 2011;26(5):1474-83
   PubMed Web of Science ®Google Scholar
• Silverman JM, Clos J, de'Oliveira CC, et al. An exosome-based secretion pathway is responsible for protein export from Leishmania and communication with macrophages. J Cell Sci 2010;123(Pt 6):842-52
   PubMed Web of Science ®Google Scholar
• Ramakrishnaiah V, Thumann C, Fofana I, et al. Exosome-mediated transmission of hepatitis C virus between human hepatoma Huh7.5 cells. Proc Natl Acad Sci USA 2013;110(32):13109-13
   PubMed Web of Science ®Google Scholar
• Rahman MJ, Regn D, Bashratyan R, Dai YD. Exosomes released by islet-derived mesenchymal stem cells trigger autoimmune responses in NOD mice. Diabetes 2013. [Epub ahead of print]
   PubMed Web of Science ®Google Scholar
• Zhang HG, Liu C, Su K, et al. A membrane form of TNF-alpha presented by exosomes delays T cell activation-induced cell death. J Immunol 2006;176(12):7385-93
   PubMed Web of Science ®Google Scholar
• Taylor DD, Gercel-Taylor C. The origin, function, and diagnostic potential of RNA within extracellular vesicles present in human biological fluids. Front Genet 2013;4:142
   PubMedGoogle Scholar
• Thery C, Zitvogel L, Amigorena S. Exosomes: composition, biogenesis and function. Nat Rev Immunol 2002;2(8):569-79
   PubMed Web of Science ®Google Scholar
• Miller MC, Doyle GV, Terstappen LW. Significance of circulating tumor cells detected by the CellSearch system in patients with metastatic breast colorectal and prostate cancer. J Oncol 2010;617421
   PubMed Web of Science ®Google Scholar
• Miranda KC, Bond DT, McKee M, et al. Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease. Kidney Int 2010;78(2):191-9
   PubMed Web of Science ®Google Scholar
• Chen WW, Balaj L, Liau LM, et al. BEAMing and droplet digital PCR analysis of mutant IDH1 mRNA in glioma patient serum and cerebrospinal fluid extracellular vesicles. Mol Ther Nucleic Acids 2013;2:e109
   PubMedGoogle Scholar
• Arroyo JD, Chevillet JR, Kroh EM, et al. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci USA 2011;108(12):5003-8
   PubMed Web of Science ®Google Scholar
• Vickers KC, Remaley AT. Lipid-based carriers of microRNAs and intercellular communication. Curr Opin Lipidol 2012;23(2):91-7
   PubMed Web of Science ®Google Scholar
• Bryant RJ, Pawlowski T, Catto JW, et al. Changes in circulating microRNA levels associated with prostate cancer. Br J Cancer 2012;106(4):768-74
   PubMed Web of Science ®Google Scholar
• Mitchell PS, Parkin RK, Kroh EM, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA 2008;105(30):10513-18
   PubMed Web of Science ®Google Scholar
• Zhang HL, Yang LF, Zhu Y, et al. Serum miRNA-21: elevated levels in patients with metastatic hormone-refractory prostate cancer and potential predictive factor for the efficacy of docetaxel-based chemotherapy. Prostate 2011;71(3):326-31
   PubMed Web of Science ®Google Scholar
• Silva J, Garcia V, Rodriguez M, et al. Analysis of exosome release and its prognostic value in human colorectal cancer. Genes Chromosomes Cancer 2012;51(4):409-18
   PubMed Web of Science ®Google Scholar
• Taylor DD, Gercel-Taylor C. MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol 2008;110(1):13-21
   PubMed Web of Science ®Google Scholar
• Duijvesz D, Luider T, Bangma CH, Jenster G. Exosomes as biomarker treasure chests for prostate cancer. Eur Urol 2011;59(5):823-31
   PubMed Web of Science ®Google Scholar
• Dennis Lo YM, Chiu RW. Prenatal diagnosis: progress through plasma nucleic acids. Nat Rev Genet 2007;8(1):71-7
   PubMed Web of Science ®Google Scholar
• Diaz LA Jr. The current clinical value of genomic instability. Semin Cancer Biol 2005;15(1):67-71
   PubMed Web of Science ®Google Scholar
• Balaj L, Lessard R, Dai L, et al. Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences. Nat Commun 2011;2:180
   PubMed Web of Science ®Google Scholar
• Chen C, Skog J, Hsu CH, et al. Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip 2010;10(4):505-11
   PubMed Web of Science ®Google Scholar
• Thery C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol 2006; Chapter 3:Unit 3.22
   PubMedGoogle Scholar
• Tauro BJ, Greening DW, Mathias RA, et al. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods 2012;56(2):293-304
   PubMed Web of Science ®Google Scholar
• Momen-Heravi F, Balaj L, Alian S, et al. Impact of biofluid viscosity on size and sedimentation efficiency of the isolated microvesicles. Front Physiol 2012;3:162
   PubMed Web of Science ®Google Scholar
• Kalinina O, Lebedeva I, Brown J, Silver J. Nanoliter scale PCR with TaqMan detection. Nucleic Acids Res 1997;25(10):1999-2004
   PubMed Web of Science ®Google Scholar
• Vogelstein B, Kinzler KW. Digital PCR. Proc Natl Acad Sci USA 1999;96(16):9236-41
   PubMed Web of Science ®Google Scholar
• Day E, Dear PH, McCaughan F. Digital PCR strategies in the development and analysis of molecular biomarkers for personalized medicine. Methods 2013;59(1):101-7
   PubMed Web of Science ®Google Scholar
• Hindson BJ, Ness KD, Masquelier DA, et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 2011;83(22):8604-10
   PubMed Web of Science ®Google Scholar
• Warren L, Bryder D, Weissman IL, Quake SR. Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR. Proc Natl Acad Sci USA 2006;103(47):17807-12
   PubMed Web of Science ®Google Scholar
• Zhong Q, Bhattacharya S, Kotsopoulos S, et al. Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR. Lab Chip 2011;11(13):2167-74
   PubMed Web of Science ®Google Scholar
• Burgos KL, Javaherian A, Bomprezzi R, et al. Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing. RNA 2013;19(5):712-22
   PubMed Web of Science ®Google Scholar
• Kinde I, Wu J, Papadopoulos N, et al. Detection and quantification of rare mutations with massively parallel sequencing. Proc Natl Acad Sci USA 2011;108(23):9530-5
   PubMed Web of Science ®Google Scholar
• Cronin M, Ross JS. Comprehensive next-generation cancer genome sequencing in the era of targeted therapy and personalized oncology. Biomark Med 2011;5(3):293-305
   PubMed Web of Science ®Google Scholar
• Lipson D, Capelletti M, Yelensky R, et al. Identification of new ALK and RET gene fusions from colorectal and lung cancer biopsies. Nat Med 2012;18(3):382-4
   PubMed Web of Science ®Google Scholar
• Next-Generation Screening Goes National in UK. In: Cancer Discovery 2013;3:OF6
   Google Scholar
• Gerlinger M, Rowan AJ, Horswell S, et al. Intratumor heterogeneity and branched evolution revealed by multiregion sequencing. N Engl J Med 2012;366(10):883-92
   PubMed Web of Science ®Google Scholar
• Cortot AB, Italiano A, Burel-Vandenbos F, et al. KRAS mutation status in primary nonsmall cell lung cancer and matched metastases. Cancer 2010;116(11):2682-7
   PubMed Web of Science ®Google Scholar
• Higgins MJ, Jelovac D, Barnathan E, et al. Detection of tumor PIK3CA status in metastatic breast cancer using peripheral blood. Clin Cancer Res 2012;18(12):3462-9
   PubMed Web of Science ®Google Scholar
• Kuo WP, Liu F, Trimarchi J, et al. A sequence-oriented comparison of gene expression measurements across different hybridization-based technologies. Nat Biotechnol 2006;24(7):832-40
   PubMed Web of Science ®Google Scholar
• Liu F, Jenssen TK, Trimarchi J, et al. Comparison of hybridization-based and sequencing-based gene expression technologies on biological replicates. BMC Genomics 2007;8:153
   PubMed Web of Science ®Google Scholar
• Mantel PY, Hoang AN, Goldowitz I, et al. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe 2013;13(5):521-34
   PubMed Web of Science ®Google Scholar
• Lenassi M, Cagney G, Liao M, et al. HIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells. Traffic 2010;11(1):110-22
   PubMed Web of Science ®Google Scholar
• Hood JL, Pan H, Lanza GM, Wickline SA. Paracrine induction of endothelium by tumor exosomes. Lab Invest 2009;89(11):1317-28
   PubMed Web of Science ®Google Scholar
• Cho JA, Park H, Lim EH, et al. Exosomes from ovarian cancer cells induce adipose tissue-derived mesenchymal stem cells to acquire the physical and functional characteristics of tumor-supporting myofibroblasts. Gynecol Oncol 2011;123(2):379-86
   PubMed Web of Science ®Google Scholar
• Fevrier B, Vilette D, Archer F, et al. Cells release prions in association with exosomes. Proc Natl Acad Sci USA 2004;101(26):9683-8
   PubMed Web of Science ®Google Scholar
• Kim J, Morley S, Le M, et al. Enhanced shedding of extracellular vesicles from amoeboid prostate cancer cells: potential effects on the tumor microenvironment. Cancer Biol Ther 2014;15:4
   Google Scholar
• Que R, Ding G, Chen J, Cao L. Analysis of serum exosomal microRNAs and clinicopathologic features of patients with pancreatic adenocarcinoma. World J Surg Oncol 2013;11(1):219
   PubMed Web of Science ®Google Scholar
• Gourzones C, Gelin A, Bombik I, et al. Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells. Virol J 2010;7:271
   PubMed Web of Science ®Google Scholar
• Aushev VN, Zborovskaya IB, Laktionov KK, et al. Comparisons of microRNA patterns in plasma before and after tumor removal reveal new biomarkers of lung squamous cell carcinoma. PLoS One 2013;8(10):e78649
   PubMed Web of Science ®Google Scholar
• Xiao D, Ohlendorf J, Chen Y, et al. Identifying mRNA, microRNA and protein profiles of melanoma exosomes. PLoS One 2012;7(10):e46874
   PubMed Web of Science ®Google Scholar
• Bellingham SA, Coleman BM, Hill AF. Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells. Nucleic Acids Res 2012;40(21):10937-49
   PubMed Web of Science ®Google Scholar
• Nilsson J, Skog J, Nordstrand A, et al. Prostate cancer-derived urine exosomes: a novel approach to biomarkers for prostate cancer. Br J Cancer 2009;100(10):1603-7
   PubMed Web of Science ®Google Scholar
• Lv LL, Cao YH, Ni HF, et al. MicroRNA-29c in urinary exosome/microvesicle as a biomarker of renal fibrosis. Am J Physiol Renal Physiol 2013;305(8):F1220-7
   PubMed Web of Science ®Google Scholar
• Noerholm M, Balaj L, Limperg T, et al. RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls. BMC Cancer 2012;12:22
   PubMed Web of Science ®Google Scholar
• Li CC, Eaton SA, Young PE, et al. Glioma microvesicles carry selectively packaged coding and non-coding RNAs which alter gene expression in recipient cells. RNA Biol 2013;10(8):1333-44
   PubMed Web of Science ®Google Scholar
• Corcoran C, Friel AM, Duffy MJ, et al. Intracellular and extracellular microRNAs in breast cancer. Clin Chem 2011;57(1):18-32
   PubMed Web of Science ®Google Scholar
• Michael A, Bajracharya SD, Yuen PS, et al. Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis 2010;16(1):34-8
   PubMed Web of Science ®Google Scholar
• Lu S, Mukkada VA, Mangray S, et al. MicroRNA profiling in mucosal biopsies of eosinophilic esophagitis patients pre- and post-treatment with steroids and relationship with mRNA targets. PLoS One 2012;7(7):e40676
   PubMed Web of Science ®Google Scholar
• Chiba M, Kimura M, Asari S. Exosomes secreted from human colorectal cancer cell lines contain mRNAs, microRNAs and natural antisense RNAs, that can transfer into the human hepatoma HepG2 and lung cancer A549 cell lines. Oncol Rep 2012;28(5):1551-8
   PubMed Web of Science ®Google Scholar
• Silva J, Garcia V, Zaballos A, et al. Vesicle-related microRNAs in plasma of nonsmall cell lung cancer patients and correlation with survival. Eur Respir J 2011;37(3):617-23
   PubMed Web of Science ®Google Scholar
• Rekker K, Saare M, Roost AM, et al. Comparison of serum exosome isolation methods for microRNA profiling. Clin Biochem 2014;47(1-2):135-8
   PubMed Web of Science ®Google Scholar
• Akers JC, Ramakrishnan V, Kim R, et al. miR-21 in the Extracellular Vesicles (EVs) of Cerebrospinal Fluid (CSF): a platform for glioblastoma biomarker development. PLoS One 2013;8(10):e78115
   PubMed Web of Science ®Google Scholar
• Ekstrom K, Valadi H, Sjostrand M, et al. Characterization of mRNA and microRNA in human mast cell-derived exosomes and their transfer to other mast cells and blood CD34 progenitor cells. J Extracell Vesicles 2012;1
   PubMedGoogle Scholar
• Camacho L, Guerrero P, Marchetti D. MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. PLoS One 2013;8(9):e73790
   PubMed Web of Science ®Google Scholar
• Witwer KW, McAlexander MA, Queen SE, Adams RJ. Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little evidence for general uptake of dietary miRNAs: limited evidence for general uptake of dietary plant xenomiRs. RNA Biol 2013;10(7):1080-6
   PubMed Web of Science ®Google Scholar
• Gildea JJ, Carlson JM, Schoeffel CD, et al. Urinary exosome miRNome analysis and its applications to salt sensitivity of blood pressure. Clin Biochem 2013;46(12):1131-4
   PubMed Web of Science ®Google Scholar
• Batagov AO, Kurochkin IV. Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3'-untranslated regions. Biol Direct 2013;8:12
   PubMed Web of Science ®Google Scholar
• Zhou Q, Li M, Wang X, et al. Immune-related microRNAs are abundant in breast milk exosomes. Int J Biol Sci 2012;8(1):118-23
   PubMed Web of Science ®Google Scholar
• Gu Y, Li M, Wang T, et al. Lactation-related microRNA expression profiles of porcine breast milk exosomes. PLoS One 2012;7(8):e43691
   PubMed Web of Science ®Google Scholar